Camellia sinensis methanolic leaves extract: Phytochemical analysis and anticancer activity against human liver cancer cells DOI Creative Commons
Demiana H. Hanna,

Ahlam K. Al-Atmani,

Ayshah Aysh ALrashidi

и другие.

PLoS ONE, Год журнала: 2024, Номер 19(11), С. e0309795 - e0309795

Опубликована: Ноя. 14, 2024

Background The study’s primary goal is to ascertain whether there a relationship between the processed green tea methanolic extract’s (GTME) phytochemical components and its potential effectiveness against human liver cancer cells. GTME’s composition was identified using gas chromatography-mass spectrometry, capacity lower cellular proliferation cause apoptosis in HepG2 cancerous cell lines checked. Results findings of chromatogram showed that GTME included bioactive antioxidants anticancer substances. Additionally, utilizing MTT, comet assay, acridine revealed selective cytotoxic impact with significant IC 50 value (27.3 µg/ml) on cells without any harmful effects WI-38 healthy Also, compared untreated cells, extract-treated had an upsurge proportion have undergone displayed nucleus, which sign DNA damage. In addition, treated stop G1 phase sub-G1 apoptotic (37.32%) flow cytometry analysis. Furthermore, reactive oxygen species were shown be responsible for apoptosis, tested extract significantly reduced their levels Lastly, changed protein expression linked cycle arrest apoptosis. Conclusion These provided information about processes through inhibited growth HepG2. Therefore, it has as effective natural therapy treatment cancer. However, validate these findings, animal models must used vivo studies.

Язык: Английский

Camellia sinensis methanolic leaves extract: Phytochemical analysis and anticancer activity against human liver cancer cells DOI Creative Commons
Demiana H. Hanna,

Ahlam K. Al-Atmani,

Ayshah Aysh ALrashidi

и другие.

PLoS ONE, Год журнала: 2024, Номер 19(11), С. e0309795 - e0309795

Опубликована: Ноя. 14, 2024

Background The study’s primary goal is to ascertain whether there a relationship between the processed green tea methanolic extract’s (GTME) phytochemical components and its potential effectiveness against human liver cancer cells. GTME’s composition was identified using gas chromatography-mass spectrometry, capacity lower cellular proliferation cause apoptosis in HepG2 cancerous cell lines checked. Results findings of chromatogram showed that GTME included bioactive antioxidants anticancer substances. Additionally, utilizing MTT, comet assay, acridine revealed selective cytotoxic impact with significant IC 50 value (27.3 µg/ml) on cells without any harmful effects WI-38 healthy Also, compared untreated cells, extract-treated had an upsurge proportion have undergone displayed nucleus, which sign DNA damage. In addition, treated stop G1 phase sub-G1 apoptotic (37.32%) flow cytometry analysis. Furthermore, reactive oxygen species were shown be responsible for apoptosis, tested extract significantly reduced their levels Lastly, changed protein expression linked cycle arrest apoptosis. Conclusion These provided information about processes through inhibited growth HepG2. Therefore, it has as effective natural therapy treatment cancer. However, validate these findings, animal models must used vivo studies.

Язык: Английский

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