Control of Staphylococcus aureus infection by biosurfactant derived from Bacillus rugosus HH2: strain isolation, structural characterization, and mechanistic insights
Journal of Hazardous Materials,
Год журнала:
2024,
Номер
480, С. 136402 - 136402
Опубликована: Ноя. 5, 2024
Язык: Английский
Antibiofilm and Antivirulence Potentials of 3,2′-Dihydroxyflavone against Staphylococcus aureus
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(15), С. 8059 - 8059
Опубликована: Июль 24, 2024
Staphylococcus
aureus,
particularly
drug-resistant
strains,
poses
significant
challenges
in
healthcare
due
to
its
ability
form
biofilms,
which
confer
increased
resistance
antibiotics
and
immune
responses.
Building
on
previous
knowledge
that
several
flavonoids
exhibit
antibiofilm
activity,
this
study
sought
identify
a
novel
flavonoid
capable
of
effectively
inhibiting
biofilm
formation
virulence
factor
production
S.
aureus
strains
including
MRSA.
Among
the
19
flavonoid-like
compounds
tested,
3,2′-dihydroxyflavone
(3,2′-DHF)
was
identified
for
first
time
as
factors
with
an
MIC
75
µg/mL.
The
activity
further
confirmed
by
microscopic
methods.
Notably,
3,2′-DHF
at
5
µg/mL
effective
both
mono-
polymicrobial
biofilms
involving
Candida
albicans,
common
co-pathogen.
reduces
hemolytic
slime
production,
expression
key
such
hemolysin
gene
hla
nuclease
nuc1
aureus.
These
findings
highlight
potential
antivirulence
agent
against
bacterial
fungal
offering
promising
alternative
traditional
treatment
biofilm-associated
infections.
Язык: Английский
Antimicrobial Activity of Arthrospira platensis-Mediated Gold Nanoparticles against Streptococcus pneumoniae: A Metabolomic and Docking Study
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(18), С. 10090 - 10090
Опубликована: Сен. 19, 2024
The
emergence
of
antibiotic-resistant
Streptococcus
pneumoniae
necessitates
the
discovery
novel
therapeutic
agents.
This
study
investigated
antimicrobial
potential
green-synthesized
gold
nanoparticles
(AuNPs)
fabricated
using
Arthrospira
platensis
extract.
Characterization
Fourier
transform
infrared
spectroscopy
revealed
presence
functional
groups
such
as
ketones,
aldehydes,
and
carboxylic
acids
in
capping
agents,
suggesting
their
role
AuNP
stabilization.
Transmission
electron
microscopy
demonstrated
formation
rod-shaped
AuNPs
with
a
mean
diameter
134.8
nm,
determined
by
dynamic
light
scattering,
zeta
−27.2
mV,
indicating
good
colloidal
stability.
synthesized
exhibited
potent
antibacterial
activity
against
S.
pneumoniae,
minimum
inhibitory
concentration
(MIC)
12
μg/mL,
surpassing
efficacy
control
antibiotic,
tigecycline.
To
elucidate
underlying
mechanisms
action,
an
untargeted
metabolomic
analysis
A.
extract
was
performed,
identifying
26
bioactive
compounds
belonging
to
diverse
chemical
classes.
In
silico
studies
focused
on
molecular
docking
simulations
that
compound
22
strong
binding
affinity
topoisomerase
IV,
critical
enzyme
for
bacterial
DNA
replication.
Molecular
dynamics
further
validated
stability
this
protein–ligand
complex.
These
findings
collectively
highlight
promising
platensis-derived
constituent
compounds,
warranting
investigation
development
anti-pneumococcal
therapeutics.
Язык: Английский
Characterization and antibiofilm activity of carotenoids derived from marine Bacillus infantis against Staphylococcus aureus and Pseudomonas aeruginosa
International Journal of Environmental Health Research,
Год журнала:
2025,
Номер
unknown, С. 1 - 10
Опубликована: Фев. 10, 2025
Antibiotic
resistance
and
biofilm
pose
significant
challenges
in
healthcare,
impacting
economic
growth
human
well-being.
The
quest
must
be
conducted
for
plausible
natural
anti-biofilm
agents.
Liquid
chromatography
mass
spectroscopy
was
used
identification
of
carotenoids.
Thebiofilm
inhibition
eradication
activity
were
evaluated
against
P.
aeruginosa
S.
aureus
using
crystal
violet,
Congo
Red
agar,
Scanning
electron
microscopy
(SEM).
Along
with
apocarotenoids,
di‐O‐demethylspirilloxanthin,
dihydroxylycopene
glucoside,
2,2'-dihydroxy
astaxanthin,
all-trans-Rhodovibrin
detected.
Carotenoids
at
a
concentration
200
µg/ml
showed
89.42
±
3.42%,
44.72
6.18%
aureus,
aeruginosa,
respectively,
able
to
eradicate
25.68
1.87%
preformed
aureus.
SEM
Agar
confirmed
that
carotenoids
inhibited
bacterial
growth,
exopolysaccharide
production
prevented
formation.
Our
investigation
indicated
from
B.
infantis
could
an
effective
inhibitor
formed
by
both
organisms
also
had
good
Язык: Английский
Antibiofilm Activities of Multiple Halogenated Pyrimidines Against Staphylococcus aureus
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12830 - 12830
Опубликована: Ноя. 28, 2024
,
prevalent
in
hospital
and
community
settings,
forms
biofilms
that
are
highly
resistant
to
antibiotics
immune
responses,
complicating
treatment
contributing
chronic
infections.
These
challenges
underscore
the
need
for
novel
treatments
target
biofilm
formation
effectively
reduce
bacterial
virulence.
This
study
investigates
antibiofilm
antimicrobial
efficacy
of
halogenated
pyrimidine
derivatives
against
Язык: Английский