PDGF-BB improves cortical bone quality through restoring the osteogenic microenvironment in the steroid-associated osteonecrosis of rabbits

Journal of Orthopaedic Translation, Год журнала: 2025, Номер 52, С. 97 - 115

Опубликована: Апрель 12, 2025

Язык: Английский

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The Function and Mechanism of Anti-Inflammatory Factor Metrnl Prevents the Progression of Inflammatory-Mediated Pathological Bone Osteolytic Diseases

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 1607 - 1619

Опубликована: Март 1, 2024

Abstract: Metrnl, recently identified as an adipokine, is a secreted protein notably expressed in white adipose tissue, barrier tissues, and activated macrophages. This adipokine plays pivotal role counteracting obesity-induced insulin resistance. It enhances tissue functionality by promoting adipocyte differentiation, activating metabolic pathways, exerting anti-inflammatory effects. Extensive research has Metrnl key player modulating inflammatory responses integral regulator of muscle regeneration. These findings position promising biomarker potential therapeutic target treating inflammation-associated pathologies. Despite this, the specific mechanisms immune-mediated osteolysis arthritis remain elusive, warranting further investigation. In this review, we will briefly elaborate on anti-inflammation function inflammation-related osteolysis, arthritis, pathological bone resorption, which could facilitate Metrnl's clinical application novel strategy to prevent loss. While pathogenesis elbow stiffness remains current literature suggests that likely exerts its development. Keywords: loss, stiff

Язык: Английский

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m6A methylation modification and immune infiltration analysis in osteonecrosis of the femoral head

Journal of Orthopaedic Surgery and Research, Год журнала: 2024, Номер 19(1)

Опубликована: Март 15, 2024

Abstract Osteonecrosis of the femoral head (ONFH) is a elaborate hip disease characterized by collapse and osteoarthritis. RNA N6-methyladenosine (m6A) plays crucial role in lot biological processes within eukaryotic cells. However, m6A regulation ONFH remains unclear. In this study, we identified regulators performed subtype classification. We 7 significantly differentially expressed through analysis differences between normal samples Gene Expression Omnibus (GEO) database. A random forest algorithm was employed to monitor these assess risk developing ONFH. constructed nomogram based on regulators. The decision curve suggested that patients can benefit from model. classified into two models according consensus clustering algorithm. After that, evaluated those patterns using principal component analysis. assessed scores their relationship with immune infiltration. observed higher score type than B. Finally, cross-validation regulatory factors external datasets bone samples. conclusion, believed pattern could provide novel diagnostic strategy offer new insights for molecularly targeted therapy

Язык: Английский

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Interactions and Trends of Interleukins, PAI-1, CRP, and TNF-α in Inflammatory Responses during the Perioperative Period of Joint Arthroplasty: Implications for Pain Management—A Narrative Review

Journal of Personalized Medicine, Год журнала: 2024, Номер 14(5), С. 537 - 537

Опубликована: Май 17, 2024

Inflammation during the perioperative period of joint arthroplasty is a critical aspect patient outcomes, influencing both pathophysiology pain and healing process. This narrative review comprehensively evaluates roles specific cytokines inflammatory biomarkers in this context their implications for management. Inflammatory responses are initiated propagated by cytokines, which pivotal development acute chronic postoperative pain. Pro-inflammatory play essential up-regulating response, which, if not adequately controlled, leads to sustained impaired tissue healing. Anti-inflammatory work dampen promote resolution. Our discussion extends genetic molecular influences on cytokine production, influence perception recovery rates post-surgery. Furthermore, role PAI-1 modulating inflammation through its impact fibrinolytic system highlights potential as therapeutic target. The modulation these various analgesic anesthetic techniques, including fascia iliac compartment block, demonstrates significant reduction markers, thus underscoring importance targeted strategies. analysis suggests that nuanced understanding interplay between pro-inflammatory anti-inflammatory required. Future research should focus individualized management

Язык: Английский

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Toll-like receptors in cardiac hypertrophy

Frontiers in Cardiovascular Medicine, Год журнала: 2023, Номер 10

Опубликована: Апрель 11, 2023

Toll-like receptors (TLRs) are a family of pattern recognition (PRRs) that can identify pathogen-associated molecular patterns (PAMPs) and damage-associated (DAMPs). TLRs play an important role in the innate immune response, leading to acute chronic inflammation. Cardiac hypertrophy, cardiac remodeling phenotype during cardiovascular disease, contributes development heart failure. In previous decades, many studies have reported TLR-mediated inflammation was involved induction myocardium hypertrophic remodeling, suggesting targeting TLR signaling might be effective strategy against pathological hypertrophy. Thus, it is necessary study mechanisms underlying functions this review, we summarized key findings

Язык: Английский

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Exosomal miR-1a-3p derived from glucocorticoid-stimulated M1 macrophages promotes the adipogenic differentiation of BMSCs in glucocorticoid-associated osteonecrosis of the femoral head by targeting Cebpz

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Окт. 22, 2024

By interacting with bone marrow mesenchymal stem cells (BMSCs) and regulating their function through exosomes, macrophages play crucial roles in various bone-related diseases. Research has highlighted a notable increase the number of M1 glucocorticoid-associated osteonecrosis femoral head (GA-ONFH). Nevertheless, intricate crosstalk between BMSCs glucocorticoid-stimulated environment not been fully elucidated, underlying regulatory mechanisms involved occurrence GA-ONFH remain unclear. We employed vivo mouse models clinical samples from patients to investigate interactions BMSCs. Immunofluorescence staining was used assess colocalization Flow cytometry transcriptomic analysis were performed evaluate impact exosomes derived normal (n-M1) (GC-M1) on BMSC differentiation. Additionally, miR-1a-3p expression altered vitro its role adipogenic In vivo, observed, an macrophage numbers decrease repair capabilities further confirmed both models. Both n-M1 GC-M1 identified as contributors inhibition osteogenic differentiation certain extent via exosome secretion. More importantly, exhibited heightened capacity regulate BMSCs, which mediated by miR-1a-3p. vitro, promoted targeting Cebpz played important onset progression GA-ONFH. demonstrated that disrupt balance contributing pathogenesis Inhibiting expression, significantly mitigates preferential thus slowing These findings provide new insights into highlight potential therapeutic targets for intervention.

Язык: Английский

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Osteoimmune Interactions and Therapeutic Potential of Macrophage-Derived Small Extracellular Vesicles in Bone-Related Diseases

International Journal of Nanomedicine, Год журнала: 2023, Номер Volume 18, С. 2163 - 2180

Опубликована: Апрель 1, 2023

Abstract: Due to the aging of global population, burden bone-related diseases has increased sharply. Macrophage, as indispensable components both innate immune responses and adaptive immunity, plays a considerable role in maintaining bone homeostasis promoting establishment. Small extracellular vesicles (sEVs) have attracted increasing attention because they participate cell cross-talk pathological environments can serve drug delivery systems. In recent years, an number studies expanded our knowledge about effects macrophage-derived sEVs (M-sEVs) via different forms polarization their biological functions. this review, we comprehensively describe on application mechanisms M-sEVs various delivery, which may provide new perspectives for treating diagnosing human disorders, especially osteoporosis, arthritis, osteolysis, defects. Keywords: small vesicles, macrophage, crosstalk,

Язык: Английский

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Aucubin suppresses TLR4/NF‐κB signalling to shift macrophages toward M2 phenotype in glucocorticoid‐associated osteonecrosis of the femoral head

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(15)

Опубликована: Авг. 1, 2024

In this study, we investigated whether the ability of aucubin to mitigate pathology GONFH involves suppression TLR4/NF-κB signalling and promotion macrophage polarization an M2 phenotype. necrotic bone tissues from patients, compared levels pro-inflammatory M1 macrophages anti-inflammatory as well signalling. a rat model GONFH, examined effects on these parameters. We further explored its mechanism action in cell culture macrophages. Necrotic patients contained significantly increased M1/M2 ratio, higher TLR4, MYD88 NF-κB p65 than with hip osteoarthritis. Treating rats mitigated necrosis demineralization destruction trabecular marrow dose-dependent manner, based micro-computed tomography. These therapeutic were associated decrease overall number macrophages, proportion increase downregulation p65. vivo confirmed by treating cultures macrophage-like cells aucubin. Aucubin mitigates suppressing shift pro-

Язык: Английский

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[Role and mechanism of macrophage-mediated osteoimmune in osteonecrosis of the femoral head].

PubMed, Год журнала: 2024, Номер 38(1), С. 119 - 124

Опубликована: Янв. 15, 2024

To summarize the research progress on role of macrophage-mediated osteoimmune in osteonecrosis femoral head (ONFH) and its mechanisms.

Язык: Английский

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Boosting mRNA‐Engineered Monocytes via Prodrug‐Like Microspheres for Bone Microenvironment Multi‐Phase Remodeling

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 6, 2024

Abstract Monocytes, as progenitors of macrophages and osteoclasts, play critical roles in various stages bone repair, necessitating phase‐specific regulatory mechanisms. Here, icariin (ICA) prodrug‐like microspheres (ICA@GM) are developed, lipid nanoparticle (LNP) transfection boosters, to construct mRNA‐engineered monocytes for remodeling the microenvironment across multiple stages, including acute inflammatory repair phases. Initially, ICA@GM is prepared from ICA‐conjugated gelatin methacryloyl via a microfluidics system. Then, monocyte‐targeting IL‐4 mRNA‐LNPs then integrated into injectable (mRNA‐ICA@GM) electrostatic hydrogen bond interactions. After bone‐defect injection, LNPs controlled released mRNA‐ICA@GM within 3 days, rapidly transfecting monocyte mRNA‐engineering, which effectively suppressed responses polarization programming paracrine signaling. Afterwards, ICA sustainably well cleavable boronate esters cooperatively boosting inhibit coenocytic fusion osteoclastic function. Both vitro vivo data indicated that can not only reverse environment but also suppress monocyte‐derived osteoclast formation accelerate repair. In summary, combined achieve long‐lasting multi‐stage regulation, offering promising strategy.

Язык: Английский

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