Anemoside B4 attenuates necrotic enteritis of laying hens induced by Clostridium perfringens via inhibiting NF-κB and PI3K/Akt/mTOR signalling pathways

Heliyon, Год журнала: 2024, Номер 10(12), С. e33161 - e33161

Опубликована: Июнь 1, 2024

Poultry necrotic enteritis is an important enteric disease which might be controlled by antibiotics. However, with the excessive use of antibiotics, phenomenon drug resistance Clostridium perfringens becoming increasingly prominent. Anemoside B4 exhibits anti-inflammatory, antioxidant and immunomodulatory effects. This study was performed to estimate effect on chicken induced C. in vivo vitro. In experiment we investigated efficacy growth curve, biofilm formation, haemolytic activity, virulence-related gene expression NF-κB PI3K/AKT/mTOR activation Caco-2 cells perfringens. The results showed that 12.5–50 μg/mL had no antibacterial activity but could inhibit attenuate weaken PI3K/Akt/mTOR triggered cells. experiment, 60 17-day-old healthy White Leghorns were randomly divided into six groups. growing laying hens control group fed a basic diet, those five challenged groups diet (infection group), added 0.43 g/kg (0.43 Ane 0.86 (0.86 1.72 (1.72 group) 40 mg/kg lincomycin (lincomycin respectively. All infected 1 × 109 CFU from day 17–20. Blood intestinal samples obtained, data demonstrated improved blood biochemical parameters, attenuated jejunum tissue injury, increased spleen, thymus, bursa fabricius index, decreased lesion scores ileum. jejunum, downregulated IL-1β, IL-6, IL-10, TNF-α IFN-γ at mRNA levels. Moreover, significantly enhanced both protein levels tight junctions ZO-1, Claudin-1 MUC-2 jejunum. weakened p-P65, p-PI3K, p-Akt p-mTOR Diets supplemented alleviated perfringens-induced inhibiting signalling pathways improving barrier functions.

Язык: Английский

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THE ROLE OF TRADITIONAL CHINESE MEDICINE IN ADDRESSING THE CHALLENGES OF CLOSTRIDIAL GAS GANGRENE: A MULTIFACETED APPROACH

Pharmacological Research - Natural Products, Год журнала: 2025, Номер unknown, С. 100198 - 100198

Опубликована: Март 1, 2025

Язык: Английский

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Usnic acid and tannic acid as inhibitors of coccidia and Clostridium perfringens: alleviating necrotic enteritis and improving intestinal health in broiler chickens

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2025, Номер 16(1)

Опубликована: Май 12, 2025

Abstract Background Necrotic enteritis (NE) in broiler chickens leads to significant economic losses poultry production. This study examined the inhibitory effects of usnic acid and tannic on coccidia, sporozoite, Clostridium perfringens assessed their influence growth performance intestinal health NE-challenged broilers through vitro vivo experiments. Methods The experiment included 5 treatment groups: negative control (NC), 2 μmol/L diclazuril (DZ), 30 (UA), 90 (TA), 15 + 45 (UTA) groups. involved 320 divided into four PC (NE-challenged), SA (500 mg/kg salinomycin premix NE-challenged), UA (300 UTA 500 NE-challenged) Results In study, UA, TA, treatments significantly increased apoptosis coccidian oocysts sporozoites, lowered mitochondrial membrane potential ( P < 0.05), disrupted oocyst structure compared with those NC group. TA had C. , strongest inhibition observed results demonstrated that group presented improved d 13, 21, 28 whereas groups improvements 13 21 0.05). SA, reduced lesion scores by fecal counts from 19 Compared group, lower sIgA levels CD8 cell percentages a trend toward CD3 percentage = 0.069). serum diamine oxidase activity, crypt depth, platelet-derived factor mucosa while increasing villus height depth ratio number goblet cells also acetate butyrate concentrations cecum With respect gut microbiota, changes β diversity ileum were groups, indicating microbial community compositions differed among Romboutsia dominated Bacillales Lactobacillales Lachnospirales ileal microbiota. cecal Lactobacillus Butyricicoccus Blautia abundances elevated Conclusion Usnic induce coccidia sporozoites lowering potential. Both alone combination alleviate NE-induced adverse modulating immunity, altering composition, improving barrier function. alone, superior effects, providing promising basis for development effective feed additive combinations.

Язык: Английский

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Latest research progress on anti-microbial effects, mechanisms of action, and product developments of dietary flavonoids: A systematic literature review

Trends in Food Science & Technology, Год журнала: 2024, Номер 156, С. 104839 - 104839

Опубликована: Дек. 13, 2024

Язык: Английский

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A Supramolecular Antibiotic Targeting Drug-Resistant Pseudomonas aeruginosa through the Inhibition of Virulence Factors and Activation of Acquired Immunity

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(32), С. 41828 - 41842

Опубликована: Авг. 1, 2024

The bacterium

Язык: Английский

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Vaccination with formulations targeting Eimeria maxima and Clostridium perfringens conferred comprehensive protection using a dual-infection challenge model of necrotic enteritis

Poultry Science, Год журнала: 2024, Номер 104(2), С. 104687 - 104687

Опубликована: Дек. 17, 2024

Язык: Английский

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Isolation of a Virulent Clostridium perfringens Strain from Elaphurus davidianus and Characterization by Whole-Genome Sequence Analysis

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(7), С. 7169 - 7186

Опубликована: Июль 8, 2024

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