Sijunzi decoction alleviates inflammation and intestinal epithelial barrier damage and modulates the gut microbiota in ulcerative colitis mice

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Апрель 8, 2024

Ethnopharmacological relevance: As a representative classical prescription, Sijunzi decoction has powerful therapeutic effects on spleen–stomach qi insufficiency. Ulcerative colitis (UC) is chronic, diffuse, and non-specifically inflammatory disorder, the etiology of which still remains unclear. In traditional Chinese medicine (TCM) perspective, splenic asthenia primary cause UC. Based this, been extensively used in TCM clinical practice to alleviate UC recent years. However, pharmacological mechanism modern not completely clear, limits its application. Aim study: The purpose this study was investigate decoction’s curative effect acute mice probe into potential mechanism. Materials methods: mouse model set up by freely ingesting 3% dextran sulfate sodium (DSS) solution. relieving role analyzed evaluating changes parameters, colon morphology, histopathology, factor content, intestinal epithelial barrier protein expression level, gut microbiota balance state. Finally, multivariate statistical analysis conducted elucidate relationship between factors, proteins, microbiota. Results: First, research findings revealed that could visibly ease manifestation UC, lower DAI score, attenuate colonic damage. Moreover, also significantly inhibit IL-6, IL-1β, TNF-α while increasing occludin ZO-1 levels. Subsequently, further studies showed remodel homeostasis. beneficial regulating levels Alistipes, Akkermansia, Lachnospiraceae_NK4A136_group, other bacteria. demonstrated key microbes were closely associated with factors proteins. Conclusion: can prevent treat Its strongly improvement inflammation damage

Язык: Английский

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Huaier Polysaccharide Alleviates Dextran Sulphate Sodium Salt-Induced Colitis by Inhibiting Inflammation and Oxidative Stress, Maintaining the Intestinal Barrier, and Modulating Gut Microbiota

Nutrients, Год журнала: 2024, Номер 16(9), С. 1368 - 1368

Опубликована: Апрель 30, 2024

The incidence of ulcerative colitis (UC) is increasing annually, and UC has a serious impact on patients’ lives. Polysaccharides have gained attention as potential drug candidates for treating in recent years. Huaier (Trametes robiniophila Murr) fungus that been used clinically more than 1000 years, its bioactive polysaccharide components reported to possess immunomodulatory effects, antitumour potential, renoprotective effects. In this study, we aimed examine the protective effects mechanisms (HP) against UC. Based H2O2-induced oxidative stress model HT-29 cells dextran sulphate sodium salt (DSS)-induced model, demonstrated polysaccharides significantly alleviated DSS-induced (weight loss, elevated disease activity index (DAI) scores, colonic shortening). addition, HP inhibited inflammation intestinal barrier damage. It also promoted expression mucin Muc2. Furthermore, reduced abundance harmful bacteria Escherichia-Shigella beneficial Muribaculaceae_unclassified, Anaerotruncus, Ruminococcaceae_unclassified regulate flora disturbance caused by DSS. Nontargeted metabolomics revealed intervention would modulate metabolism promoting levels 3-hydroxybutyric acid, phosphatidylcholine (PC), phosphatidylethanolamine (PE). These results had ability mitigate suppressing inflammation, maintaining barrier, modulating flora. findings will expand our knowledge how functions offer theoretical foundation using prebiotic prevent

Язык: Английский

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Potential therapeutic target for polysaccharide inhibition of colon cancer progression

Frontiers in Medicine, Год журнала: 2024, Номер 10

Опубликована: Янв. 8, 2024

In recent years, colon cancer has become one of the most common malignant tumors worldwide, posing a great threat to human health. Studies have shown that natural polysaccharides rich biological activities and medicinal value, such as anti-inflammatory, anti-cancer, anti-oxidation, immune-enhancing effects, especially with potential anti-colon mechanisms. Natural can not only protect enhance homeostasis intestinal environment but also exert direct inhibition effect on cells, making it promising strategy for treating cancer. Preliminary clinical experiments demonstrated oral administration low high doses citrus pectin reduce tumor volume in mice by 38% (

Язык: Английский

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Effects of traditional Chinese medicine polysaccharides on chronic diseases by modulating gut microbiota: A review

International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 136691 - 136691

Опубликована: Окт. 1, 2024

Язык: Английский

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Potential for the development of Taraxacum mongolicum aqueous extract as a phytogenic feed additive for poultry

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Март 11, 2024

Introduction Taraxacum mongolicum (TM) is a kind of medicinal and edible homologous plant which included in the catalogue feed raw materials China. It rich polyphenols, flavonoids, polysaccharides other active substances, shows many benefits to livestock, poultry aquatic products. The study aimed assess potential TM aqueous extract (TMAE) as substitute for AGPs. Methods A total 240 one-day-old Arbor Acker broilers were randomly assigned four groups fed basal diet (Con) supplemented with 500, 1000, 2000 mg/kg TMAE (Low, Medium, High groups). growth performance was measured on day 21 42. At end trial, researchers slaughter collected serum, liver, spleen, ileum, intestinal contents investigate effects serum biochemistry, antioxidant capacity, immune function, organ coefficient, morphology, flora composition, short-chain fatty acids (SCFAs). Results results showed that treated had significantly higher average daily gain from 22 42 days old compared Con group. Various doses resulted different levels improvement chemistry. increased alkaline phosphatase decreased creatinine. also capacity ileum broilers. Additionally, middle high enhanced innate function liver (IL-10) (Occludin) Compared control group, treatment group exhibited an increase ratio villi length crypt duodenum. abundance beneficial bacteria, such Alistipes Lactobacillus, while reducing accumulation harmful Colidextracter Sellimonas . cecum's SCFAs content medium dose TMAE. Supplementing broiler diets at varying overall health. most significant observed 1000 mg/kg, including improved biochemical parameters, content. Lactobacillus aviarius, norank_f_norank_o__Clostridia_UCG-014 , Flavonifractor are potentially dominant members microflora. Conclusion In conclusion, promising additive effective reference dose.

Язык: Английский

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Enhanced anti-inflammatory activity of chlorogenic acid via folic acid-TPGS-modified liposomes encapsulation: characterization and In vivo evaluation on colitis mice

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Авг. 23, 2024

Introduction Chlorogenic acid (CGA) has been identified to possess salient anti-inflammatory, antioxidant, and anticancer attributes. However, its application is limited by instability low bioavailability. Liposomes have considered effective pharmaceutical delivery vehicles due their ability continuously release loaded drugs, improve drug stability, display good biocompatibility. They can be easily modified other small molecules acquire additional biological functions. In this study, we developed characterized folic acid-TPGS-modified chlorogenic liposome (FTCLP) evaluated anti-inflammatory activity. Methods The successful encapsulation of CGA within FTCLP was confirmed through examination using electron microscopy, fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC). in vitro characteristics were the dialysis bag membrane method. Meanwhile, a dextran sulfate sodium (DSS) -induced colitis model employed investigate effect mechanism. Results exhibited an efficiency (EE) 84.85 ± 1.20% loading (DL) 11.67 0.04%. particle size determined 150.63 0.71 nm, with polydispersity index (PDI) 0.198 0.02 zeta potential 2.61 0.38 mV. profile followed Higuchi model, indicating sustained-release characteristics. vivo study demonstrated that treatment improving symptoms DSS-induced inflammatory response, as evidenced mitigation weight loss, reduction disease activity (DAI) score, restoration colon length, attenuation tissue damage. Furthermore, levels pro-inflammatory cytokines, including interferon-gamma (INF-γ), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), markedly diminished both serum tissue. also observed suppress expression INF-γ , IL-1β IL-6 tumor necrosis factor-alpha ( TNF-α ), nuclear factor kappa B NF-κB ) p65 while concomitantly upregulating Janus kinase JAK signal transducer activator transcription 3 STAT3 ). Besides, administration found result increase abundance Lactobacillaceae Peptostreptococcaceae decreasing Bacteroidaceae Rikenellaceae Helicobacteraceae . Conclusion Following liposomes, revealed favorable stability sustained properties, enhanced effects modulating multiple inflammation-related biomarkers. safe for targeted therapy colitis.

Язык: Английский

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Type 5 Resistant Starch Can Effectively Alleviate Experimentally Induced Colitis in Mice by Modulating Gut Microbiota

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 5, 2024

Resistant starch (RS) has been shown to modulate intestinal microbiota in animal models ways that could reduce the effects of dysbiosis-related diseases. However, mechanism how this is achieved not understood. The present study aimed reveal RS mitigates dextran sulfate sodium (DSS)-induced colitis mice by using a starch-lipid complex (RS type 5), with an 2 from high-amylose maize as comparison. Both RS5 and RS2 induced changes diversity composition gut bacteria, leading alleviation symptoms including decreasing loss body weight, disease activity index score, colonic shortening. levels inflammatory cytokines were modulated accompanied increase goblet cell numbers thickening mucus layer. was more effective, compared RS2, alleviating all symptoms, mainly through improving microflora dysbiosis stimulating generation short-chain fatty acids (SCFAs). Our shows effectively alleviate colitis, highlighting potential use for RS5, particularly relieving bowel disease.

Язык: Английский

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Verrucomicrobia: Akkermansia

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 347 - 377

Опубликована: Янв. 1, 2025

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Hericium erinaceus Extract Exerts Beneficial Effects on Gut–Neuroinflammaging–Cognitive Axis in Elderly Mice

Biology, Год журнала: 2023, Номер 13(1), С. 18 - 18

Опубликована: Дек. 28, 2023

Ageing is a biological phenomenon that determines the impairment of cognitive performances, in particular, affecting memory. Inflammation and cellular senescence are known to be involved pathogenesis decline. The gut microbiota–brain axis could exert critical role influencing brain homeostasis during ageing, modulating neuroinflammation, possibly leading inflammaging. Due their anti-ageing properties, medicinal mushrooms can utilised as resource for developing pharmaceuticals functional foods. Specifically, Hericium erinaceus (He), thanks its bioactive metabolites, exerts numerous healthy beneficial effects, such reinforcing immune system, counteracting improving performance. Our previous works demonstrated capabilities two months He1 standardised extract oral supplementation preventing decline elderly frail mice. Herein, we showed this treatment did not change overall microbiome composition but significantly modified relative abundance genera specifically cognition inflammation. Parallelly, significant decrease crucial markers inflammation senescence, i.e., CD45, GFAP, IL6, p62, γH2AX, was dentate gyrus Cornus Ammonis hippocampal areas through immunohistochemical experiments. In summary, suggested anti-inflammatory properties mouse hippocampus microbiome–brain modulation.

Язык: Английский

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Modified Gegen Qinlian Decoction modulated the gut microbiome and bile acid metabolism and restored the function of goblet cells in a mouse model of ulcerative colitis

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Авг. 6, 2024

Modified Gegen Qinlian Decoction (MGQD) has been shown to effectively relieve ulcerative colitis (UC) without a known pharmacological mechanism. In this study, the anti-colitis efficaciousness of MGQD and its underlying mechanisms in UC were evaluated.

Язык: Английский

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