Decay of RNA and Infectious SARS-CoV-2 and Murine Hepatitis Virus in Wastewater DOI
Kevin Purves,

Liam J. Reynolds,

Laura Sala‐Comorera

et al.

Published: Jan. 1, 2023

Wastewater-based epidemiology (WBE) has been an important tool for population surveillance during the COVID-19 pandemic and continues to play a key role in monitoring SARS-CoV-2 infection levels following reductions national clinical testing schemes. Studies measuring decay profiles of wastewater have underscored value WBE, however investigations hampered by high biosafety requirements studies. Therefore, surrogate viruses with lower standards used studies, such as murine hepatitis virus (MHV), but few studies directly compared rates both viruses. We persistence MHV wastewater, using TCID50 RT-qPCR assays assess infectious titre viral gene markers, respectively. Infectious indicate similar endpoints, observed early characteristics differed, decaying more rapidly than MHV, raising questions about suitability wastewater. This study further highlights importance ongoing other pathogens sample preparation rate overestimation.

Language: Английский

Association between adeno-associated virus 2 and severe acute hepatitis of unknown etiology in Japanese children DOI
Ken‐ichi Iwata,

Yuka Torii,

Aiko Sakai

et al.

Journal of Infection and Chemotherapy, Journal Year: 2024, Volume and Issue: 31(1), P. 102462 - 102462

Published: July 4, 2024

Language: Английский

Citations

1
Co-occurrence of adeno-associated virus 2 and human enteric adenovirus (group F) in wastewater after worldwide outbreaks of acute hepatitis of unknown etiology (AHUE) DOI

Roberto Rodríguez,

F. Garza,

Olivia N Birch

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 955, P. 176806 - 176806

Published: Oct. 14, 2024

Language: Английский

Citations

1
Mutated Adenovirus Attacks in West Bengal, India: Risk Evaluation of Multi‐Country Outbreaks and Mitigation Strategies DOI Creative Commons
Md. Ashrafur Rahman,

Sydney Cronmiller,

Julianne Ernest

et al.

Nursing Open, Journal Year: 2024, Volume and Issue: 11(10)

Published: Oct. 1, 2024

ABSTRACT Aim The human adenovirus (HAdV) is beginning to spread rapidly in children through human, surface and animal vectors. Around 12,000 cases were recognised 2022 West Bengal a shocking number of arose throughout India other under‐developed areas. This going be big threat public health since no vaccine, awareness or protocol policies introduced. Early detection, immediate isolation proper policy developments are the key factors overcoming situation. Therefore, we performed this rapid review discussed probable mitigation strategies, updated research on vaccine development, treatment strategies control outbreaks mutated HAdV. Design narrative publicly available information. Methods Here, extracted information data using terms HAdV outbreaks, mutations, species, risks prevention from Google Scholar PubMed. We considered relevant articles that have ongoing research, antiviral drugs for managing outbreaks. Results detection throat swabs, symptomatic treatments required minimise viral infections. A massive test needs find affected people. should immediately isolated. It recommended treat high‐touch surfaces with heat‐ bleach‐containing cleaners prevent infection. Oxygen support many broad‐spectrum antivirals been used Several studies showed antibody neutralisation interactions between natural killer cell receptor KIR3DS1 HLA‐F infected cells, indicating possible therapeutic options future. HAdV‐4 HAdV‐7 vaccines limitedly approved administration military personnel. Conclusion Isolation, certain safety measures, further new could useful virus producing worldwide pandemic. Also, authorities ensure interventions nursing care facilities children. Patient Public Contribution contribution was not our work.

Language: Английский

Citations

1
A minimal transcription template-based amplification-free CRISPR-Cas13a strategy for DNA detection DOI

Tianchi Zhuang,

Gao Chang,

Wenwu Zhao

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 270, P. 116918 - 116918

Published: Nov. 17, 2024

Language: Английский

Citations

1
Non‐A to E hepatitis in children: Detecting a novel viral epidemic during the COVID‐19 pandemic DOI Creative Commons
Harald Brüssow

Microbial Biotechnology, Journal Year: 2023, Volume and Issue: 16(10), P. 1879 - 1887

Published: Aug. 21, 2023

Abstract During the COVID‐19 pandemic, two further novel viral epidemics were described in 2022, monkeypox virus infections men having sex with and non‐A to E hepatitis children. The latter occurred first half of 2022 about 1000 cases worldwide, necessitating liver transplantation 5% causing death 2% patients. It took some effort clarify cause epidemic. Researchers confronted a polymicrobial infection consisting an adenovirus‐associated type 2 (AAV2) infection, co‐occurring either human adenovirus 41 (HAdV41) or herpesvirus infections; most prominently 6 (HHV‐6). AAV‐2, small Dependovirus Parvovirus family, needs these helper viruses for its replication. AAV2 is used as vector liver‐targeting gene therapy but was not previously known acute hepatitis. HAdV41 HHV‐6 are mostly diarrhoea febrile illnesses associated skin rashes children, respectively. Except few case reports hepatitis, HAdV major pathogens immunosuppressed A potential role SARS‐CoV‐2 has also been discussed popular hypothesis involves indirect pandemic this disease. Exposure occurs nearly quantitatively during year life. Social distancing measures, followed by lifting measures might have caused delayed exposure multiple, normally benign childhood eliciting dysregulated immune response pathological effects cells. In fall when conditions longer met, numbers dwindled. unequilibrated instead intrinsic cytopathic activity implicated supported enrichment particular HLA allele over controls.

Language: Английский

Citations

3
Two years of longitudinal measurements of human adenovirus group F, norovirus GI and GII, rotavirus, enterovirus, enterovirus D68, hepatitis A virus,Candida auris, and West Nile virus nucleic-acids in wastewater solids: A retrospective study at two wastewater treatment plants DOI Creative Commons
Alexandria B. Boehm, Marlene K. Wolfe, Bradley J. White

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 24, 2023

Abstract Wastewater monitoring for infectious disease targets is increasingly used to better understand circulation of diseases. The present study validated hydrolysis-probe digital droplet (reverse-transcriptase (RT))-PCR assays important enteric viruses (rotavirus, adenovirus group F, norovirus GI and GII, enteroviruses), outbreak or emerging (hepatitis A West Nile virus), an drug resistant fungal pathogen ( Candida auris ). We the retrospectively measure concentrations in wastewater solids. Viral nucleic-acid were measured two solids samples per week at treatment plants San Francisco Bay Area California, USA 26 months. detected all with exception virus. At both plants, human F was highest concentrations, followed by enteroviruses, GI, rotavirus lowest concentrations. Hepatitis C. less consistently than aforementioned viruses. Enterovirus D68 a limited time frame during fall 2022 sites. measurements reported herein, some cases their seasonal trends, are consistent previous reports these wastewater. These represent first quantitative solid fraction This lays foundation use detection specific programs aimed spread

Language: Английский

Citations

2
Uncovering the Underlying Causes of Severe Acute Hepatitis of Unknown Aetiology in Children: A Comprehensive Review DOI Creative Commons

Maria A. AlShurman,

Bara’ Abdallah AlShurman, Hibah Sehar

et al.

Dr Sulaiman Al Habib Medical Journal, Journal Year: 2023, Volume and Issue: 5(4), P. 101 - 117

Published: Oct. 30, 2023

Abstract Background Since October 2021, multiple paediatric cases of severe acute hepatitis unknown aetiology (SAHUA) not caused by A–E viruses have been reported countries. As 14 September 2022, approximately 1296 probable SAHUA in 37 countries and regions had reported. Objectives The purpose this study was to present a complete picture outbreak, including its origin, current cases, clinical signs, possible hypotheses, potential treatments. Methods A thorough search for literature from 2021 2023 performed the PubMed Medline databases. Additional websites, WHO, CDC, ECDC, UKHSA, were searched further relevant data. Results Common symptoms include jaundice, vomiting, pale stools, diarrhoea, abdominal pain, nausea, whereas fever is infrequent. Elevated AST ALT are prevalent, most test positive adenovirus. However, immunohistochemical staining on liver tissue often yields negative results adenovirus, thus challenging hypothesis that adenovirus definitive cause. recent compelling has implicated AAV-2 as likely etiologic agent involving abnormal replication products immune-mediated hepatic disease. Evidence low immunogenicity, tropism, immune responses supports hypothesis. SARS-CoV-2's role also explored. Some SARS-CoV-2 IgG positivity even when PCR tests negative, thereby suggesting silent prior infections. Cidofovir, suggested treatment human infection immunocompromised patients, decreased adenoviral load two cases. Notably, 29 deaths reported, 55 required or received transplant. Conclusion children presents complex challenge with involvement factors. may affect disease severity—a possibility warranting investigation. Treatment options diagnostics, supportive care, antivirals, immunosuppression. Prevention relies control measures, management requires advanced diagnostics international collaboration. remains an enigma, underscoring need continued research adaptability emerging infectious threats.

Language: Английский

Citations

2
Acute hepatitis of unknown etiology in children: Comparison between the U.K., U.S., and Japan, and ingenious clinical practice attempts DOI Open Access

Ryo Sumazaki,

Aiko Sakai,

Sotaro Mushiake

et al.

Kanzo, Journal Year: 2024, Volume and Issue: 65(1), P. 1 - 11

Published: Jan. 1, 2024

2022年に欧米で原因不明の小児急性肝炎が急増した.当初は流行中のアデノウイルス(AdV)陽性例が多いと注目されたが,メタゲノム解析により英米の流行期肝炎患者の80%以上で大量のアデノ随伴ウイルス2型(AAV2)が検出された.ヘルパーウイルスとなるAdVやHHV6の同時感染が多いこと,特定のHLA型が多いことも報告され,遺伝素因にAAV2感染が重なり発症する可能性がある.AAV9ベクターを用いる遺伝子治療により免疫介在性の急性肝障害を発症した例が多く,類似の病態と考えられる.また原因不明肝炎の一部にはSARS-CoV-2関連症例も含まれる.日本では1年弱で本症が162例届け出されたが,パンデミック以前と比べると減少傾向である.AAV2検出例も10%程度と少ないことから,欧米の流行とは異なる.従来から小児急性肝不全の約40%が原因不明であり,その一部にAAV2が関与していることが推測される.

Language: Английский

Citations

0
Decay of RNA and infectious SARS-CoV-2 and murine hepatitis virus in wastewater DOI
Kevin Purves,

Liam J. Reynolds,

Laura Sala-Comorera

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 944, P. 173877 - 173877

Published: June 12, 2024

Language: Английский

Citations

0
Adenovirus an Increasing Concern in West Bengal - Manifestation, Detection, Prevention and Treatment DOI Open Access

Bijurica Chakraborty,

Sounik Sarka,

Anowara Gazi

et al.

ACTA SCIENTIFIC MICROBIOLOGY, Journal Year: 2023, Volume and Issue: 6(5), P. 74 - 78

Published: May 1, 2023

Human Adenovirus (hAdv) infections are currently at a surge in India with West Bengal holding prominent place.Patient cohort majority being children getting reported flue like symptoms, low oxygen level and upper respiratory tract infection.Diarrhoea pink eyes also noted.Clinical manifestations by different serotypes of the aforementioned DNA virus were previously confined to multifaceted mild symptoms but recently taking serious toll mainly due mutant varieties virus.Since contamination spreads through droplet infection faecal-oral route, personal hygiene holds most superior preventive measure.Patient infective swabs, stool and/or blood can be tested for presence hAdv diagnostic methods, PCR based techniques commonly followed pathological laboratories.Number patients both paediatric adults admitted hospitals increasing reports infection.Treatments specific clinical provided cases, whereas certain drugs Ganciclovir Ribavirin used treat severe infections, variable success rate.Drug Cidofovir had shown some degree moderate treatment immune-compromised patients.However, no has been till date infection.

Language: Английский

Citations

0