Advanced Healthcare Materials,
Год журнала:
2018,
Номер
7(14)
Опубликована: Июнь 3, 2018
Adding
value
to
the
intrinsic
properties
of
quantum
dots
(QDs),
a
strategy
conjugate
dyes
on
surface
QDs
offers
new
opportunities,
since
coupling
between
QD
and
can
be
designed
allow
Förster
resonance
energy
transfer
(FRET)
and/or
electron
(eT).
These
processes
are
accompanied
by
change
dye
fluorescence
subsequent
photochemical
reactions
(e.g.,
generation
1
O2
).
Based
signals
interaction
with
biomolecules,
QD-dye
conjugates
exploited
as
biosensors
for
detection
pH,
,
nicotinamide
adenine
dinucleotide
(phosphate),
ions,
proteases,
glutathione,
microRNA.
also
modulated
irradiation
external
light;
this
concept
is
demonstrated
super-resolution
imaging
photoactivatable
or
photoswitchable
probes.
When
conjugated
photosensitizing
dyes,
generate
in
repetitive
manner
better
cancer
treatment,
available
approaches
using
two-photon
excitation
bioluminescence
mechanisms
deep
tissue
imaging.
Here,
recent
advances
conjugates,
where
FRET
eT
produces
readouts
reactions,
reviewed.
Various
systems
their
biosensing/imaging
photodynamic
therapeutics
summarized.
Physiological Reviews,
Год журнала:
2019,
Номер
99(4), С. 2015 - 2113
Опубликована: Сен. 11, 2019
Na+/H+
exchangers
play
pivotal
roles
in
the
control
of
cell
and
tissue
pH
by
mediating
electroneutral
exchange
Na+
H+
across
cellular
membranes.
They
belong
to
an
ancient
family
highly
evolutionarily
conserved
proteins,
they
essential
physiological
all
phyla.
In
this
review,
we
focus
on
mammalian
(NHEs),
solute
carrier
(SLC)
9
family.
This
transporters
constitutes
three
branches:
SLC9A,
-B,
-C.
Within
these,
each
isoform
exhibits
distinct
expression
profiles,
regulation,
roles.
Some
these
are
studied,
with
hundreds
original
articles,
some
still
only
rudimentarily
understood.
present
discuss
pioneering
work
as
well
current
state-of-the-art
research
NHEs.
We
aim
provide
reader
a
comprehensive
view
core
knowledge
recent
insights
into
member,
from
gene
organization
over
protein
structure
regulation
pathophysiological
Particular
attention
is
given
integrated
physiology
NHEs
main
organ
systems.
several
novel
analyses
useful
overviews,
pinpoint
remaining
enigmas,
which
hope
will
inspire
versatile
proteins.
Journal of the American Society of Nephrology,
Год журнала:
2020,
Номер
31(3), С. 469 - 482
Опубликована: Янв. 27, 2020
Retrospective
analyses
and
single-center
prospective
studies
identify
chronic
metabolic
acidosis
as
an
independent
modifiable
risk
factor
for
progression
of
CKD.
In
patients
with
CKD,
untreated
often
leads
to
accelerated
reduction
in
GFR.
Mechanisms
responsible
this
include
adaptive
responses
that
increase
acid
excretion
but
lead
a
decline
kidney
function.
Metabolic
CKD
stimulates
production
intrakidney
paracrine
hormones
including
angiotensin
II,
aldosterone,
endothelin-1
(ET-1)
mediate
the
immediate
benefit
increased
excretion,
their
upregulation
promotes
inflammation
fibrosis.
Chronic
also
ammoniagenesis
increases
ammonia-induced
complement
activation
deposition
C3
C5b-9
can
cause
tubule-interstitial
damage,
further
worsening
disease
progression.
These
effects,
along
accumulation
tissue,
combine
accelerate
disease.
Treatment
attenuates
these
responses;
reduces
levels
ET-1;
ammoniagenesis;
diminishes
fibrosis
may
slowing
Kidney
Precision
Medicine
Project
(KPMP)
is
building
a
spatially
specified
human
kidney
tissue
atlas
in
health
and
disease
with
single-cell
resolution.
Here,
we
describe
the
construction
of
an
integrated
reference
map
cells,
pathways,
genes
using
unaffected
regions
nephrectomy
tissues
undiseased
biopsies
from
56
adult
subjects.
We
use
single-cell/nucleus
transcriptomics,
subsegmental
laser
microdissection
transcriptomics
proteomics,
near-single-cell
3D
CODEX
imaging,
spatial
metabolomics
to
hierarchically
identify
genes,
cells.
Integrated
data
these
different
technologies
coherently
cell
types/subtypes
within
nephron
segments
interstitium.
These
profiles
cell-level
functional
organization
following
its
physiological
functions
link
subtypes
proteins,
metabolites,
pathways.
They
further
show
that
messenger
RNA
levels
along
are
congruent
activity.
This
provides
framework
for
classification
when
multiple
molecular
mechanisms
underlie
convergent
clinical
phenotypes.
Advances in Nutrition,
Год журнала:
2019,
Номер
10, С. S367 - S379
Опубликована: Авг. 20, 2019
Kidney
disease
affects
almost
15%
of
the
US
population,
and
prevalence
is
anticipated
to
grow
as
population
ages
obesity
epidemic
continues
due
Western
dietary
practices.
The
densely
caloric
diet,
characterized
by
high
animal
protein
low
fruit
vegetable
content,
has
fueled
growth
chronic
diseases,
including
kidney
disease.
glomerulus
or
filtering
unit
very
susceptible
barotrauma,
diets
in
impede
glomerulus'
ability
protect
itself
from
hemodynamic
injury.
High
intake
combined
with
fruits
vegetables
also
leads
a
net
endogenous
acid
production
requiring
augmentation
ammonium
excretion
order
prevent
acidosis.
This
higher
workload
maintain
normal
serum
bicarbonate
level
may
further
exacerbate
progression.
article
reviews
potential
mechanisms
whereby
several
key
characteristics
typical
diet
impact
incidence
Reducing
egg
yolk
increasing
fiber
delay
end-stage
renal
disease,
but
few
clinical
trials
have
examined
vegetarian
for
management
More
research
needed
determine
optimal
patterns
prevention
its
Nature Communications,
Год журнала:
2019,
Номер
10(1)
Опубликована: Дек. 13, 2019
Abstract
Although
kidney
parenchymal
tissue
can
be
generated
in
vitro,
reconstructing
the
complex
vasculature
of
remains
a
daunting
task.
The
molecular
pathways
that
specify
and
sustain
functional,
phenotypic
structural
heterogeneity
are
unknown.
Here,
we
employ
high-throughput
bulk
single-cell
RNA
sequencing
non-lymphatic
endothelial
cells
(ECs)
to
identify
dictate
vascular
zonation
from
embryos
adulthood.
We
show
manifests
vascular-specific
signatures
expressing
defined
transcription
factors,
ion
channels,
solute
transporters,
angiocrine
factors
choreographing
functions.
Notably,
ontology
glomerulus
coincides
with
induction
unique
including
Tbx3,
Gata5,
Prdm1
,
Pbx1
.
Deletion
Tbx3
ECs
results
glomerular
hypoplasia,
microaneurysms
regressed
fenestrations
leading
fibrosis
subsets
glomeruli.
Deciphering
determinants
lays
foundation
for
rebuilding
nephrons
uncovering
pathogenesis
disorders.
Molecular Pharmaceutics,
Год журнала:
2017,
Номер
14(8), С. 2475 - 2486
Опубликована: Май 2, 2017
Stimuli
responsive
polymers
have
been
extensively
studied
as
nanocarriers
for
drug
delivery
systems
(DDSs),
especially
those
based
on
supramolecular
interactions.
Cyclodextrin
(CD)
is
one
kind
of
widely
applied
host
molecule,
and
the
host-guest
interactions
between
CD
different
counterparts
can
respond
to
stimuli
thus
be
linkers
polymeric
DDSs.
In
this
review,
ferrocene,
azobenzene,
benzimidazole
DDSs
are
summarized,
with
redox,
light,
pH
sensitivity,
respectively.
The
mechanisms
ability
linkers,
application
them
construction
polymer
structures,
controlled
release
behaviors
focused.
addition,
outlook
challenge
these
discussed.
Advanced Functional Materials,
Год журнала:
2019,
Номер
29(31)
Опубликована: Май 29, 2019
Abstract
Acid‐base
homeostasis
is
crucial
for
normal
physiology,
metabolism,
and
functions
of
living
organisms.
Thus,
the
development
effective
techniques
to
monitor
it
either
in
vitro
or
vivo
great
demand.
Herein,
a
series
multifunctional
oxazine‐containing
polyheterocycles
with
aggregation‐induced
emission
characteristics
are
situ
generated
by
metal‐free
one‐pot
A
3
polymerizations
terminal
diynes,
dialdehydes,
ureas.
Most
polymers
synthesized
high
molecular
weights
(
M
w
up
49
900)
nearly
quantitative
yields
display
intense
solid‐state
remarkable
fluorescence
response
protonation
deprotonation.
Based
on
unique
photophysical
properties,
fast
responsive
reversible
fluorescent
sensor
ammonia
detection
limit
960
ppb
achieved
applied
detecting
biogenic
amines
seafood
spoilage.
Besides,
polymeric
nanoparticles
show
excellent
lysosome‐targeting
specificity
cell
imaging.
The
ratiometric
pH
sensing
behavior
broad
acid‐base
window
from
1
9,
which
shed
light
visualizing
physiologic
gastrointestinal
tract.
Taking
freshwater
Cladocera
Moina
macrocopa
as
model
organism,
mapping
its
intestinal
shows
an
increased
gradient
approximately
4.2
7.8
along
foregut,
midgut,
hindgut.
