Antibiofilm activity of carotenoid crocetin against Staphylococcal strains

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Май 13, 2024

Staphylococcus aureus and epidermidis stand as notorious threats to human beings owing the myriad of infections they cause. The bacteria readily form biofilms that help in withstanding effects antibiotics immune system. Intending combat biofilm formation reduce virulence pathogens, we investigated carotenoids, crocetin, crocin, on four Staphylococcal strains. Crocetin was found be most effective it diminished S. ATCC 6538 significantly at 50 µg/mL without exhibiting bactericidal effect (MIC >800 µg/mL) also inhibited by MSSA 25923 a concentration low 2 µg/mL, methicillin-resistant MW2 100 µg/mL. It displayed minimal no antibiofilm efficacy Gram-negative strains Escherichia coli O157:H7 Pseudomonas aeruginosa well fungal strain Candida albicans . could curb fibrils, which partly contributes Additionally, ADME analysis crocetin proclaims how relatively non-toxic chemical is. Also, synergistic characteristics combination with tobramycin. presence polyene chain carboxylic acid groups its ends is hypothesized contribute strong crocetin. These findings suggest using apocarotenoids, particularly might epidermidis.

Язык: Английский

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Antifungal, anti-biofilm, and anti-hyphal properties of N-substituted phthalimide derivatives against Candida species

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Июнь 5, 2024

Candida species comprise a ubiquitous pathogenic fungal genus responsible for causing candidiasis. They are one of the primary causatives several mucosal and systemic infections in humans can survive various environments. In this study, we investigated antifungal, anti-biofilm, anti-hyphal effects six N -substituted phthalimides against three species. Of derivatives, -butylphthalimide (NBP) was most potent, with minimum inhibitory concentration (MIC) 100 µg/ml which dose-dependently inhibited biofilm at sub-inhibitory concentrations (10–50 µg/ml) both fluconazole-resistant fluconazole-sensitive albicans parapsilosis . NBP also effectively formation other pathogens including uropathogenic Escherichia coli , Staphylococcus epidermidis aureus Vibrio parahaemolyticus along polymicrobial biofilms S. C. markedly hyphal cell aggregation altered its colony morphology dose-dependent manner. Gene expression analysis showed that significantly downregulated important hyphal- biofilm-associated genes, i.e., ECE1 HWP1 UME6 upon treatment. exhibited mild toxicity ranging from 2 to 20 nematode model. Therefore, study suggests has anti-biofilm antifungal potential strains.

Язык: Английский

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Activity of Organoboron Compounds against Biofilm-Forming Pathogens

Antibiotics, Год журнала: 2024, Номер 13(10), С. 929 - 929

Опубликована: Сен. 29, 2024

Bacteria have evolved and continue to change in response environmental stressors including antibiotics. Antibiotic resistance the ability form biofilms are inextricably linked, requiring continuous search for alternative compounds antibiotics that affect biofilm formation. One of latest drug classes is boron-containing compounds. Over last several decades, boron has emerged as a prominent element field medicinal chemistry, which led an increasing number being considered potential drugs. The focus this review on developments organic (BOCs) antimicrobial/anti-biofilm probes agents.

Язык: Английский

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Antimicrobial Efficacy of Trifluoro-Anilines Against Vibrio Species

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 623 - 623

Опубликована: Янв. 13, 2025

Vibrios are naturally present in marine ecosystems and commonly allied with live seafood. Vibrio species frequently cause foodborne infections, parahaemolyticus recently becoming a significant contributor to illness outbreaks. In response, aniline 68 of its derivatives were studied due their antibacterial effects targeting V. harveyi. Among these, 4-amino-3-chloro-5-nitrobenzotrifluoride (ACNBF) 2-iodo-4-trifluoromethylaniline (ITFMA) demonstrated both antibiofilm properties. The minimum inhibitory concentrations (MIC) for ACNBF ITFMA 100 µg/mL 50 µg/mL, respectively, against planktonic cells. active compounds effectively suppressed biofilm formation manner dependent on the dosage. Additionally, these trifluoro-anilines significantly reduced virulence factors such as motility, protease activity, hemolysis, indole production. Both caused noticeable destruction membrane bacterial cells and, at exhibited bactericidal activity within 30 min. Toxicity assays using Caenorhabditis elegans seed germination models showed that displayed mild toxicity. As result, inhibited growth formation. Furthermore, prevented surfaces shrimp squid models, highlighting potential use controlling seafood contamination.

Язык: Английский

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Preparation and evaluation of alizarin loaded liposomes for improved antibiofilm activities

Journal of Molecular Liquids, Год журнала: 2024, Номер unknown, С. 126545 - 126545

Опубликована: Ноя. 1, 2024

Язык: Английский

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