Biochemistry and Biophysics Reports, Год журнала: 2024, Номер 40, С. 101877 - 101877
Опубликована: Ноя. 17, 2024
Язык: Английский
Microbial Pathogenesis, Год журнала: 2025, Номер unknown, С. 107370 - 107370
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
1
Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Год журнала: 2025, Номер unknown, С. 189263 - 189263
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Pharmaceutics, Год журнала: 2025, Номер 17(3), С. 331 - 331
Опубликована: Март 4, 2025
Background/Objectives: Natural substances have been a widely studied source of both pharmaceutical excipients and drugs. Berberine (BRB) is benzylisoquinoline alkaloid isolated from different plant sources. It possesses various pharmacological properties including antibacterial, antitumor, antidiabetic, neuroprotective, hepatoprotective, anti-inflammatory, antioxidant, etc. However, the limited aqueous solubility hinders its application. Nanosized drug delivery systems are an innovative approach for addressing challenges regarding via routes administration. Their utilization could improve active constituents. Methods: A melt-emulsification ultrasonication technique was applied preparation nanostructured lipid carriers (NLCs). They were thoroughly physicochemically characterized by means Dynamic Light Scattering, TEM, FTIR, DSC, TGA, In Vitro release. The efficacy safety evaluated on cholangiocarcinoma, colorectal adenocarcinoma, hepatocellular carcinoma, lymphoma, fibroblast, cardioblast cells, as well rat liver microsomes cytotoxicity assays comet assay. Results: obtained nanoparticles had spherical shape size around 158.2 ± 1.8 nm with negative zeta potential. revealed successful loading improved dissolution berberine in physiological conditions. studies showed that BRB NLCs resulted or retained to tumor cell lines reduced normal microsomes. NLC itself increased microsomal malondialdehyde (MDA) formation. Conclusions: presented. nanocarriers show favorable physicochemical biopharmaceutical properties. cellular experiments anticancer safety. These findings highlight potential applicability gastrointestinal neoplasms build foundation future practical translation.
Язык: Английский
Процитировано
0
Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер unknown, С. 106924 - 106924
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Cell Biology International, Год журнала: 2025, Номер unknown
Опубликована: Май 30, 2025
ABSTRACT Berberine (BBR), one of the main active isoquinoline alkaloids in Coptis chinensis , has gradually gained attention for its therapeutic effect on various tumors, including colorectal cancer (CRC). However, detailed mechanisms underlying remain to be elucidated. The cytotoxic potential BBR towards CRC cells was examined by MTT. Autophagy indicated acidic vesicular organelle formation, LC3 dots accumulation and conversion LC3I LC3II, while autophagic flux monitored fusion autophagosomes lysosomes based green fluorescence quenching transfected with mRFP‐GFP‐LC3 plasmids P62 degration. Furthermore, endoplasmic reticulum (ER) stress response‐associated proteins cell lines treated and/or ER inhibitor 4‐Phenylbutyric acid (4‐BPA) were assessed Western blotting. BBR‐induced intracellular ROS measured DCFH‐DA. ZIP Synergy scores calculated using Synergyfinder software evaluate synergistic effects 5‐fluorouracil (5‐FU). induced death autophagy‐dependent resulted sustained oxidative stress, which contributed induction complete flux. inhibition autophagy chloroquine (CQ) partially reversed anticancer BBR, suggesting that autophagy. Importantly, enhanced sensitivity 5‐FU.BBR might a novel chemotherapy adjuvant drug targets regulating stress/oxidative stress/autophagy/apoptosis.
Язык: Английский
Процитировано
0
Biochemistry and Biophysics Reports, Год журнала: 2024, Номер 40, С. 101877 - 101877
Опубликована: Ноя. 17, 2024
Язык: Английский
Процитировано
2