Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 158616 - 158616
Опубликована: Дек. 1, 2024
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown
Опубликована: Ноя. 22, 2022
Abstract Extracellular matrix (ECM) is a key component of the cellular microenvironment and critical in multiple disease developmental processes. Representing ECM cell-ECM interactions challenging multiscale problem as they span molecular-level details to tissue-level dynamics. While several computational frameworks exist for modeling, often focus on very detailed modeling individual fibers or represent only single aspect ECM. Using PhysiCell agent-based platform, we developed framework intermediate detail with ability capture bidirectional interactions. We small region ECM, an element, three variables describing its local microstructure: anisotropy, density, overall fiber orientation. To spatially model use array elements. Cells remodel microstructure turn, impacts motility. demonstrate utility this reusability core interaction through examples invasion, wound healing, basement membrane degradation, leader-follower collective migration. Despite relative simplicity framework, it able broad range interest community. Furthermore, representing are accessible simple programming interfaces. This allows them impact cell behaviors, such proliferation death, without requiring custom code each interaction, particularly PhysiCell’s grammar, enabling rapid diverse cell-matrix biology. make available free open source software package at https://github.com/PhysiCell-Models/collective-invasion .
Язык: Английский
Процитировано
4
Cureus, Год журнала: 2024, Номер unknown
Опубликована: Май 15, 2024
Background Human embryo vasculogenesis (blood vessel development starting from endothelial precursors) includes the ability of mesenchymal cells and pluripotent stem to differentiate into cells. Quantification progenitor is difficult assess during early steps human due several factors, especially paucity tissue which usually discarded after early-stage pregnancy abortive methods. CD133 (Promimin-1) a general marker cells, but combined with other markers such as CD34, it may identify embryonic development. CD34 immunohistochemistry was previously performed by our team capillaries comparatively microvessel density between different tissues. TIE2 an angiopoietin receptor strongly involved in newly formed blood maturation its expression some precursors for future pericytes. assesses presence single use does not evaluate state do nor do. Data about dynamics CD133/TIE2 stages are scarce. Hence, this study, we aimed dynamic CD133+ on five seven-week-old tissues special emphasis their vascular beds. Methodology followed quantification using Qu Path digital image analysis (DIA) automated method. Results showed divergent patterns initial phases development, specifically endothelium tiny capillaries. The lining perfused lumen gradually decreased embryos. It remained expressed greater intensity located at tip bud that emerged pre-existing much more specific than CD133, being restricted level endothelium; therefore, easier quantify analysis. aorta exception expression, were consistently co-expressed embryo. DIA assessment increased accuracy evaluation, first time they quantified software manually. Conclusions High heterogeneity observed well regions same gestational age. unique finding co-expression persistence inside aortic needs further studies elucidate role co-expression.
Язык: Английский
Процитировано
0
Journal of Orthopaedic Surgery and Research, Год журнала: 2024, Номер 19(1)
Опубликована: Июнь 27, 2024
Abstract Background Achilles tendon is vital in maintaining the stability and function of ankle joint. It quite difficult to achieve structural functional repair tissue engineering. Methods A tissue-engineered micro-tissue was prepared using rat tail extracellular matrix (TECM) combined with adipose stem cells (ADSCs) injuries. The TECM by repeated freezing thawing. vitro characteristics its effect on ADSCs proliferation were detected. This for vivo evaluated based general characteristics, gait analysis, ultrasound findings, histological biomechanical testing. Results results showed that scaffold had good biocompatibility ADSCs. At 2 weeks post-surgery, collagen types I III tenomodulin expression higher, vascular endothelial growth factor lower group than other groups. 4 8 analysis findings repaired smooth lustrous, arranged regularly evenly group. Gait confirmed better motor recovery noted In addition, mechanical properties Conclusion Tissue-engineered fabricated has can promote vivo. composite biomaterial broad application prospects
Язык: Английский
Процитировано
0
Soft Matter, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
The cellular Potts model, also known as the Glazier-Graner-Hogeweg is a lattice-based approach by which biological tissues at level of individual cells can be numerically studied. Traditionally, square or hexagonal underlying lattice structure assumed for two-dimensional systems, and this to introduce artifacts in dynamics model tissues. That is, on regular lattices, assume shapes that are dictated symmetries lattice. Here, we developed variant method applied broad class (ir)regular lattices. We show an irregular deriving from fluid-like configuration, two types removed. further report transition between disordered solid-like hexagonally ordered phase present monodisperse confluent function their surface tension. This shows hallmarks first-order different glass/jamming transitions commonly reported vertex active Voronoi models. emphasize analyzing distribution shape parameters found our state space. Our analysis provides useful reference future study epithelia using model.
Язык: Английский
Процитировано
0
Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 158616 - 158616
Опубликована: Дек. 1, 2024
Процитировано
0