Exploring Endogenous Tryptamines: Overlooked Agents Against Fibrosis in Chronic Disease? A Narrative Review DOI Creative Commons
Hunter Korsmo

Livers, Год журнала: 2024, Номер 4(4), С. 615 - 637

Опубликована: Ноя. 28, 2024

Long regarded as illicit substances with no clinical value, N-dimethylated tryptamines—such N,N-dimethyltryptamine, 5-methoxy-N,N-dimethyltryptamine, and bufotenine—have been found to produce naturally in a wide variety of species, including humans. Known for their psychoactive effects through serotonin receptors (5-HTRs), tryptamines are currently being reinvestigated clinically long-term benefits mental disorders. Endogenous tryptamine is methylated by indolethylamine-N-methyltransferase (INMT), which can then serve an agonist pro-survival pathways, such sigma non-opioid intracellular receptor 1 (SIGMAR1) signaling. Fibrogenic diseases, like metabolic-associated fatty liver disease (MAFLD), steatohepatitis (MASH), chronic kidney (CKD) have shown changes INMT SIGMAR1 activity the progression pathogenesis. At cellular level, endothelial cells fibroblasts express various tissues; however, little known about injury fibrosis. In this review, I will give overview biochemistry, molecular biology, current evidence INMT’s role hepatic fibrogenesis. also discuss pre-clinical findings N-methylated highlight new upcoming therapeutic strategies that may be adapted mitigating fibrogenic diseases. Finally, mention recent mutualistic gut bacteria influencing endogenous signaling metabolism.

Язык: Английский

Targeting regulated cell death: Apoptosis, necroptosis, pyroptosis, ferroptosis, and cuproptosis in anticancer immunity DOI
Ziyu Guo,

Yihuang Liu,

Danyao Chen

и другие.

Journal of Translational Internal Medicine, Год журнала: 2025, Номер 13(1), С. 10 - 32

Опубликована: Фев. 1, 2025

In the evolving landscape of cancer treatment, strategic manipulation regulated cell death (RCD) pathways has emerged as a crucial component effective anti-tumor immunity. Evidence suggests that tumor cells undergoing RCD can modify immunogenicity microenvironment (TME), potentially enhancing its ability to suppress progression and metastasis. this review, we first explore mechanisms apoptosis, necroptosis, pyroptosis, ferroptosis, cuproptosis, along with crosstalk between these modalities. We then discuss how processes activate antigen-presenting cells, facilitate cross-priming CD8+ T trigger immune responses, highlighting complex effects novel forms on TME biology. Furthermore, summarize potential drugs nanoparticles induce or inhibit emerging their therapeutic roles in treatment. Finally, put forward existing challenges future prospects for targeting anti-cancer Overall, review enhances our understanding molecular biological impacts RCD-based therapies, providing new perspectives strategies

Язык: Английский

Процитировано

1

Ferroptosis: A Targetable Vulnerability for Melanoma Treatment DOI Creative Commons

Meng Yu,

Qian Zhou,

Yating Dian

и другие.

Journal of Investigative Dermatology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Melanoma is a devastating form of skin cancer characterized by high mutational burden, limited treatment success, and dismal prognosis. Although immunotherapy targeted therapies have significantly revolutionized melanoma treatment, the majority patients fail to achieve durable responses, highlighting urgent need for novel therapeutic strategies. Ferroptosis, an iron-dependent regulated cell death driven overwhelming accumulation lipid peroxides, has emerged as promising approach in preclinical models. A deeper understanding ferroptosis landscape based on its biology characteristics, including phenotypic plasticity, metabolic state, genomic alterations, epigenetic changes, well complex role mechanisms immune cells could provide foundation developing effective treatments. In this review, we outline molecular ferroptosis, decipher regulation, reveal potential melanoma, discuss pressing questions that should guide future investigations into melanoma.

Язык: Английский

Процитировано

0

Single-cell multi-omics analysis identifies SPP1+ macrophages as key drivers of ferroptosis-mediated fibrosis in ligamentum flavum hypertrophy DOI Creative Commons

Chengshuo Fei,

Yanlin Chen,

Ruiqian Tan

и другие.

Biomarker Research, Год журнала: 2025, Номер 13(1)

Опубликована: Фев. 25, 2025

Abstract Background Ligamentum flavum hypertrophy (LFH) is a primary contributor to lumbar spinal stenosis. However, thorough understanding of the cellular and molecular mechanisms driving LFH fibrotic progression remains incomplete. Methods Single-cell RNA sequencing (scRNA-seq) was performed construct single-cell map human ligamentum (LF) samples. An integrated multi-omics approach, encompassing scRNA-seq, bulk (bulk RNA-seq), Mendelian randomization (MR), applied conduct comprehensive functional analysis. Clinical tissue specimens animal models were employed further confirm findings. Results ScRNA-seq provided level view microenvironment in LF, revealing significantly increased proportions fibroblasts, myofibroblasts, macrophages LFH. Using transmission electron microscopy, gene set scoring, MR analysis, ferroptosis identified as critical risk factor pathway within Subcluster analysis fibroblasts revealed heterogeneity among distinct subpopulations, highlighting characteristics metabolic dynamics fibroblast with high score (High Ferro-score FB). The quantification expression at that along fibrosis specimens, finding validated both mice sections. Consistently, RNA-seq confirmed underscoring strong correlation between these cell types through Spearman Notably, subcluster mononuclear phagocytes specific subset SPP1 + (SPP1 Mac) enriched LFH, which exhibited activation ferroptosis-related pathways. Cell-cell communication highlighted Mac strongest outgoing incoming interactions microenvironment. Ligand-receptor SPP1-CD44 axis could serve key mediator regulating activity High FB. Multiplex immunofluorescence substantial Collagen I deposition reduced Ferritin Light Chain regions co-localization specimens. Conclusions Our findings indicated may contribute by FB axis. This study enhances our underlying progression, potentially improving early diagnostic strategies identifying new therapeutic targets.

Язык: Английский

Процитировано

0

Dysregulated metabolic pathways of pulmonary fibrosis and the lipids associated with the effects of nintedanib therapy DOI Creative Commons
Yasuo Shimizu, Yasuhiro Horibata,

Mariko Domae

и другие.

Respiratory Research, Год журнала: 2025, Номер 26(1)

Опубликована: Апрель 28, 2025

Pulmonary fibrosis (PF) is a disease with poor prognosis, and its pathogenesis not fully understood. Identifying dysregulation of lipid metabolism in PF may provide insight promote the development novel therapies. The present study was designed to clarify dysregulated pathways identify lipids correlated treatment response. This research comprised two prospective cohort studies. Study 1 aimed metabolic peripheral blood patients, compared healthy control (HC) subjects. 2 associated decline % forced vital capacity (%FVC) survival patients treated anti-fibrotic drug, nintedanib. As preliminary ancillary experiment, we attempted endothelial cells fibroblasts. In 1, 38 were identified that differed between (n = 66) HC 63) groups. Compared subjects, phosphatidylcholine (PC) 36:5 most up-regulated lysophosphatidylcholine (LPC) 18:0 down-regulated patients. Glycerophospholipid enriched pathway. Plasmenyl phosphatidylethanolamine (pPE) plasmanyl (pPC) determined be endothelial-related lipids, (PE) fibroblast-related PF. 2, 10 absolute %FVC < 2.5% group (6 M responders, n 14) > non-responders, 6) after 6 nintedanib therapy, 5% (12 15) 5) 12 therapy. Four consistently detected at M, among them, higher levels pPE 18:0p/22:6 showed poorer prognosis for 24 (p 0.05, HR 6.547, 95% CI 1.471-29.13). Under species progressive fibrosis, considered an lipid. Lipidomic profiling revealed distinct strongly responses Targeting or catabolic enzymes involved has potential ameliorate Registry UMIN, analysis on plasma idiopathic pulmonary Trial registry number, UMIN000020872. Registered 3 February 2016, https://center6.umin.ac.jp/cgiopenbin/ctr/index.cgi .

Язык: Английский

Процитировано

0

Mechanistic role of environmental toxicants in inducing cellular ferroptosis and its associated diseases DOI
Hong Chen,

Bingchun Liu,

Peixin Xu

и другие.

Ecotoxicology and Environmental Safety, Год журнала: 2025, Номер 298, С. 118269 - 118269

Опубликована: Май 10, 2025

Язык: Английский

Процитировано

0

Asiaticoside enhances the effect of propofol on the invasion, ferroptosis and immune escape of bladder cancer DOI

Ming Jin,

Kunlun He,

Shuqing Zhen

и другие.

Drug Development Research, Год журнала: 2024, Номер 85(6)

Опубликована: Авг. 19, 2024

Bladder cancer is a highly prevalent malignancy. Asiaticoside (AC), triterpenoid derivative, exhibits antitumor effect on different tumors. This study aimed to explore the role and mechanism of AC bladder cancer. J82 T24 cells were treated with and/or propofol, nude mice subcutaneously administrated cells. The propofol explored by cell counting kit-8, transwell, flow cytometry, enzyme-linked immunosorbent assay, immunohistochemistry western blot assays both in vitro vivo. Cell viability was inhibited IC50 value 2.43 μM 2.16 μM, 42.51 48.37 respectively. or alone decreased proliferation, invasion, immune escape increased ferroptosis, as well downregulating level PI3K/AKT pathway animal experiments. above-mentioned indicators further enhanced co-treatment Taken together, promoted ameliorative involved pathway.

Язык: Английский

Процитировано

2

Peritoneal fibrosis: from pathophysiological mechanism to medicine DOI Creative Commons

Yingxi Kang,

Yuan Liu, Ping Fu

и другие.

Frontiers in Physiology, Год журнала: 2024, Номер 15

Опубликована: Сен. 4, 2024

Peritoneal dialysis (PD) is currently one of the effective methods for treating end-stage renal disease (ESRD). However, long-term exposure to high concentration glucose in peritoneal environment could lead fibrosis (PF), impaired filtration function, decreased efficiency, and even withdrawal from patients. Considerable evidence suggests that after related crucial factors such as mesothelial-to-mesenchymal transition (MMT), inflammatory response, angiogenesis, etc. In our review, we summarize pathophysiological mechanisms further illustrate future strategies against PF.

Язык: Английский

Процитировано

1

Exploring Endogenous Tryptamines: Overlooked Agents Against Fibrosis in Chronic Disease? A Narrative Review DOI Creative Commons
Hunter Korsmo

Livers, Год журнала: 2024, Номер 4(4), С. 615 - 637

Опубликована: Ноя. 28, 2024

Long regarded as illicit substances with no clinical value, N-dimethylated tryptamines—such N,N-dimethyltryptamine, 5-methoxy-N,N-dimethyltryptamine, and bufotenine—have been found to produce naturally in a wide variety of species, including humans. Known for their psychoactive effects through serotonin receptors (5-HTRs), tryptamines are currently being reinvestigated clinically long-term benefits mental disorders. Endogenous tryptamine is methylated by indolethylamine-N-methyltransferase (INMT), which can then serve an agonist pro-survival pathways, such sigma non-opioid intracellular receptor 1 (SIGMAR1) signaling. Fibrogenic diseases, like metabolic-associated fatty liver disease (MAFLD), steatohepatitis (MASH), chronic kidney (CKD) have shown changes INMT SIGMAR1 activity the progression pathogenesis. At cellular level, endothelial cells fibroblasts express various tissues; however, little known about injury fibrosis. In this review, I will give overview biochemistry, molecular biology, current evidence INMT’s role hepatic fibrogenesis. also discuss pre-clinical findings N-methylated highlight new upcoming therapeutic strategies that may be adapted mitigating fibrogenic diseases. Finally, mention recent mutualistic gut bacteria influencing endogenous signaling metabolism.

Язык: Английский

Процитировано

0