CRISPR/Cas9-mediated genome editing ofFrankliniella occidentalis,the western flower thrips, via embryonic microinjection DOI Creative Commons
Jinlong Han,

William Klobasa,

Lucas de Oliveira

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 1, 2023

Abstract The western flower thrips, Frankliniella occidentalis , poses a significant challenge in global agriculture as notorious pest and vector of economically orthotospoviruses. However, the limited availability genetic tools for F. hampers advancement functional genomics development innovative control strategies. In this study, we present robust methodology generating heritable mutations using CRISPR/Cas9 genome editing system. Two eye-color genes, white ( Fo-w ) cinnabar Fo-cn ), frequently used to assess Cas9 function insects were identified targeted knockout through embryonic microinjection complexed with or specific guide RNAs. Homozygous lines established by crossing mutant females males. line revealed an age-dependent modification phenotype. Specifically, while young larvae exhibit ivory-colored eyes, color transitions bright red they age. Unexpectedly, loss also altered body color, mutants having lighter colored than wild type, suggesting dual role thrips. contrast, individuals from consistently displayed eyes throughout all life stages. Molecular analyses validated precise both target genes. This study offers powerful tool investigate thrips gene functions paves way technologies population suppression and/or replacement means mitigating virus transmission vector.

Язык: Английский

Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management DOI Creative Commons

J. Komal,

H. R. Desai,

Ipsita Samal

и другие.

Plants, Год журнала: 2023, Номер 12(23), С. 3961 - 3961

Опубликована: Ноя. 24, 2023

Phytophagous insects pose a significant threat to global crop yield and food security. The need for increased agricultural output while reducing dependence on harmful synthetic insecticides necessitates the implementation of innovative methods. utilization CRISPR-Cas (Clustered regularly interspaced short palindromic repeats) technology develop insect pest-resistant plants is believed be highly effective approach in production expenses enhancing profitability farms. Insect genome research provides vital insights into gene functions, allowing better knowledge biology, adaptability, development targeted pest management disease prevention measures. editing technique has capability modify DNA insects, either trigger drive or overcome their resistance specific insecticides. advancements CRISPR its various applications have shown potential developing insect-resistant varieties other strategies through sustainable approach. This could consequences ensuring involves using create modified plants. article critically analyzed discussed challenges associated with exploring utilizing pressure

Язык: Английский

Процитировано

15
Opsin mutants alter host plant selection by color vision in the nocturnal invasive pest Tuta absoluta DOI Creative Commons

Yanhong Tang,

Siyan Bi,

Xiaodi Wang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 265, С. 130636 - 130636

Опубликована: Март 11, 2024

In insects, vision is crucial in finding host plants, but its role nocturnal insects largely unknown. Vision involves responses to specific spectra of photon wavelengths and opsins plays an important this process. Long-wavelength sensitive opsin (LW opsin) blue-sensitive (BL are main visual proteins play behavior regulation.We used CRISPR/Cas9 technology mutate the long-wavelength-sensitive blue wavelength-sensitive genes explored invasive pest Tuta absoluta. Light wave experiments revealed that LW2 (−/−) BL(−/−) mutants showed abnormal wavelength tropism. Both BL mutations affected preference T. absoluta for green environment. Mutations necessary inhibit attraction. The elimination leaf moths both induced a white plants. Behavioral changes resulting from LW2(−/−) were not by sense smell, further supporting regulatory insect behavior. To best our knowledge, first study reveal vision, host-seeking at night, which key factors. These findings will drive development "vision-ecology" theory.

Язык: Английский

Процитировано

4
CRISPR/Cas9‐mediated genome editing of Frankliniella occidentalis, the western flower thrips, via embryonic microinjection DOI Creative Commons
Jinlong Han,

William Klobasa,

Lucas de Oliveira

и другие.

Insect Molecular Biology, Год журнала: 2024, Номер 33(6), С. 589 - 600

Опубликована: Апрель 27, 2024

The western flower thrips, Frankliniella occidentalis, poses a significant challenge in global agriculture as notorious pest and vector of economically orthotospoviruses. However, the limited availability genetic tools for F. occidentalis hampers advancement functional genomics development innovative control strategies. In this study, we present robust methodology generating heritable mutations using CRISPR/Cas9 genome editing system. Two eye-colour genes, white (Fo-w) cinnabar (Fo-cn), frequently used to assess Cas9 function insects were identified targeted knockout through embryonic microinjection complexed with Fo-w or Fo-cn specific guide RNAs. Homozygous lines established by crossing mutant females males. line revealed an age-dependent modification phenotype. Specifically, while young larvae exhibit orange-coloured eyes, colour transitions bright red they age. Unexpectedly, loss also altered body colour, mutants having lighter coloured than wild type, suggesting dual role thrips. contrast, individuals from consistently displayed eyes throughout all life stages. Molecular analyses validated precise both target genes. This study offers powerful tool investigate thrips gene paves way technologies population suppression and/or replacement means mitigating virus transmission vector.

Язык: Английский

Процитировано

4
Pioneering genome editing in parthenogenetic stick insects: CRISPR/Cas9-mediated gene knockout in Medauroidea extradentata DOI Creative Commons
Giulia Di Cristina,

Elina Dirksen,

Benjamin Altenhein

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 20, 2025

Язык: Английский

Процитировано

0
An overview of sustainable management strategies for Tuta absoluta DOI Creative Commons
Kahsay Tadesse Mawcha, Grace Kinyanjui, Daniel Hagos Berhe

и другие.

International Journal of Pest Management, Год журнала: 2025, Номер unknown, С. 1 - 24

Опубликована: Фев. 4, 2025

Язык: Английский

Процитировано

0
Modelling potential distribution of Tuta absoluta in China under climate change using CLIMEX and MaxEnt DOI Open Access
Jinyu Zhao, Li Ma, Chengfei Song

и другие.

Journal of Applied Entomology, Год журнала: 2023, Номер 147(10), С. 895 - 907

Опубликована: Сен. 14, 2023

Abstract The Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) originated in South America and is a major pest of the economically critical solanaceous crops. It devastating to tomatoes, attacking mainly leaves, stems, flowers fruits tomato plants, can cause up 100% damage. T. invaded China 2017 expanded rapidly, severely impacting industry. To illustrate detailed potential distribution China, we used CLIMEX MaxEnt models predict this using historical future climate data. predicts wider area for than MaxEnt, which suggests that most suitable its distribution, except Tarim Basin southern Xinjiang, western Inner Mongolia northwestern Gansu. Both accurately predicted known provinces Yunnan Guangxi, predictions by both suggest total range increase slightly with changes temperature. These will help understand influence change on thus provide theoretical foundation developing early monitoring, quarantine control strategies.

Язык: Английский

Процитировано

7
Genome Assembly and Population Sequencing Reveal Three Populations and Signatures of Insecticide Resistance ofTuta absolutain Latin America DOI Creative Commons
Kyle M. Lewald, Christine A. Tabuloc, Kristine Godfrey

и другие.

Genome Biology and Evolution, Год журнала: 2023, Номер 15(4)

Опубликована: Апрель 1, 2023

Abstract Tuta absoluta is one of the largest threats to tomato agriculture worldwide. Native South America, it has rapidly spread throughout Europe, Africa, and Asia over past two decades. To understand how T. been so successful improve containment strategies, high-quality genomic resources an understanding population history are critical. Here, we describe a highly contiguous annotated genome assembly, as well genome-wide analysis samples collected across Latin America. The new assembly L50 17 with only 132 contigs. Based on hundreds thousands single nucleotide polymorphisms, detect three major clusters in America some evidence admixture along Andes Mountain range. coalescent simulations, find these diverged from each other tens generations ago prior domestication tomatoes. We further identify several loci patterns consistent positive selection that related insecticide resistance, immunity, metabolism. This data will future research toward genetic control strategies inform policies.

Язык: Английский

Процитировано

6
Gene editing in agricultural, health, and veterinary pest arthropods: recent advances DOI

M. H. Manjunatha,

Michael N. Pham, Monika Gulia-Nuss

и другие.

Current Opinion in Insect Science, Год журнала: 2024, Номер 65, С. 101235 - 101235

Опубликована: Июль 15, 2024

Язык: Английский

Процитировано

0
Genome editing: a novel approach to manage insect vectors of plant viruses DOI
Sumit Jangra, Jesse Potts, Amalendu Ghosh

и другие.

Insect Biochemistry and Molecular Biology, Год журнала: 2024, Номер 174, С. 104189 - 104189

Опубликована: Сен. 27, 2024

Язык: Английский

Процитировано

0
Pioneering Genome Editing in Parthenogenetic Stick Insects: CRISPR/Cas9-Mediated gene knockout in Medauroidea extradentata DOI Creative Commons
Giulia Di Cristina,

Elina Dirksen,

Benjamin Altenhein

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 29, 2024

Abstract The parthenogenetic life cycle of the stick insect Medauroidea extradentata offers unique advantages for generation transgenic lines, as an isogenic and stable line can in principle be achieved already first generation. However, genetic tools manipulation their genes had not been developed until now. Here, we successfully implement CRISPR/Cas9 a technique to modify genome extradentata. As proof-of-concept targeted two involved ommochrome pathway eye pigmentation (cinnabar white, second exon, respectively), generate knockout (KO) mutants. Microinjections were performed within 24h after oviposition, focus on monocellular (and haploid) stage development. KOs generated resulted distinct cuticle colour phenotypes cinnabar white. Homozygous mutants showed pale eyes cuticle, while homozygous white KO completely unpigmented phenotype developing embryos. In conclusion, show that applied M. by creating phenotypically different viable animals. This toolbox now employed create genetically modified lines using non-model organism.

Язык: Английский

Процитировано

0