Manipulating the cGAS-STING Axis: advancing innovative strategies for osteosarcoma therapeutics
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 7, 2025
This
paper
explored
the
novel
approach
of
targeting
cyclic
guanosine
monophosphate
(GMP)-adenosine
(AMP)
synthase-stimulator
interferon
genes
(cGAS-STING)
pathway
for
treatment
osteosarcoma
(OS).
Osteosarcoma
is
a
common
malignancy
in
adolescents.
Most
patients
die
from
lung
metastasis.
It
reviewed
epidemiology
and
pathological
characteristics
OS,
highlighting
its
highly
malignant
nature
tendency
pulmonary
metastasis,
underscoring
importance
identifying
new
therapeutic
targets.
The
cGAS-STING
was
closely
associated
with
biological
behaviors
OS
cells,
suggesting
that
this
could
be
promising
strategy.
Currently,
research
on
role
has
been
limited,
underlying
mechanisms
remain
unclear.
Therefore,
further
investigation
into
exploration
strategies
based
are
great
significance
developing
more
effective
treatments
OS.
offered
fresh
perspective
providing
hope
options
by
pathway.
Язык: Английский
DNA methylation as an epigenetic mechanism in the regulation of LEDGF expression and biological response in aging and oxidative stress
Cell Death Discovery,
Год журнала:
2024,
Номер
10(1)
Опубликована: Июнь 22, 2024
The
physiological
quantum
of
stress-inducible
transcriptional
protein,
Lens
Epithelium-Derived
Growth
Factor
(LEDGF),
is
vital
for
the
maintenance
cellular
physiology.
Erratic
epigenetic
reprogramming
in
response
to
oxidative
stress
or
with
advancing
age
found
be
a
major
cause
gene
silencing,
leading
pathobiologies.
Using
aging
human
(h)
eye
lens/lens
epithelial
cells
(LECs)
coupled
redox-active
Peroxiredoxin
6
(Prdx6)-deficient
(Prdx6
Язык: Английский
New insights into the mechanisms of the immune microenvironment and immunotherapy in osteosarcoma
Frontiers in Immunology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 17, 2025
Osteosarcoma,
a
malignant
bone
tumor
primarily
affecting
adolescents,
is
highly
invasive
with
poor
prognosis.
While
surgery
and
chemotherapy
have
improved
survival
for
localized
cases,
pulmonary
metastasis
significantly
reduces
to
approximately
20%,
highlighting
the
need
novel
treatments.
Immunotherapy,
which
leverages
immune
system
target
osteosarcoma
cells,
shows
promise.
This
review
summarizes
biological
characteristics
of
osteosarcoma,
mechanisms
metastasis,
microenvironment
(TME).
It
involves
recent
immunotherapy
advances,
including
monoclonal
antibodies,
vaccines,
cell
therapies,
checkpoint
inhibitors,
oncolytic
viruses,
discusses
combining
these
standard
Язык: Английский
Cellular variability as a driver for bioprocess innovation and optimization
Biotechnology Advances,
Год журнала:
2025,
Номер
unknown, С. 108528 - 108528
Опубликована: Фев. 1, 2025
Cellular
heterogeneity
plays
a
crucial
role
in
biotechnological
processes,
significantly
influencing
metabolic
activity,
product
yield,
and
process
consistency.
This
review
explores
the
different
dimensions
of
cellular
heterogeneity,
focusing
on
its
manifestation
at
both
single-cell
population
levels.
The
study
examines
how
factors
such
as
asymmetric
cell
division,
age,
environmental
conditions
contribute
to
functional
diversity
within
populations,
with
an
emphasis
microorganisms
like
yeast.
Age-related
particular,
is
highlighted
for
impact
pathways,
mitochondrial
function,
secondary
metabolite
production,
which
directly
affect
bioprocess
outcomes.
Furthermore,
discusses
advanced
techniques
detecting
managing
including
surface
marker-based
approaches,
utilize
proteins,
polysaccharides,
lipids,
label-free
methods
that
leverage
volume
physical
properties
separation.
Understanding
controlling
essential
optimizing
industrial
bioprocesses,
improving
ensuring
quality.
also
underscores
potential
emerging
tools,
real-time
analysis
microfluidic
devices,
enhancing
separation
better
efficiency
robustness.
Язык: Английский
Identification and functional characterization of T-cell exhaustion-associated lncRNA AL031775.1 in osteosarcoma: a novel therapeutic target
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 24, 2025
Osteosarcoma,
an
aggressive
bone
malignancy
predominantly
affecting
children
and
adolescents,
presents
significant
therapeutic
challenges
with
a
5-year
survival
rate
below
30%
in
metastatic
cases.
T-cell
exhaustion,
characterized
by
the
overexpression
of
immune
checkpoint
molecules,
contributes
to
osteosarcoma
progression
evasion.
Although
targeting
these
inhibitory
pathways
has
shown
potential
restoring
activity,
molecular
regulators
depletion
are
poorly
understood.
This
study
employed
comprehensive
bioinformatics
analyses
on
samples
from
TARGET
database,
combined
normal
tissue
data
GTEx
identify
exhaustion-associated
genes
their
co-expressed
long
non-coding
RNAs
(lncRNAs).
Gene
ontology
KEGG
pathway
were
used
elucidate
immune-related
enrichments.
A
six-lncRNA
prognostic
model
was
established
using
LASSO
regression
validated
separate
cohorts.
Functional
assays
evaluated
impact
lncRNA
AL031775.1
cell
behavior
function.
Twenty-four
key
exhaustion-related
identified
significantly
enriched
pathways,
indicating
importance
microenvironment.
The
constructed
stratified
patients
prognosis,
showing
robust
predictive
performance
across
Among
six
lncRNAs,
is
notably
downregulated
promotes
proliferation,
migration,
invasion
while
contributing
exhaustion.
In
T
cells,
downregulation
impairs
antitumor
immunity,
upregulates
molecules
LAG3,
PD1,
CTLA4,
diminishes
cytotoxic
activity
against
tumor
cells.
identifies
novel
highlights
managing
enhancing
immunity
counteracting
progression.
Targeting
represents
promising
approach
improve
immunotherapy
efficacy
osteosarcoma.
These
findings
provide
critical
insights
into
regulation
exhaustion
suggest
new
avenue
for
intervention.
Язык: Английский
Identification of telomere-related gene subtypes and prognostic signatures in osteosarcoma
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 25, 2025
Osteosarcoma
(OS)
is
the
prevalent
primary
bone
cancer,
with
a
high
proclivity
for
local
invasion
and
metastasis.
Previous
studies
have
indicated
that
telomeres
are
closely
related
to
prognosis
of
but
significance
telomere-related
features
in
OS
remains
uncertain.
Thus,
goal
this
work
identified
subtypes
based
on
genes
(TRGs).
The
data
was
collected
from
TARGET
Gene
Expression
Omnibus
databases.
Firstly,
we
mediated
by
TRGs
OS.
Subsequently,
analyzed
immune
characteristics
telomeres-related
Moreover,
built
signature
via
univariate
LASSO
Cox
regression
analyses,
correlation
TME.
Finally,
expression
hub
We
discovered
could
distinguish
patients
into
two
(C1
C2).
survival
rate
C2
inferior
C1.
scores
stromal,
ESTIMATES
were
observably
increased,
tumor
purity
decreased
C1
compared
subtypes.
Differentially
expressed
between
highly
enriched
immune-related
pathways.
had
different
characteristic.
Furthermore,
telomere
prognostic
model
including
six
(PDK2,
PPARG,
MORC4,
SP110,
TERT
MAP3K5)
established
predict
patients.
High-risk
group
correlated
patients,
risk
score
PDK2,
MAP3K5
significantly
cells.
In
conclusion,
our
study
has
uncovered
importance
defining
distinct
outcomes
contexts.
developed
may
serve
as
valuable
tool
prediction
inform
future
therapeutic
strategies
targeting
TME
Язык: Английский
Emerging immunotherapies in osteosarcoma: from checkpoint blockade to cellular therapies
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Март 18, 2025
Osteosarcoma
remains
a
highly
aggressive
bone
malignancy
with
limited
therapeutic
options,
necessitating
novel
treatment
strategies.
Immunotherapy
has
emerged
as
promising
approach,
yet
its
efficacy
in
osteosarcoma
is
hindered
by
an
immunosuppressive
tumor
microenvironment
and
resistance
mechanisms.
This
review
explores
recent
advancements
checkpoint
blockade,
cellular
therapies,
combination
strategies
aimed
at
enhancing
immune
responses.
We
highlight
key
challenges,
including
heterogeneity,
poor
infiltration,
the
need
for
predictive
biomarkers.
By
integrating
immunotherapy
chemotherapy,
radiotherapy,
targeted
therapy,
emerging
approaches
seek
to
improve
outcomes.
provides
comprehensive
analysis
of
evolving
landscape
immunotherapy,
offering
insights
into
future
directions
potential
breakthroughs.
Researchers
clinicians
will
benefit
from
understanding
these
developments,
they
pave
way
more
effective
personalized
osteosarcoma.
Язык: Английский
Identifying PSIP1 as a critical R-loop regulator in osteosarcoma via machine-learning and multi-omics analysis
Cancer Cell International,
Год журнала:
2025,
Номер
25(1)
Опубликована: Апрель 22, 2025
Dysregulation
of
R-loops
has
been
implicated
in
tumor
development,
progression,
and
the
regulation
immune
microenvironment
(TME).
However,
their
roles
osteosarcoma
(OS)
remain
underexplored.
In
this
study,
we
firstly
constructed
a
novel
R-loop
Gene
Prognostic
Score
Model
(RGPSM)
based
on
RNA-sequencing
(RNA-seq)
datasets
evaluated
relationships
between
RGPSM
scores
TME.
Additionally,
identified
key
R-loop-related
genes
involved
OS
progression
using
single-cell
RNA
sequencing
(scRNA-seq)
dataset,
validated
these
findings
through
experiments.
We
found
that
patients
with
high-RGPSM
exhibited
poorer
prognosis,
lower
Huvos
grades
more
suppressive
Moreover,
proportion
malignant
cells
was
significantly
higher
group.
And
integrated
analysis
RNA-seq
scRNA-seq
revealed
PC4
SRSF1
Interacting
Protein
1
(PSIP1)
highly
expressed
osteoblastic
proliferative
cells.
Notably,
high
expression
PSIP1
associated
poor
prognosis
patients.
Subsequent
experiments
demonstrated
knockdown
inhibited
both
vivo
vitro,
leading
increased
accumulation
DNA
damage.
Conversely,
overexpression
facilitated
resolution
reduced
damage
induced
by
cisplatin.
conclusion,
developed
effectively
predicted
outcomes
across
diverse
cohorts
as
critical
modulator
regulating
Язык: Английский
Multi-omics and Machine Learning-driven CD8+ T Cell Heterogeneity Score for Prognosis and Personalized Therapy Outcomes Prediction in Head and Neck Squamous Cell Carcinoma
Molecular Therapy — Nucleic Acids,
Год журнала:
2024,
Номер
36(1), С. 102413 - 102413
Опубликована: Дек. 5, 2024
Язык: Английский