Applied Microbiology and Biotechnology,
Год журнала:
2023,
Номер
107(14), С. 4429 - 4445
Опубликована: Май 29, 2023
Abstract
Stem
cell–based
cell
therapeutics
and
especially
those
based
on
human
mesenchymal
stem
cells
(hMSCs)
induced
pluripotent
(hiPSCs)
are
said
to
have
enormous
developmental
potential
in
the
coming
years.
Their
applications
range
from
treatment
of
orthopedic
disorders
cardiovascular
diseases
autoimmune
even
cancer.
However,
while
more
than
27
hMSC-derived
currently
commercially
available,
hiPSC-based
yet
complete
regulatory
approval
process.
Based
a
review
current
available
therapeutic
products
upcoming
hiPSC-derived
phase
2
3,
this
paper
compares
therapy
manufacturing
process
between
these
two
types.
Moreover,
similarities
as
well
differences
highlighted
resulting
impact
production
discussed.
Here,
emphasis
is
placed
(i)
hMSC
hiPSC
characteristics,
safety,
ethical
aspects,
(ii)
their
morphology
requirements,
(iii)
2-
3-dimensional
cultivations
dependence
applied
culture
medium
mode.
In
doing
so,
also
downstream
processing
aspects
covered
role
single-use
technology
Key
points
•
Mesenchymal
exhibit
distinct
behaviors
during
cultivation
Single-use
stirred
bioreactor
systems
preferred
for
both
types
Future
research
should
adapt
modify
processes
devices
Multipotent
mesenchymal
stem
cells
(MSCs)
have
high
self-renewal
and
multi-lineage
differentiation
potentials
low
immunogenicity,
so
they
attracted
much
attention
in
the
field
of
regenerative
medicine
a
promising
clinical
application.
MSCs
originate
from
mesoderm
can
differentiate
not
only
into
osteoblasts,
cartilage,
adipocytes,
muscle
but
also
ectodermal
endodermal
cell
lineages
across
embryonic
layers.
To
design
therapy
for
replacement
damaged
tissues,
it
is
essential
to
understand
signaling
pathways,
which
major
impact
on
MSC
differentiation,
as
this
will
help
integrate
inputs
initiate
specific
lineage.
Hedgehog
(Hh)
plays
vital
role
development
various
tissues
organs
embryo.
As
morphogen,
Hh
regulates
survival
proliferation
tissue
progenitor
populations
critical
moderator
involving
tri-lineage
layer
MSCs.
This
review
summarizes
pathway
mesodermal,
endodermal,
cells.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(13), С. 6973 - 6973
Опубликована: Июнь 26, 2024
The
risks
of
severe
ionizing
radiation
exposure
are
increasing
due
to
the
involvement
nuclear
powers
in
combat
operations,
use
power,
and
existence
terrorist
threats.
Exposure
a
whole-body
dose
above
about
0.7
Gy
results
H-ARS
(hematopoietic
acute
syndrome),
which
is
characterized
by
damage
hematopoietic
system;
higher
doses
result
further
gastrointestinal
nervous
systems.
Only
few
medical
countermeasures
for
ARS
currently
available
approved
use,
although
others
development.
Cell
therapies
(cells
or
products
produced
cells)
complex
therapeutics
that
show
promise
treatment
injury
have
been
shown
reduce
mortality
morbidity
animal
models.
Since
clinical
trials
cannot
be
ethically
conducted,
testing
extremely
important.
Here,
we
describe
cell
tested
Both
cells
appear
promote
survival
lessen
tissue
after
irradiation,
mechanisms
not
clear.
Because
often
occurs
conjunction
with
other
traumatic
injuries,
models
combined
involving
future
countermeasure
these
problems
also
discussed.
Frontiers in Cell and Developmental Biology,
Год журнала:
2023,
Номер
11
Опубликована: Окт. 23, 2023
Osteoimmunology
is
a
concept
involving
molecular
and
cellular
crosstalk
between
the
skeletal
immune
systems.
Toll-like
receptors
(TLRs)
are
widely
expressed
both
on
mesenchymal
stromal
cells
(MSCs),
hematopoietic
cells,
in
osteogenic
microenvironment
for
bone
development
or
repair.
TLRs
can
sense
exogenous
pathogen-associated
patterns
(PAMPs)
derived
from
microorganisms,
damage-associated
(DAMPs)
normal
subjected
to
injury,
inflammation,
cell
apoptosis
under
physiological
pathological
conditions.
Emerging
studies
reported
that
TLR
signaling
plays
an
important
role
remodeling
by
directly
impacting
MSC
differentiation
osteoimmunology.
However,
how
regulate
critical
remains
be
elucidated
promote
of
MSCs
new
formation
tissue
This
review
outlines
distinct
variants
various
tissues,
detailing
impact
pathway
activation
inhibition
differentiation.
It
also
elucidates
pathways’
interplay
with
osteoclasts,
extracellular
vesicles
(EVs)
MSCs.
Furthermore,
we
explore
biomaterial-based
guide
MSCs’
Therefore,
understanding
TLRs’
this
context
has
significant
implications
advancing
regeneration
repair
strategies.
Applied Microbiology and Biotechnology,
Год журнала:
2023,
Номер
107(14), С. 4429 - 4445
Опубликована: Май 29, 2023
Abstract
Stem
cell–based
cell
therapeutics
and
especially
those
based
on
human
mesenchymal
stem
cells
(hMSCs)
induced
pluripotent
(hiPSCs)
are
said
to
have
enormous
developmental
potential
in
the
coming
years.
Their
applications
range
from
treatment
of
orthopedic
disorders
cardiovascular
diseases
autoimmune
even
cancer.
However,
while
more
than
27
hMSC-derived
currently
commercially
available,
hiPSC-based
yet
complete
regulatory
approval
process.
Based
a
review
current
available
therapeutic
products
upcoming
hiPSC-derived
phase
2
3,
this
paper
compares
therapy
manufacturing
process
between
these
two
types.
Moreover,
similarities
as
well
differences
highlighted
resulting
impact
production
discussed.
Here,
emphasis
is
placed
(i)
hMSC
hiPSC
characteristics,
safety,
ethical
aspects,
(ii)
their
morphology
requirements,
(iii)
2-
3-dimensional
cultivations
dependence
applied
culture
medium
mode.
In
doing
so,
also
downstream
processing
aspects
covered
role
single-use
technology
Key
points
•
Mesenchymal
exhibit
distinct
behaviors
during
cultivation
Single-use
stirred
bioreactor
systems
preferred
for
both
types
Future
research
should
adapt
modify
processes
devices
