Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 158616 - 158616
Опубликована: Дек. 1, 2024
Life, Год журнала: 2024, Номер 14(6), С. 751 - 751
Опубликована: Июнь 12, 2024
Rheumatoid arthritis (RA) is a systemic autoimmune disorder caused by inflammation of cartilaginous diarthrodial joints that destroys and cartilage, resulting in synovitis pannus formation. Timely detection effective management RA are pivotal for mitigating inflammatory consequences, potentially influencing disease progression. Nuclear medicine using radiolabeled targeted vectors presents promising avenue diagnosis response to treatment assessment. Radiopharmaceutical such as technetium-99m (
Язык: Английский
Процитировано
3
Gigabyte, Год журнала: 2024, Номер 2024
Опубликована: Окт. 16, 2024
The extracellular matrix, composed of macromolecules like collagen fibres, provides structural support to cells and acts as a barrier that metastatic degrade spread beyond the primary tumour. While agent-based frameworks, such PhysiCell, can simulate spatial dynamics tumour evolution, they only implement circles (2D) or spheres (3D). To model matrix network we require new type agent represented by line segments cylinders Here, present PhysiMeSS, an addon introducing describe fibres their physical interactions with other fibres. PhysiMeSS implementation is available at https://github.com/PhysiMeSS/PhysiMeSS in official PhysiCell repository. We provide examples describing possibilities this framework. This tool may help tackle important biological questions, diseases linked dysregulation processes leading cancer metastasis.
Язык: Английский
Процитировано
3
Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(8), С. 9179 - 9214
Опубликована: Авг. 22, 2024
The recellularization of tissues after decellularization is a relatively new technology in the field tissue engineering (TE). Decellularization involves removing cells from or organ, leaving only extracellular matrix (ECM). This can then be recellularized with to create functional organs. first significant mention decellularized traced research conducted early 2000s. One landmark studies this was published 2008 by Ott, where researchers demonstrated rat heart cardiac cells, resulting organ capable contraction. Since then, other important have been published. These paved way for widespread application TE, demonstrating potential ECM serve as scaffold regenerating tissues. Thus, although concept initially explored previous decades, these 2000s marked major turning point development and practical article reviews historical advances limitations TE over last two decades.
Язык: Английский
Процитировано
2
Developmental Biology, Год журнала: 2024, Номер 516, С. 20 - 34
Опубликована: Июль 24, 2024
Ectodermal organs, such as hair follicles, originate from simple epithelial and mesenchymal sheets through a complex developmental process driven by interactions between these cell types. This involves dermal condensation, placode formation, bud morphogenesis, organogenesis, all of processes require intricate among various tissues. Recent research has emphasized the crucial role reciprocal dynamic cells extracellular matrix (ECM), referred to "dynamic duo", in development ectodermal organs. These provide spatially temporally changing biophysical biochemical cues within Using follicle an example, this review highlights two types cell-ECM adhesion units-focal adhesion-type hemidesmosome-type units-that facilitate communication cells. further explores how units, along with other interactions, evolve during regeneration, underscoring their importance guiding both regenerative processes.
Язык: Английский
Процитировано
2
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Янв. 12, 2024
ABSTRACT Direct observation of immune cell trafficking patterns and tumor-immune interactions is unlikely in human tumors with currently available technology, but computational simulations based on clinical data can provide insight to test hypotheses. It hypothesized that collagen formation evolve as a mechanism escape, the exact nature interaction between cells poorly understood. Spatial quantifying degree fiber alignment squamous carcinomas indicates late stage disease associated highly aligned fibers. Here, we introduce modeling framework (called Lenia) discriminate two hypotheses: migration moves 1) parallel or 2) perpendicular orientation. The recapitulates immune-ECM where protection, leading an emergent inverse relationship coverage. We also illustrate capabilities Lenia model evolution tumor progression predation. provides flexible for considering spectrum local (cell-scale) global (tumor-scale) dynamics by defining kernel cell-cell function governs growth under predation migration. Mathematical important mechanistic insights into interactions. Short-range kernels survival conditions strong Allee effects, while asymmetric lead poor response. Thus, length scale drives infiltration.
Язык: Английский
Процитировано
1
Regenerative Biomaterials, Год журнала: 2024, Номер 11
Опубликована: Янв. 1, 2024
Following peripheral nerve anastomosis, the anastomotic site is prone to adhesions with surrounding tissues, consequently impacting effectiveness of repair. This study explores development and efficacy a decellularized epineurium as an anti-adhesive biofilm in Firstly, entire was extracted from fresh porcine sciatic nerves, followed by decellularization process. The efficiency then thoroughly assessed. Subsequently, underwent proteomic analysis determine remaining bioactive components. To ensure biosafety, cytotoxicity assays, hemolysis tests, cell affinity assessments immune response following subcutaneous implantation. Finally, functionality determined using transection anastomosis model rats. result indicated that process effectively removed cellular components while preserving number molecules, this effective preventing adhesion promoting repairment functional recovery. In conclusion, represents novel promising anti-adhesion for enhancing surgical outcomes
Язык: Английский
Процитировано
1
iScience, Год журнала: 2024, Номер 27(8), С. 110477 - 110477
Опубликована: Июль 8, 2024
Rod-shaped fission yeast grows through cell wall expansion at poles and septum, synthesized by essential glucan synthases. Bgs1 synthesizes the linear β(1,3)glucan of primary septum cytokinesis. Linear is also present in poles, suggesting additional roles growth polarity. Our study reveals an collaboration between Tea1-Tea4, but not other polarity factors, controlling Simultaneous absence function Tea1-Tea4 causes complete loss polarity, spread synthases, spherical formation, indicating this defect specifically due to absence. Furthermore, induces actin patches delocalization sterols spread, which are ultimately responsible for without Tea1-Tea4. This suggests strong similarities functions structures during cytokinesis polarized growth. Collectively, our findings unveil that regulates growth, like equivalent extracellular matrix neuronal cells.
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 13, 2024
Abstract Many mammalian cells, including endothelial cells and fibroblasts, tend to align elongate with the orientation of extracellular matrix (ECM) fibers in a gel when cultured vitro. During cell elongation, clusters focal adhesions (FAs) form near poles elongating cells. FAs are mechanosensitive that grow under mechanical tension due cells’ pulling on ECM, shrink is released. Using mathematical modeling approach, we study hypothesis reciprocity between ECM drives shape changes. We show preferentially stabilized along fibers, where can generate more than perpendicular fibers. present hybrid cellular Potts model represents as an off-the-lattice network cross-linked deformable whereas represented lattice. Multiple modeled individually by independent rate FA assembly mechanoresponsive disassembly. The resulting computational predicts stiffness-dependent spreading local remodelling, ECM-alignment dependent elongation. effects combined suffice explain how morphology determined structure mechanics.
Язык: Английский
Процитировано
1
npj Systems Biology and Applications, Год журнала: 2024, Номер 10(1)
Опубликована: Сен. 30, 2024
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Окт. 27, 2024
Abstract Metastasis, the leading cause of death in cancer patients, arises when cells disseminate from a primary solid tumour to distant organs. Growth and invasion often involve collective cell migration, which is profoundly influenced by cell-cell interactions extracellular matrix (ECM). The ECM’s biochemical composition mechanical properties, such as stiffness, regulate behaviour migration dynamics. Mathematical modelling serves pivotal tool for studying predicting these complex dynamics, with hybrid discrete-continuous models offering powerful approach combining agent-based representations continuum descriptions surrounding microenvironment. In this study, we investigate impact ECM modulated via ribose-induced collagen cross-linking, on spheroid growth invasion. We employed model implemented PhysiCell simulate successfully replicating three-dimensional vitro experiments. incorporates detailed cell-ECM interactions, remodelling, proliferation. Our simulations align experimental observations two breast lines, non-invasive MCF7 invasive HCC1954, under varying stiffness conditions. results demonstrate that increased due cross-linking inhibits cells, whereas remain largely unaffected. Furthermore, our show higher degradation not only enables but also facilitates formation multicellular protrusions. Conversely, increasing maximum speed can reach enhances while promoting single-cell This understanding interplay between proliferation, paving way future studies incorporating additional characteristics microenvironmental
Язык: Английский
Процитировано
1