Metabolomics and Transcriptomics Reveal the Effects of Different Fermentation Times on Antioxidant Activities of Ophiocordyceps sinensis

Journal of Fungi, Год журнала: 2025, Номер 11(1), С. 51 - 51

Опубликована: Янв. 9, 2025

Ophiocordyceps sinensis is a fungus that cultured through fermentation from wild Chinese cordyceps. While studies have examined its metabolites, the evaluation of antioxidant capacity remains to be conducted. The results O. indicate ferric ion-reducing power (FRAP), (2.74 ± 0.12 μmol Trolox/g), 2,2-diphenyl-1-picrylhydrazyl (DPPH•) free radical scavenging rate (60.21 0.51%), and hydroxyl (91.83 0.68%) reached maximum on day 30. Using LC-MS/MS measure metabolites D24, D30, D36, we found majority differential accumulated (DAMs) primarily accumulate in lipids, organoheterocyclic compounds, organic acids their derivatives. Notably, DAMs exhibiting high peaks include acetylcarnitine, glutathione, linoleic acid, L-propionylcarnitine, among others. transcriptome analysis differentially expressed genes (DEGs) expression D30 included lnaA, af470, ZEB1; D24 comprised SPBC29A3.09c YBT1; D36 dtxS1, PA1538, katG. combined revealed significant extremely positive negative correlations between all DEGs. primary enriched pathways (p < 0.05) glutathione metabolism, tryptophan carbon biosynthesis secondary phenylalanine metabolism. metabolic pathway map DEGs influencing activity were significantly up-regulated but down-regulated D36. correlation suggests an increase content promotes levels enzyme non-enzyme substances, ultimately enhancing sinensis. These findings serve as reference how affect This may contribute enhanced development application

Язык: Английский

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Influence of Drying Methods on the Morphological Features, Microstructural Properties, and Antioxidant Performance of Floccularia luteovirens: A Metabolomic Analysis

Journal of Fungi, Год журнала: 2025, Номер 11(1), С. 78 - 78

Опубликована: Янв. 19, 2025

Floccularia luteovirens (F. luteovirens) has garnered increasing attention as an ingredient in both the pharmaceutical and food industries. Depending on drying method, accumulation of metabolites can greatly affect quality. This research employed untargeted metabolomics (LC-MS/MS) strategy to elucidate similarities differences morphological characteristics, microstructure, antioxidant capacity, metabolic profiles F. subjected three distinct methods: natural air-drying (YG), oven-drying (HG), vacuum freeze-drying (DG). Our findings indicated that color samples dried using YG HG methods was yellow-brown, exhibiting a high degree browning, whereas processed by DG method displayed golden-yellow hue desirable fullness. Regarding from exhibited small unevenly distributed pores, contrast which were structurally intact characterized large inter-tissue pores. The activity samples, found be significantly superior compared two other methods. A correlation analysis significant link between capacity lipid well lipid-like molecules. Metabolomic identified 1617 across 15 superclasses, with lipids, molecules, organic acids derivatives, heterocyclic compounds being predominant luteovirens. Furthermore, KEGG enrichment highlighted 20 pathways, indicating metabolism amino could involved processes linked clarifies how different techniques impact or pathways luteovirens, identifying mechanisms influence its quality providing reference for optimizing processing storage.

Язык: Английский

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Effects of Different Drying Processes on Bioactive Components, Volatile Compounds, and In Vitro Inhibition of Starch Digestion in Mulberry Leaf Extracts

Foods, Год журнала: 2025, Номер 14(6), С. 998 - 998

Опубликована: Март 14, 2025

The significant demand for medicinal plants with special efficacy has prompted us to adopt appropriate processing methods enhance the nutritional quality and flavor of raw materials. This study evaluated impacts freeze-drying (FD), hot-air drying (HAD), spray (SD) on bioactive compounds, characteristics, inhibition starch digestion in mulberry leaf ethanol extract (MLE). Results indicated that FDMLE exhibited highest total alkaloids content (TAC: 0.14 ± 0.02 mg/g) flavonoid (TFC: 19.32 0.58 mg/g), along inhibitory effects hydrolysis at 180 min (starch rate <50%). microstructure HADMLE was closest powder (ML), but SD better preserved color ML (ΔE = 1.55 0.04). Combined electronic nose gas chromatography-ion mobility spectrometry (GC-IMS) found HAD facilitated conversion precursors into Ethyl formate, rose oxide, (Z)-3-hexenol (M). SDMLE contained higher levels pentanal, (E)-2-hexenal (D), (E)-2-pentanone, 3-Methyl-2-butenal ethyl butyrate, 1-penten-3-one (D). diversity novel volatile compounds (VOCs), 18 newly identified species. In conclusion, FD is a potential method effectively reduce degradation MLE during process.

Язык: Английский

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Effect of freeze-pressure regulated extraction technology on the physicochemical properties and pharmacological activities of guizhi extract

Frontiers in Chemistry, Год журнала: 2025, Номер 13

Опубликована: Апрель 25, 2025

Extraction is the core process for obtaining bioactive compounds from medicinal plants. Enhancing extraction efficiency of aromatic herbs has become a critical challenge. This study introduced novel freeze-pressure regulated (FE) technique to improve Gui Zhi (GZ). Compared traditional methods, FE yielded significantly lower pH 4.74, higher zeta potential −13.93 mV, and smaller average particle size 304.57 nm. Scanning electron microscopy (SEM) mercury intrusion porosimetry (MIP) confirmed that creates larger pores an expanded surface area, facilitating more effective compound release. HPLC analysis indicated increased cinnamaldehyde content 348.53 370.20 μg/g. UPLC-MS further demonstrated extracting volatile phenolic compounds. Furthermore, therapeutic effect GZ extract on wind-cold syndrome model was investigated. alleviated symptoms restored lung tissue integrity, through regulation citric acid cycle thiamine metabolism pathways. The findings not only support application technology in herbal but also offer approaches efficient utilization like modern medicine.

Язык: Английский

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Effects of complex cryoprotectant on the freeze-drying survival of Lactobacillus acidophilus FMNS-10 and its protective mechanisms

Journal of Stored Products Research, Год журнала: 2025, Номер 112, С. 102646 - 102646

Опубликована: Апрель 26, 2025

Язык: Английский

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Evaluation of Lipid Changes During the Drying Process of Cordyceps sinensis by Ultra Performance Liquid Chromatography–Tandem Mass Spectrometry (UPLC-MS/MS)-Based Lipidomics Technique

Journal of Fungi, Год журнала: 2024, Номер 10(12), С. 855 - 855

Опубликована: Дек. 11, 2024

Comprehensive analysis of the lipid content in

Язык: Английский

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Research progress on glucose-regulating mechanism of food-derived polysaccharides

Food Bioscience, Год журнала: 2024, Номер 63, С. 105679 - 105679

Опубликована: Дек. 11, 2024

Язык: Английский

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