Purification, structural characterization, and immunomodulatory activity of two polysaccharides from Portulaca oleracea L.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 264, С. 130508 - 130508

Опубликована: Фев. 29, 2024

Язык: Английский

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Effects of Fucoidan Isolated From Laminaria japonica on Immune Response and Gut Microbiota in Cyclophosphamide-Treated Mice

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Май 19, 2022

The effects of Laminaria japonica fucoidan (LF) on immune regulation and intestinal microflora in cyclophosphamide (CTX)-treated mice were investigated this work. Results indicated that LF significantly enhanced the spleen thymus indices, promoted lymphocyte peritoneal macrophages proliferation, increased immune-related cytokines production serum. Moreover, could regulate flora composition, increasing abundance Lactobacillaceae Alistipes , inhibiting Erysipelotrichia Turicibacter Romboutsia Peptostreptococcaceae Faecalibaculum . These results positively correlated with characteristics. Overall, be useful as a new potential strategy to mitigate CTX immunosuppression microbiota disorders.

Язык: Английский

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Modulating Effects of Turmeric Polysaccharides on Immune Response and Gut Microbiota in Cyclophosphamide-Treated Mice

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(7), С. 3469 - 3482

Опубликована: Фев. 8, 2024

Turmeric, a traditional medicinal herb, is commonly used as dietary and functional ingredient. This study aimed to investigate the effect of turmeric polysaccharides (TPs) on intestinal immunity gut microbiota in cyclophosphamide (Cy)-induced immunosuppressed BALB/c mice. We verified that oral administration TPs-0 TPs-3 (200 400 mg/kg, bw) improved thymus spleen indexes, increased whole blood immune cells (WBC) lymph count index, stimulated secretion serum immunoglobulin IgG. More importantly, could repair damage reduce inflammation. The specific mechanism ameliorating pathological damage, promoting CD4+ T cell secretion, regulating expression related cytokines, reducing level critical proteins NF-κB/iNOS pathway. Interestingly, intake significantly content short-chain fatty acids (SCFAs). Moreover, relieved disorder via proliferation abundance Lactobacillus Bacteroides inhibition Staphylococcus. Cumulatively, our suggests can relieve by repairing barrier flora disorders. TPs have potential applications for enhancing food.

Язык: Английский

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In vitro and in vivo immunomodulatory activity of acetylated polysaccharides from Cyclocarya paliurus leaves

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 259, С. 129174 - 129174

Опубликована: Янв. 4, 2024

Язык: Английский

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Carboxymethylated Abrus cantoniensis polysaccharide prevents CTX-induced immunosuppression and intestinal damage by regulating intestinal flora and butyric acid content

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 261, С. 129590 - 129590

Опубликована: Янв. 23, 2024

Язык: Английский

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Integrated serum pharmacochemistry and network pharmacology to explore the mechanism of Yi-Shan-Hong formula in alleviating chronic liver injury

Phytomedicine, Год журнала: 2024, Номер 128, С. 155439 - 155439

Опубликована: Фев. 9, 2024

Язык: Английский

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Mulberry leaf polysaccharide improves cyclophosphamide-induced growth inhibition and intestinal damage in chicks by modulating intestinal flora, enhancing immune regulation and antioxidant capacity

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Март 21, 2024

Polysaccharides are generally considered to have immune enhancing functions, and mulberry leaf polysaccharide is the main active substance in leaves, while there few studies on whether (MLP) has an effect immunosuppression intestinal damage caused by cyclophosphamide (CTX), we investigated MLP ameliorative CTX. A total of 210 1-day-old Mahuang cocks were selected for this experiment. Were equally divided into six groups used evaluate MLP. Our results showed that significantly enhanced growth performance chicks elevated secretion cytokines (IL-1β, IL-10, IL-6, TNF-α, IFN-γ), immunoglobulins antioxidant enzymes serum immunosuppressed chicks. It attenuated jejunal expression tight junction proteins Claudin1, Zo-1 MUC2, which protected health. activated TLR4-MyD88-NF-κB pathway TLR4, MyD88 NF-κB, served protect intestine. 16S rDNA gene high-throughput sequencing increased species richness, restored CTX-induced gut microbiome imbalance, abundance probiotic bacteria gut. improves cyclophosphamide-induced inhibition modulating flora regulation capacity. In conclusion, study provides a scientific basis as enhancer regulate chick mucosal damage.

Язык: Английский

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Antitumor effects of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg via regulation of intestinal flora and enhancing immunomodulatory effects in vivo

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Окт. 28, 2022

Tetrastigma hemsleyanum Diels et Gilg is a traditional Chinese herbal medicine with high medicinal value, and antitumor, antioxidant anti-inflammatory biological activities. However, while several studies have focused on flavonoids in tubers, there are few the enhanced immune effect of polysaccharides (THP). In this study, we evaluated antitumor THP lung tumor model explored mechanism activity through intestinal flora. addition, cyclophosphamide (CTX)-induced immunosuppression was used to declare immunomodulatory immunosuppressive state induced by drugs. The results showed that increased content ileum secreted immunoglobulin A (SIgA) cecum short-chain fatty acids (SCFAs) improved microbial community diversity, regulating relative abundance dominant microbiota flora from phylum level genus level, recovering microflora disorder caused tumors. Additionally, can increase organ indices improve atrophy. upregulate routine blood counts stimulate production serum cytokines. also promoted macrophage phagocytic index, NK-cell activation, complement (IgG, IgA, IgM) levels. detection Splenic lymphocyte proliferation T subsets sideways reflects restore CTX-induced inhibition mice. conclusion, study suggests effectively achieve effects, regulate gut Therefore, enhance capacity provide novel antineoplastic adjuvant agents.

Язык: Английский

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Progress in the metabolic kinetics and health benefits of functional polysaccharides from plants, animals and microbes: A review

Carbohydrate Polymer Technologies and Applications, Год журнала: 2024, Номер 7, С. 100526 - 100526

Опубликована: Июнь 1, 2024

Functional polysaccharides are characterized by functional and structural biomolecules, which play a dual role, both as nutrient molecules providing energy signalling involved in intercellular communications. There increasing evidences showing that possess beneficial effects for treating chronic diseases, such cancer, cardiovascular disease, etc, but its mechanism of action remains imprecision. Additionally, due to the limitations precision detection methods complexity their structures, metabolic kinetics behaviours or bioavailability polysaccharides, interaction between it entering systemic circulation target cells have been conundrum faced researcher. This review summarized latest advances efficacy diseases structure-activity relationship, absorption behaviours, specific cellular receptors so understand mechanisms bioactivities. is expected provide new insights paves way application.

Язык: Английский

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Modulation of cyclophosphamide-induced immunosuppression and intestinal flora in broiler by deep eutectic solvent extracted polysaccharides of Acanthopanax senticosus

Frontiers in Veterinary Science, Год журнала: 2024, Номер 11

Опубликована: Май 27, 2024

The aim of this experiment was to investigate the modulation effect Acanthopanax senticosus polysaccharide (ASPS-PD) extracted with deep eutectic solvent on cyclophosphamide-induced immunosuppression in broilers and its gut microbiota broilers. 108 one-day-old were divided into six groups, including control group, Cyclophosphamide (CY) model ASPS-PD ASPA-PD high low dose groups Astragalus group. Body weight, feed intake, conversion ratio, immune organ index at 7, 14, 21 days determined; IL-2, IFN-γ, lgG1 levels determined by enzyme-linked immunosorbent assay (ELISA); Broiler caeca feces analyzed amplification 16S rRNA sequencing. results showed that can restore growth performance, increase improve serum cytokine IL-2 IFN-γ immunoglobulin CY-treated analysis cecum flora promote proliferation beneficial bacteria reduce number harmful bacteria, regulating intestinal flora. Therefore, may be a potential novel immunomodulator ameliorate CY-induced dysregulation broiler.

Язык: Английский

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