Biohybrid neural interfaces: improving the biological integration of neural implants

Chemical Communications, Год журнала: 2023, Номер 59(100), С. 14745 - 14758

Опубликована: Янв. 1, 2023

Biohybrid design enables the engineering of neural interfaces with enhanced functionality and performance.

Язык: Английский

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Organoids in concert: engineering in vitro models toward enhanced fidelity

Aggregate, Год журнала: 2024, Номер 5(2)

Опубликована: Янв. 4, 2024

Abstract Organoids have emerged as a powerful platform for studying complex biological processes and diseases in vitro. However, most studies focused on individual organoids, overlooking the inter‐organ interactions vivo limiting physiological relevance of models. To address this limitation, development multi‐organoid system has gained considerable attention. This aims to recapitulate communication enable study processes. review provides comprehensive overview recent advancements organoid engineering emerging strategies constructing system. First, we highlight critical mechanical, structural, biochemical factors involved designing suitable materials growth different organoids. Additionally, discuss incorporation dynamic culture environments enhance inter‐organoid communication. Furthermore, explore techniques manipulating morphogenesis spatial positioning organoids establish effective networks. We summarize achievements utilizing vitro, including assembloids microfluidic platforms. Lastly, existing challenges opportunities developing from its technical bottlenecks scalability applications toward human diseases.

Язык: Английский

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Bioelectric stimulation outperforms brain derived neurotrophic factor in promoting neuronal maturation

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 8, 2025

Neuronal differentiation and maturation are crucial for developing research models therapeutic applications. Brain-derived neurotrophic factor (BDNF) is a widely used biochemical stimulus promoting neuronal maturation. However, the broad effects of stimuli on multiple cellular functions limit their applicability in both vitro clinical settings. Electrical stimulation (ES) offers promising physical method to control cell fate function, but it hampered by lack standard optimised protocols. In this study, we demonstrate that ES outperforms BDNF human neuroblastoma SH-SY5Y. Additionally, address question regarding which parameters regulate biological responses. The SH-SY5Y cells were tested under several pulsed regimes. We identified accumulated charge effective electric field time as novel criteria determining optimal obtained using electrochemical characterisation equivalent circuit modelling. Our findings show correlates with amount during ES. Higher accumulation (~ 50 mC/h) significantly promotes extensive neurite outgrowth ramification, enhances expression synaptophysin, yielding exceeding those BDNF. contrast, fewer injection culture 0.1 minimally induces increases proliferation. Moreover, altered concentration protein cargo secreted extracellular vesicles (EV). large enough enriched EV proteome associated neural development function. These results each regime distinct Increased facilitates complex morphologies axonal outperforming exogenous factors. Controlled methods immediately applicable creating mature cultures minimal chemical intervention.

Язык: Английский

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The Sentient Cell

Oxford University Press eBooks, Год журнала: 2023, Номер unknown

Опубликована: Окт. 5, 2023

Abstract This volume is the natural follow-up to Arthur Reber’s 2019 book, The First Minds: Caterpillars, ‘Karyotes, and Consciousness (TFM). In that earlier work, Cellular Basis of (CBC) theory was developed based on a number efforts published in variety journals between 1997 as well talks, colloquia, presentations at conferences. core proposition TFM life mind are co-terminous. All organisms, all species extant extinct, sentient. have an existentially secure consciousness—without which they would been evolutionary dead-ends, unable survive chaotic, dangerous environment first appeared. And, importantly, forms sentience, cognitive functioning right up including those expressed by humans, evolved from original expression consciousness birth prokaryotes. unicellular widely accepted, foundational principle biological social sciences. CBC simply applies same sentience.

Язык: Английский

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Cyborg islets: implanted flexible electronics reveal principles of human islet electrical maturation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 20, 2024

Flexible electronics implanted during tissue formation enable chronic studies of tissue-wide electrophysiology. Here, we integrate tissue-like stretchable organogenesis human stem cell-derived pancreatic islets, stably tracing single-cell extracellular spike bursting dynamics over months functional maturation. Adapting sorting methods from neural reveals maturation-dependent electrical patterns α and β-like (SC-α β) cells, their stimulus-coupled dynamics. We identified two major states for both SC-α β distinguished by glucose threshold action potential firing. find that improved hormone stimulation capacity extended culture reflects increasing numbers SC-α/β cells in low basal firing states, linked to energy metabolism gene upregulation. Continuous recording further maturation entrainment daily feeding cycles circadian islet-level secretion rhythms reflect sustained coordinate oscillation cell-level activities. this correlates with cell-cell communication exocytic network induction, indicating a role coordinating system-level responses. Cyborg islets thus reveal principles will be useful build fully

Язык: Английский

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Brain organoid maturation and implantation integration based on electrical signals input

Journal of Advanced Research, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

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Multi-layered electrode constructs for neural tissue engineering

Journal of Materials Chemistry B, Год журнала: 2025, Номер 13(10), С. 3390 - 3404

Опубликована: Янв. 1, 2025

Although neural tissue engineering holds great therapeutic potential for multiple clinical applications, one important challenge is the development of scaffolds that provide cues required development. To achieve this, biomaterial systems can be leveraged to present appropriate biological, mechanical, topographical and electrical could direct cell fate. In this study, a multi-layered electrode construct was engineered used as platform 3D encapsulation in vitro applications. The first layer conductive hydrogel coating, improves conductivity from underlying platinum electrode. second biosynthetic hydrogel, specifically tailored support This layered electrochemically characterised, numerical model applied study stimuli reaching layer. shown effectively growth proliferation encapsulated astrocytes within layer, while will enable computational experimentation benchmarking validation. highly versatile system represents robust tool influence on fate, well investigating biohybrid interfaces vitro.

Язык: Английский

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Organoids – the future of pre-clinical development of AAV gene therapy for CNS disorders

Gene Therapy, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

Abstract Advancements in our understanding of genetic disease and adeno-associated virus has prompted great excitement into the field AAV-mediated gene therapy, particularly for diseases central nervous system, including retinal disorders. Despite significant progress, exemplified by approval therapies such as Luxturna® Zolgensma®, a substantial number remain pre-clinical or early clinical stages, with many failing to advance later phases. Whilst use animal models test safety delivery route efficacy AAV treatments is imperative, differences tissue structure physiology between humans restricted precise modelling therapy development CNS Alongside FDA push non-animal alternative models, researchers are increasingly turning human-based stem cell-derived organoids, which can offer more accurate representation human cellular microenvironments niches. As such, this review explores advantages limitations brain organoids disease, primary focus on their utility identifying novel capsids, cell-specific promoters, role recent studies.

Язык: Английский

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Bioelectric and Physicochemical Foundations of Bioelectronics in Tissue Regeneration

Biomaterials, Год журнала: 2025, Номер unknown, С. 123385 - 123385

Опубликована: Май 1, 2025

Язык: Английский

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Musculoskeletal Organoids: An Emerging Toolkit for Establishing Personalized Models of Musculoskeletal Disorders and Developing Regenerative Therapies

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Musculoskeletal (MSK) conditions are the primary cause of physical disability globally. These disorders physically and mentally debilitating severely impact patients' quality life. As median age world's population increases, there has been an intensifying urgency developing efficacious therapies for various orthopaedic conditions. Furthermore, highly heterogeneous nature MSK calls a personalized approach to studying disease mechanisms regenerative treatments. Organoids have emerged as advanced generating functional tissue/organ mimics in vitro, which hold promise regeneration, modeling, therapeutic development. Herein, we review preparation, characterization, application organoids. We highlight potential patient-specific organoids development medicine discuss challenges opportunities future STATEMENT OF SIGNIFICANCE: Despite decades research, translation research into clinical applications remains limited, partially attributed our inadequate understanding mechanisms. To advance development, critical needs models with higher relevance predictive power. Additionally, engineered constructs that closely mimic structural features native tissues desirable. promising meet above requirements. unleash full necessitates comprehensive their categories, construction, functions, applications, challenges. This aims fulfill this crucial need, aiming accelerate organoid platforms benefit millions patients afflicted

Язык: Английский

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