Single-Cell RNA Sequencing Reveals an Atlas of Meihua Pig Testis Cells

Animals, Год журнала: 2025, Номер 15(5), С. 752 - 752

Опубликована: Март 5, 2025

Mammalian spermatogenesis is a complex biological process that regulated by multiple types of cells. The heterogeneity these cells poses challenge for analyzing different cell at developmental stages. To characterize the transcriptomic landscape porcine and identify potential marker genes spermatogonia, an unbiased study in neonatal sexually mature six-month-old Meihua pigs was performed using 10× Genomics single-cell RNA sequencing (scRNA-seq). Through collection scRNA-seq data from 13,839 pig testes, three germ (spermatogonia, spermatocytes spermatids) eight somatic (Sertoli cells, Leydig myoid/stromal endothelial T cells/macrophages erythroblasts) were identified. Pseudo-timing analysis showed myoid stromal originated common progenitors pigs. Functional enrichment revealed differentially expressed (DEGs) testicular enriched pathways Ribosome, Oxidative phosphorylation, Protein processing endoplasmic reticulum, Retrograde endocannabinoid signaling, Cellular senescence Insulin signaling. Meanwhile, except which same as first DEGs also Spliceosome, Cell cycle, Autophagy Mitophagy pathways. Furthermore, candidate gene TKTL1 spermatogonia identified immunohistochemistry immunofluorescence. In conclusion, we collected transcription datasets constructed maps during development pigs, provided new insights into various their testes.

Язык: Английский

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Identification of Candidate Genes Related to Hybrid Sterility by Genomic Structural Variation and Transcriptome Analyses in Cattle-yak

Journal of Dairy Science, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

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Overexpression of TAF4B Promoted the Proliferation of Undifferentiated Spermatogonia in Cattleyak In Vitro

Reproduction in Domestic Animals, Год журнала: 2024, Номер 59(11)

Опубликована: Ноя. 1, 2024

ABSTRACT As the hybrid between cattle and yak, cattleyak is a typical male sterile mammal, underlying mechanism for its spermatogenic arrest still unclear. In this study, coding region of TAF4B gene was cloned by RT‐PCR analysed bioinformatics. To investigate effects on cellular proliferation differentiation, an expression vector generated introduced into undifferentiated spermatogonia (UDSPG) cattleyak. The results showed that protein encoded did not contain signal peptide sequence. level in UDSPG lower than while overexpression promoted activity UDSPG. Meanwhile, meiosis‐related genes increased but differentiation‐related were decreased. Therefore, aberrant possibly impaired differentiation equilibrium decreased growth potentiality, thereby reducing quantity affecting spermatogenesis. This study provided potential approach further elucidation spermatogenesis new idea solving problem infertility

Язык: Английский

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N6-Methyladenosine Modification-Related Genes Express Differentially in Sterile Male Cattle-Yaks

Life, Год журнала: 2024, Номер 14(9), С. 1155 - 1155

Опубликована: Сен. 12, 2024

N

Язык: Английский

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Full-length transcriptome sequencing of the longissimus dorsi muscle of yak and cattle-yak using nanopore technology

International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 138071 - 138071

Опубликована: Ноя. 1, 2024

Язык: Английский

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