Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide DOI Open Access

Daria M. Yurkina,

Elena A. Romanova,

K.A. Shcherbakov

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(12), С. 6633 - 6633

Опубликована: Июнь 16, 2024

Receptors of cytokines are major regulators the immune response. In this work, we have discovered two new ligands that can activate TNFR1 (tumor necrosis factor receptor 1) receptor. Earlier, found peptide Tag (PGLYRP1) protein designated 17.1 interact with Here, Mts1 (S100A4) interacts a high affinity (Kd = 1.28 × 10-8 M), and complex is cytotoxic to cancer cells on their surface. This induces both apoptosis necroptosis in involvement mitochondria lysosomes cell death signal transduction. Moreover, succeeded locating fragment responsible for protein-peptide interaction, which highly specifically Tag7 2.96 nM). The isolated M7 also forms 17.1, peptide-peptide receptor-dependent death. Molecular docking molecular dynamics experiments show amino acids involved binding may be used peptidomimetics' development. Thus, complexes were created able induce tumor via These results therapy autoimmune diseases.

Язык: Английский

Ferroptosis role in complexity of cell death: unrevealing mechanisms in Parkinson’s disease and therapeutic approaches DOI
Anupam Awasthi,

Kousik Maparu,

Shamsher Singh

и другие.

Inflammopharmacology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

Язык: Английский

Процитировано

4
Nutritional, antioxidant and biological activity characterization of orange peel flour to produce nutraceutical gluten-free muffins DOI Creative Commons
Giusy Rita Caponio, Alessandro Annunziato, Mirco Vacca

и другие.

Food & Function, Год журнала: 2024, Номер 15(16), С. 8459 - 8476

Опубликована: Янв. 1, 2024

Celiac disease – a prevalent food intolerance requires strict adherence to lifelong gluten-free (GF) diet as the only effective treatment.

Язык: Английский

Процитировано

5
Multiple roles of p53 in cancer development: Regulation of tumor microenvironment, m6A modification and diverse cell death mechanisms DOI Creative Commons

Xiangyu Wang,

Jianhua Yang, Wanting Yang

и другие.

Journal of Advanced Research, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

4
Prognostic risk modeling of endometrial cancer using programmed cell death-related genes: a comprehensive machine learning approach DOI Creative Commons
Tianshu Chen, Yuhan Yang, Zhizhong Huang

и другие.

Discover Oncology, Год журнала: 2025, Номер 16(1)

Опубликована: Март 8, 2025

Endometrial cancer represents a significant health challenge, with rising incidence and complex prognostic challenges. This study aimed to develop robust predictive model integrating programmed cell death-related genes advanced machine learning techniques. Utilizing transcriptomic data from TCGA-UCEC GSE119041 datasets, we employed comprehensive approach involving 117 algorithms. Key methodologies included differential gene expression analysis, weighted co-expression network functional enrichment studies, immune landscape evaluation, multi-dimensional risk stratification. We identified 10 critical (PTGIS, TIMP3, SRPX, SNCA, HIC1, BAK1, STXBP2, TRIB3, RTKN2, E2F1) constructed superior performance. The StepCox[forward] + plsRcox algorithm combination demonstrated excellent accuracy (AUC > 0.8). Kaplan–Meier analysis revealed survival differences between high- low-risk groups in both training (HR = 3.37, p < 0.001) validation cohorts 2.05, 0.021). showed strong correlations clinical characteristics, infiltration patterns, potential therapeutic responses. presents novel, endometrial prognosis, molecular insights provide more precise stratification tool translation.

Язык: Английский

Процитировано

0
Cathepsin B induces kidney diseases through different types of programmed cell death DOI Creative Commons
Yunlong Zhao, Yong Zhuang, Jie Shi

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 10, 2025

Cathepsin B (CTSB), a key cysteine protease, plays essential roles in physiological and pathological processes. As research progresses, interest how CTSB triggers different types of programmed cell death (PCD) to induce the onset development diseases is increasing. Several recent studies suggest that PCD mediated by play kidney diseases. In this review, we outline fundamental mechanisms which several discuss function various segments kidney. Moreover, explore possibilities prospects using as therapeutic target for

Язык: Английский

Процитировано

0
Caspase-independent cell death in lung cancer: from mechanisms to clinical applications DOI Creative Commons
Gaurav Gupta,

Vijaya Paul Samuel,

M. M. Rekha

и другие.

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 21, 2025

Abstract Caspase-independent cell death (CICD) has recently become a very important mechanism in lung cancer, particular, to overcome critical failure apoptotic that is common disease progression and treatment failures. The pathways involved CICD span from necroptosis, ferroptosis, mitochondrial dysfunction, autophagy-mediated death. Its potential therapeutic applications have been highlighted. Glutathione peroxidase 4 (GPX4) inhibition-driven ferroptosis drug resistance non-small cancer (NSCLC). In addition, necroptosis involving RIPK1 RIPK3 causes tumor modulation of immune responses the microenvironment (TME). Mitochondrial are for through metabolic redox homeostasis. Ferroptosis amplified by reactive oxygen species (ROS) lipid peroxidation cells, depolarization induces oxidative stress leads mitochondria-mediated autophagy, or mitophagy, results clearance damaged organelles under conditions, while this function also linked when dysregulated. role autophagy regulated ATG proteins PI3K/AKT/mTOR pathway dual: suppress sensitize cells therapy. A promising approach enhancing outcomes involves targeting mechanisms CICD, including inducing SLC7A11 inhibition, modulating ROS generation, combining inhibition with chemotherapy. Here, we review molecular underpinnings particularly on their transform treatment.

Язык: Английский

Процитировано

0
A novel prognostic signature related to programmed cell death in osteosarcoma DOI Creative Commons
Yuchen Jiang, Qitong Xu, Hongbin Wang

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Июль 1, 2024

Background Osteosarcoma primarily affects children and adolescents, with current clinical treatments often resulting in poor prognosis. There has been growing evidence linking programmed cell death (PCD) to the occurrence progression of tumors. This study aims enhance accuracy OS prognosis assessment by identifying PCD-related prognostic risk genes, constructing a PCD-based model, characterizing function genes within this model. Method We retrieved osteosarcoma patient samples from TARGET GEO databases, manually curated literature summarize 15 forms death. collated 1621 PCD sources as well databases such KEGG GSEA. To construct our we integrated ten machine learning methods including Enet, Ridge, RSF, CoxBoost, plsRcox, survivalSVM, Lasso, SuperPC, StepCox, GBM. The optimal model was chosen based on average C-index, named Programmed Cell Death Score (OS-PCDS). validate predictive performance across different datasets, employed three independent validation sets. Moreover, assessed mRNA protein expression levels included investigated their impact proliferation, migration, apoptosis cells gene knockdown experiments. Result In extensive analysis, identified 30 associated (OS). assess power these computed C-index for various combinations. that random survival forest (RSF) algorithm demonstrated superior performance, significantly outperforming traditional approaches. five key genes: MTM1, MLH1, CLTCL1, EDIL3, SQLE. relevance analyzed levels, revealing significant disparities between normal tissue cells. Specifically, were markedly altered cells, suggesting critical role tumor progression. Further functional performed through experiments U2OS Knockdown genes—CLTCL1, SQLE—resulted substantial changes proliferation rate, migration capacity, rate These findings underscore pivotal roles pathophysiology highlight potential therapeutic targets. Conclusion constituting OS-PCDS model—CLTCL1, SQLE—were found highlighting markers enables accurate evaluation patients osteosarcoma.

Язык: Английский

Процитировано

1
Leveraging single‐cell sequencing analysis and bulk‐RNA sequencing analysis to forecast necroptosis in cutaneous melanoma prognosis DOI
Jiaheng Xie, Pengpeng Zhang, Qikai Tang

и другие.

Experimental Dermatology, Год журнала: 2024, Номер 33(7)

Опубликована: Июль 1, 2024

Cutaneous melanoma, a malignancy of melanocytes, presents significant challenge due to its aggressive nature and rising global incidence. Despite advancements in treatment, the variability patient responses underscores need for further research into novel therapeutic targets, including role programmed cell death pathways such as necroptosis. The melanoma datasets used analysis, GSE215120, GSE19234, GSE22153 GSE65904, were downloaded from GEO database. data TCGA UCSC website. Using single-cell sequencing, we assess heterogeneity necroptosis cutaneous identifying distinct clusters necroptosis-related gene expression patterns. A combination 101 machine learning algorithms was employed construct signature (NRS) based on key genes associated with prognostic value NRS evaluated four cohorts (one three cohorts), tumour microenvironment (TME) analysed understand relationship between necroptosis, mutation burden (TMB) immune infiltration. Finally, focused target TSPAN10 prognosis, pathogenesis, immunotherapy relevance drug sensitivity melanoma. Our study revealed among cells, higher prevalence epithelial myeloid cells fibroblasts. NRS, developed through rigorous techniques, demonstrated robust capabilities, distinguishing high-risk patients poorer outcomes all cohorts. Analysis TME showed that high scores correlated lower TMB reduced infiltration, indicating potential mechanism which influences progression. has been identified is highly poor prognosis. findings highlight complex introduce tool guide decisions.

Язык: Английский

Процитировано

1
Programmed cell death pathways in lung adenocarcinoma: illuminating tumor drug resistance and therapeutic opportunities through single-cell analysis DOI Creative Commons
Long Li,

Shancheng He

Discover Oncology, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 23, 2024

Lung adenocarcinoma (LUAD) is a major contributor to cancer-related deaths, distinguished by its pronounced tumor heterogeneity and persistent challenges in overcoming drug resistance. In this study, we utilized single-cell RNA sequencing (scRNA-seq) dissect the roles of programmed cell death (PCD) pathways, including apoptosis, necroptosis, pyroptosis, ferroptosis, shaping LUAD heterogeneity, immune infiltration, prognosis. Among these, ferroptosis pyroptosis were most significantly associated with favorable survival outcomes, highlighting their potential enhancing anti-tumor immunity. Distinct PCD-related subtypes identified, characterized differential pathway activation composition. Subtypes enriched cytotoxic lymphocytes dendritic cells demonstrated improved outcomes increased responsiveness immunotherapy. Drug sensitivity analysis revealed that these exhibited heightened targeted therapies checkpoint inhibitors, suggesting opportunities for personalized treatment strategies. Our findings emphasize interplay between PCD pathways microenvironment, providing insights into mechanisms underlying resistance evasion. By linking molecular features clinical study highlights targeting enhance therapeutic efficacy overcome LUAD. These results contribute growing framework developing precise adaptable cancer tailored specific characteristics.

Язык: Английский

Процитировано

0
Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide DOI Open Access

Daria M. Yurkina,

Elena A. Romanova,

K.A. Shcherbakov

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(12), С. 6633 - 6633

Опубликована: Июнь 16, 2024

Receptors of cytokines are major regulators the immune response. In this work, we have discovered two new ligands that can activate TNFR1 (tumor necrosis factor receptor 1) receptor. Earlier, found peptide Tag (PGLYRP1) protein designated 17.1 interact with Here, Mts1 (S100A4) interacts a high affinity (Kd = 1.28 × 10-8 M), and complex is cytotoxic to cancer cells on their surface. This induces both apoptosis necroptosis in involvement mitochondria lysosomes cell death signal transduction. Moreover, succeeded locating fragment responsible for protein-peptide interaction, which highly specifically Tag7 2.96 nM). The isolated M7 also forms 17.1, peptide-peptide receptor-dependent death. Molecular docking molecular dynamics experiments show amino acids involved binding may be used peptidomimetics' development. Thus, complexes were created able induce tumor via These results therapy autoimmune diseases.

Язык: Английский

Процитировано

0