Journal of Zhejiang University (Medical Sciences),
Год журнала:
2025,
Номер
unknown
Опубликована: Май 1, 2025
To
investigate
the
effect
of
Shenge
powder
(SGP)
on
myocardial
fibrosis
in
rats
with
heart
failure
after
infarction
and
its
relation
lysyl
oxidase
like
protein
2
(LOXL2)/transforming
growth
factor-β1
(TGF-β1)/IL-11
signaling
pathway.
Seventy-two
SPF
male
SD
were
divided
into
blank
control
group,
model
SGP
small
dose
large
positive
dose+LOXL2
activator
12
each
group.
Except
for
post-myocardial
was
induced
by
coronary
constriction
all
groups.
Corresponding
treatments
given
immediately
successful
modeling,
once
a
day
4
weeks.
Left
ventricular
fractional
shortening
(LVFS)
left
ejection
fraction
(LVEF)
detected
Color
Doppler
ultrasound
imaging.
Levels
IL-1β
IL-6
serum
analyzed
ELISA
method,
Myocardial
collagen
volume
(CVF)
evaluated
Masson
staining.
Expressions
Ⅰ
α-smooth
muscle
actin
(α-SMA)
tissue
immunohistochemical
The
mRNA
expressions
matrix
metalloproteinase-9
(MMP-9)
inhibitor
metalloproteinase
1
(TIMP-1)
qRT-PCR.
Expression
LOXL2,
TGF-β1,
IL-11
proteins
Western
blotting.
Compared
LVFS
LVEF
group
decreased,
levels
elevated,
CVF
value,
α-SMA
tissue,
MMP-9
TIMP-1
mRNA,
increased
(all
P<0.05).
increased,
Collagen
decreased
(P<0.05);
while
LOXL2
reversed
improvement
high-dose
infarction.
may
inhibit
inhibiting
LOXL2/TGF-β1/IL-11
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
179, С. 117367 - 117367
Опубликована: Авг. 29, 2024
The
pyroptosis
of
cardiomyocytes
has
become
an
essential
topic
in
heart
failure
research.
abnormal
accumulation
these
biological
factors,
including
angiotensin
II,
advanced
glycation
end
products,
and
various
growth
factors
(such
as
connective
tissue
factor,
vascular
endothelial
transforming
factor
beta,
among
others),
activates
the
nuclear
factor-κB
(NF-κB)
signaling
pathway
cardiovascular
diseases,
ultimately
leading
to
cardiomyocytes.
Therefore,
exploring
underlying
molecular
mechanisms
is
for
developing
novel
drugs
therapeutic
strategies.
However,
our
current
understanding
precise
regulatory
mechanism
this
complex
cardiomyocyte
still
limited.
Given
this,
study
reviews
milestone
discoveries
field
research
since
1986,
analyzes
detail
similarities,
differences,
interactions
between
other
cell
death
modes
apoptosis,
necroptosis,
autophagy,
ferroptosis),
explores
deep
connection
failure.
At
same
time,
it
depicts
complete
activation,
transmission,
eventual
NF-κB
process
In
addition,
also
systematically
summarizes
approaches
that
can
inhibit
reduce
pyroptosis,
drugs,
natural
compounds,
small
molecule
inhibitors,
gene
editing,
cutting-edge
technologies,
aiming
provide
solid
scientific
support
new
perspectives
prevention
treatment
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(2), С. 848 - 848
Опубликована: Янв. 20, 2025
Tanshinones,
biologically
active
diterpene
compounds
derived
from
Salvia
miltiorrhiza,
interact
with
specific
proteins
and
DNA
sequences,
influencing
signaling
pathways
in
animals
humans.
This
study
highlights
tanshinone–protein
interactions
observed
at
concentrations
achievable
vivo,
ensuring
greater
physiological
relevance
compared
to
vitro
studies
that
often
employ
supraphysiological
ligand
levels.
Experimental
data
suggest
while
tanshinones
multiple
proteomic
targets,
only
a
few
enzymes
are
significantly
affected
relevant
concentrations.
apparent
paradox
may
be
resolved
by
tanshinones’
ability
bind
influence
involved
gene
expression
or
mRNA
stability,
such
as
RNA
polymerase
II
human
antigen
R
protein.
These
trigger
secondary,
widespread
changes
expression,
leading
complex
alterations.
Although
the
current
understanding
of
remains
incomplete,
this
provides
foundation
for
deciphering
molecular
mechanisms
underlying
therapeutic
effects
S.
miltiorrhiza
diterpenes.
Additionally,
numerous
tanshinone
derivatives
have
been
developed
enhance
pharmacokinetic
properties
biological
activity.
However,
their
safety
profiles
remain
poorly
characterized,
limiting
comprehensive
insights
into
medicinal
potential.
Further
investigation
is
essential
fully
elucidate
toxicological
both
native
modified
tanshinones.
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Янв. 24, 2025
Heart
failure
(HF)
has
emerged
as
a
significant
global
public
health
challenge
owing
to
its
high
rates
of
morbidity
and
mortality.
Activation
the
NOD-like
receptor
protein
3
(NLRP3)
inflammasome
is
regarded
pivotal
factor
in
onset
progression
HF.
Therefore,
inhibiting
activation
NLRP3
may
represent
promising
therapeutic
approach
for
preventing
treating
The
active
ingredients
serve
foundation
effects
traditional
Chinese
medicine
(TCM).
Recent
research
revealed
advantages
TCM
enhancing
cardiac
structure
function
study
aimed
explore
impact
on
HF,
review
current
advancements
utilizing
inhibit
This
provides
novel
perspective
future
development
precise
intervention
strategies
targeting
prevent
treat
Journal of Cellular and Molecular Medicine,
Год журнала:
2025,
Номер
29(3)
Опубликована: Фев. 1, 2025
ABSTRACT
Ischaemic
heart
disease
(IHD)
remains
a
leading
cause
of
global
morbidity
and
mortality.
One
significant
contributor
to
the
pathology
IHD
is
excessive
release
inflammatory
mediators
during
progression.
Pyroptosis
form
programmed
cell
death
(PCD)
triggered
by
activation
inflammasomes
caspase
1.
The
1
proteolytically
cleaves
gasdermin
D
(GSDMD)
activated
amino
acid
terminus
(GSDMD‐NT),
disruption
plasma
membrane.
This
cascade
events
considered
canonical
pathway
pyroptosis.
also
caused
oxidative
stress,
thereby
triggering
noncanonical
pyroptosis
via
caspases
4/5/11.
Previous
studies
have
provided
compelling
evidence
close
relationship
between
aetiology
(e.g.,
acute
myocardial
infarction,
ischaemia
reperfusion
injury
chronic
infarction),
as
well
association
with
unfavourable
clinical
outcomes.
Several
interventions
aimed
at
targeting
demonstrated
promising
therapeutic
benefits
against
IHD‐related
pathologies.
review
provides
mechanistic
insights
into
roles
in
from
vitro,
vivo
perspectives.
In‐depth
understanding
this
area
could
pave
way
for
future
development
novel
strategies
IHD.
Journal of Cardiothoracic Surgery,
Год журнала:
2025,
Номер
20(1)
Опубликована: Фев. 19, 2025
The
hypoxia/reoxygenation
(H/R)-induced
pyroptosis
of
cardiomyocytes
plays
a
crucial
role
in
the
pathogenesis
myocardial
infarction
(MI).
miR-155-5p
represents
promising
target
for
MI
therapy.
However,
its
involvement
H/R-induced
remains
unclear.
H/R
exposed
rat
cardiomyocyte
H9c2
was
utilized
as
vitro
model,
and
expression
levels
SIRT1
cells
were
modulated
through
cell
transfection
experiments.
Cell
proliferative
activity
assessed
using
counting
kit-8
assay.
Supernatant
lactate
dehydrogenase
(LDH)
determined
colorimetry.
living
dead
observed
via
Calcin-AM/PI
staining.
Levels
supernatant
interleukin
(IL)-1β
IL-18
measured
ELISA
silent
information
regulator
1
(SIRT1)
mRNA
detected
by
qRT-PCR.
protein
SIRT1,
NLRP3,
N-terminal
gasdermin
D
(GSDMD-N),
Cleaved
caspase-1
evaluated
Western
blot
analysis.
targeted
regulatory
relationship
between
verified
dual
luciferase
reporter
gene
proliferation
induced
attenuated,
accompanied
severe
injury,
increased
death,
release
substantial
amount
pro-inflammatory
cytokines
IL-1β
IL-18.
In
addition,
stimulation
resulted
upregulation
downregulation
cells.
Suppression
or
overexpression
exhibited
ameliorative
effects
on
cellular
injury
inhibited
NLRP3
inflammasome-mediated
pyroptosis.
dual-luciferase
assay
confirmed
direct
targeting
Furthermore,
partial
reversal
inhibitory
effect
inhibitor
upon
interference
with
expression.
Inhibition
alleviates
SIRT1-mediated
activation
inflammasome.
