Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 399 - 449
Опубликована: Ноя. 29, 2024
Язык: Английский
Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 399 - 449
Опубликована: Ноя. 29, 2024
Язык: Английский
Cell Reports Methods, Год журнала: 2025, Номер unknown, С. 101028 - 101028
Опубликована: Апрель 1, 2025
Multi-cell-type, 3D microphysiological systems (MPS) that recapitulate normal organ/organ system functions and the progression of diseases are being applied in drug discovery development programs to enable precision medicine. A critical step for this application is demonstrate reproducibility MPS its ability identify biologic/clinical heterogeneity from experimental variability, which requires capturing detailed metadata associated with studies as well a strong analytical approach assessing reproducibility. Detailed ensure identical study parameters compared when evaluating We have developed Pittsburgh protocol (PReP), uses set common statistical metrics, coefficient variation (CV), ANOVA, intraclass correlation (ICC), pipeline standard evaluate intra- interstudy performance. The PReP can be employed biological/clinical relevant
Язык: Английский
Процитировано
0Stem Cell Reviews and Reports, Год журнала: 2025, Номер unknown
Опубликована: Май 19, 2025
Язык: Английский
Процитировано
0Frontiers in Public Health, Год журнала: 2024, Номер 12
Опубликована: Июль 22, 2024
In the past decade, significant European calls for research proposals have supported translational collaborative on non-communicable and infectious diseases within biomedical life sciences by bringing together interdisciplinary multinational consortia. This has advanced our understanding of disease pathophysiology, marking considerable scientific progress. Yet, it is crucial to retrospectively evaluate these efforts' societal impact. Research should be thoughtfully designed ensure that findings can effectively translated into actionable policies. addition, choice methods plays a pivotal role in shaping impact discoveries. Understanding factors responsible current unmet public health issues medical needs crafting innovative strategies policy interventions. A multistakeholder survey roundtable helped identify potential consideration EU agenda. Based findings, mental disorders, metabolic syndrome, cancer, antimicrobial resistance, environmental pollution, cardiovascular were considered challenges deserving prioritisation. early diagnosis, primary prevention, pollution onset personalised medicine approaches most selected needs. Survey enabled formulation some research-policies interventions (RPIs), which further discussed during online roundtable. The discussion underscored recent EU-level activities aligned with survey-derived RPIs facilitated an exchange perspectives topics ripe collaboration warranting attention EU's Actionable recommendations aimed at facilitating translation knowledge transformative, science-based policies are also provided.
Язык: Английский
Процитировано
2Nano Letters, Год журнала: 2024, Номер 24(47), С. 14993 - 15000
Опубликована: Окт. 30, 2024
Nicotinamide adenine dinucleotide (NAD) is a pivotal coenzyme, existing in its oxidized form (NAD
Язык: Английский
Процитировано
1Biophysica, Год журнала: 2024, Номер 4(4), С. 604 - 618
Опубликована: Ноя. 28, 2024
Tissue spheroids are self-organised 3D cellular aggregates that serve as a versatile platform in tissue engineering. While numerous high-throughput methods exist to characterise the function of spheroids, equivalent techniques for mechanical characterisation still lacking. In this review, we focus on fusion— simple, fast, and inexpensive method rheology spheroids. We begin by discussing implications development disease, followed detailed explanation how phenomenon arrested coalescence can be used explore Finally, present different theoretical models that, when combined with experimental data, allow us extract rheological information.
Язык: Английский
Процитировано
1Stem Cells Translational Medicine, Год журнала: 2023, Номер 13(3), С. 293 - 308
Опубликована: Дек. 30, 2023
Human adipose-derived stem cells (ASCs) have shown immense potential for regenerative medicine. Our previous work demonstrated that chitosan nano-deposited surfaces induce spheroid formation and differentiation of ASCs treating sciatic nerve injuries. However, the underlying cell fate mechanisms ASC-derived spheroids remain unknown. Here, we investigate epigenetic regulation signaling coordination these therapeutic spheroids. During formation, observed significant increases in histone 3 trimethylation at lysine 4 (H3K4me3), 9 (H3K9me3), 27 (H3K27me3), accompanied by increased deacetylase (HDAC) activities decreased acetyltransferase activities. Additionally, HDAC5 translocated from cytoplasm to nucleus, along with nuclear Utilizing single-cell RNA sequencing (scRNA-seq), analyzed chitosan-induced ASC discovered distinct cluster subpopulations, trajectories, traits, networks using 10x Genomics platform, R studio/language, Ingenuity Pathway Analysis (IPA) tool. Specific subpopulations were identified within corresponded a transient reprogramming state (Cluster 6) endpoint 3). H3K4me3 H3K9me3 as key regulators IPA initiate Cluster 6 cells, confirmed qPCR their respective methyltransferase inhibitors: SNDX-5613 (a KMT2A inhibitor H3K4me3) SUVi (an SUV39H1 H3K9me3). Moreover, involved regulating downstream neuronal markers during cells. These findings emphasize critical role regulation, particularly H3K4me3, H3K9me3, HDAC5, shaping directing lineage-specific differentiation.
Язык: Английский
Процитировано
3Current Stem Cell Research & Therapy, Год журнала: 2023, Номер 19(9), С. 1210 - 1250
Опубликована: Окт. 15, 2023
A number of studies have been conducted on the application 3D models for drug discovery, sensitivity assessment, and toxicity. Most these focused disease modelling attempted to control cellular differentiation, heterogeneity, key physiological features mimic organ reconstitution so that researchers could achieve an accurate response in evaluation. Recently, organoids used by various scientists due their highly organotypic structure, which facilitates translation from basic research clinic, especially cancer research. With this tool, can perform high-throughput analyses compounds determine exact effect patients based genetic variations, as well develop personalized combination therapies. Although there is a lack standardization organoid culture, patientderived (PDOs) become widely established testing. In review, we discussed recent advances tumoroids not only screening but also clinical trials demonstrate potential translational medicine.
Язык: Английский
Процитировано
2Medizinische Genetik, Год журнала: 2024, Номер 36(2), С. 121 - 131
Опубликована: Июнь 5, 2024
Язык: Английский
Процитировано
0Опубликована: Окт. 21, 2024
Язык: Английский
Процитировано
0bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Окт. 23, 2024
Summary The gene signatures of Alzheimer’s Disease (AD) brains reflect an output a complex interplay genetic, epigenetic, epi-transcriptomic, and post-transcriptional regulations. To identify the most significant factor that shapes AD brain signature, we developed machine learning model (DEcode-tree) to integrate cellular molecular factors explaining differential expression in AD. Our indicates YTHDF proteins, canonical readers N6-methyladenosine RNA modification (m6A), are influential predictors signature. We then show protein modules containing YTHDFs downregulated human brains, knocking out iPSC-derived neural cells recapitulates signature vitro . Furthermore, eCLIP-seq analysis revealed proteins influence through both m6A-dependent independent pathways. These results indicate central role shaping brains.
Язык: Английский
Процитировано
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