Frontiers in Genetics,
Год журнала:
2024,
Номер
15
Опубликована: Апрель 19, 2024
Background:
In
sub-Saharan
Africa,
80%
of
poultry
production
is
on
smallholder
village
farms,
where
chickens
are
typically
reared
outdoors
in
free-ranging
conditions.
There
limited
knowledge
chickens’
phenotypic
characteristics
and
genetics
under
these
Objective:
The
present
a
large-scale
study
set
out
to
phenotypically
characterise
the
performance
tropically
adapted
commercial
typical
farm
conditions,
examine
genetic
profile
chicken
phenotypes
associated
with
growth,
meat
production,
immunity,
survival.
Methods:
A
total
2,573
T451A
dual-purpose
Sasso
kept
emulated
conditions
at
facility
International
Livestock
Research
Institute
Addis
Ababa,
Ethiopia,
were
included
study.
raised
five
equally
sized
batches
individually
monitored
phenotyped
from
age
56
days
for
8
weeks.
Individual
data
collected
weekly
body
weight,
growth
rate,
breast
weight
slaughter,
Newcastle
Disease
Virus
(NDV)
titres
intestinal
Immunoglobulin
(IgA)
levels
recorded
beginning
end
period
study,
survival
rate
during
same
period.
Genotyping
by
sequencing
was
performed
all
using
low-coverage
imputation
approach.
Chicken
genotypes
combined
genomic
association
analyses.
Results:
We
discovered
that
diverse,
extensive
variance
observed
traits.
Batch
number
sex
significantly
affected
studied
phenotypes.
Following
quality
assurance,
consisted
2.9
million
Single
Nucleotide
Polymorphism
markers
used
Results
revealed
largely
polygenic
mode
control
Nevertheless,
15
distinct
identified
carcass
traits,
NDV
titres,
IgA
levels,
These
located
regions
harbouring
relevant
annotated
genes.
Conclusion:
suggest
amenable
improvement
may
inform
selective
breeding
programmes
enhanced
productivity
Africa.
Abstract
Ulcerative
colitis
(UC)
is
a
complicated
and
recurrent
intestinal
disease.
Currently
available
drugs
for
UC
treatment
are
scarce,
therefore,
novel
therapeutic
the
urgently
to
be
developed.
Gingerenone
A
(GA)
phenolic
compound
known
its
anti‐inflammatory
effect,
but
effect
on
remains
unknown.
Here,
it
shown
that
GA
protects
mice
against
UC,
which
closely
associated
with
inhibiting
mucosal
inflammation
enhancing
barrier
integrity
in
vivo
vitro.
Of
note,
RNA
sequencing
analysis
demonstrates
an
evident
correlation
IL‐17
signaling
pathway
after
treatment,
this
further
corroborated
by
Western
blot.
Mechanistically,
directly
interacts
IL‐17RA
protein
through
pull‐down,
surface
plasmon
resonance
molecular
dynamics
simulation.
Importantly,
lentivirus‐mediated
IL‐17RA/Act1
knock‐down
or
co‐treatment
brodalumab/ixekizumab
significantly
impairs
protective
effects
of
DSS‐induced
dysfunction,
suggesting
critical
role
GA‐mediated
protection
UC.
Overall,
these
results
indicate
effective
agent
mainly
direct
binding
inhibit
inflammatory
activation.
CNS Neuroscience & Therapeutics,
Год журнала:
2024,
Номер
30(4)
Опубликована: Апрель 1, 2024
Abstract
The
etiology
of
epilepsy
is
ascribed
to
the
synchronized
aberrant
neuronal
activity
within
brain.
Circular
RNAs
(circRNAs),
a
class
non‐coding
characterized
by
their
circular
structures
and
covalent
linkage,
exert
substantial
influence
on
this
phenomenon.
CircRNAs
possess
stereotyped
replication,
transience,
repetitiveness,
paroxysm.
Additionally,
MicroRNA
(miRNA)
plays
crucial
role
in
regulation
diverse
pathological
processes,
including
epilepsy.
CircRNA
particular
significance
due
its
ability
function
as
competing
endogenous
RNA,
thereby
sequestering
or
inhibiting
miRNA
through
binding
target
mRNA.
Our
review
primarily
concentrates
elucidating
functional
roles,
well
underlying
mechanisms,
circRNA–miRNA–mRNA
networks
it
explores
potential
utility
these
for
early
detection
therapeutic
intervention.
Journal of Experimental & Clinical Cancer Research,
Год журнала:
2024,
Номер
43(1)
Опубликована: Авг. 17, 2024
tRNA-derived
small
RNAs
(tsRNAs)
are
newly
discovered
non-coding
RNA,
which
generated
from
tRNAs
and
reported
to
participate
in
several
biological
processes
diseases,
especially
cancer;
however,
the
mechanism
of
tsRNA
involvement
colorectal
cancer
(CRC)
5-fluorouracil
(5-FU)
is
still
unclear.
Infectious Agents and Cancer,
Год журнала:
2025,
Номер
20(1)
Опубликована: Фев. 7, 2025
Colorectal
cancer
(CRC)
ranks
among
the
frequently
occurring
malignant
neoplasms
affecting
gastrointestinal
tract.
This
study
aimed
to
explore
JAK-STAT
signaling
pathway
related
genes
in
CRC
and
establish
a
new
prognostic
model.
The
data
set
used
this
is
from
public
database.
JAK-STAT-differentially
expressed
(DEGs)
were
identified
through
differential
expression
analysis
weighted
gene
co-expression
network
(WGCNA).
Prognostic
selected
JAK-STAT-DEGs
Mendelian
randomization
(MR),
univariate
Cox
regression,
least
absolute
shrinkage
selection
operator
(LASSO)
analyses.
expressions
of
verified
by
RT-qPCR.
Then,
risk
model
was
built
validated
GSE39582.
Independent
factors
screened
underlying
scores
different
clinical
indicators,
resulting
construction
nomogram.
Additionally,
immune
infiltration,
checkpoint
inhibitors
analyses
enrichment
(GSEA)
carried
out.
3,668
obtained
intersection
5826
CRC-DEGs
9766
key
module
genes.
Five
(ANK3,
F5,
FAM50B,
KLHL35,
MPP2),
their
significantly
between
control
groups.
A
constructed
according
In
addition,
nomogram
exhibited
superior
predictive
accuracy
for
CRC.
Furthermore,
results
indicated
notable
positive
correlation
score
(R
=
0.486),
stromal
0.309),
ESTIMATE
0.422).
Immune
inhibitor
ADORA2A
(Cor
0.483263)
strongest
with
score.
And
MPP2
most
potent
activating
influence
on
cell
cycle
pathway,
whereas
ANK3
demonstrated
significant
inhibitory
effect
within
apoptosis
pathway.
validated,
which
possessed
an
potential
patients'
prognosis
could
potentially
enhance
tailored
guidance
immunotherapy.
Clinical and Experimental Medicine,
Год журнала:
2025,
Номер
25(1)
Опубликована: Фев. 11, 2025
Overcoming
cancer
and
enhancing
patient
survival
are
becoming
increasingly
challenging
due
to
the
uncontrolled
growth
metastasis
of
colorectal
cells.
In
order
provide
effective
treatment
minimize
malignancy
cells,
it
is
necessary
understand
how
complex
signaling
networks
contribute
their
invasion
proliferation.
The
signal
transducer
activator
transcription
3
(STAT3)
a
promising
target
its
involvement
in
various
cellular
functions,
including
apoptosis,
immunosuppression,
cell
invasion,
migration,
Dysregulation
STAT3
associated
with
diseases,
particularly
cancer.
Long
non-coding
RNAs
(lncRNAs),
subset
RNAs,
essential
for
progression,
CRC
as
they
regulate
key
pathways
such
gene
regulation
at
epigenetic,
transcriptional,
post-transcriptional
levels.
Moreover,
lncRNAs
have
function
regulating
immune
cells
through
STAT3.
this
study,
we
comprehensively
reviewed
regulatory
roles
different
on
mutual
effects
pathway
aspects
carcinogenesis,
proliferation,
metastasis,
drug
resistance,
angiogenesis.
investigate
lncRNA/STAT3
axis
that
play
critical
role
tumor
microenvironment.
Journal of Cellular and Molecular Medicine,
Год журнала:
2025,
Номер
29(4)
Опубликована: Фев. 1, 2025
ABSTRACT
Colorectal
carcinoma
(CRC)
poses
a
serious
risk
to
global
human
health.
Long
non‐coding
RNAs
(LncRNAs)
play
an
important
role
in
the
pathogenesis
of
CRC.
There
is
scarcity
data
about
newly
identified
lncRNA,
FAM30A.
Our
major
objective
investigate
FAM30A
process
Gene
expression
and
correlated
clinical
information
were
retrieved
downloaded
from
public
databases
identify
differentially
expressed
genes
linked
The
was
samples
CRC
cell
lines
using
via
Quantitative
Real‐time
Polymerase
Chain
Reaction
(qPCR)
assay
also.
survival
significance
determined
R
package
“survival.”
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
enrichment
analysis
performed
FAM30A‐related
signalling
pathway.
levels
proteins
by
western
blot
assay.
effect
on
biological
behaviours
evaluated
function
experiments.
down‐regulated
based
database.
had
lower
lines.
Low
positively
related
poor
prognosis
patients.
After
overexpressed,
proliferation,
invasion,
migration
abilities
cells
decreased,
rate
apoptosis
increased.
Furthermore,
overexpression
could
block
JAK–STAT
signalling.
suppresses
proliferative,
invasive,
migratory
through
blocking
Thus,
it
can
be
novel
biomarker
prognosis.
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Ноя. 26, 2024
Colorectal
cancer
(CRC)
remains
one
of
the
most
prevalent
and
fatal
malignancies
worldwide,
consistently
ranking
among
top
three
in
terms
incidence
mortality.
Despite
notable
advancements
early
detection
therapeutic
interventions,
survival
outcomes
for
advanced-stage
CRC
are
still
dismal,
largely
due
to
issues
such
as
drug
resistance
metastasis.
Recent
research
has
increasingly
implicated
JAK-STAT
signaling
pathway
a
pivotal
contributor
pathogenesis.
This
evolutionarily
conserved
plays
key
role
transmitting
extracellular
signals
nucleus,
thereby
modulating
gene
expression
involved
numerous
fundamental
biological
processes.
In
CRC,
dysregulation
is
frequently
observed
strongly
associated
with
tumor
progression,
including
processes
cellular
proliferation,
apoptosis,
metastasis,
immune
evasion,
sustenance
stem
cells.
Given
its
integral
advancement,
gained
recognition
viable
target.
Extensive
evidence
from
preclinical
clinical
models
supports
efficacy
safety
targeting
components
pathway,
presenting
new
possibilities
patients
particularly
addressing
enhancing
treatment
outcomes.
review
offers
detailed
exploration
focusing
on
regulatory
mechanisms
CRC-related
malignancies.
Moreover,
it
examines
association
between
protein
expression,
features,
prognosis,
potential
management.
American Journal of Reproductive Immunology,
Год журнала:
2024,
Номер
92(2)
Опубликована: Авг. 1, 2024
Long
non-coding
RNAs
(lncRNAs)
play
crucial
roles
in
cellular
processes,
with
dysregulation
implicated
various
diseases,
including
cancers.
The
lncRNA
TPT1-AS1
(TPT1
Antisense
RNA
1)
promotes
tumor
progression
several
cancers,
ovarian
cancer
(OC),
but
its
influence
on
ferroptosis
and
interaction
other
proteins
remains
underexplored.