Frontiers in Genetics,
Год журнала:
2023,
Номер
14
Опубликована: Фев. 23, 2023
Introduction:
Abdominal
aortic
aneurysms
(AAA)
are
among
the
most
lethal
non-cancerous
diseases.
A
comprehensive
analysis
of
AAA-related
disease
model
has
yet
to
be
conducted.
Methods:
Weighted
correlation
network
(WGCNA)
was
performed
for
genes.
Machine
learning
random
forest
and
LASSO
regression
were
develop
score.
Immune
characteristics
epigenetic
score
explored.
Results:
Our
study
developed
a
reliable
predicting
immunity
m1A/m5C/m6A/m7G
regulation.
Discussion:
The
pathogenic
roles
four
genes,
UBE2K,
TMEM230,
VAMP7,
PUM2,
in
AAA,
need
further
validation
by
vitro
vivo
experiments.
Molecular Biomedicine,
Год журнала:
2023,
Номер
4(1)
Опубликована: Авг. 24, 2023
Abstract
RNA
modifications
are
dynamic
and
reversible
chemical
on
substrate
that
regulated
by
specific
modifying
enzymes.
They
play
important
roles
in
the
regulation
of
many
biological
processes
various
diseases,
such
as
development
cancer
other
diseases.
With
help
advanced
sequencing
technologies,
role
has
caught
increasing
attention
human
diseases
scientific
research.
In
this
review,
we
briefly
summarized
basic
mechanisms
several
common
modifications,
including
m6A,
m5C,
m1A,
m7G,
Ψ,
A-to-I
editing
ac4C.
Importantly,
discussed
their
potential
functions
cancer,
neurological
disorders,
cardiovascular
metabolic
genetic
developmental
well
immune
disorders.
Through
“writing-erasing-reading”
mechanisms,
regulate
stability,
translation,
localization
pivotal
disease-related
mRNAs
to
manipulate
disease
development.
Moreover,
also
highlighted
review
all
currently
available
RNA-modifier-targeting
small
molecular
inhibitors
or
activators,
most
which
designed
against
m6A-related
enzymes,
METTL3,
FTO
ALKBH5.
This
provides
clues
for
clinical
therapy
future
study
directions
modification
field.
More
in-depth
studies
further
activators
needed
a
thorough
understanding
epitranscriptomics
diagnosis,
treatment,
prognosis
Molecular Therapy — Nucleic Acids,
Год журнала:
2023,
Номер
31, С. 295 - 308
Опубликована: Янв. 11, 2023
In
recent
years,
N7-methylguanosine
(m7G)
methylation,
originally
considered
as
messenger
RNA
(mRNA)
5′
caps
modifications,
has
been
identified
at
defined
internal
positions
within
multiple
types
of
RNAs,
including
transfer
ribosomal
miRNA,
and
mRNAs.
Scientists
have
put
substantial
efforts
to
discover
m7G
methyltransferases
methylated
sites
in
RNAs
unveil
the
essential
roles
modifications
regulation
gene
expression
determine
association
dysregulation
various
diseases,
neurological
disorders.
Here,
we
review
findings
regarding
distribution,
abundance,
biogenesis,
modifiers,
functions
modifications.
We
also
provide
an
up-to-date
summary
detection
profile
mapping
techniques,
databases
for
validated
predicted
sites,
web
servers
methylation
prediction.
Furthermore,
discuss
pathological
METTL1/WDR-driven
Last,
outline
a
roadmap
future
directions
trends
modification
research,
particularly
central
nervous
system.
Frontiers in Oncology,
Год журнала:
2023,
Номер
13
Опубликована: Март 9, 2023
Most
of
our
transcribed
RNAs
are
represented
by
non-coding
sequences.
Long
(lncRNAs)
transcripts
with
no
or
very
limited
protein
coding
ability
and
a
length
>200nt.
They
can
be
epigenetically
modified.
N6-methyladenosine
(m6A),
N1-methyladenosine
(m1A),
5-methylcytosine
(m5C),
7-methylguanosine
(m7G)
2’-O-methylation
(Nm)
some
the
lncRNAs
epigenetic
modifications.
The
modifications
RNA
controlled
three
classes
enzymes,
each
playing
role
in
specific
phase
modification.
These
enzymes
defined
as
“writers”,
“readers”
“erasers”.
m6A
m5C
most
studied
RNA.
alter
structure
properties,
thus
modulating
functions
interactions
lncRNAs.
aberrant
expression
several
is
linked
to
development
variety
cancers
signatures
m6A-
m5C-related
increasingly
recognized
potential
biomarkers
prognosis,
predictors
disease
stage
overall
survival.
In
present
manuscript,
up
date
literature
reviewed
focus
on
their
significance
cancer.
Abstract
Cancer
is
one
of
the
leading
causes
death
worldwide,
and
more
effective
ways
attacking
cancer
are
being
sought.
immunotherapy
a
new
therapeutic
method
after
surgery,
radiotherapy,
chemotherapy,
targeted
therapy.
aims
to
kill
tumor
cells
by
stimulating
or
rebuilding
body's
immune
system,
with
specific
efficiency
high
safety.
However,
only
few
patients
respond
due
complex
variable
characters
escape,
behavior
regulatory
mechanisms
need
be
deeply
explored
from
dimensions.
Epigenetic
modifications,
metabolic
modulation,
cell‐to‐cell
communication
key
factors
in
cell
adaptation
response
microenvironment.
They
collectively
determine
state
function
through
modulating
gene
expression,
changing
energy
nutrient
demands.
In
addition,
engage
networks
other
components,
which
mediated
exosomes,
cytokines,
chemokines,
pivotal
shaping
progression
response.
Understanding
interactions
combined
effects
such
multidimensions
modulation
important
for
revealing
failure
developing
targets
strategies.
Experimental & Molecular Medicine,
Год журнала:
2024,
Номер
56(6), С. 1235 - 1249
Опубликована: Июнь 14, 2024
It
is
apparent
that
various
functional
units
within
the
cellular
machinery
are
derived
from
RNAs.
The
evolution
of
sequencing
techniques
has
resulted
in
significant
insights
into
approaches
for
transcriptome
studies.
Organisms
utilize
RNA
to
govern
systems,
and
a
heterogeneous
class
RNAs
involved
regulatory
functions.
In
particular,
increasingly
recognized
participate
intricately
functioning
across
almost
all
levels
biological
systems.
These
systems
include
those
mediating
chromatin
arrangement,
transcription,
suborganelle
stabilization,
posttranscriptional
modifications.
Any
exhibiting
activity
can
be
termed
typically
represented
by
noncoding
RNAs,
which
constitute
substantial
portion
genome.
function
based
on
principle
structural
changes
through
cis
and/or
trans
regulation
facilitate
mutual
RNA‒RNA,
RNA‒DNA,
RNA‒protein
interactions.
not
been
clearly
elucidated
whether
identified
deep
actually
anticipated
mechanisms.
This
review
addresses
dominant
properties
at
layers
covers
activities,
dynamics,
modifications,
associated
molecules,
further
challenges
related
therapeutics
learning.
Epigenetics & Chromatin,
Год журнала:
2024,
Номер
17(1)
Опубликована: Окт. 16, 2024
Cancer
has
arisen
from
both
genetic
mutations
and
epigenetic
changes,
making
epigenetics
a
crucial
area
of
research
for
innovative
cancer
prevention
treatment
strategies.
This
dual
perspective
propelled
into
the
forefront
research.
review
highlights
important
roles
DNA
methylation,
histone
modifications
non-coding
RNAs
(ncRNAs),
particularly
microRNAs
(miRNAs)
long
RNAs,
which
are
key
regulators
cancer-related
gene
expression.
It
explores
potential
epigenetic-based
therapies
to
revolutionize
patient
outcomes
by
selectively
modulating
specific
markers
involved
in
tumorigenesis.
The
examines
promising
biomarkers
early
detection
prognosis.
also
recent
progress
oligonucleotide-based
therapies,
including
antisense
oligonucleotides
(ASOs)
antimiRs,
precisely
modulate
processes.
Furthermore,
concept
editing
is
discussed,
providing
insight
future
role
precision
medicine
patients.
integration
nanomedicine
therapy
been
explored
offers
approaches
improve
therapeutic
efficacy.
comprehensive
advances
seeks
advance
field
oncology,
ultimately
culminating
improved
fight
against
cancer.
Journal of Ovarian Research,
Год журнала:
2023,
Номер
16(1)
Опубликована: Сен. 6, 2023
Abstract
Tumor-associated
lncRNAs
regulated
by
epigenetic
modification
switches
mediate
immune
escape
and
chemoresistance
in
ovarian
cancer
(OC).
However,
the
underlying
mechanisms
concrete
targets
have
not
been
systematically
elucidated.
Here,
we
discovered
that
methylation
modifications
played
a
significant
role
regulating
cell
infiltration
sensitizing
OC
to
chemotherapy
modulating
immune-related
(irlncRNAs),
which
represent
tumor
status.
Through
deep
analysis
of
TCGA
database,
prognostic
risk
model
incorporating
four
methylation-related
(mrlncRNAs)
irlncRNAs
was
constructed.
Twenty-one
mrlncRNA/irlncRNA
pairs
were
identified
significantly
related
overall
survival
(OS)
patients.
Subsequently,
selected
construct
signature
predictive
OS
indicative
infiltration,
verified
robustness
an
internal
validation
set.
The
score
independent
factor
for
prognosis,
demonstrated
via
multifactorial
Cox
regression
nomogram.
Moreover,
scores
negatively
expression
CD274,
CTLA4,
ICOS,
LAG3,
PDCD1,
PDCD1LG2
correlated
with
CD8
+
,
CD4
Treg
tumor-infiltrating
cells.
In
addition,
high-risk
associated
higher
IC50
value
cisplatin,
worse
clinical
outcome.
Next,
competing
endogenous
RNA
(ceRNA)
network
signaling
pathway
controlling
T
cells
explored
based
on
lncRNA
model,
suggested
potential
therapeutic
target
immunotherapy.
Overall,
this
study
constructed
pairing
mrlncRNAs
revealed
critical
FTO/RP5-991G20.1/hsa-miR-1976/MEIS1
function
enhancing
anticancer
therapy.
BMC Medical Genomics,
Год журнала:
2024,
Номер
17(1)
Опубликована: Фев. 12, 2024
Abstract
Background
We
aimed
to
investigate
the
involvement
of
long
non-coding
RNA
(lncRNA)
in
bacterial
and
viral
meningitis
children.
Methods
The
peripheral
blood
five
patients,
samples,
healthy
individuals
were
collected
for
sequencing.
Then,
differentially
expressed
lncRNA
mRNA
detected
vs.
controls,
patients.
Besides,
co-expression
competing
endogenous
(ceRNA)
networks
constructed.
Receiver
operating
characteristic
curve
(ROC)
analysis
was
performed.
Results
Compared
with
control
group,
2
lncRNAs
32
mRNAs
identified
115
54
meningitis.
meningitis,
165
765
31
specific
53
function
enrichment
results
indicated
that
these
involved
innate
immune
response,
inflammatory
system
process.
A
total
8
1401
relationships
respectively
found
groups.
ceRNA
contained
1
lncRNA-mRNA
pair
4
miRNA-mRNA
pairs
group.
GPR68
KIF5C,
analysis,
had
an
area
under
(AUC)
1.00,
while
AUC
OR52K2
CCR5
is
0.883
0.698,
respectively.
Conclusions
Our
research
first
profile
children
may
provide
new
insight
into
understanding
regulatory
mechanisms.
