Applied Biochemistry and Biotechnology, Год журнала: 2024, Номер unknown
Опубликована: Авг. 3, 2024
Язык: Английский
Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)
Опубликована: Сен. 20, 2024
Язык: Английский
Процитировано
44
Discover Oncology, Год журнала: 2024, Номер 15(1)
Опубликована: Янв. 31, 2024
Abstract The global prevalence of head and neck malignancies positions them as the sixth most common form cancer, with squamous cell carcinoma (HNSCC) representing predominant histological subtype. Despite advancements in multidisciplinary approaches molecular targeted therapies, therapeutic outcomes for HNSCC have only marginally improved, particularly cases recurrent or metastatic (R/MHNSCC). This situation underscores critical necessity development innovative strategies. Such strategies are essential not to enhance efficacy treatment but also minimize incidence associated complications, thus improving overall patient prognosis. Cancer immunotherapy represents a cutting-edge cancer that leverages immune system targeting destroying cells. It's applied multiple cancers, including melanoma lung offering precision, adaptability, potential long-lasting remission through memory. It is observed while patients responsive often experience prolonged benefits, limited subset demonstrates such responsiveness. Additionally, significant clinical challenges remain, resistance immunotherapy. biological characteristics, dynamic inhibitory changes, heterogeneity tumor microenvironment (TME) play roles its pathogenesis, evasion, resistance. review aims elucidate functions mechanisms anti-tumor cells extracellular components within TME. introduces several immunosuppressive agents commonly utilized immunotherapy, examines factors influencing effectiveness these treatments, provides comprehensive summary immunotherapeutic relevant HNSCC.
Язык: Английский
Процитировано
10
Journal of Controlled Release, Год журнала: 2024, Номер 372, С. 682 - 698
Опубликована: Июль 2, 2024
Язык: Английский
Процитировано
10
Molecular Medicine, Год журнала: 2025, Номер 31(1)
Опубликована: Янв. 23, 2025
Abstract Background Acute rejection (AR) is one of the significant factors contributing to poor prognosis in patients following kidney transplantation. Neutrophils are main cause early host-induced tissue injury. This paper intends investigate possible mechanisms neutrophil involvement acute renal Methods Samples were analyzed for their relationship with immune cells using CIBERSORT. WGCNA was used identify modules high relevance neutrophils and hub genes extracted. The effect on function after blocking formyl peptide receptor 1 (FPR1) tested vitro experiments. effects FPR1 as well vivo constructing a mouse transplant model. Results proportion higher AR group than non-rejection group, identified an important gene regulation transplantation by neutrophils. At cellular level, inhibited activation ERK1/2 pathway, decreased ferrous ion content, affected expression iron metabolism-related proteins, suppressed formation NETs. In model transplantation, blockade graft infiltration NETs content. Meanwhile, attenuated injury during rejection. Conclusion study found that might be molecule involved explored between neutrophils, provided potential treatment methods clinical practice.
Язык: Английский
Процитировано
2
Frontiers in Immunology, Год журнала: 2023, Номер 14
Опубликована: Июль 13, 2023
Background Neutrophil extracellular traps (NETs) have been shown to play a pivotal role in promoting metastasis and immune escape hepatocellular carcinoma (HCC). Therefore, noninvasive tests detect the formation of NETs tumors can significant implications for treatment prognoses patients. Here, we sought develop validate computed tomography (CT)-based radiomics model predict gene expression profiles that regulate HCC. Methods This study included 1133 HCC patients from five retrospective cohorts. Based on mRNA levels 69 biomarkers correlated with NET formation, 6-gene score (NETs score, NETS) was constructed cohort 1 TCIA database (n=52) validated 2 (n=232) ICGC 3 (n=365) TCGA database. And then based features CT images, signature (RNETS) developed NETS status (high- or low-NETS). We further employed two cohorts Nanfang Hospital (Guangzhou, China) evaluate predictive power RNETS predicting prognosis 4 (n=347) responses PD-1 inhibitor 5 (n=137). Results For NETS, 1, area under curve (AUC) values 2, 3-year overall survival (OS) were 0.836, 0.879, 0.902, respectively. The low-NETS associated better higher cell infiltration. yielded an AUC value 0.853 distinguishing between high-NETS low-RNETS significantly longer time ( P <0.001). Notably, competent disease-free (DFS) OS In 5, found be independent risk factor progression-free (PFS) addition, objective response rate treated group (27.8%) than high-RNETS (10.8%). Conclusions revealed as biomarker could effectively immunotherapy
Язык: Английский
Процитировано
22
Molecular Cancer, Год журнала: 2023, Номер 22(1)
Опубликована: Апрель 6, 2023
Abstract The development of head and neck squamous cell carcinoma (HNSCC) is a multi-step process, its survival depends on complex tumor ecosystem, which not only promotes growth but also helps to protect cells from immune surveillance. With the advances existing technologies emerging models for ecosystem research, evidence cell-cell interplay increasing. Herein, we discuss recent in understanding interaction between cells, major components HNSCC summarize mechanisms how biological abiotic factors affect ecosystem. In addition, review ecological treatment strategy based studies.
Язык: Английский
Процитировано
21
Frontiers in Pharmacology, Год журнала: 2025, Номер 15
Опубликована: Янв. 27, 2025
Osteosarcoma is the most common primary bone cancer with a high propensity for local invasion and metastasis. An increasing number of research studies show that telomeres play an important role in occurrence development cancer. Thus, we established telomere-related signature osteosarcoma to comprehensively evaluate pathogenic roles this disease. The data on were collected from TARGET Gene Expression Omnibus databases. First, constructed using univariate LASSO Cox regression analyses. Subsequently, analyzed prognostic value, functional annotation, immune microenvironment, cell communication patterns via comprehensive bioinformatics Cell proliferation was CCK-8 assay, migration capabilities evaluated Transwell assay. Based SP110, HHAT, TUBB, MORC4, TERT, PPARG, MAP3K5, PAGE5, MAP7, CAMK1G, built patients. could effectively predict prognosis patients TELscore group exhibited poor prognosis. In addition, demonstrated predictive value microenvironment drug sensitivity osteosarcoma. Finally, discovered significant reduction MAP7 expression cells, low had Moreover, overexpression significantly reduced proliferation, ability migration, cells. A patients, offering values into prognosis, sensitivity. might serve as marker
Язык: Английский
Процитировано
1
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Фев. 13, 2025
Язык: Английский
Процитировано
1
Journal of Inflammation Research, Год журнала: 2023, Номер Volume 16, С. 3419 - 3436
Опубликована: Авг. 1, 2023
Neutrophil extracellular traps (NETs) released by neutrophils are crucial for cancer development, metastasis, and can indicate gastric (GC) patients' prognosis. This study reveals the relevance of NETs-related genes to GC through transcriptome analysis. We obtained sequencing data from UCSC Xena screened prognostic GEPIA2 database. The signature NETs was subsequently created using LASSO-Cox regression. clinical value model further explored nomogram externally validated GEO After that, we employed GO, KEGG, GSEA enrichment analyses evaluate bio-functional related pathways. Additionally, ESTIMATE, MCP counter, ssGSEA scores were used investigate immunological microenvironment patients. Finally, in external cohort, neutrophil elastase (NE)-DNA complexes measured ELISA, NE-DNA investigated Cox Seven NETs-associated (PDE4B, CD93, CTSG, IL6, ELANE, KCNJ15, CRISPLD2) filtered establish participated building nomogram. In comparison high-risk group, overall survival (OS) much longer low-risk group (P=0.005). validation cohort demonstrated acceptable predictive ability enriched biological features such as matrix organization, epithelial-mesenchymal transition inflammatory response. Moreover, there substantial differences immune cell infiltration across different risk groups (p<0.001), especially having more cells that engaged antigen presentation process associated functions. levels shown be an independent factor affecting OS prognosis (p=0.006). Overall, this research identified a novel based on seven predict identify tumor GC. And high level may critical poor with NETs.
Язык: Английский
Процитировано
16
BMC Musculoskeletal Disorders, Год журнала: 2025, Номер 26(1)
Опубликована: Март 31, 2025
Neutrophil extracellular trap (NET) has been implicated in cancer progression and metastasis. Nevertheless, the role of NET-related gene, formyl peptide receptor 1 (FPR1), osteosarcoma (OS) remains largely unexplored. This study aimed to investigate prognostic significance biological function FPR1 OS. The least absolute shrinkage selection operator (LASSO) algorithm was employed construct a model utilizing OS datasets from TARGET GEO (GSE21257) databases. scRNA-seq dataset GSE162454 then used for verifying at single-cell resolution. Next, survival analysis multivariate cox regression were performed evaluate value patients. CIBERSORT conducted relationship between levels immune cell abundance. Subsequently, explored through CCK-8, transwell assays lines. A signature NET score, comprising four genes (TNFRSF10C, FPR1, BST1 SELPLG), constructed predict prognosis outcomes patients high-NET score group markedly worse than that low-NET group. Meanwhile, single resolution, cells progressively evolved into tumors with elevated scores. Furthermore, reduced when compared normal osteoblast cells, overexpression notably suppressed viability, migration invasion. Additionally, exhibiting high demonstrated favorable overall survival. Moreover, these also had higher proportion M1 macrophages lower M0 macrophages. Collectively, our indicates gene is closely related tumor progression, infiltration
Язык: Английский
Процитировано
0