Shenge powder inhibits myocardial fibrosis in rats with post-myocardial infarction heart failure through LOXL2/TGF-β1/IL-11 signaling pathway DOI Creative Commons
Hang Xie,

Boyong Qiu,

Haitao Li

et al.

Journal of Zhejiang University (Medical Sciences), Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

To investigate the effect of Shenge powder (SGP) on myocardial fibrosis in rats with heart failure after infarction and its relation lysyl oxidase like protein 2 (LOXL2)/transforming growth factor-β1 (TGF-β1)/IL-11 signaling pathway. Seventy-two SPF male SD were divided into blank control group, model SGP small dose large positive dose+LOXL2 activator 12 each group. Except for post-myocardial was induced by coronary constriction all groups. Corresponding treatments given immediately successful modeling, once a day 4 weeks. Left ventricular fractional shortening (LVFS) left ejection fraction (LVEF) detected Color Doppler ultrasound imaging. Levels IL-1β IL-6 serum analyzed ELISA method, Myocardial collagen volume (CVF) evaluated Masson staining. Expressions Ⅰ α-smooth muscle actin (α-SMA) tissue immunohistochemical The mRNA expressions matrix metalloproteinase-9 (MMP-9) inhibitor metalloproteinase 1 (TIMP-1) qRT-PCR. Expression LOXL2, TGF-β1, IL-11 proteins Western blotting. Compared LVFS LVEF group decreased, levels elevated, CVF value, α-SMA tissue, MMP-9 TIMP-1 mRNA, increased (all P<0.05). increased, Collagen decreased (P<0.05); while LOXL2 reversed improvement high-dose infarction. may inhibit inhibiting LOXL2/TGF-β1/IL-11

Language: Английский

Emodin activates autophagy to suppress oxidative stress and pyroptosis via mTOR-ULK1 signaling pathway and promotes multi-territory perforator flap survival DOI
Panfeng Wu, Xiao Yu,

Liming Qing

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 704, P. 149688 - 149688

Published: Feb. 14, 2024

Language: Английский

Citations

6
Mechanism of Qili Qiangxin Capsule for Heart Failure Based on miR133a-Endoplasmic Reticulum Stress DOI

Xiao-di Ji,

Yang Ding,

Xi-yuan Cui

et al.

Chinese Journal of Integrative Medicine, Journal Year: 2024, Volume and Issue: 30(5), P. 398 - 407

Published: Feb. 22, 2024

Language: Английский

Citations

4
Overview of pyroptosis mechanism and in-depth analysis of cardiomyocyte pyroptosis mediated by NF-κB pathway in heart failure DOI Open Access
Zeyu Zhang, Zhihua Yang, Shuai Wang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117367 - 117367

Published: Aug. 29, 2024

The pyroptosis of cardiomyocytes has become an essential topic in heart failure research. abnormal accumulation these biological factors, including angiotensin II, advanced glycation end products, and various growth factors (such as connective tissue factor, vascular endothelial transforming factor beta, among others), activates the nuclear factor-κB (NF-κB) signaling pathway cardiovascular diseases, ultimately leading to cardiomyocytes. Therefore, exploring underlying molecular mechanisms is for developing novel drugs therapeutic strategies. However, our current understanding precise regulatory mechanism this complex cardiomyocyte still limited. Given this, study reviews milestone discoveries field research since 1986, analyzes detail similarities, differences, interactions between other cell death modes apoptosis, necroptosis, autophagy, ferroptosis), explores deep connection failure. At same time, it depicts complete activation, transmission, eventual NF-κB process In addition, also systematically summarizes approaches that can inhibit reduce pyroptosis, drugs, natural compounds, small molecule inhibitors, gene editing, cutting-edge technologies, aiming provide solid scientific support new perspectives prevention treatment

Language: Английский

Citations

4
Mechanisms of mitochondrial damage-associated molecular patterns associated with inflammatory response in cardiovascular diseases DOI

Xiuju Guan,

Haitao Li,

Lijuan Zhang

et al.

Inflammation Research, Journal Year: 2025, Volume and Issue: 74(1)

Published: Jan. 13, 2025

Language: Английский

Citations

0
Proteins and DNA Sequences Interacting with Tanshinones and Tanshinone Derivatives DOI Open Access
Piotr Szymczyk, M. Majewska, Jadwiga Nowak

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 848 - 848

Published: Jan. 20, 2025

Tanshinones, biologically active diterpene compounds derived from Salvia miltiorrhiza, interact with specific proteins and DNA sequences, influencing signaling pathways in animals humans. This study highlights tanshinone–protein interactions observed at concentrations achievable vivo, ensuring greater physiological relevance compared to vitro studies that often employ supraphysiological ligand levels. Experimental data suggest while tanshinones multiple proteomic targets, only a few enzymes are significantly affected relevant concentrations. apparent paradox may be resolved by tanshinones’ ability bind influence involved gene expression or mRNA stability, such as RNA polymerase II human antigen R protein. These trigger secondary, widespread changes expression, leading complex alterations. Although the current understanding of remains incomplete, this provides foundation for deciphering molecular mechanisms underlying therapeutic effects S. miltiorrhiza diterpenes. Additionally, numerous tanshinone derivatives have been developed enhance pharmacokinetic properties biological activity. However, their safety profiles remain poorly characterized, limiting comprehensive insights into medicinal potential. Further investigation is essential fully elucidate toxicological both native modified tanshinones.

Language: Английский

Citations

0
Active ingredients of traditional Chinese medicine inhibit NOD-like receptor protein 3 inflammasome: a novel strategy for preventing and treating heart failure DOI Creative Commons

Ruifang Lin,

Yunfeng Yu,

Lixin Du

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 24, 2025

Heart failure (HF) has emerged as a significant global public health challenge owing to its high rates of morbidity and mortality. Activation the NOD-like receptor protein 3 (NLRP3) inflammasome is regarded pivotal factor in onset progression HF. Therefore, inhibiting activation NLRP3 may represent promising therapeutic approach for preventing treating The active ingredients serve foundation effects traditional Chinese medicine (TCM). Recent research revealed advantages TCM enhancing cardiac structure function study aimed explore impact on HF, review current advancements utilizing inhibit This provides novel perspective future development precise intervention strategies targeting prevent treat

Language: Английский

Citations

0
Therapeutic Potential of Gasdermin D‐Mediated Myocardial Pyroptosis in Ischaemic Heart Disease: Expanding the Paradigm From Bench to Clinical Insights DOI Creative Commons
Chanon Piamsiri, Chayodom Maneechote, Nipon Chattipakorn

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(3)

Published: Feb. 1, 2025

ABSTRACT Ischaemic heart disease (IHD) remains a leading cause of global morbidity and mortality. One significant contributor to the pathology IHD is excessive release inflammatory mediators during progression. Pyroptosis form programmed cell death (PCD) triggered by activation inflammasomes caspase 1. The 1 proteolytically cleaves gasdermin D (GSDMD) activated amino acid terminus (GSDMD‐NT), disruption plasma membrane. This cascade events considered canonical pathway pyroptosis. also caused oxidative stress, thereby triggering noncanonical pyroptosis via caspases 4/5/11. Previous studies have provided compelling evidence close relationship between aetiology (e.g., acute myocardial infarction, ischaemia reperfusion injury chronic infarction), as well association with unfavourable clinical outcomes. Several interventions aimed at targeting demonstrated promising therapeutic benefits against IHD‐related pathologies. review provides mechanistic insights into roles in from vitro, vivo perspectives. In‐depth understanding this area could pave way for future development novel strategies IHD.

Language: Английский

Citations

0
Signaling pathways behind the biological effects of tanshinone IIA for the prevention of cancer and cardiovascular diseases DOI

Homa Shahrokhi,

Javad Asili,

Zahra Tayarani-Najaran

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Language: Английский

Citations

0
Xin-Fu-Kang Oral Liquid Mitigates Chronic Heart Failure through NR4A1-Dependent Regulation of Endoplasmic Reticulum-Mitochondrial Crosstalk in Cardiomyocytes DOI
Xiaohan Zhang,

Xing Chang,

Ruoning Chai

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 140, P. 156467 - 156467

Published: Feb. 18, 2025

Language: Английский

Citations

0
Inhibition of mir-155-5p alleviates cardiomyocyte pyroptosis induced by hypoxia/reoxygenation via targeting SIRT1-mediated activation of the NLRP3 inflammasome DOI Creative Commons

Qiuyu Lu,

Qingrong Shen,

Jinmei Su

et al.

Journal of Cardiothoracic Surgery, Journal Year: 2025, Volume and Issue: 20(1)

Published: Feb. 19, 2025

The hypoxia/reoxygenation (H/R)-induced pyroptosis of cardiomyocytes plays a crucial role in the pathogenesis myocardial infarction (MI). miR-155-5p represents promising target for MI therapy. However, its involvement H/R-induced remains unclear. H/R exposed rat cardiomyocyte H9c2 was utilized as vitro model, and expression levels SIRT1 cells were modulated through cell transfection experiments. Cell proliferative activity assessed using counting kit-8 assay. Supernatant lactate dehydrogenase (LDH) determined colorimetry. living dead observed via Calcin-AM/PI staining. Levels supernatant interleukin (IL)-1β IL-18 measured ELISA silent information regulator 1 (SIRT1) mRNA detected by qRT-PCR. protein SIRT1, NLRP3, N-terminal gasdermin D (GSDMD-N), Cleaved caspase-1 evaluated Western blot analysis. targeted regulatory relationship between verified dual luciferase reporter gene proliferation induced attenuated, accompanied severe injury, increased death, release substantial amount pro-inflammatory cytokines IL-1β IL-18. In addition, stimulation resulted upregulation downregulation cells. Suppression or overexpression exhibited ameliorative effects on cellular injury inhibited NLRP3 inflammasome-mediated pyroptosis. dual-luciferase assay confirmed direct targeting Furthermore, partial reversal inhibitory effect inhibitor upon interference with expression. Inhibition alleviates SIRT1-mediated activation inflammasome.

Language: Английский

Citations

0