Gingerenone A Attenuates Ulcerative Colitis via Targeting IL‐17RA to Inhibit Inflammation and Restore Intestinal Barrier Function

Advanced Science, Год журнала: 2024, Номер 11(28)

Опубликована: Апрель 19, 2024

Abstract Ulcerative colitis (UC) is a complicated and recurrent intestinal disease. Currently available drugs for UC treatment are scarce, therefore, novel therapeutic the urgently to be developed. Gingerenone A (GA) phenolic compound known its anti‐inflammatory effect, but effect on remains unknown. Here, it shown that GA protects mice against UC, which closely associated with inhibiting mucosal inflammation enhancing barrier integrity in vivo vitro. Of note, RNA sequencing analysis demonstrates an evident correlation IL‐17 signaling pathway after treatment, this further corroborated by Western blot. Mechanistically, directly interacts IL‐17RA protein through pull‐down, surface plasmon resonance molecular dynamics simulation. Importantly, lentivirus‐mediated IL‐17RA/Act1 knock‐down or co‐treatment brodalumab/ixekizumab significantly impairs protective effects of DSS‐induced dysfunction, suggesting critical role GA‐mediated protection UC. Overall, these results indicate effective agent mainly direct binding inhibit inflammatory activation.

Язык: Английский

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CircRNA‐associated ceRNA regulatory networks as emerging mechanisms governing the development and biophysiopathology of epilepsy

CNS Neuroscience & Therapeutics, Год журнала: 2024, Номер 30(4)

Опубликована: Апрель 1, 2024

Abstract The etiology of epilepsy is ascribed to the synchronized aberrant neuronal activity within brain. Circular RNAs (circRNAs), a class non‐coding characterized by their circular structures and covalent linkage, exert substantial influence on this phenomenon. CircRNAs possess stereotyped replication, transience, repetitiveness, paroxysm. Additionally, MicroRNA (miRNA) plays crucial role in regulation diverse pathological processes, including epilepsy. CircRNA particular significance due its ability function as competing endogenous RNA, thereby sequestering or inhibiting miRNA through binding target mRNA. Our review primarily concentrates elucidating functional roles, well underlying mechanisms, circRNA–miRNA–mRNA networks it explores potential utility these for early detection therapeutic intervention.

Язык: Английский

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tsRNA-GlyGCC promotes colorectal cancer progression and 5-FU resistance by regulating SPIB

Journal of Experimental & Clinical Cancer Research, Год журнала: 2024, Номер 43(1)

Опубликована: Авг. 17, 2024

tRNA-derived small RNAs (tsRNAs) are newly discovered non-coding RNA, which generated from tRNAs and reported to participate in several biological processes diseases, especially cancer; however, the mechanism of tsRNA involvement colorectal cancer (CRC) 5-fluorouracil (5-FU) is still unclear.

Язык: Английский

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Non-coding RNAs as mediators of epithelial - Mesenchymal transition (EMT) in metastatic colorectal cancers

Cellular Signalling, Год журнала: 2025, Номер 127, С. 111605 - 111605

Опубликована: Янв. 20, 2025

Язык: Английский

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Unveiling prognostic value of JAK/STAT signaling pathway related genes in colorectal cancer: a study of Mendelian randomization analysis

Infectious Agents and Cancer, Год журнала: 2025, Номер 20(1)

Опубликована: Фев. 7, 2025

Colorectal cancer (CRC) ranks among the frequently occurring malignant neoplasms affecting gastrointestinal tract. This study aimed to explore JAK-STAT signaling pathway related genes in CRC and establish a new prognostic model. The data set used this is from public database. JAK-STAT-differentially expressed (DEGs) were identified through differential expression analysis weighted gene co-expression network (WGCNA). Prognostic selected JAK-STAT-DEGs Mendelian randomization (MR), univariate Cox regression, least absolute shrinkage selection operator (LASSO) analyses. expressions of verified by RT-qPCR. Then, risk model was built validated GSE39582. Independent factors screened underlying scores different clinical indicators, resulting construction nomogram. Additionally, immune infiltration, checkpoint inhibitors analyses enrichment (GSEA) carried out. 3,668 obtained intersection 5826 CRC-DEGs 9766 key module genes. Five (ANK3, F5, FAM50B, KLHL35, MPP2), their significantly between control groups. A constructed according In addition, nomogram exhibited superior predictive accuracy for CRC. Furthermore, results indicated notable positive correlation score (R = 0.486), stromal 0.309), ESTIMATE 0.422). Immune inhibitor ADORA2A (Cor 0.483263) strongest with score. And MPP2 most potent activating influence on cell cycle pathway, whereas ANK3 demonstrated significant inhibitory effect within apoptosis pathway. validated, which possessed an potential patients' prognosis could potentially enhance tailored guidance immunotherapy.

Язык: Английский

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Long non-coding RNAs (lncRNAs) in cancer development: new insight from STAT3 signaling pathway to immune evasion

Clinical and Experimental Medicine, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 11, 2025

Overcoming cancer and enhancing patient survival are becoming increasingly challenging due to the uncontrolled growth metastasis of colorectal cells. In order provide effective treatment minimize malignancy cells, it is necessary understand how complex signaling networks contribute their invasion proliferation. The signal transducer activator transcription 3 (STAT3) a promising target its involvement in various cellular functions, including apoptosis, immunosuppression, cell invasion, migration, Dysregulation STAT3 associated with diseases, particularly cancer. Long non-coding RNAs (lncRNAs), subset RNAs, essential for progression, CRC as they regulate key pathways such gene regulation at epigenetic, transcriptional, post-transcriptional levels. Moreover, lncRNAs have function regulating immune cells through STAT3. this study, we comprehensively reviewed regulatory roles different on mutual effects pathway aspects carcinogenesis, proliferation, metastasis, drug resistance, angiogenesis. investigate lncRNA/STAT3 axis that play critical role tumor microenvironment.

Язык: Английский

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Long non-coding RNA OSTM1-AS1 promotes renal cell carcinoma progression by sponging miR-491-5p and upregulating MMP-9

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 2, 2025

Язык: Английский

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LncRNA FAM30A Suppresses Proliferation and Metastasis of Colorectal Carcinoma by Blocking the JAK–STAT Signalling

Journal of Cellular and Molecular Medicine, Год журнала: 2025, Номер 29(4)

Опубликована: Фев. 1, 2025

ABSTRACT Colorectal carcinoma (CRC) poses a serious risk to global human health. Long non‐coding RNAs (LncRNAs) play an important role in the pathogenesis of CRC. There is scarcity data about newly identified lncRNA, FAM30A. Our major objective investigate FAM30A process Gene expression and correlated clinical information were retrieved downloaded from public databases identify differentially expressed genes linked The was samples CRC cell lines using via Quantitative Real‐time Polymerase Chain Reaction (qPCR) assay also. survival significance determined R package “survival.” Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis performed FAM30A‐related signalling pathway. levels proteins by western blot assay. effect on biological behaviours evaluated function experiments. down‐regulated based database. had lower lines. Low positively related poor prognosis patients. After overexpressed, proliferation, invasion, migration abilities cells decreased, rate apoptosis increased. Furthermore, overexpression could block JAK–STAT signalling. suppresses proliferative, invasive, migratory through blocking Thus, it can be novel biomarker prognosis.

Язык: Английский

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Targeting the JAK-STAT pathway in colorectal cancer: mechanisms, clinical implications, and therapeutic potential

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Ноя. 26, 2024

Colorectal cancer (CRC) remains one of the most prevalent and fatal malignancies worldwide, consistently ranking among top three in terms incidence mortality. Despite notable advancements early detection therapeutic interventions, survival outcomes for advanced-stage CRC are still dismal, largely due to issues such as drug resistance metastasis. Recent research has increasingly implicated JAK-STAT signaling pathway a pivotal contributor pathogenesis. This evolutionarily conserved plays key role transmitting extracellular signals nucleus, thereby modulating gene expression involved numerous fundamental biological processes. In CRC, dysregulation is frequently observed strongly associated with tumor progression, including processes cellular proliferation, apoptosis, metastasis, immune evasion, sustenance stem cells. Given its integral advancement, gained recognition viable target. Extensive evidence from preclinical clinical models supports efficacy safety targeting components pathway, presenting new possibilities patients particularly addressing enhancing treatment outcomes. review offers detailed exploration focusing on regulatory mechanisms CRC-related malignancies. Moreover, it examines association between protein expression, features, prognosis, potential management.

Язык: Английский

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Gingerenone A induces ferroptosis in colorectal cancer via targeting suppression of SLC7A11 signaling pathway

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 180, С. 117529 - 117529

Опубликована: Окт. 10, 2024

Язык: Английский

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