Benefits of Immobilized Bacteria in Bioremediation of Sites Contaminated with Toxic Organic Compounds

Microorganisms, Год журнала: 2025, Номер 13(1), С. 155 - 155

Опубликована: Янв. 14, 2025

Although bioremediation is considered the most environmentally friendly and sustainable technique for remediating contaminated soil water, it effective when combined with physicochemical methods, which allow preliminary removal of large quantities pollutants. This allows microorganisms to efficiently eliminate remaining contaminants. In addition requiring necessary genes degradation pathways specific substrates, as well tolerance adverse environmental conditions, may perform below expectations. One typical reason this high toxicity xenobiotics present in concentrations, stemming from vulnerability bacteria introduced a site. especially true planktonic bacteria, whereas within biofilms or microcolonies have significant advantages over their counterparts. A physical matrix essential formation, maintenance, survival bacterial biofilms. By providing such immobilization, formation can be facilitated accelerated. Therefore, immobilization offers comprehensive solution cleanup by harnessing specialized metabolic activities while ensuring retention efficacy at target sites. many cases, also need methods that are otherwise required initially reduce contaminant concentrations. Then, will possible use remediation higher concentrations xenobiotics, significantly reducing costs maintaining rapid rate processes. review explores benefits highlighting materials processes developing an optimal matrix. It focuses on following four key areas: (i) types organic pollutants impacting human health, (ii) strains used processes, (iii) (iv) cells various carriers targeted pollutant degradation.

Язык: Английский

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The Potential for Restoring the Activity of Oxidoreductases and Hydrolases in Soil Contaminated with Petroleum Products Using Perlite and Dolomite

Applied Sciences, Год журнала: 2024, Номер 14(9), С. 3591 - 3591

Опубликована: Апрель 24, 2024

The research focused on assessing the response of oxidoreductases (dehydrogenases and catalase) hydrolases (urease, acid phosphatase, alkaline arylsulfatase, β-glucosidase) to diesel oil (DO) gasoline (G) contamination soils subjected phytoremediation with Zea mays. activity enzymes constitutes one fundamental mechanisms for removal contaminants from soil, which have potential contaminate not only soil but also groundwater water reservoirs. Additionally, correlations between enzyme basic physicochemical properties were determined. interaction perlite dolomite cultivated plant was tested. study carried out in a pot experiment, where contaminated DO or G artificially treated at doses 0, 8 cm3, 16 cm3 kg−1. Perlite applied remediation 0 10 g kg−1 soil. mays found respond tested pollutant reduction biomass. affected growth this more than G. reduced yield aerial parts by 86% 74%. negative effects these pollutants development mitigated both dolomite. exerted greater pressure hydrolases, as well enhanced most whereas inhibited them. implementation intensified all enzymes, except AcP (acid phosphatase) Glu (ß-glucosidase), G, improved its properties. induced less significant

Язык: Английский

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Bioremediation strategies for xenobiotic degradation in petroleum-impacted industrial ecosystems: Practical challenges and future directions

Journal of Water Process Engineering, Год журнала: 2025, Номер 70, С. 106877 - 106877

Опубликована: Янв. 5, 2025

Язык: Английский

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Sustainable Remediation of Polluted Soils from the Oil Industry Using Sludge from Municipal Wastewater Treatment Plants

Processes, Год журнала: 2025, Номер 13(1), С. 245 - 245

Опубликована: Янв. 16, 2025

Soil pollution with hydrocarbons is a consequence of activities associated the petroleum industry and related sectors. The effects are devastating, making remediation contaminated sites imperative. Consequently, soil decontamination represents significant costly challenge for industry. article proposes dual-recovery bioremediation solution that both efficient cost-effective, exploring potential use dehydrated sewage sludge from municipal wastewater treatment plants to treat petroleum-contaminated soils. Over three-month experiment, changes in density indigenous bacteria samples, treated or untreated sludge, were monitored along reduction hydrocarbon concentrations. In parallel, evolution other contaminants, such as heavy metals, was during experiment. Geotechnical tests also conducted evaluate feasibility returning its original location after Our results demonstrate proposed method effectively addresses soils (hazardous waste) reuse plants.

Язык: Английский

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Emerging strategies for enhancing microbial degradation of petroleum hydrocarbons: Prospects and challenges

Bioresource Technology Reports, Год журнала: 2024, Номер 26, С. 101866 - 101866

Опубликована: Май 23, 2024

Язык: Английский

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Biodegradation of polycyclic aromatic hydrocarbons: The role of ligninolytic enzymes and advances of biosensors for in-situ monitoring

Emerging contaminants, Год журнала: 2024, Номер 11(1), С. 100424 - 100424

Опубликована: Окт. 13, 2024

Язык: Английский

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From Killer to Solution: Evaluating Bioremediation Strategies on Microbial Diversity in Crude Oil-Contaminated Soil over Three to Six Months in Port Harcourt, Nigeria

Advances in Environmental and Engineering Research, Год журнала: 2024, Номер 05(04), С. 1 - 26

Опубликована: Ноя. 5, 2024

The study aimed to evaluate the efficacy of various bioremediation approaches on microbial diversity in crude oil-contaminated soil over three six months Port Harcourt, Nigeria. objective was assess impact different strategies populations, particularly focusing hydrocarbon-utilizing bacteria and fungi. Microbial populations were quantified using serial dilution count techniques. vapor phase transfer mechanism employed estimate Bacterial fungal colonies incubated for five days, followed by biochemical tests isolate identification. Fungal pure cultures observed under a microscope. significant increase free oil pollution when bioremediators such as mushrooms earthworms introduced. Mushrooms exhibited 50% (HUB), while showed 55% HUB six-month period. longer lifespan nutrient absorption capabilities facilitated faster growth. Furthermore, growth population fungi oil-polluted after employing bioremediation, with highest treated at months, months. Conversely, lowest recorded polluted 10% remediated results suggest that effectively soil. However, demonstrated higher compared earthworms, especially terms promoting (HUB) (HUF). Based findings, it is recommended prioritize agents similar environmental restoration efforts due their superior increasing HUF. This underscores potential effective restoring soil, offering insights sustainable practices oil-affected regions like

Язык: Английский

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Sustainable Bioremediation of Oily Sludge: Optimizing Co-Composting with Chicken Manure and Sawdust

Опубликована: Янв. 1, 2025

Improper disposal of oily sludge (OS), which is the result accumulation hydrocarbons in storage tanks, a serious threat to environment due presence toxic substances. Also, high volume chicken manure (CM) production requires comprehensive and efficient management. This study investigates feasibility composting with sawdust (SD) as an environmentally sustainable bioremediation method. Four experimental compost treatments including different ratios sludge, were conducted for 13 weeks. Physicochemical, biological microbial parameters investigated during co-composting process. Gas chromatography-mass spectrometry (GC-MS) used detect hydrocarbon compounds. The results showed significant decrease total organic carbon (TOC), Kjeldahl nitrogen (TKN), C/N ratio, petroleum (TPH) coliform bacteria, while pH electrical conductivity (EC) gradually increased. It was also seen that after eighth week treatment, it better time maturity most treatments. However, third fourth gave responses compared other types treatment. Among treatments, Treatment 4 optimum 99.24% TPH degradation, ensuring degree stability grade B criteria. Therefore, may be promising inexpensive oil wastes environmental pollution remediation.

Язык: Английский

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Optimizing Laccase Production From Halotolerant Enterobacter sp. GR18 for Hydrocarbon Bioremediation

Remediation Journal, Год журнала: 2025, Номер 35(2)

Опубликована: Янв. 1, 2025

ABSTRACT Hydrocarbon contamination, primarily from oil spills and their derivatives, poses significant environmental challenges has profound impacts on public health ecosystems. Innovative bioremediation strategies, especially those utilizing laccase enzymes, have emerged as robust sustainable solutions to degrade these persistent pollutants. This study focused optimizing production Enterobacter sp. GR18 , isolated the Grawan mineral spring near Sardasht, West Azerbaijan Province, Iran. The bacterium was identified through comprehensive biochemical tests 16S rRNA sequencing. Advanced methodologies were used optimize conditions. purified enzyme characterized using SDS‐PAGE. effects of various carbon nitrogen sources, metal ions, inducers enzymatic activity thoroughly investigated. Structural changes in crude samples treated with analyzed nuclear magnetic resonance (NMR) spectroscopy. showed exceptional among 33 strains. Glucose yeast extract most effective respectively. Copper iron ions significantly enhanced activity. NMR spectroscopy indicated substantial breakdown complex hydrocarbons posttreatment, demonstrating enzyme's effectiveness hydrocarbon degradation. reveals potential for high efficient degradation under extreme These findings can catalyze development technologies address contamination.

Язык: Английский

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Bioremediation Potential of Indigenous Bacterial Isolates for Treating Petroleum Hydrocarbons-Induced Environmental Pollution

ACS Omega, Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

This study isolates oil-degrading bacteria from contaminated soil in automobile workshops, aiming to address environmental pollution petroleum spills. Nineteen bacterial were screened, with three S4 (Lysinibacillus macroides), M8 (Serratia marcescens), and M9 (S. showing significant oil degradation potency. In shake flask assays, degraded 57.8%, 60.2%, 58.1% of at a 5% concentration. Molecular identification through 16S rRNA gene sequencing confirmed these strains. Plate assays also supported the potential, growth zones 38.4 ± 1.3 mm (S4), 39.7 1.9 (M8), 41.2 1.7 (M9). Gas chromatography-mass spectrometry analysis treated indicated presence hydrocarbons, phenolic compounds, esters, suggesting microbial interactions, potentially involving breakdown or transformation complex hydrocarbons into simpler compounds by enzymes. These results highlight potential strains, particularly S4, for bioremediation petroleum-contaminated sites.

Язык: Английский

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