Lab on a Chip, Год журнала: 2024, Номер 24(20), С. 4816 - 4826
Опубликована: Янв. 1, 2024
The new lab-on-a-chip design allows organoids of different origin to be cultured and vascularized on CAM transferred them in vitro conditions.
Язык: Английский
Cells, Год журнала: 2023, Номер 12(19), С. 2368 - 2368
Опубликована: Сен. 27, 2023
Induced pluripotent stem cell (iPSC) technology enables differentiation of human hepatocytes or hepatocyte-like cells (iPSC-HLCs). Advances in 3D culturing platforms enable the development more vivo-like liver models that recapitulate complex architecture and functionality better than traditional 2D monocultures. Moreover, within liver, non-parenchymal (NPCs) are critically involved regulation maintenance hepatocyte metabolic function. Thus, combining culture co-culturing various types potentially create functional vitro Here, we report establishment cultures iPSC-HLCs alone co-culture with umbilical vein endothelial (HUVECs) adipose tissue-derived mesenchymal stem/stromal (hASCs). The were performed as spheroids on microfluidic chips utilizing biomaterials. Our results show both spheroid on-chip enhance expression mature marker genes proteins compared to 2D. Among models, saw best iPSC-HLC monoculture spheroids. On contrary, chip system, multilineage model outperformed model. Additionally, optical projection tomography (OPT) electrical impedance (EIT) system revealed changes size conductivity during culture, suggesting cell-cell connections. Altogether, present study demonstrates can successfully be cultured chips, NPCs enhances their functionality. These systems promising human-derived usable liver-related studies, specifically when using patient-specific iPSCs.
Язык: Английский
Процитировано
3
Cells, Год журнала: 2022, Номер 11(9), С. 1574 - 1574
Опубликована: Май 7, 2022
Mesenchymal stem/stromal cells (MSCs) are self-renewing and multipotent progenitors, which constitute the main cellular compartment of bone marrow stroma. Because MSCs have an important role in pathogenesis multiple myeloma, it is essential to know if novel drugs target MSCs. Melflufen a anticancer peptide–drug conjugate compound for patients with relapsed refractory myeloma. Here, we studied cytotoxicity melflufen, melphalan doxorubicin healthy human marrow-derived (BMSCs) how these affect BMSC proliferation. We established co-cultures BMSCs MM.1S myeloma see increase or decrease melphalan, bortezomib doxorubicin. evaluated differentiation into adipocytes osteoblasts BMSC-supported formation vascular networks. Our results showed that were more sensitive melflufen than melphalan. The was not affected by co-culture BMSCs, as case Adipogenesis, osteogenesis BMSC-mediated angiogenesis all melflufen. Melphalan similar ways. effects on adipogenesis solely because proliferation, seen from differential expression markers normalized cell number. Overall, our indicate has significant impact could possibly therapy outcome.
Язык: Английский
Процитировано
5
Frontiers in Cardiovascular Medicine, Год журнала: 2023, Номер 10
Опубликована: Окт. 17, 2023
Cardiometabolic diseases and cancer are among the most common worldwide a serious concern to healthcare system. These conditions, apparently distant, share molecular cellular determinants, that can represent targets for preventive therapeutic approaches. The bone marrow plays an important role in this context as it is main source of cells involved cardiovascular regeneration, one sites liquid solid tumor metastasis, both characterized by trafficking across vasculature. vasculature has been widely studied animal models, however, clear need human-specific vitro resemble lined endothelial study mechanisms governing cell trafficking. In review, we summarized current knowledge on models developed research.
Язык: Английский
Процитировано
1
ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 10(9), С. 5513 - 5536
Опубликована: Авг. 22, 2024
Irreversible bone defects resulting from trauma, infection, and degenerative illnesses have emerged as a significant health concern. Structurally functionally controllable hydrogels made by tissue engineering (BTE) become promising biomaterials. Natural proteins are able to establish connections with autologous through unique biologically active regions. Hydrogels based on can simulate the microenvironment regulate biological behavior of stem cells in niche, making them candidates for research related regeneration. This article reviews functions various natural macromolecular (such collagen, gelatin, fibrin, silk fibroin) highlights their special advantages hydrogels. Then latest trends cross-linking modified protein improved mechanical properties composite loaded exogenous micromolecular been discussed. Finally, applications hydrogels, such 3D printed microspheres, injectable were introduced, aiming provide reference repair clinical defects.
Язык: Английский
Процитировано
0
Lab on a Chip, Год журнала: 2024, Номер 24(20), С. 4816 - 4826
Опубликована: Янв. 1, 2024
The new lab-on-a-chip design allows organoids of different origin to be cultured and vascularized on CAM transferred them in vitro conditions.
Язык: Английский
Процитировано
0