T cell interaction partners of DHHC20 DOI Open Access

Deana Haxhiraj,

Benno Kuropka, Eva Brencher

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 15, 2024

Abstract Reversible palmitoylation of proteins at cysteine residues represents a post-translational modification that can alter the cellular localization proteins, change their distribution within lipid membranes or modulate conformation and molecular interaction patterns. DHHC enzymes catalyze protein palmitoylation, while thioesterases hydrolases rapidly remove acyl-chain. In human T cells, have been shown to modify are involved in major signaling pathways such as Ca 2+ -signaling kinase-dependent activation transcription factors for cytokines. For DHHC20, role Orai1 -channel has demonstrated, but otherwise its cell partners largely unknown. Here, we show recombinantly expressed DHHC20 robustly interacts with 28 from Jurkat by affinity enrichment combined mass spectrometric analysis. We find robust between β-subunit trimeric G Gαsβ1γ2, typically palmitoylated Ga subunit is not identified. Cross-linking spectrometry purified Gαsβ1γ2 then confirms direct β1γ2 domains enzyme, rigid docking offers structural poses agreement observed intermolecular cross-linking constraints. Thus, suggest model where subunits serve stable partner presumably acting landing platform Gα subsequently enzyme.

Язык: Английский

Mechanisms and functions of protein S-acylation DOI
Francisco S. Mesquita, Laurence Abrami, Maurine E. Linder

и другие.

Nature Reviews Molecular Cell Biology, Год журнала: 2024, Номер 25(6), С. 488 - 509

Опубликована: Фев. 14, 2024

Язык: Английский

Процитировано

62
TMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress DOI Creative Commons
Marika Kucińska, Juliette Fédry,

Carmela Galli

и другие.

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Июнь 13, 2023

The endoplasmic reticulum (ER) is an organelle of nucleated cells that produces proteins, lipids and oligosaccharides. ER volume activity are increased upon induction unfolded protein responses (UPR) reduced activation ER-phagy programs. A specialized domain the ER, nuclear envelope (NE), protects cell genome with two juxtaposed lipid bilayers, inner outer membranes (INM ONM) separated by perinuclear space (PNS). Here we report expansion mammalian homeostatic perturbations results in TMX4 reductase-driven disassembly LINC complexes connecting INM ONM swelling. physiologic distance between restored, resolution stress, asymmetric autophagy NE, which involves LC3 lipidation machinery, receptor SEC62 direct capture ONM-derived vesicles degradative LAMP1/RAB7-positive endolysosomes a catabolic pathway mechanistically defined as micro-ONM-phagy.

Язык: Английский

Процитировано

29
Sculpting nuclear envelope identity from the endoplasmic reticulum during the cell cycle DOI Creative Commons
Pallavi Deolal, Julia Scholz, Kaike Ren

и другие.

Nucleus, Год журнала: 2024, Номер 15(1)

Опубликована: Янв. 18, 2024

The nuclear envelope (NE) regulates functions, including transcription, nucleocytoplasmic transport, and protein quality control. While the outer membrane of NE is directly continuous with endoplasmic reticulum (ER), has an overall distinct composition from ER, which crucial for its functions. During open mitosis in higher eukaryotes, disassembles during mitotic entry then reforms as a functional territory at end to reestablish compartmentalization. In this review, we examine known mechanisms by reconstitutes ER ER–NE endomembrane system mitosis. Furthermore, based on recent findings indicating that possesses unique lipid metabolism control those explore maintenance identity homeostasis interphase. We also highlight potential significance junctions between NE.

Язык: Английский

Процитировано

14
Choreography of lamina‐associated domains: structure meets dynamics DOI Open Access

Nicholas S. Alagna,

Tiera I. Thomas,

Katherine L. Wilson

и другие.

FEBS Letters, Год журнала: 2023, Номер 597(22), С. 2806 - 2822

Опубликована: Ноя. 1, 2023

Lamina‐associated domains are large regions of heterochromatin positioned at the nuclear periphery. These have been implicated in gene repression, especially context development. In mammals, LAD organization is dependent on lamins, inner membrane proteins, and chromatin state. addition, readers modifier proteins this organization, potentially serving as molecular tethers that interact with both envelope chromatin. More recent studies focused teasing apart rules govern dynamic how turn, relates to regulation overall 3D genome organization. This review highlights mammalian cells uncovering factors instruct choreography re‐organization, dynamics lamina, including interphase through mitotic exit, when re‐established, well intra‐LAD subdomain variations.

Язык: Английский

Процитировано

16
A lineage-specific protein network at the trypanosome nuclear envelope DOI Creative Commons
Erin R. Butterfield, Samson O. Obado, Simon R. Scutts

и другие.

Nucleus, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 11, 2024

The nuclear envelope (NE) separates translation and transcription is the location of multiple functions, including chromatin organization nucleocytoplasmic transport. molecular basis for many these functions have diverged between eukaryotic lineages. Trypanosoma brucei, a member early branching lineage Discoba, highlights these, distinct lamina kinetochore composition. Here, we describe cohort proteins interacting with both NPC, which term lamina-associated (LAPs). LAPs represent diverse group proteins, two candidate NPC-anchoring pore membrane (POMs) architecture conserved S. cerevisiae H. sapiens, additional peripheral components NPC. While are Kinetoplastid specific, also identified broadly indicating an amalgam divergence conservation within trypanosome NE proteome, highlighting diversity biology across eukaryotes, increasing our understanding NPC evolution.

Язык: Английский

Процитировано

5
Enhancers on the edge — how the nuclear envelope controls gene regulatory elements DOI Creative Commons
Rafal Czapiewski, Eric C. Schirmer

Current Opinion in Genetics & Development, Год журнала: 2024, Номер 87, С. 102234 - 102234

Опубликована: Июль 22, 2024

Precise temporal and sequential control of gene expression during development in response to environmental stimuli requires tight regulation the physical contact between regulatory elements promoters. Current models describing how genome folds 3D space establish these interactions often ignore role most stable structural nuclear feature — envelope. While contributions folding within/between topologically associated domains (TADs) have been extensively described, mechanical from envelope can impact enhancer–promoter both directly indirectly through influencing intra/inter-TAD interactions. Importantly, clearly link this mechanism and, when defective, human disease. Here, we discuss evidence for tissue-specific pairings, potential mechanisms regulation, exciting recent findings that other such as microRNAs long noncoding RNAs are under possible involvement condensates, disruption lead

Язык: Английский

Процитировано

2
PIGB maintains nuclear lamina organization in skeletal muscle of Drosophila DOI Creative Commons
Miki Yamamoto‐Hino,

Masaru Ariura,

Masahito Tanaka

и другие.

The Journal of Cell Biology, Год журнала: 2024, Номер 223(2)

Опубликована: Янв. 23, 2024

The nuclear lamina (NL) plays various roles and participates in integrity, chromatin organization, transcriptional regulation. Lamin proteins, the main components of NL, form a homogeneous meshwork structure under envelope. Lamins are essential, but it is unknown whether their distribution important for function. Here, we found that PIGB, an enzyme involved glycosylphosphatidylinositol (GPI) synthesis, responsible lamin Drosophila. Loss PIGB resulted heterogeneous distributions B-type lamin-binding proteins larval muscles. These phenotypes were rescued by expression lacking GPI synthesis activity. mutant exhibited changes lamina-associated domains large heterochromatic genomic regions reduction stiffness, deformation muscle fibers. results suggest maintains which may be essential mechanical properties.

Язык: Английский

Процитировано

1
Chromatin protein complexes involved in gene repression in lamina-associated domains DOI Creative Commons
Stefano Giustino Manzo, Abdelghani Mazouzi, Christ Leemans

и другие.

The EMBO Journal, Год журнала: 2024, Номер unknown

Опубликована: Сен. 25, 2024

Abstract Lamina-associated domains (LADs) are large chromatin regions that associated with the nuclear lamina (NL) and form a repressive environment for transcription. The molecular players mediate gene repression in LADs currently unknown. Here, we performed FACS-based whole-genome genetic screens human cells using LAD-integrated fluorescent reporters to identify such regulators. Surprisingly, screen identified very few NL proteins, but revealed roles dozens of known Among these negative elongation factor (NELF) complex interacting factors involved RNA polymerase pausing, suggesting regulation transcription is mechanism repress LADs. Furthermore, remodeler BAF activation Mediator can work both as activators repressors LADs, depending on local context possibly by rewiring heterochromatin. Our data indicate fundamental regulators remodeling, rather than interaction play major role within

Язык: Английский

Процитировано

1
Mechanisms of nuclear envelope expansion DOI Creative Commons
Christopher P. Ptak, Saif Rehman, Richard W. Wozniak

и другие.

Current Opinion in Cell Biology, Год журнала: 2024, Номер 91, С. 102425 - 102425

Опубликована: Сен. 8, 2024

Язык: Английский

Процитировано

0
T cell interaction partners of DHHC20 DOI Open Access

Deana Haxhiraj,

Benno Kuropka, Eva Brencher

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 15, 2024

Abstract Reversible palmitoylation of proteins at cysteine residues represents a post-translational modification that can alter the cellular localization proteins, change their distribution within lipid membranes or modulate conformation and molecular interaction patterns. DHHC enzymes catalyze protein palmitoylation, while thioesterases hydrolases rapidly remove acyl-chain. In human T cells, have been shown to modify are involved in major signaling pathways such as Ca 2+ -signaling kinase-dependent activation transcription factors for cytokines. For DHHC20, role Orai1 -channel has demonstrated, but otherwise its cell partners largely unknown. Here, we show recombinantly expressed DHHC20 robustly interacts with 28 from Jurkat by affinity enrichment combined mass spectrometric analysis. We find robust between β-subunit trimeric G Gαsβ1γ2, typically palmitoylated Ga subunit is not identified. Cross-linking spectrometry purified Gαsβ1γ2 then confirms direct β1γ2 domains enzyme, rigid docking offers structural poses agreement observed intermolecular cross-linking constraints. Thus, suggest model where subunits serve stable partner presumably acting landing platform Gα subsequently enzyme.

Язык: Английский

Процитировано

0