Insectes Sociaux, Год журнала: 2023, Номер 71(1), С. 17 - 27
Опубликована: Ноя. 22, 2023
Язык: Английский
ACS Applied Bio Materials, Год журнала: 2025, Номер unknown
Опубликована: Фев. 28, 2025
Patients and healthcare systems face significant social financial challenges due to the increasing number of individuals with chronic external internal wounds that fail heal. The complexity healing process remains a serious health concern, despite effectiveness conventional wound dressings in promoting healing. Recent advancements materials science fabrication techniques have led development innovative enhance To further expedite process, novel approaches such as nanoparticles, 3D-printed dressings, biomolecule-infused emerged, along cell-based methods. Additionally, gene therapy technologies are being harnessed generate stem cell derivatives more functional, selective, responsive than their natural counterparts. This review highlights potential biomaterials, 3D bioprinting, gene- therapies However, it also underscores necessity for research address existing integrate these strategies into standard clinical practice.
Язык: Английский
Процитировано
3
Nature Cell Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 9, 2025
Язык: Английский
Процитировано
1
Nature Communications, Год журнала: 2022, Номер 13(1)
Опубликована: Ноя. 17, 2022
Abstract The thyroid gland captures iodide in order to synthesize hormones that act on almost all tissues and are essential for normal growth metabolism. Low plasma levels of lead hypothyroidism, which is one the most common disorder humans not always satisfactorily treated by lifelong hormone replacement. Therefore, addition lack vitro tractable models study human development, differentiation maturation, functional organoids could pave way explore new therapeutic approaches. Here we report generation transplantable derived from embryonic stem cells capable restoring athyreotic mice as a proof concept future development.
Язык: Английский
Процитировано
37
Global Change Biology, Год журнала: 2021, Номер 28(1), С. 54 - 71
Опубликована: Окт. 20, 2021
Rapid climate change is placing many marine species at risk of local extinction. Recent studies show that epigenetic mechanisms (e.g. DNA methylation, histone modifications) can facilitate both within and transgenerational plasticity to cope with changing environments. However, reprogramming (erasure re-establishment marks) during gamete early embryo development may hinder inheritance. Most our knowledge about stems from mammals model organisms, whereas the prevalence extent among non-model wild populations rarely investigated. Moreover, whether dynamics are sensitive environmental conditions not well known, representing a key gap in pursuit identify underlying links between parental exposure patterns environmentally adapted offspring phenotypes. Here, we investigated (DNA methylation/hydroxymethylation) gene expression across gametogenesis embryogenesis stickleback (Gasterosteus aculeatus) under three ocean warming scenarios (ambient, +1.5 +4°C). We found acclimation led dynamic temperature-sensitive throughout development. Both global methylation/hydroxymethylation genes involved modifications were strongly differentially affected by increased scenarios. Comparing transcriptomic profiles gonads, mature gametes embryonic stages showed sex-specific accumulation temperature sensitivity several actors. methyltransferase induction was primarily maternally inherited (suggesting maternal control remethylation), histone-modifying enzymes shaped parents. Importantly, massive, temperature-specific changes landscape occurred blastula, critical stage for successful development, which could, thus, translate substantial consequences phenotype resilience In summary, study identified regulation, reflecting potential 'windows opportunity' adaptive responses future change.
Язык: Английский
Процитировано
40
Nucleic Acids Research, Год журнала: 2021, Номер 49(16), С. 9132 - 9153
Опубликована: Авг. 3, 2021
Transposable elements (TEs) occupy nearly 40% of mammalian genomes and, whilst most are fragmentary and no longer capable transposition, they can nevertheless contribute to cell function. TEs within genes transcribed by RNA polymerase II be copied as parts primary transcripts; however, their full contribution mature transcript sequences remains unresolved. Here, using long short read (LR SR) sequencing data, we show that 26% coding 65% noncoding transcripts in human pluripotent stem cells (hPSCs) contain TE-derived sequences. Different TE families incorporated into RNAs unique patterns, with consequences structure The presence a is correlated TE-type specific changes its subcellular distribution, alterations steady-state levels half-life, differential association Binding Proteins (RBPs). We identify hPSC-specific incorporation endogenous retroviruses (ERVs) LINE:L1 protein-coding mRNAs, which generate sequence-derived peptides. Finally, single RNA-seq reveals hPSCs express ERV-containing transcripts, differentiating subpopulations lack ERVs SINE LINE-containing transcripts. Overall, our comprehensive analysis demonstrates the more widespread has greater impact than previously appreciated.
Язык: Английский
Процитировано
35
Frontiers in Cell and Developmental Biology, Год журнала: 2022, Номер 9
Опубликована: Янв. 11, 2022
Wound healing is one of the most complex physiological regulation mechanisms human body. Stem cell technology has had a significant impact on regenerative medicine. Adipose stem cells (ASCs) have many advantages, including their ease harvesting and high yield, rich content components cytokines, strong practicability. They rapidly become favored tool in Here, we summarize mechanism clinical therapeutic potential ASCs wound repair.
Язык: Английский
Процитировано
28
Animal Reproduction Science, Год журнала: 2023, Номер 256, С. 107321 - 107321
Опубликована: Авг. 19, 2023
Язык: Английский
Процитировано
16
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(3), С. 2658 - 2658
Опубликована: Янв. 31, 2023
Complex functioning of the genome in cell nucleus is controlled at different levels: (a) DNA base sequence containing all relevant inherited information; (b) epigenetic pathways consisting protein interactions and feedback loops; (c) architecture organization activating or suppressing genetic between parts genome. Most research so far has shed light on puzzle pieces these levels. This article, however, attempts an integrative approach to expression regulation incorporating layers. Under environmental stress during development, differentiation towards specialized types, dysfunctional tumor, seems react as a whole through coordinated changes levels control. implies need for framework which biological, chemical, physical manifestations can serve basis coherent theory gene self-organization. An international symposium held Biomedical Research Study Center Riga, Latvia, 25 July 2022 addressed novel aspects abovementioned topic. The present article reviews most recent results conclusions state-of-the-art this multidisciplinary field science, were delivered discussed by scholars Riga symposium.
Язык: Английский
Процитировано
15
eLife, Год журнала: 2024, Номер 13
Опубликована: Май 30, 2024
Germline epigenetic programming, including genomic imprinting, substantially influences offspring development. Polycomb Repressive Complex 2 (PRC2) plays an important role in Histone 3 Lysine 27 trimethylation (H3K27me3)-dependent loss of which leads to growth and developmental changes mouse offspring. In this study, we show that from oocytes lacking the PRC2 protein Embryonic Ectoderm Development (EED) were initially developmentally delayed, characterised by low blastocyst cell counts substantial delay mid-gestation embryos. This initial was resolved as underwent accelerated fetal development late gestation resulting similar stage weight controls at birth. The Eed -null associated with remodelling placenta, involved increase maternal tissue size, conspicuous expansion glycogen-enriched population, delayed parturition. Despite placental development, efficiency, blood glucose levels low, metabolome unchanged. Moreover, while expression H3K27me3-imprinted gene amino acid transporter Slc38a4 increased, individual acids controls, indicating transport not enhanced. Genome-wide analyses identified extensive transcriptional dysregulation DNA methylation affected placentas, a range imprinted non-imprinted genes. Together, deletion growing resulted hyperplasia, our data indicate remarkable capacity for be normalised despite inefficient function H3K27me3-dependent imprinting.
Язык: Английский
Процитировано
5
Cells, Год журнала: 2022, Номер 11(5), С. 863 - 863
Опубликована: Март 2, 2022
Somatic embryogenesis (SE), which is a process that involves the in vitro-induced embryogenic reprogramming of plant somatic cells, requires dynamic changes cell transcriptome. These are fine-tuned by many genetic and epigenetic factors, including posttranslational histone modifications such as acetylation. Antagonistically acting enzymes, acetyltransferases (HATs) deacetylases (HDACs), control acetylation developmental processes, believed to SE. However, function specific HAT/HDACs genes subjected acetylation-mediated regulation during SE have yet be revealed. Here, we present global gene-specific Arabidopsis explants undergoing In TSA (trichostatin A)-induced SE, demonstrate H3 H4 might expression critical transcription factor (TF) vital role LEC1, LEC2 (LEAFY COTYLEDON 1; 2), FUS3 (FUSCA 3) MYB118 (MYB DOMAIN PROTEIN 118). Within HATs HDACs, mainly positively regulate identified HDA19 negatively affecting regulating BBM. Finally, provide some evidence on Our results reveal an essential mechanisms TF play roles cells. The implicate complexity Hac-related gene induction point differences regulatory involved auxin- TSA-induced
Язык: Английский
Процитировано
19