Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 2, 2023
Abstract
Biofilm-associated
candidiasis
poses
a
significant
challenge
in
clinical
settings
due
to
the
limited
effectiveness
of
existing
antifungal
treatments.
The
challenges
include
increased
pathogen
virulence,
multi-drug
resistance,
and
inadequate
penetration
antimicrobials
into
biofilm
structures.
One
potential
solution
this
problem
involves
development
novel
drugs
that
can
modulate
fungal
virulence
formation,
which
is
essential
for
pathogenesis.
Resistance
Candida
albicans
initiated
by
morphological
changes
from
yeast
hyphal
form.
This
transition
triggers
series
events
such
as
cell
wall
elongation,
adhesion,
invasion
host
tissues,
pathogenicity,
initiation
an
immune
response.
critical
interface
interactions
with
cells,
primarily
through
various
proteins,
particularly
mannoproteins.
Thus,
proteins
enzymes
are
considered
targets.
In
regard,
we
explored
α-glucosidase
our
target
plays
crucial
role
processing
Previous
studies
have
shown
inhibition
leads
defects
integrity,
reduced
diminished
secretion
hydrolytic
enzymes,
alterations
recognition,
pathogenicity.
Since
α-glucosidase,
converts
carbohydrates,
study
focuses
on
FDA-approved
carbohydrate
mimic
(Glycomimetics)
well-documented
applications
biological
contexts.
Through
virtual
screening
114
carbohydrate-based
drugs,
pseudo-sugar
Acarbose,
emerged
top
hit.
Acarbose
known
its
pharmacological
managing
type
2
diabetes
mellitus
targeting
α-glucosidase.
Our
preliminary
investigations
indicate
effectively
inhibits
C.
reduces
impairs
switching,
hinders
adhesion
all
at
very
low
concentrations
nanomolar
range.
Furthermore,
transcriptomic
analysis
reveals
mechanism
action
highlighting
Frontiers in Global Women s Health,
Год журнала:
2024,
Номер
5
Опубликована: Май 23, 2024
Introduction
Vaginal
Candida
colonization
(CC)
can
lead
to
vulvovaginal
candidiasis,
the
second
most
prevalent
vaginal
condition
worldwide,
and
has
been
associated
with
adverse
birth
outcomes.
However,
no
data
on
CC
in
Democratic
Republic
of
Congo
are
available.
We
investigated
prevalence,
species,
clinical
correlates,
risk
factors
pregnancy
outcomes
women
trimester
pregnancy.
Material
methods
In
Bukavu,
Congo,
pregnant
were
recruited
during
antenatal
care
between
16
20
weeks
gestation
from
January
2017
October
followed
until
delivery.
Sociodemographics,
sexual
behavioral,
hygienic
characteristics,
microbiological
collected.
detection
speciation
was
performed
microscopy
(Gram-stained
smears
wet-mount)
and/or
quantitative
PCR.
Multivariate
regression
models
used
estimate
different
associations
CC.
Results
The
prevalence
by
wet
mount,
Gram-stain
qPCR
27.9%,
28.1%
38.2%,
respectively.
C.
albicans
species
(91.0%).
Previous
genital
infections,
an
intermediate
microbiota,
bacterial
vaginosis,
use
pit
toilets
for
Clinically,
itching
only.
Women
had
twice
odds
preterm
birth,
if
concentration
high,
four
times
higher.
Conclusions
high
modifiable
factors.
Screening
treatment
should
be
as
a
possible
strategy
reduce
birth.
Journal of Clinical Medicine,
Год журнала:
2024,
Номер
13(17), С. 5163 - 5163
Опубликована: Авг. 30, 2024
Vulvovaginal
candidosis
(VVC)
represents
a
frequent
and
cumbersome
vaginal
infection.
Recurrent
and/or
persistent
infections
remain
common
among
significant
number
of
patients
despite
the
use
antifungals.
Probiotics
offer
promising
adjunctive
or
alternative
therapeutic
strategy
to
antifungals
in
management
VVC.
We
aimed
explore
thoroughly
examine
various
roles
potential
applications
probiotics
A
comprehensive
literature
search
was
conducted
identify
relevant
clinical
trials
systematic
reviews
that
effectiveness
treatment
prevention
VVC
recurrent
(rVVC).
Following
initial
screening
4563
articles,
total
25
studies
seven
were
finally
included
this
analysis.
The
reviewed
provide
generally
positive
yet
inconsistent
view
efficacy
managing
VVC,
including
clinical,
mycological
response,
perspectives.
Nonetheless,
fluconazole
remains
more
effective
than
treating
while
combination
two
seems
reduce
recurrence
improve
symptoms
significantly.
For
prevention,
seem
health
symptoms,
safety
tolerability
are
consistently
reported
across
studies,
affirming
represent
low-risk
intervention.
However,
clear
conclusions
difficult
establish
since
relative
different
endpoints
follow-up
times,
variable
populations
included,
used,
diverse
schedules
regimens
administered.
propose
future
should
study
benefit
well-defined
categories
such
as
(1)
with
acute
instead
antifungals,
(2)
adjuvant
probiotic
therapy
together
after
(3)
using
probiotics.
Biomedicines,
Год журнала:
2025,
Номер
13(2), С. 488 - 488
Опубликована: Фев. 17, 2025
Several
etiologic
factors
for
the
development
of
acute
leukemias
have
been
suggested;
however,
none
is
applicable
to
all
cases.
We
isolated
a
certain
mycovirus-containing
Aspergillus
flavus
(MCAF)
from
home
patient
with
lymphoblastic
leukemia.
Repeated
electron
microscopic
evaluations
proved
existence
mycovirus
in
this
organism.
According
chemical
analysis,
organism
does
not
produce
any
aflatoxin,
possibly
due
its
infestation
mycoviruses.
reported
that
using
ELISA
technique,
forty
pediatric
patients
leukemia
(ALL)
uniformly
had
antibodies
products
MCAF.
In
contrast,
three
separate
groups
controls,
consisting
normal
blood
donors,
individuals
solid
tumors,
and
sickle
cell
disease,
were
negative.
vitro
exposure
mononuclear
cells
ALL,
full
remission,
MCAF
induced
redevelopment
surface
phenotypes
genetic
markers
characteristic
ALL.
The
controls
incubation
ALL
lines
resulted
significant
cellular
apoptosis,
changes
cycle,
downregulation
transcription
factors,
including
PAX-5
Ikaros
(75
55
kDa).
Fungi
are
widespread
nature,
many
contain
Normally,
an
individual
inhales
1
10
fungal
spores
per
minute,
while
farmers
can
inhale
up
75,000
minute.
It
known
foresters,
who
more
exposed
fungi,
higher
rate
asthmatics,
most
whom
allergic
agents,
working
office
settings
lower
rate.
One
theories
suggests
predisposition
followed
by
infectious
agent.
With
above
findings,
we
propose
may
etiological
role
leukemogenesis
immune-depressed
genetically
susceptible
individuals.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Март 27, 2025
Background
Despite
the
pathogenesis
of
vulvovaginal
candidiasis
(VVC)
is
multifactorial,
this
study
aimed
to
assess
whether
phenotypic
characteristics,
such
as
biofilm
production
and
quality,
along
with
clinical
symptoms,
are
associated
recurrent
VVC
(RVVC).
Methods
Over
1
year
(Oct
2021–Oct
2022),
we
prospectively
included
271
patients
≥18
years
who
attended
our
institution,
had
Candida
spp.
isolated
in
vaginal
swabs,
provided
informed
consent.
Patients
were
followed
for
year.
isolates
tested
by
following
techniques:
crystal
violet
(CV)
biomass
quantification,
XTT
metabolic
activity
microscopy
area
quantification.
Clinical
microbiological
data
also
collected.
Results
Overall,
55
(20.3%)
experienced
at
least
one
recurrence,
19
(7.0%)
meeting
criteria
RVVC
(≥3
episodes/year),
65
episodes
total.
Demographic
characteristics
similar
both
groups.
Most
C.
albicans
(90.0%).
Median
(interquartile,
[IQR])
absorbance
values
CV
18/19
238/252
non-RVVC
follows:
CV,
1.850
(1.578–2.156)
vs.
1.426
(1.081–1.823),
p
=
0.005;
XTT,
0.184
(0.116–0.293)
0.228
(0.147–0.331),
0.253.
(IQR)
occupation
percentage
16/19
16/252
was,
respectively:
13.15
(8.54–16.9)
10.73
(5.88–17.73),
0.710.
Conclusion
was
high
production.
Additionally,
exhibited
a
tendency
toward
lower
activity,
which
may
contribute
treatment
failure.
Clinical Microbiology Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 22, 2025
SUMMARY
Vulvovaginal
candidiasis
(VVC)
is
a
prevalent
global
health
burden,
particularly
among
reproductive-aged
women.
Recurrent
VVC
affects
significant
proportion
of
this
population,
presenting
therapeutic
challenges.
The
predominant
pathogen,
Candida
albicans
,
opportunistically
transitions
from
commensal
organism
to
pathogen
when
microenvironmental
conditions
become
dysregulated.
Recently,
non-
species
have
gained
attention
for
their
reduced
antifungal
susceptibility
and
recurrence
tendencies.
Diagnosis
constrained
by
the
limitations
conventional
microbiological
techniques,
while
emerging
molecular
assays
offer
enhanced
detection
yet
lack
established
thresholds
differentiate
between
pathogenic
states.
Increasing
resistance
issues
are
encountered
traditional
azole-based
antifungals,
necessitating
innovative
approaches
that
integrate
microbiota
modulation
precision
medicine.
Therefore,
review
aims
systematically
explore
diversity,
drug
mechanisms,
biofilm
effects
species.
Vaginal
(VMB)
alterations
associated
with
were
also
examined,
focusing
on
interaction
Lactobacillus
spp.
fungi,
emphasizing
role
microbial
dysbiosis
in
disease
progression.
Finally,
potential
summarized,
particular
focus
use
probiotics
modulate
VMB
composition
restore
healthy
ecosystem
as
promising
treatment
strategy.
This
addresses
adopts
microbiota-centric
approach,
proposing
comprehensive
framework
personalized
management
reduce
improve
patient
outcomes.
Archiv der Pharmazie,
Год журнала:
2025,
Номер
358(5)
Опубликована: Май 1, 2025
Abstract
Female
reproductive
tract
infections
(RTIs)
are
a
major
health
challenge
worldwide
and
the
leading
cause
of
infertility
adverse
pregnancy
outcomes.
The
rising
incidence
RTIs
highlights
their
status
as
public
issue.
Microbial
dysbiosis,
particularly
bacterial,
fungal,
viral
infections,
constitutes
primary
etiological
factor
disrupting
female
health.
Antimicrobial
peptides
(AMPs)
evolutionarily
conserved
host
defense
molecules
that
exhibit
broad‐spectrum
antimicrobial
activity
against
pathogens,
well
anti‐inflammatory
immunomodulatory
properties.
This
review
systematically
summarizes
structural
diversity,
biological
sources,
mechanistic
pathways
human‐derived
AMPs
in
combating
RTIs,
with
particular
emphasis
on
therapeutic
potential
fertility
preservation.
Emerging
evidence
suggests
promising
alternatives
to
conventional
antibiotics
post‐antibiotic
era.
