Novel multiparametric bulk and single EV pipeline for adipose cell-specific biomarker discovery in paired human biospecimens DOI Open Access
Paola Loreto Palacio, Jacelyn Greenwald, Kim Truc Nguyen

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 19, 2024

Abstract Obesity is a global health crisis that contributes to morbidity and mortality worldwide. Obesity’s comorbid association with variety of diseases, from metabolic syndrome neurodegenerative disease, underscores the critical need better understand pathobiology obesity. Adipose tissue, once seen as an inert storage depot, now recognized active endocrine organ, regulating systemic homeostasis. Recent studies spotlight theranostic utility extracellular vesicles (EVs) novel biomarkers drivers including obesity-related complications. Adipose-derived EVs (ADEVs) have garnered increased interest for their roles in diverse however robust isolation characterization protocols human, cell-specific EV subsets are limited. Herein, we directly address this technical challenge by establishing multiparametric analysis framework leverages bulk single characterization, mRNA phenotyping proteomics human ADEVs paired visceral adipose cultured mature adipocyte conditioned media, plasma obese subjects undergoing bariatric surgery. Importantly, rigorous at tissue level identified top ‘adipose liquid biopsy’ candidates were validated circulating same patient. In summary, our study paves way toward cell-specific, studying obesity-driven disease.

Язык: Английский

White adipocyte dysfunction and obesity-associated pathologies in humans DOI
Carolina E. Hagberg, Kirsty L. Spalding

Nature Reviews Molecular Cell Biology, Год журнала: 2023, Номер 25(4), С. 270 - 289

Опубликована: Дек. 12, 2023

Язык: Английский

Процитировано

62

Gut microbiota in overweight and obesity: crosstalk with adipose tissue DOI
Patrice D. Cani, Matthias Van Hul

Nature Reviews Gastroenterology & Hepatology, Год журнала: 2023, Номер 21(3), С. 164 - 183

Опубликована: Дек. 8, 2023

Язык: Английский

Процитировано

57

The evolving functions of the vasculature in regulating adipose tissue biology in health and obesity DOI
Ibrahim AlZaim, Laura de Rooij, Bilal N. Sheikh

и другие.

Nature Reviews Endocrinology, Год журнала: 2023, Номер 19(12), С. 691 - 707

Опубликована: Сен. 25, 2023

Язык: Английский

Процитировано

47

Adipose organ dysfunction and type 2 diabetes: Role of nitric oxide DOI
Zahra Bahadoran, Parvin Mirmiran, Asghar Ghasemi

и другие.

Biochemical Pharmacology, Год журнала: 2024, Номер 221, С. 116043 - 116043

Опубликована: Фев. 5, 2024

Язык: Английский

Процитировано

8

Translational potential of mouse models of human metabolic disease DOI Creative Commons
I. Sadaf Farooqi, Yong Xu

Cell, Год журнала: 2024, Номер 187(16), С. 4129 - 4143

Опубликована: Июль 26, 2024

Obesity causes significant morbidity and mortality globally. Research in the last three decades has delivered a step-change our understanding of fundamental mechanisms that regulate energy homeostasis, building on foundational discoveries mouse models metabolic disease. However, not all findings made rodents have translated to humans, hampering drug discovery this field. Here, we review how studies mice humans informed current framework for discuss their challenges limitations, offer perspective human may play an increasingly important role disease identification therapeutic targets future.

Язык: Английский

Процитировано

8

The challenges of assessing adiposity in a clinical setting DOI
Emma Börgeson, Saeideh Tavajoh, Stephan Lange

и другие.

Nature Reviews Endocrinology, Год журнала: 2024, Номер 20(10), С. 615 - 626

Опубликована: Июль 15, 2024

Язык: Английский

Процитировано

7

Emerging debates and resolutions in brown adipose tissue research DOI
Aaron M. Cypess, Barbara Cannon, Jan Nedergaard

и другие.

Cell Metabolism, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

7

Activin E is a new guardian protecting against hepatic steatosis via inhibiting lipolysis in white adipose tissue DOI Creative Commons

Shi‐Young Park,

Yoonil Cho,

Sung Ok Son

и другие.

Experimental & Molecular Medicine, Год журнала: 2025, Номер unknown

Опубликована: Фев. 13, 2025

Abstract Hepatic endoplasmic reticulum (ER) stress is implicated in the development of steatosis and its progression to nonalcoholic steatohepatitis (NASH). The ER liver can sustain metabolic function by activating defense mechanisms that delay or prevent fatty disease (NAFLD). However, precise which response protects against NAFLD remain largely unknown. Recently, activin E has been linked diseases such as insulin resistance NAFLD. physiological conditions regulatory driving hepatic Inhbe expression (which encodes E) well role require further investigation. Here we found increased under prolonged fasting conditions, was mediated ATF4, determined promoter analysis a mouse model. Consistently, positive correlation between INHBE ATF4 levels relation status confirmed using public human datasets. To investigate steatosis, assessed fluxes lipid metabolism an -knockout These mice displayed lean phenotype but developed severe high-fat diet. deficiency resulted lipolysis adipose tissue, leading acid influx into liver. Conversely, overexpression ameliorated suppressing tissue through ALK7–Smad signaling. In conclusion, serves hepatokine prevents liver, thereby protecting

Язык: Английский

Процитировано

1

Overfeeding induces adipose tissue release of distinct mitochondria DOI Creative Commons
Joshua Goodman, Chloé Berland, Rajesh K. Soni

и другие.

Cell Reports, Год журнала: 2025, Номер 44(2), С. 115318 - 115318

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1

In Vitro Modeling of Interorgan Crosstalk: Multi-Organ-on-a-Chip for Studying Cardiovascular-Kidney-Metabolic Syndrome DOI
Cody Juguilon, Ramak Khosravi, Milica Radisic

и другие.

Circulation Research, Год журнала: 2025, Номер 136(11), С. 1476 - 1493

Опубликована: Май 22, 2025

Cardiovascular-kidney-metabolic syndrome is a progressive disorder driven by perturbed interorgan crosstalk among adipose, liver, kidney, and heart, leading to multiorgan dysfunction. Capturing the complexity of human cardiovascular-kidney-metabolic pathophysiology using conventional models has been challenging. Multi-organ-on-a-chip platforms offer versatile means study underlying signaling at different stages bolster clinical translation.

Язык: Английский

Процитировано

1