Control of Staphylococcus aureus infection by biosurfactant derived from Bacillus rugosus HH2: strain isolation, structural characterization, and mechanistic insights

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 136402 - 136402

Published: Nov. 5, 2024

Language: Английский

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Antibiofilm and Antivirulence Potentials of 3,2′-Dihydroxyflavone against Staphylococcus aureus

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8059 - 8059

Published: July 24, 2024

Staphylococcus aureus, particularly drug-resistant strains, poses significant challenges in healthcare due to its ability form biofilms, which confer increased resistance antibiotics and immune responses. Building on previous knowledge that several flavonoids exhibit antibiofilm activity, this study sought identify a novel flavonoid capable of effectively inhibiting biofilm formation virulence factor production S. aureus strains including MRSA. Among the 19 flavonoid-like compounds tested, 3,2′-dihydroxyflavone (3,2′-DHF) was identified for first time as factors with an MIC 75 µg/mL. The activity further confirmed by microscopic methods. Notably, 3,2′-DHF at 5 µg/mL effective both mono- polymicrobial biofilms involving Candida albicans, common co-pathogen. reduces hemolytic slime production, expression key such hemolysin gene hla nuclease nuc1 aureus. These findings highlight potential antivirulence agent against bacterial fungal offering promising alternative traditional treatment biofilm-associated infections.

Language: Английский

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Antimicrobial Activity of Arthrospira platensis-Mediated Gold Nanoparticles against Streptococcus pneumoniae: A Metabolomic and Docking Study

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10090 - 10090

Published: Sept. 19, 2024

The emergence of antibiotic-resistant Streptococcus pneumoniae necessitates the discovery novel therapeutic agents. This study investigated antimicrobial potential green-synthesized gold nanoparticles (AuNPs) fabricated using Arthrospira platensis extract. Characterization Fourier transform infrared spectroscopy revealed presence functional groups such as ketones, aldehydes, and carboxylic acids in capping agents, suggesting their role AuNP stabilization. Transmission electron microscopy demonstrated formation rod-shaped AuNPs with a mean diameter 134.8 nm, determined by dynamic light scattering, zeta −27.2 mV, indicating good colloidal stability. synthesized exhibited potent antibacterial activity against S. pneumoniae, minimum inhibitory concentration (MIC) 12 μg/mL, surpassing efficacy control antibiotic, tigecycline. To elucidate underlying mechanisms action, an untargeted metabolomic analysis A. extract was performed, identifying 26 bioactive compounds belonging to diverse chemical classes. In silico studies focused on molecular docking simulations that compound 22 strong binding affinity topoisomerase IV, critical enzyme for bacterial DNA replication. Molecular dynamics further validated stability this protein–ligand complex. These findings collectively highlight promising platensis-derived constituent compounds, warranting investigation development anti-pneumococcal therapeutics.

Language: Английский

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Characterization and antibiofilm activity of carotenoids derived from marine Bacillus infantis against Staphylococcus aureus and Pseudomonas aeruginosa

International Journal of Environmental Health Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 10

Published: Feb. 10, 2025

Antibiotic resistance and biofilm pose significant challenges in healthcare, impacting economic growth human well-being. The quest must be conducted for plausible natural anti-biofilm agents. Liquid chromatography mass spectroscopy was used identification of carotenoids. Thebiofilm inhibition eradication activity were evaluated against P. aeruginosa S. aureus using crystal violet, Congo Red agar, Scanning electron microscopy (SEM). Along with apocarotenoids, di‐O‐demethylspirilloxanthin, dihydroxylycopene glucoside, 2,2'-dihydroxy astaxanthin, all-trans-Rhodovibrin detected. Carotenoids at a concentration 200 µg/ml showed 89.42 ± 3.42%, 44.72 6.18% aureus, aeruginosa, respectively, able to eradicate 25.68 1.87% preformed aureus. SEM Agar confirmed that carotenoids inhibited bacterial growth, exopolysaccharide production prevented formation. Our investigation indicated from B. infantis could an effective inhibitor formed by both organisms also had good

Language: Английский

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Antibiofilm Activities of Multiple Halogenated Pyrimidines Against Staphylococcus aureus

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12830 - 12830

Published: Nov. 28, 2024

, prevalent in hospital and community settings, forms biofilms that are highly resistant to antibiotics immune responses, complicating treatment contributing chronic infections. These challenges underscore the need for novel treatments target biofilm formation effectively reduce bacterial virulence. This study investigates antibiofilm antimicrobial efficacy of halogenated pyrimidine derivatives against

Language: Английский

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