Association of Antibody Responses to Helicobacter pylori Proteins with Colorectal Adenoma and Colorectal Cancer

Pathogens, Год журнала: 2024, Номер 13(10), С. 897 - 897

Опубликована: Окт. 14, 2024

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Impacts of Helicobacter pylori infection and eradication on gastrointestinal microbiota: An up-to-date critical review and future perspectives

Chinese Medical Journal, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 6, 2024

Abstract Helicobacter pylori ( H. ) infects approximately half of the population worldwide and causes chronic gastritis, peptic ulcers, gastric cancer. Test-and-treat strategies have been recommended for prevention -associated diseases. Advancements in high-throughput sequencing technologies broadened our understanding complex gastrointestinal (GI) microbiota its role maintaining host homeostasis. Recently, an increasing number studies indicated that colonization induces dramatic alterations microbiota, with a predominance reduction microbial diversity. Dysbiosis gut microbiome has also observed after infection, which may play development colorectal However, there is concern regarding impact antibiotics on during eradication. In this review, we summarize current literature concerning how infection reshapes GI underlying mechanisms, including changes environment, immune responses, persistent inflammation. Additionally, impacts eradication homeostasis use probiotics as adjuvant therapy are discussed. The shifts their crosstalk provide potential targets -related diseases extragastric manifestations.

Язык: Английский

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Comparative Assessment of the Anti-Helicobacter pylori Activity and Gastroprotective Effects of Three Herbal Formulas for Functional Dyspepsia In Vitro

Cells, Год журнала: 2024, Номер 13(11), С. 901 - 901

Опубликована: Май 24, 2024

Helicobacter pylori has been implicated in various gastrointestinal disorders, including functional dyspepsia. This study aimed to compare the anti-H. activity and gastroprotective effects of three typical herbal formulas used for disorders Korea: Shihosogan-tang (ST), Yijung-tang (YT), Pyeongwi-san (PS). Firstly, we assessed total phenolic flavonoid contents, as well antioxidative capacity. Additionally, evaluated antibacterial effect on H. using an ammonia assay, minimum inhibitory concentration (MIC) test, disk agar diffusion method. Furthermore, examined alterations gene expression tight junction proteins, pro-inflammatory cytokines, cellular vacuolation AGS cell model infected with pylori. While ST exhibited a higher content, superior free radical scavenging, inhibition compared YT PS, more evidently inhibited gastric morphological changes such vacuolation. All formulations significantly ameliorated inflammatory inflammation-related genes induced by infection. Overall, present vitro suggests that all possess potential ameliorating relatively excelling inhibiting infection inflammation, while potentially shows greater efficacy directly protecting mucosa.

Язык: Английский

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HtrA-Dependent E-Cadherin Shedding Impairs the Epithelial Barrier Function in Primary Gastric Epithelial Cells and Gastric Organoids

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(13), С. 7083 - 7083

Опубликована: Июнь 27, 2024

Impaired E-cadherin (Cdh1) functions are closely associated with cellular dedifferentiation, infiltrative tumor growth and metastasis, particularly in gastric cancer. The class-I carcinogen Helicobacter pylori (H. pylori) colonizes epithelial cells induces Cdh1 shedding, which is primarily mediated by the secreted bacterial protease high temperature requirement A (HtrA). In this study, we used human primary cell lines derived from gastroids mucosoids different healthy donors to investigate HtrA-mediated cleavage subsequent impact on pathogenesis a non-neoplastic context. We found severe impairment of HtrA-induced ectodomain 2D mucosoids. Since exhibit an intact apico-basal polarity, investigated transmigration across monolayer, was partially depolarized HtrA, as indicated microscopy, analyses transepithelial electrical resistance (TEER) colony forming unit (cfu) assays. Finally, CagA injection observed efficient translocation tyrosine phosphorylation and, lesser extent, similar effects summary, HtrA crucially important factor promoting multistep H. non-transformed organoid-based models.

Язык: Английский

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Helicobacter pylori vacA genes associated with gastric diseases in Trinidad and Tobago

IJID Regions, Год журнала: 2024, Номер 14, С. 100498 - 100498

Опубликована: Ноя. 22, 2024

Язык: Английский

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