Supramolecular fluorescent sensors: An historical overview and update

Coordination Chemistry Reviews, Journal Year: 2020, Volume and Issue: 427, P. 213560 - 213560

Published: Sept. 15, 2020

Language: Английский

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The SLC9A-C Mammalian Na⁺/H⁺Exchanger Family: Molecules, Mechanisms, and Physiology

Physiological Reviews, Journal Year: 2019, Volume and Issue: 99(4), P. 2015 - 2113

Published: Sept. 11, 2019

Na+/H+ exchangers play pivotal roles in the control of cell and tissue pH by mediating electroneutral exchange Na+ H+ across cellular membranes. They belong to an ancient family highly evolutionarily conserved proteins, they essential physiological all phyla. In this review, we focus on mammalian (NHEs), solute carrier (SLC) 9 family. This transporters constitutes three branches: SLC9A, -B, -C. Within these, each isoform exhibits distinct expression profiles, regulation, roles. Some these are studied, with hundreds original articles, some still only rudimentarily understood. present discuss pioneering work as well current state-of-the-art research NHEs. We aim provide reader a comprehensive view core knowledge recent insights into member, from gene organization over protein structure regulation pathophysiological Particular attention is given integrated physiology NHEs main organ systems. several novel analyses useful overviews, pinpoint remaining enigmas, which hope will inspire versatile proteins.

Language: Английский

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Mechanisms of Metabolic Acidosis–Induced Kidney Injury in Chronic Kidney Disease

Journal of the American Society of Nephrology, Journal Year: 2020, Volume and Issue: 31(3), P. 469 - 482

Published: Jan. 27, 2020

Retrospective analyses and single-center prospective studies identify chronic metabolic acidosis as an independent modifiable risk factor for progression of CKD. In patients with CKD, untreated often leads to accelerated reduction in GFR. Mechanisms responsible this include adaptive responses that increase acid excretion but lead a decline kidney function. Metabolic CKD stimulates production intrakidney paracrine hormones including angiotensin II, aldosterone, endothelin-1 (ET-1) mediate the immediate benefit increased excretion, their upregulation promotes inflammation fibrosis. Chronic also ammoniagenesis increases ammonia-induced complement activation deposition C3 C5b-9 can cause tubule-interstitial damage, further worsening disease progression. These effects, along accumulation tissue, combine accelerate disease. Treatment attenuates these responses; reduces levels ET-1; ammoniagenesis; diminishes fibrosis may slowing

Language: Английский

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A reference tissue atlas for the human kidney

Science Advances, Journal Year: 2022, Volume and Issue: 8(23)

Published: June 8, 2022

Kidney Precision Medicine Project (KPMP) is building a spatially specified human kidney tissue atlas in health and disease with single-cell resolution. Here, we describe the construction of an integrated reference map cells, pathways, genes using unaffected regions nephrectomy tissues undiseased biopsies from 56 adult subjects. We use single-cell/nucleus transcriptomics, subsegmental laser microdissection transcriptomics proteomics, near-single-cell 3D CODEX imaging, spatial metabolomics to hierarchically identify genes, cells. Integrated data these different technologies coherently cell types/subtypes within nephron segments interstitium. These profiles cell-level functional organization following its physiological functions link subtypes proteins, metabolites, pathways. They further show that messenger RNA levels along are congruent activity. This provides framework for classification when multiple molecular mechanisms underlie convergent clinical phenotypes.

Language: Английский

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Severely polarized extracellular acidity around tumour cells

Nature Biomedical Engineering, Journal Year: 2024, Volume and Issue: 8(6), P. 787 - 799

Published: March 4, 2024

Language: Английский

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Diet and Chronic Kidney Disease

Advances in Nutrition, Journal Year: 2019, Volume and Issue: 10, P. S367 - S379

Published: Aug. 20, 2019

Kidney disease affects almost 15% of the US population, and prevalence is anticipated to grow as population ages obesity epidemic continues due Western dietary practices. The densely caloric diet, characterized by high animal protein low fruit vegetable content, has fueled growth chronic diseases, including kidney disease. glomerulus or filtering unit very susceptible barotrauma, diets in impede glomerulus' ability protect itself from hemodynamic injury. High intake combined with fruits vegetables also leads a net endogenous acid production requiring augmentation ammonium excretion order prevent acidosis. This higher workload maintain normal serum bicarbonate level may further exacerbate progression. article reviews potential mechanisms whereby several key characteristics typical diet impact incidence Reducing egg yolk increasing fiber delay end-stage renal disease, but few clinical trials have examined vegetarian for management More research needed determine optimal patterns prevention its

Language: Английский

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Molecular determinants of nephron vascular specialization in the kidney

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: Dec. 13, 2019

Abstract Although kidney parenchymal tissue can be generated in vitro, reconstructing the complex vasculature of remains a daunting task. The molecular pathways that specify and sustain functional, phenotypic structural heterogeneity are unknown. Here, we employ high-throughput bulk single-cell RNA sequencing non-lymphatic endothelial cells (ECs) to identify dictate vascular zonation from embryos adulthood. We show manifests vascular-specific signatures expressing defined transcription factors, ion channels, solute transporters, angiocrine factors choreographing functions. Notably, ontology glomerulus coincides with induction unique including Tbx3, Gata5, Prdm1 , Pbx1 . Deletion Tbx3 ECs results glomerular hypoplasia, microaneurysms regressed fenestrations leading fibrosis subsets glomeruli. Deciphering determinants lays foundation for rebuilding nephrons uncovering pathogenesis disorders.

Language: Английский

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Polymeric Nanocarriers Based on Cyclodextrins for Drug Delivery: Host–Guest Interaction as Stimuli Responsive Linker

Molecular Pharmaceutics, Journal Year: 2017, Volume and Issue: 14(8), P. 2475 - 2486

Published: May 2, 2017

Stimuli responsive polymers have been extensively studied as nanocarriers for drug delivery systems (DDSs), especially those based on supramolecular interactions. Cyclodextrin (CD) is one kind of widely applied host molecule, and the host-guest interactions between CD different counterparts can respond to stimuli thus be linkers polymeric DDSs. In this review, ferrocene, azobenzene, benzimidazole DDSs are summarized, with redox, light, pH sensitivity, respectively. The mechanisms ability linkers, application them construction polymer structures, controlled release behaviors focused. addition, outlook challenge these discussed.

Language: Английский

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Visualization of Biogenic Amines and In Vivo Ratiometric Mapping of Intestinal pH by AIE‐Active Polyheterocycles Synthesized by Metal‐Free Multicomponent Polymerizations

Advanced Functional Materials, Journal Year: 2019, Volume and Issue: 29(31)

Published: May 29, 2019

Abstract Acid‐base homeostasis is crucial for normal physiology, metabolism, and functions of living organisms. Thus, the development effective techniques to monitor it either in vitro or vivo great demand. Herein, a series multifunctional oxazine‐containing polyheterocycles with aggregation‐induced emission characteristics are situ generated by metal‐free one‐pot A 3 polymerizations terminal diynes, dialdehydes, ureas. Most polymers synthesized high molecular weights ( M w up 49 900) nearly quantitative yields display intense solid‐state remarkable fluorescence response protonation deprotonation. Based on unique photophysical properties, fast responsive reversible fluorescent sensor ammonia detection limit 960 ppb achieved applied detecting biogenic amines seafood spoilage. Besides, polymeric nanoparticles show excellent lysosome‐targeting specificity cell imaging. The ratiometric pH sensing behavior broad acid‐base window from 1 9, which shed light visualizing physiologic gastrointestinal tract. Taking freshwater Cladocera Moina macrocopa as model organism, mapping its intestinal shows an increased gradient approximately 4.2 7.8 along foregut, midgut, hindgut.

Language: Английский

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Acid-base physiology and CO2 homeostasis: Regulation and compensation in response to elevated environmental CO2

Fish physiology, Journal Year: 2019, Volume and Issue: unknown, P. 69 - 132

Published: Jan. 1, 2019

Language: Английский

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