In Vitro Chronic Hyperinsulinemia Induces Remodelling of Vascular Smooth Muscle Cells from Young Men and Women in a Sex Hormone Independent Manner DOI Creative Commons

Ashley Jazzar,

Danielle Jacques,

Amira Abou-Aichi

и другие.

Pathophysiology, Год журнала: 2025, Номер 32(1), С. 12 - 12

Опубликована: Март 13, 2025

Elevated circulating insulin levels between 80 and 100 µU/mL characterize hyperinsulinemia, which often leads to metabolic disorders such as obesity, resistance, type 2 diabetes (T2D). can directly affect vascular function contribute the pathophysiology of cardiovascular system, including secondary arterial hypertension (SAH) atherosclerosis. It is well known that hyperinsulinemia induced remodeling heart. However, there no information on whether intrinsic differences exist human smooth muscle cells (VSMCs) if in vitro mimicking induces VSMCs morphological intracellular homeostasis a sex- sex hormones-dependent manner. Our cultured VSMCs, coupled with quantitative 3D confocal imaging results, show from young men women. Chronic (80 µU/mL, 48 h treatment) increases cell nuclear volumes associated increased calcium (Ca2+) ROS decreased glutathione. In absence pretreatment testosterone oestradiol women had effect. Both hormones partially but not completely prevented hyperinsulinemia-induced The increase VSMC volume may thickness tunica media, leading decrease lumen blood vessel, promotes development SAH atherosclerosis sex-dependent

Язык: Английский

The emerging role of oxidative stress in inflammatory bowel disease DOI Creative Commons

P. De Muro,

Li Zhang, Shuxuan Li

и другие.

Frontiers in Endocrinology, Год журнала: 2024, Номер 15

Опубликована: Июль 15, 2024

Inflammatory bowel disease (IBD) is a chronic immune-mediated condition that affects the digestive system and includes Crohn’s (CD) ulcerative colitis (UC). Although exact etiology of IBD remains uncertain, dysfunctional immunoregulation gut believed to be main culprit. Amongst immunoregulatory factors, reactive oxygen species (ROS) nitrogen (RNS), components oxidative stress event, are produced at abnormally high levels in IBD. Their destructive effects may contribute disease’s initiation propagation, as they damage lining activate inflammatory signaling pathways, further exacerbating inflammation. Oxidative markers, such malondialdehyde (MDA), 8-hydroxy-2’-deoxyguanosine (8-OHdG), serum-free thiols (R-SH), can measured blood stool patients with These markers elevated IBD, their correlate severity disease. Thus, used not only diagnosis but also monitoring response treatment. It targeted treatment through use antioxidants, including vitamin C, E, glutathione, N-acetylcysteine. In this review, we summarize role pathophysiology its diagnostic targets, potential application antioxidant therapies manage treat

Язык: Английский

Процитировано

31

Oxidative stress in critically ill neonatal foals DOI Creative Commons
David M. Wong, Dipak Kumar Sahoo,

Cosette M. Faivre

и другие.

Journal of Veterinary Internal Medicine, Год журнала: 2025, Номер 39(1)

Опубликована: Янв. 1, 2025

Abstract Background Oxidative injury occurs in septic people, but the role of oxidative stress and antioxidants has rarely been evaluated foals. Objectives/Hypothesis To measure reactive oxygen species (ROS), biomarkers injury, neonatal We hypothesized that ill foals would have higher blood concentrations ROS lower compared to healthy Animals Seventy‐two hospitalized 21 Methods Prospective cohort study. Reactive (hydrogen peroxide [H 2 O ]), (malondialdehyde [MDA], protein carbonyl), (superoxide dismutase [SOD], catalase [CAT], glutathione, glutathione reductase [GR] peroxidase [GPx]) were measured from at admission. Measured variables between using a 1‐way ANOVA by Tukey's multiple comparisons test. Results Ill (n = 51) had significantly mean H (healthy 2.6 ± 1.4 nmol/mL, 6.8 4.6 L nmol/mL; 95% CI), MDA 31.2 14.4 114.3 94.0 carbonyl 0.07 0.01 nmol/mg protein, 0.12 0.02 CI). Significant CAT 0.4 0.3 mU/mg 238.5 101.9 μg/mL, 110.7 37.8 CI; P < .0001), GR 1.6 1.8 0.5 GPx 0.003 0.007 0.002 CI) also noted. Conclusions Clinical Importance antioxidant occur bacteremic These should be considered during treatment

Язык: Английский

Процитировано

2

Curcumin‐loaded nanoparticles effectively prevent T4‐induced oxidative stress in rat heart DOI
Pallavi Mishra, Dipak Kumar Sahoo, Chandana Mohanty

и другие.

Cell Biochemistry and Function, Год журнала: 2024, Номер 42(4)

Опубликована: Июнь 1, 2024

Abstract In this study, we report the cardioprotective effect of glycerol monooleate (GMO) based nanocurcumin in both vitro and vivo conditions under a hyperthyroid state. The heart is one primary target organs sensitive to action thyroid hormone, slight variations hormone serum concentrations result measurable changes cardiac performance. Hyperthyroidism‐induced hypermetabolism associated with oxidative stress an important mechanism responsible for progression failure. Curcumin has been known play protective role against stress‐related diseases like Alzheimer's, asthma, aging due its antioxidant properties. Nevertheless, potent biological activity hindered poor bioavailability. To overcome drawback, GMO‐based biodegradable nanoparticle (NP) formulation loaded curcumin developed, curcumin‐loaded NPs was compared native drug. Oxidative parameters reactive oxygen species (ROS) release, change mitochondrial membrane permeability, lipid peroxidation (LPx), lactate dehydrogenase (LDH) protein expression endogenous enzymes superoxide dismutase, catalase (CAT) glutathione peroxidase were evaluated. results from showed that better DPPH NO radical scavenging than range 2.5–20 µM. It also observed nanoparticulate comparatively more effective protecting ROS‐induced damage by reducing LPx LDH leakage at low 5–10 Further, performed facilitating activities respect time concentrations, resulting reduced cellular ROS levels. scenario, anticipate can serve as counterpart diseases.

Язык: Английский

Процитировано

4

Oxidative stress pattern of patients with abnormal thyroid function in Southern Nigeria DOI

O O Festus,

Ebenezer O Dic-Ijiewere,

Felix Ailemen

и другие.

Journal of Research in Applied and Basic Medical Sciences, Год журнала: 2025, Номер 11(1), С. 85 - 97

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Six-Day Fasting Causes Temporary Increases in Both Antioxidant Capacity and Oxidative Stress in Healthy Young Men: A Randomized Controlled Trial DOI Creative Commons
Marius Brazaitis, Katerina Židonienė, Nerijus Eimantas

и другие.

Antioxidants, Год журнала: 2025, Номер 14(3), С. 269 - 269

Опубликована: Фев. 26, 2025

The impact of prolonged fasting on human oxidative stress (OS) levels and antioxidant defence mechanisms remains poorly understood. aim this current study was to investigate the redox response a 6-day fast in cohort healthy men. Twenty-six participants were randomly allocated complete or control trial. Sympathetic activity, substrate oxidation, status, blood glucose, ketones, testosterone concentrations assessed. Throughout period, ketone concentration fat oxidation increased, carbohydrate glucose decreased. Heart rate increased days 2 4 returned pre-fasting level day 6. Malondialdehyde (MDA) after 6, increase accompanied by an total capacity (TAC), but TAC/MDA ratio remained constant. Notably, all fasting-evoked changes baseline values resumption regular diet. Thus, activated both OS, balance maintained. Consistent with response, sympathetic nervous system activity These variables state usual eating habits.

Язык: Английский

Процитировано

0

In Vitro Chronic Hyperinsulinemia Induces Remodelling of Vascular Smooth Muscle Cells from Young Men and Women in a Sex Hormone Independent Manner DOI Creative Commons

Ashley Jazzar,

Danielle Jacques,

Amira Abou-Aichi

и другие.

Pathophysiology, Год журнала: 2025, Номер 32(1), С. 12 - 12

Опубликована: Март 13, 2025

Elevated circulating insulin levels between 80 and 100 µU/mL characterize hyperinsulinemia, which often leads to metabolic disorders such as obesity, resistance, type 2 diabetes (T2D). can directly affect vascular function contribute the pathophysiology of cardiovascular system, including secondary arterial hypertension (SAH) atherosclerosis. It is well known that hyperinsulinemia induced remodeling heart. However, there no information on whether intrinsic differences exist human smooth muscle cells (VSMCs) if in vitro mimicking induces VSMCs morphological intracellular homeostasis a sex- sex hormones-dependent manner. Our cultured VSMCs, coupled with quantitative 3D confocal imaging results, show from young men women. Chronic (80 µU/mL, 48 h treatment) increases cell nuclear volumes associated increased calcium (Ca2+) ROS decreased glutathione. In absence pretreatment testosterone oestradiol women had effect. Both hormones partially but not completely prevented hyperinsulinemia-induced The increase VSMC volume may thickness tunica media, leading decrease lumen blood vessel, promotes development SAH atherosclerosis sex-dependent

Язык: Английский

Процитировано

0