Cell signaling and transcriptional regulation of osteoblast lineage commitment, differentiation, bone formation, and homeostasis

Cell Discovery, Год журнала: 2024, Номер 10(1)

Опубликована: Июль 2, 2024

Abstract The initiation of osteogenesis primarily occurs as mesenchymal stem cells undergo differentiation into osteoblasts. This process plays a crucial role in bone formation and homeostasis is regulated by two intricate processes: cell signal transduction transcriptional gene expression. Various essential signaling pathways, including Wnt, BMP, TGF-β, Hedgehog, PTH, FGF, Ephrin, Notch, Hippo, Piezo1/2, play critical facilitating osteoblast differentiation, formation, homeostasis. Key factors this include Runx2, Cbfβ, Runx1, Osterix, ATF4, SATB2, TAZ/YAP. Furthermore, diverse array epigenetic also roles at the level. review provides an overview latest developments current comprehension concerning pathways signaling, regulation hormones, genes involved commitment lineage, well maintenance paper reviews via mechanisms, such histone DNA modifications. Additionally, we summarize biology spurred recent advancements various modern technologies bioinformatics. By synthesizing these insights comprehensive understanding further clarification mechanisms underlying lineage commitment, highlights potential new therapeutic applications for treatment diseases.

Язык: Английский

---

Significance of mechanical loading in bone fracture healing, bone regeneration, and vascularization

Journal of Tissue Engineering, Год журнала: 2023, Номер 14

Опубликована: Янв. 1, 2023

In 1892, J.L. Wolff proposed that bone could respond to mechanical and biophysical stimuli as a dynamic organ. This theory presents unique opportunity for investigations on its potential aid in tissue repair. Routine activities such exercise or machinery application can exert loads bone. Previous research has demonstrated loading affect the differentiation development of mesenchymal tissue. However, extent which stimulation help repair generate related mechanisms remain unclear. Four key cell types tissue, including osteoblasts, osteoclasts, lining cells, osteocytes, play critical roles responding stimuli, while other lineages myocytes, platelets, fibroblasts, endothelial chondrocytes also exhibit mechanosensitivity. Mechanical regulate biological functions through mechanosensor cells intraosseously, making it target fracture healing regeneration. review aims clarify these issues explain remodeling, structure dynamics, mechano-transduction processes response loading. Loading different magnitudes, frequencies, types, versus static loads, are analyzed determine effects cellular function. Finally, importance vascularization nutrient supply regeneration was further discussed.

Язык: Английский

---

Impact of the host response and osteoblast lineage cells on periodontal disease

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Окт. 11, 2022

Periodontitis involves the loss of connective tissue attachment and alveolar bone. Single cell RNA-seq experiments have provided new insight into how resident cells infiltrating immune function in response to bacterial challenge periodontal tissues. Periodontal disease is induced by a combined innate adaptive dysbiosis that initiated including epithelial fibroblasts, which recruit cells. Chemokines cytokines stimulate recruitment osteoclast precursors osteoclastogenesis TNF, IL-1β, IL-6, IL-17, RANKL other factors. Inflammation also suppresses coupled bone formation limit repair osteolytic lesions. Bone lining cells, osteocytes ligament play key role both processes. The contains exhibit similarities tendon osteoblast-lineage mesenchymal stem consisting osteoprogenitors osteoblasts are influenced through MCSF RANKL, directly induce osteoclastogenesis. Following resorption, factors released from resorbed matrix osteoclasts osteal macrophages osteoblast surface. Osteoblast differentiation regulated multiple signaling pathways Wnt, Notch, FGF, IGF-1, BMP, Hedgehog pathways. Diabetes, cigarette smoking aging enhance pathologic processes increase resorption inhibit accelerate loss. Other pathologies such as rheumatoid arthritis, post-menopausal osteoporosis unloading/disuse affect lineage participate lesions promoting inhibiting formation. Thus, periodontitis activation an inflammatory large number osseous

Язык: Английский

---

Integrating physicomechanical and biological strategies for BTE: biomaterials-induced osteogenic differentiation of MSCs

Theranostics, Год журнала: 2023, Номер 13(10), С. 3245 - 3275

Опубликована: Янв. 1, 2023

Large bone defects are a major global health concern.Bone tissue engineering (BTE) is the most promising alternative to avoid drawbacks of autograft and allograft bone.Nevertheless, how precisely control stem cell osteogenic differentiation has been long-standing puzzle.Compared with biochemical cues, physicomechanical stimuli have widely studied for their biosafety stability.The mechanical properties various biomaterials (polymers, bioceramics, metal alloys) become main source stimuli.By altering stiffness, viscoelasticity, topography materials, different strengths transmit into precise signals that mediate differentiation.In addition, externally forces also play critical role in promoting osteogenesis, such as compression stress, tensile fluid shear stress vibration, etc.When exposed forces, mesenchymal cells (MSCs) differentiate lineages by sensing through sensors, including integrin focal adhesions (FAs), cytoskeleton, primary cilium, ions channels, gap junction, activating osteogenic-related mechanotransduction pathways, yes associated proteins (YAP)/TAZ, MAPK, Rho-GTPases, Wnt/β-catenin, TGFβ superfamily, Notch signaling.This review summarizes signals, directly regulate MSCs differentiation, transduction mechanisms MSCs.This provides deep broad understanding discusses challenges remained clinical translocation well outlook future improvements.

Язык: Английский

---

Modeling stress–strain state of cancellous bone tissue fragments in compression

Mechanics of Advanced Materials and Structures, Год журнала: 2025, Номер unknown, С. 1 - 23

Опубликована: Янв. 7, 2025

The article examines the stress–strain state of model cancellous bone tissue fragments under compression, replicating architecture natural bone. study focuses on impact principal trabeculae length and mineral content effective elastic modulus. Findings show that deformation response varies with structure mass, significant normal strains von Mises stress localized in surface layers shorter trabeculae. modulus decreases length. results underscore need to explore additional parameters for designing mechanically compatible osteoimplants. Model align behavior.

Язык: Английский

---

Bone-brain interaction: mechanisms and potential intervention strategies of biomaterials

Bone Research, Год журнала: 2025, Номер 13(1)

Опубликована: Март 17, 2025

Abstract Following the discovery of bone as an endocrine organ with systemic influence, bone-brain interaction has emerged a research hotspot, unveiling complex bidirectional communication between and brain. Studies indicate that brain can influence each other’s homeostasis via multiple pathways, yet there is dearth systematic reviews in this area. This review comprehensively examines interactions across three key areas: bone-derived factors on function, effects brain-related diseases or injuries (BRDI) health, concept skeletal interoception. Additionally, discusses innovative approaches biomaterial design inspired by mechanisms, aiming to facilitate through materiobiological aid treatment neurodegenerative bone-related diseases. Notably, integration artificial intelligence (AI) highlighted, showcasing AI’s role expediting formulation effective targeted strategies. In conclusion, offers vital insights into mechanisms suggests advanced harness these clinical practice. These offer promising avenues for preventing treating impacting skeleton brain, underscoring potential interdisciplinary enhancing human health.

Язык: Английский

---

Three-dimensional bioprinting of artificial blood vessel: Process, bioinks, and challenges

International Journal of Bioprinting, Год журнала: 2023, Номер 9(4), С. 740 - 740

Опубликована: Апрель 28, 2023

The coronary artery bypass grafting is a main treatment for restoring the blood supply to ischemic site by bypassing narrow part, thereby improving heart function of patients. Autologous vessels are preferred in grafting, but their availability often limited due underlying disease. Thus, tissue-engineered vascular grafts that devoid thrombosis and have mechanical properties comparable those natural urgently required clinical applications. Most commercially available artificial implants made from polymers, which prone restenosis. biomimetic vessel containing tissue cells most ideal implant material. Due its precision control ability, three-dimensional (3D) bioprinting promising method prepare system. In 3D process, bioink at core state building topological structure keeping cell viable. Therefore, this review, basic viable materials discussed, research polymers bioink, including decellularized extracellular matrix, hyaluronic acid, collagen, emphasized. Besides, advantages alginate Pluronic F127, mainstream sacrificial material during preparation graft, also reviewed. Finally, an overview applications field presented.

Язык: Английский

---

3D printed Nanohydroxyapatite/Polyamide 66 scaffolds with balanced mechanical property and osteogenic ability for bone repair

Materials & Design, Год журнала: 2024, Номер 241, С. 112896 - 112896

Опубликована: Апрель 1, 2024

The biomaterial requirements for bone tissue repair are extremely strict. It not only requires the to have good biocompatibility and biological activity, but also sufficient mechanical strength. Polyamide 66 (PA66) nano-hydroxyapatite (n-HA) been widely investigated in engineering scaffold, however, its composites require further research. In this study, 3D printing technology was utilized fabricate personalized n-HA/PA66 scaffold. properties of composite can be adjusted by configuring different raw material components applying porosity. found that 50 % porosity scaffold showed a uniform porous structure compressive strength 33.9 MPa. exhibited excellent while improving properties, vitro cell tests indicated printed promoted differentiation marrow mesenchymal stem cells (BMSCs) into osteoblasts. vivo animal model proved endogenous regeneration rabbit patellar defects, with newly formed volume 20.9 after two months implantation. This research proposed polyamide/nano-hydroxyapatite balanced property osteogenic ability customized repair, it has promising application prospects.

Язык: Английский

---

A review on external physical stimuli with biomaterials for bone repair

Chemical Engineering Journal, Год журнала: 2024, Номер 496, С. 153749 - 153749

Опубликована: Июль 4, 2024

Язык: Английский

---

Biomechanical Effects of Mechanical Stress on Cells Involved in Fracture Healing

Orthopaedic Surgery, Год журнала: 2024, Номер 16(4), С. 811 - 820

Опубликована: Март 4, 2024

Fracture healing is a complex staged repair process in which the mechanical environment plays key role. Bone tissue very sensitive to stress stimuli, and literature suggests that appropriate can promote fracture by altering cellular function. However, coupled involving multiple cell types balance limit each other ensure proper healing. The main cells function during different stages of are different, molecular mechanisms required also different. Most previous studies have used single stimulus on individual mechanosensitive cells, there no relatively uniform standard for size frequency stress. Analyzing underlying effects stimulation metabolic regulation signaling pathways such as bone marrow mesenchymal stem (BMSCs), osteoblasts, chondrocytes, osteoclasts currently challenging research hotspot. Grasping how affects at biology level contribute refined management Therefore, this review, we summarize relevant describe associated with healing, their possible pathways, treatment fractures further development regenerative medicine.

Язык: Английский

---