Decay of RNA and Infectious SARS-CoV-2 and Murine Hepatitis Virus in Wastewater DOI
Kevin Purves,

Liam J. Reynolds,

Laura Sala‐Comorera

и другие.

Опубликована: Янв. 1, 2023

Wastewater-based epidemiology (WBE) has been an important tool for population surveillance during the COVID-19 pandemic and continues to play a key role in monitoring SARS-CoV-2 infection levels following reductions national clinical testing schemes. Studies measuring decay profiles of wastewater have underscored value WBE, however investigations hampered by high biosafety requirements studies. Therefore, surrogate viruses with lower standards used studies, such as murine hepatitis virus (MHV), but few studies directly compared rates both viruses. We persistence MHV wastewater, using TCID50 RT-qPCR assays assess infectious titre viral gene markers, respectively. Infectious indicate similar endpoints, observed early characteristics differed, decaying more rapidly than MHV, raising questions about suitability wastewater. This study further highlights importance ongoing other pathogens sample preparation rate overestimation.

Язык: Английский

Clinical characteristics and phylogenetic analysis of human enteric adenovirus type 41 (HAdV-F41) from children with gastroenteritis during SARS-CoV-2 pandemic DOI Creative Commons
Ru Bai,

Yanyuan Chen,

Junxian Ou

и другие.

Infection Genetics and Evolution, Год журнала: 2024, Номер 123, С. 105619 - 105619

Опубликована: Июнь 19, 2024

Human adenovirus type 41 (HAdV-F41) usually causes pediatrics gastroenteritis. However, it was reported to be associated with the outbreaks of severe acute hepatitis unknown aetiology (SAHUA) in during COVID-19 pandemic. In this study, we investigated prevalence enteric HAdV-F41 37,920 paediatric gastroenteritis cases from 2017 2022 Guangzhou, China. All children presented were tested negative for SARS-CoV-2 "zero-COVID" period. The main clinical symptom diarrhea (96.5%). No fatalities nor liver abnormal symptoms found. 2021, one year since pandemic COVID-19, abruptly increased 3.71% 8.64% (P < 0.001). circulating worldwide classified into eight different subtypes (G1-G8) based on phylogenetic clustering permutation four capsid genes HAdV-F41. G3 predominant subtype (56.2%; 77/137). CRV5 isolates SAHUA belong subtype, which N312D and H335D mutations short fiber knob identified both Guangzhou isolates, presumably changing virus tropism by directly interacting heparin sulfate (HS) receptor. Additionally, a novel recombinant G6 is unique only China first study. This study highlighting viral evolution finding provide insight characteristics infections as well uncertain role cause SAHUA.

Язык: Английский

Процитировано

1

Evaluation of qPCR for the Selective Detection of Enteric Adenovirus Followed by Sequence-Based Genetic Characterization of F Strains Circulating in Brazil DOI Creative Commons
Lilian Gonçalves do Nascimento,

Sylvia Kahwage Sarmento,

Reinaldo Röpke

и другие.

Applied Microbiology, Год журнала: 2024, Номер 4(3), С. 1016 - 1029

Опубликована: Июнь 27, 2024

Human adenovirus (HAdV) F40/41 is an important pathogen in pediatric acute gastroenteritis cases. However, the diversity of study designs and diagnostic methods often leads to misinterpretations their impact. Our explored genetic HAdV-F40/41 Brazil using a specific qPCR assay for HAdV species F, combined with phylogenetic analysis partial hexon fiber genes. results demonstrated that HAdV-F41 strains predominated exhibited higher than HAdV-F40 strains. Based on gene, Brazilian were grouped into two genome type clusters (GTC), further divided subclusters, most clusteringto GTC2. The shaft region gene conservation among HAdV-F41. F identified HAdV-F additional 31.5% (34/108) previously uncharacterized HAdV-positive samples detected non-specific assay. Both assays strongly correlated detecting HAdV-F, enteric types can enhance surveillance, especially when sequencing not possible. provides novel insights regarding Brazil.

Язык: Английский

Процитировано

1

Association between adeno-associated virus 2 and severe acute hepatitis of unknown etiology in Japanese children DOI
Ken‐ichi Iwata,

Yuka Torii,

Aiko Sakai

и другие.

Journal of Infection and Chemotherapy, Год журнала: 2024, Номер 31(1), С. 102462 - 102462

Опубликована: Июль 4, 2024

Язык: Английский

Процитировано

1

Co-occurrence of adeno-associated virus 2 and human enteric adenovirus (group F) in wastewater after worldwide outbreaks of acute hepatitis of unknown etiology (AHUE) DOI

Roberto Rodríguez,

F. Garza,

Olivia N Birch

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 955, С. 176806 - 176806

Опубликована: Окт. 14, 2024

Язык: Английский

Процитировано

1

Mutated Adenovirus Attacks in West Bengal, India: Risk Evaluation of Multi‐Country Outbreaks and Mitigation Strategies DOI Creative Commons
Md. Ashrafur Rahman,

Sydney Cronmiller,

Julianne Ernest

и другие.

Nursing Open, Год журнала: 2024, Номер 11(10)

Опубликована: Окт. 1, 2024

ABSTRACT Aim The human adenovirus (HAdV) is beginning to spread rapidly in children through human, surface and animal vectors. Around 12,000 cases were recognised 2022 West Bengal a shocking number of arose throughout India other under‐developed areas. This going be big threat public health since no vaccine, awareness or protocol policies introduced. Early detection, immediate isolation proper policy developments are the key factors overcoming situation. Therefore, we performed this rapid review discussed probable mitigation strategies, updated research on vaccine development, treatment strategies control outbreaks mutated HAdV. Design narrative publicly available information. Methods Here, extracted information data using terms HAdV outbreaks, mutations, species, risks prevention from Google Scholar PubMed. We considered relevant articles that have ongoing research, antiviral drugs for managing outbreaks. Results detection throat swabs, symptomatic treatments required minimise viral infections. A massive test needs find affected people. should immediately isolated. It recommended treat high‐touch surfaces with heat‐ bleach‐containing cleaners prevent infection. Oxygen support many broad‐spectrum antivirals been used Several studies showed antibody neutralisation interactions between natural killer cell receptor KIR3DS1 HLA‐F infected cells, indicating possible therapeutic options future. HAdV‐4 HAdV‐7 vaccines limitedly approved administration military personnel. Conclusion Isolation, certain safety measures, further new could useful virus producing worldwide pandemic. Also, authorities ensure interventions nursing care facilities children. Patient Public Contribution contribution was not our work.

Язык: Английский

Процитировано

1

Non‐A to E hepatitis in children: Detecting a novel viral epidemic during the COVID‐19 pandemic DOI Creative Commons
Harald Brüssow

Microbial Biotechnology, Год журнала: 2023, Номер 16(10), С. 1879 - 1887

Опубликована: Авг. 21, 2023

Abstract During the COVID‐19 pandemic, two further novel viral epidemics were described in 2022, monkeypox virus infections men having sex with and non‐A to E hepatitis children. The latter occurred first half of 2022 about 1000 cases worldwide, necessitating liver transplantation 5% causing death 2% patients. It took some effort clarify cause epidemic. Researchers confronted a polymicrobial infection consisting an adenovirus‐associated type 2 (AAV2) infection, co‐occurring either human adenovirus 41 (HAdV41) or herpesvirus infections; most prominently 6 (HHV‐6). AAV‐2, small Dependovirus Parvovirus family, needs these helper viruses for its replication. AAV2 is used as vector liver‐targeting gene therapy but was not previously known acute hepatitis. HAdV41 HHV‐6 are mostly diarrhoea febrile illnesses associated skin rashes children, respectively. Except few case reports hepatitis, HAdV major pathogens immunosuppressed A potential role SARS‐CoV‐2 has also been discussed popular hypothesis involves indirect pandemic this disease. Exposure occurs nearly quantitatively during year life. Social distancing measures, followed by lifting measures might have caused delayed exposure multiple, normally benign childhood eliciting dysregulated immune response pathological effects cells. In fall when conditions longer met, numbers dwindled. unequilibrated instead intrinsic cytopathic activity implicated supported enrichment particular HLA allele over controls.

Язык: Английский

Процитировано

3

Two years of longitudinal measurements of human adenovirus group F, norovirus GI and GII, rotavirus, enterovirus, enterovirus D68, hepatitis A virus,Candida auris, and West Nile virus nucleic-acids in wastewater solids: A retrospective study at two wastewater treatment plants DOI Creative Commons
Alexandria B. Boehm, Marlene K. Wolfe, Bradley J. White

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 24, 2023

Abstract Wastewater monitoring for infectious disease targets is increasingly used to better understand circulation of diseases. The present study validated hydrolysis-probe digital droplet (reverse-transcriptase (RT))-PCR assays important enteric viruses (rotavirus, adenovirus group F, norovirus GI and GII, enteroviruses), outbreak or emerging (hepatitis A West Nile virus), an drug resistant fungal pathogen ( Candida auris ). We the retrospectively measure concentrations in wastewater solids. Viral nucleic-acid were measured two solids samples per week at treatment plants San Francisco Bay Area California, USA 26 months. detected all with exception virus. At both plants, human F was highest concentrations, followed by enteroviruses, GI, rotavirus lowest concentrations. Hepatitis C. less consistently than aforementioned viruses. Enterovirus D68 a limited time frame during fall 2022 sites. measurements reported herein, some cases their seasonal trends, are consistent previous reports these wastewater. These represent first quantitative solid fraction This lays foundation use detection specific programs aimed spread

Язык: Английский

Процитировано

2

Uncovering the Underlying Causes of Severe Acute Hepatitis of Unknown Aetiology in Children: A Comprehensive Review DOI Creative Commons

Maria A. AlShurman,

Bara’ Abdallah AlShurman, Hibah Sehar

и другие.

Dr Sulaiman Al Habib Medical Journal, Год журнала: 2023, Номер 5(4), С. 101 - 117

Опубликована: Окт. 30, 2023

Abstract Background Since October 2021, multiple paediatric cases of severe acute hepatitis unknown aetiology (SAHUA) not caused by A–E viruses have been reported countries. As 14 September 2022, approximately 1296 probable SAHUA in 37 countries and regions had reported. Objectives The purpose this study was to present a complete picture outbreak, including its origin, current cases, clinical signs, possible hypotheses, potential treatments. Methods A thorough search for literature from 2021 2023 performed the PubMed Medline databases. Additional websites, WHO, CDC, ECDC, UKHSA, were searched further relevant data. Results Common symptoms include jaundice, vomiting, pale stools, diarrhoea, abdominal pain, nausea, whereas fever is infrequent. Elevated AST ALT are prevalent, most test positive adenovirus. However, immunohistochemical staining on liver tissue often yields negative results adenovirus, thus challenging hypothesis that adenovirus definitive cause. recent compelling has implicated AAV-2 as likely etiologic agent involving abnormal replication products immune-mediated hepatic disease. Evidence low immunogenicity, tropism, immune responses supports hypothesis. SARS-CoV-2's role also explored. Some SARS-CoV-2 IgG positivity even when PCR tests negative, thereby suggesting silent prior infections. Cidofovir, suggested treatment human infection immunocompromised patients, decreased adenoviral load two cases. Notably, 29 deaths reported, 55 required or received transplant. Conclusion children presents complex challenge with involvement factors. may affect disease severity—a possibility warranting investigation. Treatment options diagnostics, supportive care, antivirals, immunosuppression. Prevention relies control measures, management requires advanced diagnostics international collaboration. remains an enigma, underscoring need continued research adaptability emerging infectious threats.

Язык: Английский

Процитировано

2

Acute hepatitis of unknown etiology in children: Comparison between the U.K., U.S., and Japan, and ingenious clinical practice attempts DOI Open Access

Ryo Sumazaki,

Aiko Sakai,

Sotaro Mushiake

и другие.

Kanzo, Год журнала: 2024, Номер 65(1), С. 1 - 11

Опубликована: Янв. 1, 2024

2022年に欧米で原因不明の小児急性肝炎が急増した.当初は流行中のアデノウイルス(AdV)陽性例が多いと注目されたが,メタゲノム解析により英米の流行期肝炎患者の80%以上で大量のアデノ随伴ウイルス2型(AAV2)が検出された.ヘルパーウイルスとなるAdVやHHV6の同時感染が多いこと,特定のHLA型が多いことも報告され,遺伝素因にAAV2感染が重なり発症する可能性がある.AAV9ベクターを用いる遺伝子治療により免疫介在性の急性肝障害を発症した例が多く,類似の病態と考えられる.また原因不明肝炎の一部にはSARS-CoV-2関連症例も含まれる.日本では1年弱で本症が162例届け出されたが,パンデミック以前と比べると減少傾向である.AAV2検出例も10%程度と少ないことから,欧米の流行とは異なる.従来から小児急性肝不全の約40%が原因不明であり,その一部にAAV2が関与していることが推測される.

Язык: Английский

Процитировано

0

Decay of RNA and infectious SARS-CoV-2 and murine hepatitis virus in wastewater DOI
Kevin Purves,

Liam J. Reynolds,

Laura Sala-Comorera

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 944, С. 173877 - 173877

Опубликована: Июнь 12, 2024

Язык: Английский

Процитировано

0