Applied Sciences,
Год журнала:
2024,
Номер
14(24), С. 12051 - 12051
Опубликована: Дек. 23, 2024
Aging
affects
the
structure
and
functions
of
all
organs
systems
in
organism.
In
elderly,
significant
changes
hormonal
levels
are
observed.
These
translate
to
a
predisposition
for
chronic
diseases,
including
cardiovascular,
neurodegenerative,
metabolic
disorders.
Therefore,
tremendous
scientific
effort
is
focused
on
investigating
molecular
mechanisms
drugs
with
potential
reduce
old
age
their
impact.
Sirtuin
1
(SIRT1),
member
sirtuin
family
deacetylases,
has
been
extensively
studied
as
regulator
multiple
pathways
related
antioxidant
properties,
optimal
immune
response,
metabolism.
SIRT1
plays
key
role
regulating
various
maintaining
homeostasis.
present
study,
we
review
interplay
between
regulations,
endocrine
hypothalamic–pituitary–thyroid,
–adrenal,
–gonadal,
–liver
axes,
other
glands,
non-endocrine
tissues
aging
The
application
natural
activators,
such
resveratrol,
curcumin,
paeonol,
Buyang
Huanwu
Decoction,
treatment
senescence
discussed.
activators
improve
mitochondrial
function,
oxidative
stress,
promote
longevity,
but
clinical
limited
by
low
bioavailability
poor
permeability
across
biological
barriers.
For
this
reason,
advanced
delivery
strategies
being
considered,
nose-to-brain
drug
nanotechnology-based
formulations.
Theranostics,
Год журнала:
2024,
Номер
14(8), С. 3358 - 3384
Опубликована: Янв. 1, 2024
With
the
increase
in
aging
population,
occurrence
of
neurological
disorders
is
rising.Recently,
stem
cell
therapy
has
garnered
attention
due
to
its
convenient
sourcing,
minimal
invasiveness,
and
capacity
for
directed
differentiation.However,
there
are
some
disadvantages,
such
as
poor
quality
control,
safety
assessments,
ethical
logistical
issues.Consequently,
scientists
have
started
shift
their
from
cells
extracellular
vesicles
similar
structures
properties.Beyond
these
parallels,
can
enhance
biocompatibility,
facilitate
easy
traversal
barriers,
minimize
side
effects.Furthermore,
cell-derived
be
engineered
load
drugs
modify
surfaces
treatment
outcomes.In
this
review,
we
summarize
functions
native
vesicles,
subsequently
review
strategies
engineering
applications
Alzheimer's
disease,
Parkinson's
stroke,
discuss
challenges
solutions
associated
with
clinical
translation
vesicles.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(11), С. 5863 - 5863
Опубликована: Май 28, 2024
Extracellular
vesicles
(EVs),
including
exosomes,
microvesicles,
and
other
lipid
derived
from
cells,
play
a
pivotal
role
in
intercellular
communication
by
transferring
information
between
cells.
EVs
secreted
progenitor
stem
cells
have
been
associated
with
the
therapeutic
effects
observed
cell-based
therapies,
they
also
contribute
to
tissue
regeneration
following
injury,
such
as
orthopaedic
surgery
cases.
This
review
explores
involvement
of
nerve
regeneration,
their
potential
drug
carriers,
significance
cell
research
cell-free
therapies.
It
underscores
importance
bioengineers
comprehending
manipulating
EV
activity
optimize
efficacy
engineering
regenerative
Cell Communication and Signaling,
Год журнала:
2024,
Номер
22(1)
Опубликована: Июль 22, 2024
Abstract
Stroke
is
a
leading
cause
of
mortality
and
long-term
disability
globally,
with
acute
ischemic
stroke
(AIS)
being
the
most
common
subtype.
Despite
significant
advances
in
reperfusion
therapies,
their
limited
time
window
associated
risks
underscore
necessity
for
novel
treatment
strategies.
Stem
cell-derived
extracellular
vesicles
(EVs)
have
emerged
as
promising
therapeutic
approach
due
to
ability
modulate
post-stroke
microenvironment
facilitate
neuroprotection
neurorestoration.
This
review
synthesizes
current
research
on
potential
stem
EVs
AIS,
focusing
origin,
biogenesis,
mechanisms
action,
strategies
enhancing
targeting
capacity
efficacy.
Additionally,
we
explore
innovative
combination
therapies
discuss
both
challenges
prospects
EV-based
treatments.
Our
findings
reveal
that
exhibit
diverse
effects
such
promoting
neuronal
survival,
diminishing
neuroinflammation,
protecting
blood-brain
barrier,
angiogenesis
neurogenesis.
Various
strategies,
including
modifications
cargo
modifications,
been
developed
improve
efficacy
EVs.
Combining
other
treatments,
therapy,
cell
transplantation,
nanomedicine,
gut
microbiome
modulation,
holds
great
promise
improving
outcomes.
However,
heterogeneity
need
standardized
protocols
EV
production
quality
control
remain
be
addressed.
represent
avenue
offering
address
limitations
Further
needed
optimize
translate
benefits
clinical
practice,
an
emphasis
ensuring
safety,
overcoming
regulatory
hurdles,
specificity
delivery
target
tissues.
Graphical
Pharmaceutics,
Год журнала:
2023,
Номер
15(9), С. 2173 - 2173
Опубликована: Авг. 22, 2023
Extracellular
vesicles
(EVs)
are
promising
therapeutic
modalities
for
treating
neurological
conditions.
EVs
facilitate
intercellular
communication
among
brain
cells
under
normal
and
abnormal
physiological
The
potential
capability
of
to
pass
through
the
blood-brain
barrier
(BBB)
makes
them
highly
as
nanocarrier
contenders
managing
stroke.
possess
several
advantages
compared
existing
drug-delivery
vehicles.
These
include
their
capacity
surpass
natural
barriers,
target
specific
cells,
stability
within
circulatory
system.
This
review
explores
trafficking
cellular
uptake
evaluates
recent
findings
in
field
research.
Additionally,
an
overview
is
provided
techniques
researchers
utilize
bioengineer
stroke
therapy,
new
results
on
EV-BBB
interactions,
limitations
prospects
clinically
using
therapies.
primary
objective
this
study
provide
a
comprehensive
analysis
challenges
related
engineered
drug
delivery,
specifically
focusing
application
treatment
Journal of physiological investigation.,
Год журнала:
2024,
Номер
67(2), С. 79 - 87
Опубликована: Март 1, 2024
Arteriosclerosis
(AS)
is
a
chronic
inflammatory
disease
and
Buyang
Huanwu
decoction
(BHD)
has
been
identified
as
an
anti-atherosclerosis
effect,
the
study
aimed
to
investigate
underlying
mechanism.
The
E4
allele
of
Apolipoprotein
E
(ApoE)
associated
with
both
metabolic
dysfunction
enhanced
pro-inflammatory
response,
ApoE-knockout
(ApoE-/-)
mice
were
fed
high-fat
diet
establish
arteriosclerosis
model
treated
BHD
or
atorvastatin
(as
positive
control).
atherosclerotic
plaque
in
each
mouse
was
evaluated
using
Oil
red
O
Staining.
Elisa
kits
used
evaluate
blood
lipid,
interleukin-6
(IL-6),
IL-1
beta
(IL-1β),
tumor
necrosis
factor
alpha
(TNF-α),
IL-4,
IL-10,
growth
(TGF-β)
contents,
while
Western
blot
applicated
measure
inducible
nitric
oxide
synthase
(iNOS),
arginase
I
(Arg-1)
expression.
Meanwhile,
pyruvate
kinase
M2
(PKM2),
hypoxia-inducible
factor-1
(HIF-1α)
its
target
genes
glucose
transporter
type
1
(GLUT1),
lactate
dehydrogenase
A
(LDHA),
3-phosphoinositide-dependent
(PDK1),
well
IL-6,
IL-1β,
TNF-α,
TGF-β
by
quantitative
reverse
transcription-polymerase
chain
reaction.
treatment
significantly
reduced
body
weight
area
lipid
levels
including
total
cholesterol
(TC),
triglyceride
(TG),
low-density
lipoprotein
(LDL-C),
high-density
(HDL-C).
demonstrated
significant
suppression
M1
polarization,
decreased
secretion
iNOS
factors
(IL-6,
TNF-α)
ApoE-/-
mice.
present
also
revealed
that
promotes
activation
characterized
expression
Arg-1
anti-inflammatory
(IL-4
IL-10).
In
addition,
PKM2/HIF-1α
signaling
improved
M1/M2
macrophages
polarization
induced
BHD.
downstream
(GLUT1,
LDHA,
PDK1)
increased
high
fat
feeding
mice,
those
which
recused
Atorvastatin.
These
results
suggested
produce
response
against
AS
progress
after
exposure.
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Окт. 23, 2024
Objective
We
aimed
to
evaluate
the
efficacy
of
stem
cell-derived
exosomes
for
treating
ischemic
stroke
and
screen
optimal
administration
strategy.
Methods
searched
PubMed,
Web
Science,
Embase,
Cochrane
Library,
Scopus
databases
relevant
studies
published
from
their
inception
31
December
2023.
Conventional
network
meta-analyses
routes
administration,
types,
immune
compatibility
were
performed
using
cerebral
infarct
volume
(%)
modified
neurological
severity
score
(mNSS)
as
outcome
indicators.
Results
A
total
38
randomized
controlled
animal
experiments
included.
meta-analysis
showed
that
compared
with
negative
control
group:
intravenous
significantly
reduced
mNSS;
intranasal
(%);
intracerebral
mNSS.
Adipose-derived
mesenchymal
(ADSC-Exos),
bone
marrow
(BMSC-Exos),
dental
pulp
(DPSC-Exos)
neural
(NSC-Exos)
Endothelial
progenitor
(EPC-Exos),
embryonic
(ESC-Exos),
induced
pluripotent
(iPSC-Exos)
(NPC-Exos)
Umbilical
cord
(UCMSC-Exos)
there
was
no
significant
difference
between
urogenital
(USC-Exos)
controls.
Engineered
had
better
than
unmodified
exosomes.
Both
allogeneic
xenogeneic
The
best
route
reducing
Among
10
types
administered
intravenously,
BMSC-Exos
type
Allogeneic
in
(%),
whereas
Conclusion
This
meta-analysis,
by
integrating
available
evidence,
revealed
is
are
exosome
type,
have
mNSS,
which
can
provide
options
preclinical
studies.
In
future,
more
high-quality
experiments,
especially
direct
comparative
needed
determine
strategy
stroke.
Systematic
Review
Registration:
https://www.crd.york.ac.uk/PROSPERO/display_record.php?ID=CRD42024497333
,
PROSPERO,
CRD42024497333
International Journal of Nanomedicine,
Год журнала:
2025,
Номер
Volume 20, С. 2253 - 2271
Опубликована: Фев. 1, 2025
Stroke
has
significantly
contributed
to
the
global
mortality
rate
over
years,
emphasizing
urgency
of
finding
effective
treatment
strategies.
Neural
stem
cell
(NSC)-derived
exosomes
have
potential
improve
neurological
recovery
after
stroke;
however,
their
therapeutic
efficacy
is
hindered
by
rapid
clearance
and
limited
duration
action.
This
study
presents
an
innovative
drug
delivery
method:
a
hydrogel
based
on
NSC
hydroxypropyl
methylcellulose
(HPMC),
which
intended
offer
continuous
release,
thereby
enhancing
prolonging
improvement.
We
developed
nanohydrogel
(Exo-HPMC)
integrating
Buyang
Huanwu
Decoction
(BHD)
-preconditioned
NSC-derived
with
HPMC.
thoroughly
investigated
controlled-release
capabilities
rheological
properties
Exo-HPMC.
Our
findings
show
that
Exo-HPMC
enables
sustained
exosome
extending
retention
in
mice.
When
administered
mice
middle
cerebral
artery
occlusion
(MCAO),
facilitated
notable
post-stroke
neurorepair.
Behavioral
assessments
immunofluorescence
staining
demonstrated
promoted
angiogenesis
nerve
regeneration
stroke-affected
areas,
reversing
programmed
death.
The
groundbreaking
approach
for
stroke
therapy.
Ensuring
controlled
prolonged
release
two
weeks,
enhances
ischemic
treatment.
