Heliyon, Год журнала: 2024, Номер 10(24), С. e40988 - e40988
Опубликована: Дек. 1, 2024
Язык: Английский
Bioorganic Chemistry, Год журнала: 2025, Номер unknown, С. 108192 - 108192
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
3
Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160470 - 160470
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
2
Heliyon, Год журнала: 2024, Номер 10(14), С. e34692 - e34692
Опубликована: Июль 1, 2024
The emergence and development of antibiotic resistance in bacteria is a serious threat to global public health. Antibiotic genes (ARGs) are often located on mobile genetic elements (MGEs). They can be transferred among by horizontal gene transfer (HGT), leading the spread drug-resistant strains treatment failure. CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated genes) one many strategies have developed under long-term selection pressure restrict HGT. CRISPR-Cas systems exist about half bacterial genomes play significant role limiting resistance. On other hand, bacteriophages MGEs encode wide range anti-CRISPR proteins (Acrs) counteract immunity system. Acrs could decrease system's activity against phages facilitate acquisition ARGs virulence traits for bacteria. This review aimed assess relationship between with We also highlighted technology control prevent antibacterial system target nucleic acid sequences high accuracy reliability; therefore, it has become novel editing therapy tool CRISPR-based approaches may pave way developing smart antibiotics, which eliminate multidrug-resistant (MDR) distinguish pathogenic beneficial microorganisms. Additionally, engineered gene-containing combination antibiotics used as cutting-edge approach reduce
Язык: Английский
Процитировано
9
Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14
Опубликована: Июнь 26, 2024
Antibiotic resistance, a known global health challenge, involves the flow of bacteria and their genes among animals, humans, surrounding environment. It occurs when evolve become less responsive to drugs designated kill them, making infections harder treat. Despite several obstacles preventing spread bacteria, pathogens regularly acquire novel resistance factors from other species, which reduces ability prevent treat such bacterial infections. This issue requires coordinated efforts in healthcare, research, public awareness address its impact on human worldwide. review outlines how recent advances gene editing technology, especially CRISPR/Cas9, unveil breakthrough combating antibiotic resistance. Our focus will remain relationship between CRISPR/cas9 related Moreover, prospects this new advanced research challenges adopting these technologies against be outlined by exploring different derivatives discussing advantages limitations over others, thereby providing corresponding reference for control prevention
Язык: Английский
Процитировано
7
Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2024, Номер unknown
Опубликована: Окт. 15, 2024
Язык: Английский
Процитировано
5
Colloids and Surfaces B Biointerfaces, Год журнала: 2024, Номер 248, С. 114478 - 114478
Опубликована: Дек. 28, 2024
Язык: Английский
Процитировано
4
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 10185 - 10212
Опубликована: Окт. 1, 2024
Abstract: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated sequence (CRISPR/Cas) system is a cutting-edge genome-editing tool employed to explore the functions of normal and disease-related genes. CRISPR/Cas has remarkable diversity in composition architecture genomic loci Cas protein sequences. Owing its excellent efficiency specificity, this adds an outstanding dimension biomedical research on genetic manipulation eukaryotic cells. However, safe, efficient, specific delivery target cells tissues their off-target effects are considered critical bottlenecks for therapeutic applications. Recently discovered anti-CRISPR proteins (Acr) play significant role limiting system. Acrs relatively small that highly CRISPR variants exhibit structural diversity. silico approaches, crystallography, cryo-electron microscopy roles elucidating mechanisms action Acrs. block mainly by employing four mechanisms: complex assembly interruption, target-binding interference, cleavage prevention, degradation cyclic oligonucleotide signaling molecules. Engineered systems frequently used gene therapy, diagnostics, functional genomics. Understanding molecular underlying Acr may help safe effective use tools modification, particularly context medicine. Thus, attempts regulate prokaryotic surveillance complexes will advance development antimicrobial drugs treatment human diseases. In review, recent updates systems, especially CRISPR/Cas9 Acrs, novel mechanistic insights elaborated. addition, applications CRISPP/Cas biotechnology precise genome editing other discussed. Keywords: CRISPR/Cas, proteins, editing, enzyme inhibition, inhibitors,
Язык: Английский
Процитировано
3
Reports of Vinnytsia National Medical University, Год журнала: 2025, Номер 29(1), С. 29 - 34
Опубликована: Март 20, 2025
Annotation. The global threat of bacterial resistance to antibiotics necessitates the search for alternatives and methods overcome resistance. aim this study was investigate antibacterial effect low-intensity electric current on multidrug-resistant P. aeruginosa strains determine its impact susceptibility antibiotics. studied reference clinical (n=15) in isotonic solution meat-peptone broth (MPB), with concentration measured using a densitometer. after exposure physical factor assessed serial dilution method minimum inhibitory (MIC) bactericidal (MBC). arithmetic mean (M), standard error (m), statistical significance (p) were calculated. As result solution, significant reduction observed compared untreated control: 1.3-fold decrease at 24 hours (p≤0.05) 2.38-fold 48 (p≤0.01). In MPB conditions, led cell by 1.36-fold (p ≤ 0.05) 1.42-fold hours. treatment reduced antibiotic 2- 4-fold. Without prior exposure, average MIC values ceftazidime 156.25±31.25 µg/mL, MBC - 479.17±122.55 µg/mL. For cefepime, 187.5±68.47 µg/mL 520.83±159.48 respectively. significantly decreased (p≤0.001) approximately 2.67-fold, 3.33-fold. cefepime 3.67-fold, 4.67-fold. resistant under influence currents expands understanding potential use weaken adaptive properties provides additional opportunities combating pathogen responsible infectious complications.
Язык: Английский
Процитировано
0
Methods in cell biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Methods, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0