Calcium Phosphates: A Key to Next‐Generation In Vitro Bone Modeling

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(29)

Опубликована: Авг. 23, 2024

Abstract The replication of bone physiology under laboratory conditions is a prime target behind the development in vitro models. model should be robust enough to elicit an unbiased response when stimulated experimentally, giving reproducible outcomes. In tissue generation majorly requires availability cellular components, presence factors promoting proliferation and differentiation, efficient nutrient supply, supporting matrix for cells anchor – gaining predefined topology. Calcium phosphates (CaP) are difficult ignore while considering above requirements model. Therefore, current review focuses on role CaP developing addressing prerequisites generation. Special emphasis given physico‐chemical properties that promote osteogenesis, angiogenesis provide sufficient mechanical strength load‐bearing applications. Finally, future course action discussed ensure utilization field.

Язык: Английский

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METTL3-mediated m6A modification of SOX4 regulates osteoblast proliferation and differentiation via YTHDF3 recognition

Cellular Signalling, Год журнала: 2024, Номер 115, С. 111038 - 111038

Опубликована: Янв. 7, 2024

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Osteocyte-mediated mechanical response controls osteoblast differentiation and function

Frontiers in Physiology, Год журнала: 2024, Номер 15

Опубликована: Март 11, 2024

Low bone mass is a pervasive global health concern, with implications for osteoporosis, frailty, disability, and mortality. Lifestyle factors, including sedentary habits, metabolic dysfunction, an aging population, contribute to the escalating prevalence of osteopenia osteoporosis. The application mechanical load through physical activity exercise prevents loss, while sufficient stimulates new acquisition. Osteocytes, cells embedded within bone, receive signals translate these cues into biological signals, termed mechano-transduction. Mechano-transduction regulate other resident cells, such as osteoblasts osteoclasts, orchestrate changes in mass. This review explores mechanisms which osteocyte-mediated response loading regulates osteoblast differentiation formation. An overview cell biology impact will be provided, emphasis on cues, mechano-transduction pathways, factors that direct progenitor toward lineage. While there are wide range clinically available treatments majority act manipulation osteoclast may have significant disadvantages. Despite central role deposition few therapies directly target preservation Improved understanding leading osteoblastogenesis reveal novel targets translational investigation.

Язык: Английский

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Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology

Frontiers in Molecular Biosciences, Год журнала: 2025, Номер 11

Опубликована: Янв. 13, 2025

Introduction Current in vitro intestinal models lack the mechanical forces present physiological environment, limiting their reliability for nanotoxicology studies. Here, we developed an enhanced Caco-2/HT29-MTX-E12 co-culture model incorporating orbital stimulation to better replicate conditions and investigate nanoparticle interactions. Methods We established co-cultures under static dynamic conditions, evaluating development through multiple approaches including barrier integrity measurements, gene expression analysis, confocal microscopy. introduced novel quantitative analysis of dome formation as a differentiation marker demonstrated application by investigating cellular responses titanium dioxide (TiO₂) nanoparticles digested food matrix. Results Dynamic accelerated epithelial differentiation, achieving functional properties day 14 rather than 21, with mucin production more organized three-dimensional structure. Mechanical selectively promoted goblet cell without affecting general markers. The optimized successfully detected concentration-dependent oxidative stress TiO₂ exposure, revealing dysfunction preceding membrane damage. Discussion This improved system provides platform By forces, each type exhibits representative behavior, creating realistic experimental setup. bridges gap between simple monocultures complex 3D systems, offering practical approach nanoparticle-epithelium interactions food-relevant context.

Язык: Английский

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Tanfloc-Modified Titanium Surfaces: Optimizing Blood Coagulant Activity and Stem Cell Compatibility

ACS Biomaterials Science & Engineering, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

This study explores the synergistic effects of combining titania nanotubes (TiNTs) with biopolymer Tanfloc (TAN) to enhance surface properties TiNTs for biomedical applications. We investigated interactions blood components and human adipose-derived stem cells (ADSCs) TiNT surfaces covalently functionalized (TAN), an aminolyzed polyphenolic tannin derivative. The (TiNT-TAN) have great potential control protein adsorption platelet adhesion activation. Fluorescence scanning electron microscopy (SEM) were used analyze adherence amphoteric nature multiple functional groups on TAN can adsorption, adhesion, Further, modified supports cell (ADSC) viability, attachment, growth without any cytotoxic effect. conjugation significantly (****p < 0.0001) increased proliferation rate ADSCs compared surfaces.

Язык: Английский

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Multi-index-dependent decellularization processes for scalable tissue-specific bioink production

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 161827 - 161827

Опубликована: Март 1, 2025

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Adjunctive therapies in orthodontics: a scoping systematic review

BMC Oral Health, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 18, 2025

Orthodontic tooth movement (OTM) induces physiological and sometimes pathological inflammation in periodontal tissues. This review evaluates the effectiveness of low-level laser therapy (LLLT), vibrational therapy, probiotics as adjunctive treatments for managing inflammation, pain, duration OTM. Medline via OVID, Cochrane, EMBASE, Web Science databases were utilized to identify randomized controlled trials (RCTs) published between January 1990 November 2023. Studies selected based on their evaluation LLLT, adjuncts fixed orthodontic treatment. LLLT shows promise enhancing by accelerating potentially reducing pain. However, disparate study outcomes indicate a need standardized application protocols. The efficacy an adjunct OTM remains inconclusive. Some studies this regard significant acceleration but most did not. Probiotic potential improve oral microbiota balance requires more rigorous determine its optimal administration methods. Future research should focus establishing guidelines protocols achieve consistent reliable across these therapies.

Язык: Английский

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Role of mechano-sensitive non-coding RNAs in bone remodeling of orthodontic tooth movement: recent advances

Progress in Orthodontics, Год журнала: 2022, Номер 23(1)

Опубликована: Дек. 30, 2022

Orthodontic tooth movement relies on bone remodeling and periodontal tissue regeneration in response to the complicated mechanical cues compressive tensive side. In general, stimulus regulates expression of mechano-sensitive coding non-coding genes, which turn affects how cells are involved remodeling. Growing numbers RNAs, particularly RNA, have been verified be essential for regulation osteogenesis osteoclastogenesis revealed they interact with signaling molecules do so. This review summarizes recent findings including microRNAs long as crucial regulators gene responding stimulation, outlines their roles deposition resorption. We focused multiple miRNAs such miR-21, - 29, -34, -103, -494-3p, -1246, -138-5p, -503-5p, -3198 that play a critical role function The emerging force-dependent lncRNAs also discussed extensively. summarized lncRNA XIST, H19, MALAT1 along other osteoclastogenesis. Ultimately, we look forward prospects novel application RNAs potential therapeutics regeneration.

Язык: Английский

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Fluid Shear Stress Regulates Osteogenic Differentiation via AnnexinA6-Mediated Autophagy in MC3T3-E1 Cells

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(24), С. 15702 - 15702

Опубликована: Дек. 11, 2022

Fluid shear stress (FSS) facilitates bone remodeling by regulating osteogenic differentiation, and extracellular matrix maturation mineralization. However, the underlying molecular mechanisms of how mechanical stimuli from FSS are converted into osteogenesis remain largely unexplored. Here, we exposed MC3T3-E1 cells to with different intensities (1 h 0, 5, 10, 20 dyn/cm2 intensities) treatment durations (10 0.5, 1, 2 4 treatment). The results demonstrate that 1 10 greatly upregulated expression markers (Runx2, ALP, Col I), accompanied AnxA6 activation. genetic ablation suppressed autophagic process, demonstrating lowered autophagy (Beclin1, ATG5, ATG7, LC3) decreased autophagosome formation, strongly reduced differentiation induced FSS. Furthermore, addition activator rapamycin knockdown stimulated coincided more expressions proteins ALP I under both static conditions. In conclusion, findings in this study reveal a hitherto unidentified relationship between FSS-induced autophagy, point as key mediator response FSS, which may provide new target for osteoporosis other diseases.

Язык: Английский

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Exploration of the miR‐187‐3p/CNR2 pathway in modulating osteoblast differentiation and treating postmenopausal osteoporosis through mechanical stress

The FASEB Journal, Год журнала: 2024, Номер 38(13)

Опубликована: Июль 3, 2024

Abstract This study aimed to explore how mechanical stress affects osteogenic differentiation via the miR‐187‐3p/CNR2 pathway. To conduct this study, 24 female C57BL/6 mice, aged 8 weeks, were used and divided into four groups. The Sham OVX groups did not undergo treadmill exercise, while + EX received a 8‐week exercise. Post‐training, bone marrow fresh femur samples collected for further analysis. Molecular biology analysis, histomorphology micro‐CT analysis conducted on these samples. Moreover, primary osteoblasts cultured under conditions GM group CTS group. cells in underwent sinusoidal stretching regimen either 3 or 7 days. expression of early osteoblast markers (Runx2, OPN, ALP) was measured assess differentiation. findings revealed that has regulatory impact miR‐187‐3p observed decrease, facilitating differentiation, CNR2 increased significantly. These observations suggest stress, miR‐187‐3p, play crucial roles regulating Both vivo vitro experiments have confirmed downregulates upregulates CNR2, which leads restoration distal femoral mass enhancement Therefore, promotes osteoblasts, resulting improved osteoporosis through signaling broad prospect provide molecular guidance basic research clinical application exercise prevention treatment PMOP.

Язык: Английский

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