Smart responsive in situ hydrogel systems applied in bone tissue engineering

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12

Опубликована: Май 28, 2024

The repair of irregular bone tissue suffers severe clinical problems due to the scarcity an appropriate therapeutic carrier that can match dynamic and complex damage. Fortunately, stimuli-responsive in situ hydrogel systems are triggered by a special microenvironment could be ideal method regenerating because injectability, gelatin, spatiotemporally tunable drug release. Herein, we introduce two main stimulus-response approaches, exogenous endogenous, forming hydrogels engineering. First, summarize specific distinct responses extensive range external stimuli (e.g., ultraviolet, near-infrared, ultrasound, etc.) form created from biocompatible materials modified various functional groups or hybrid nanoparticles. Furthermore, “smart” hydrogels, which respond endogenous physiological environmental temperature, pH, enzyme, etc.), achieve gelation one injection vivo without additional intervention. Moreover, mild chemistry response-mediated also offer fascinating prospects engineering, such as Diels–Alder, Michael addition, thiol-Michael Schiff reactions, etc. recent developments challenges smart their application administration engineering discussed this review. It is anticipated advanced strategies innovative ideas will exploited field increase quality life for patients with

Язык: Английский

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Mesenchymal Stem Cell-Derived Exosomes as a Treatment Option for Osteoarthritis

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9149 - 9149

Опубликована: Авг. 23, 2024

Osteoarthritis (OA) is a leading cause of pain and disability worldwide in elderly people. There critical need to develop novel therapeutic strategies that can effectively manage improve the quality life for older Mesenchymal stem cells (MSCs) have emerged as promising cell-based therapy age-related disorders due their multilineage differentiation strong paracrine effects. Notably, MSC-derived exosomes (MSC-Exos) gained significant attention because they recapitulate MSCs into benefits without causing any associated risks compared with direct cell transplantation. These help transport bioactive molecules such proteins, lipids, nucleic acids, which influence various cellular processes related tissue repair, regeneration, immune regulation. In this review, we provided an overview MSC-Exos considerable treatment option osteoarthritis. This review will go over underlying mechanisms by may alleviate pathological hallmarks OA, cartilage degradation, synovial inflammation, subchondral bone changes. Furthermore, summarized current preclinical evidence highlighted results from vitro vivo studies, well progress clinical trials using treat OA.

Язык: Английский

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Enhanced Osteogenic Differentiation for Bone Tissue Engineering: The Synergistic Effect of Adipose‐Derived Mesenchymal Stem Cell Exosomes and Carboxymethyl Chitosan Hydrogel

Polymers for Advanced Technologies, Год журнала: 2025, Номер 36(1)

Опубликована: Янв. 1, 2025

ABSTRACT Exosomes (EXOs) have emerged as critical mediators of intercellular communication with the potential to enhance bone regeneration through paracrine actions. The promising application EXOs in tissue engineering (TE) domains has recently sparked heightened research interest. selection type appropriate biomaterial, along method used produce a scaffold, is another factor determining successful TE. This study assessed impact incorporating adipose mesenchymal stem cell‐derived and its conditioned medium (CM) within chitosan/carboxymethyl chitosan (CTS/CMC2%) hydrogel on osteogenic differentiation. were characterized for their morphology, protein content, size using scanning electron microscope (SEM), bicinchoninic acid (BCA) assay, dynamic light scattering (DLS), Western blot. Adipose‐derived cells (ADMSCs) encapsulated hydrogels supplemented (12.5 25 μg/mL) or CM tested over 14 days. Cell viability, attachment, proliferation enhanced treatments versus controls confirmed by SEM, DAPI staining, MTT assay. Osteodifferentiation was evaluated alkaline phosphatase activity (ALP), Alizarin‐Red staining (ARS), calcium deposition, OSTEONECTIN immunostaining (ICC). According ICC results, ADMSCs CTS/CMC2% treated μg/mL (EXO25) exhibited 45.55% expression, highest percentage observed among all samples. Additionally, EXO25 high ALP expression after days ( p < 0.1). In conclusion, at different concentrations significantly impacted differentiation, particularly EXO25, cooperation designed (CTS/CMC2%), guiding cell fate specific targeting

Язык: Английский

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Quercetin-primed MSC exosomes synergistically attenuate osteoarthritis progression

Journal of Orthopaedic Surgery and Research, Год журнала: 2025, Номер 20(1)

Опубликована: Апрель 15, 2025

Osteoarthritis (OA), a degenerative joint disease characterized by cartilage degradation and inflammation, lacks effective disease-modifying therapies. Quercetin, bioactive flavonoid derived from Traditional Chinese Medicine, exhibits anti-inflammatory chondroprotective properties but is limited poor bioavailability. Mesenchymal stem cell-derived exosomes (MSC-Exos) offer promising strategy for targeted drug delivery regeneration. Bone marrow-derived MSC (Que-Exo) were isolated after preconditioning with quercetin (1µM, 24 h). Their effects evaluated in IL-1β-stimulated chondrocytes via RT-qPCR, Western blot, transcriptomics, proteomics. An ACLT-induced OA mouse model received intra-articular injections of Que-Exo, integrity assessed Safranin O staining OARSI scoring. Que-Exo significantly reduced IL-1β-induced pro-inflammatory markers (MMP9 COX-2) restored repair genes (SOX9 Collagen II) compared to untreated exosomes. Multi-omics analyses revealed activation PI3K-AKT signaling glutathione metabolism pathways. In vivo, mitigated preserved proteoglycan content. Quercetin-preconditioned synergistically enhance chondroprotection effects, offering novel therapeutic combining herbal compounds exosome-mediated delivery.

Язык: Английский

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Enhancing the Therapeutic Potential of Human Umbilical Cord Mesenchymal Stem Cells for Osteoarthritis: The Role of Platelet-Rich Plasma and Extracellular Vesicles

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3785 - 3785

Опубликована: Апрель 17, 2025

Osteoarthritis (OA) is a chronic degenerative joint disease. Our previous study demonstrated that extracellular vesicles (EVs) secreted by human umbilical cord mesenchymal stem cells (HUCMSCs), which play crucial role in regenerative medicine, have therapeutic effects on OA. Additionally, platelet-rich plasma (PRP) has been widely used musculoskeletal diseases as it promotes wound healing, angiogenesis, and tissue remodeling; however, its efficacy stand-alone therapy remains controversial. Therefore, we investigated the of combining cell-derived EVs with PRP an OA model. HUCMSC-derived treated were experimental group, whereas cultured serum-free (SF) or exosome-depleted fetal bovine serum (exo(-)FBS) served controls. PRP-treated HUCMSCs maintained their surface antigen characteristics potential to differentiate into adipocytes, osteoblasts, chondrocytes. In model, mice + 5% PRP-derived showed significantly improved motor function compared controls comparable those +SF +exo(-)FBS-derived EVs. increased type II collagen aggrecan decreased IL-1β expression observed cartilage transplanted various conclusion, enhances HUCMSC differentiation, treatment improves outcomes, providing promising strategy for future clinical applications.

Язык: Английский

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Cartilage Repair: Promise of Adhesive Orthopedic Hydrogels

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 9984 - 9984

Опубликована: Сен. 16, 2024

Cartilage repair remains a major challenge in human orthopedic medicine, necessitating the application of innovative strategies to overcome existing technical and clinical limitations. Adhesive hydrogels have emerged as promising candidates for cartilage promotion tissue engineering, offering key advantages such enhanced integration therapeutic potential. This comprehensive review navigates landscape adhesive repair, discussing identified challenges, shortcomings current treatment options, unique hydrogel products scaffolds. While emphasizing critical need situ lateral with surrounding tissues, we dissect limitations outline future perspectives scaffolds repair. Moreover, examine translation pathway regulatory considerations specific hydrogels. Overall, this synthesizes insights knowledge gaps highlights directions research regarding hydrogel-based devices advancing engineering.

Язык: Английский

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Glycosphingolipids in Osteoarthritis and Cartilage Regeneration Therapy: Mechanisms and Therapeutic Prospects

Опубликована: Март 6, 2024

Glycosphingolipids (GSLs), a subtype of glycolipids containing sphingosine, are critical components vertebrate plasma membranes, playing pivotal role in cellular signaling and interactions. In human articular cartilage osteoarthritis (OA), GSL expression is known notably to decrease. This review focuses on the roles gangliosides, specific type GSL, degeneration regeneration, emphasizing their regulatory function signal transduction. The whether endogenous or augmented exogenously, regulated at enzymatic level, targeting glycosyltransferases. regulation has significant implications for composition cell surface gangliosides impact transduction chondrocytes progenitor cells. Different levels ganglioside can influence pathways various ways, potentially affecting properties, including malignancy. Moreover, gene manipulations against have been shown regulate metabolism chondrocyte differentiation vivo vitro. highlights potential development therapeutic strategies injury addresses promising directions future research treatment.

Язык: Английский

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Platelet Concentrates Preconditioning of Mesenchymal Stem Cells and Combined Therapies: Integrating Regenerative Strategies for Enhanced Clinical Applications

Cell Transplantation, Год журнала: 2024, Номер 33

Опубликована: Янв. 1, 2024

This article presents a comprehensive review of the factors influencing efficacy mesenchymal stem cells (MSCs) transplantation and its association with platelet concentrates (PCs). It focuses on investigating impact PCs’ composition, age health status donors, application methods, environmental outcomes relevant treatments. In addition, it delves into strategies mechanisms for optimizing MSCs PCs, encompassing preconditioning combined therapies. Furthermore, provides an in-depth exploration signaling pathways proteomic characteristics associated emphasizes specific effects therapy. The also introduces latest advancements in biomaterials regenerative medical strategies, stimulating scholarly discourse this subject. Through review, primary goal is to facilitate more profound comprehension treatment outcomes, as well medicine, offering theoretical guidance practical references related research clinical practice.

Язык: Английский

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Investigating the Differential Therapeutic Efficacy and Mechanisms of Human Umbilical Cord Mesenchymal Stem Cells at Various Passages in Osteoarthritis Treatment

Tissue and Cell, Год журнала: 2024, Номер 90, С. 102499 - 102499

Опубликована: Июль 31, 2024

This study aimed to assess the clinical efficacy of umbilical cord mesenchymal stem cells (hUC-MSCs) from different passages (P3, P8, and P13) in treatment knee osteoarthritis (OA) explore underlying mechanisms. The hUC-MSCs each passage were characterized evaluated for their stemness, migration, proliferation, marker expression. Rats with OA treated passage, therapeutic effects assessed based on swelling, discomfort, pathological examination joint. Co-culture experiments conducted examine ability stimulate type II collagen synthesis inhibit MMP13 expression chondrocytes. Telomere length telomerase activity measured investigate reasons observed differences efficacy. results revealed that P3 P8 exhibited superior osteogenic chondrogenic differentiation potential compared P13, while P13 demonstrated stronger adipogenic differentiation. wound healing rate was significantly higher groups P13. All hUC-MSC expressed high levels CD90 CD105, indicating cell characteristics, CD31 CD45 not expressed. CD105 reduced group. In rat osteoarthritis, there no significant Mankin scores, findings between treatments. However, a difference 8th 13th passages. showed enhanced Although groups, found groups. analysis samples had shorter telomeres both P8. positive hUC-MSCs, these passages, it negative hUC-MSCs. conclusion, possibly due capacity activity.

Язык: Английский

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A Macroscopic Exploration of the Ideoscape on Exosomes for Bone Regeneration

Osteology, Год журнала: 2024, Номер 4(4), С. 159 - 178

Опубликована: Окт. 8, 2024

Background: Exosomes, nanoscale extracellular vesicles, play a crucial role in tissue physiology and regeneration. This study uses infometric techniques to explore the structure of exosome-based bone regeneration research. Methods: We applied BERTopic, an advanced topic modeling algorithm, comprehensive corpus scientific literature on exosomes regeneration, identifying key themes such as stem cell studies, healing, regenerative applications, with orthopedics dentistry emerging dominant subfields. To further investigate ‘ideoscape’, i.e., conceptual landscape that maps how ideas, methods, are interconnected across field, we extracted significant concepts from abstracts using GPT 3.5 turbo created knowledge graphs. Results: Our analysis revealed rapid growth field dental which has outpaced other topics by twofold. highlighted central periodontal cells their cellular processes—proliferation, migration, differentiation—along clinical applications. approach provided clear visualization field’s intellectual structure, showing interconnected. findings offer view evolving trends revealing not only most active research areas but also gaps opportunities for investigation. Conclusions: exemplifies utility combining graph creation map trends, offering flexible largely automated tool researchers vast bodies guide future directions.

Язык: Английский

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