The role of splicing events in the inflammatory response of atherosclerosis: molecular mechanisms and modulation DOI Creative Commons

Aolong Wang,

Chengzhi Wang,

Bihan Xuan

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Дек. 17, 2024

Atherosclerosis is a chronic inflammatory disease characterized by persistent responses throughout all stages of its progression. Modulating these promising avenue for the development cardiovascular therapies. Splicing events modulate gene expression and diversify protein functionality, exerting pivotal roles in mechanisms underlying atherosclerosis. These insights may provide novel opportunities developing anti-inflammatory therapies this disease. This article systematically discusses diverse splice variants how splicing impact response atherosclerosis via endothelial cells, macrophages, vascular smooth muscle highlighting their molecular implications. Furthermore, study summarizes clinical evidence supporting splicing-related molecules as diagnostic biomarkers therapeutic targets Lastly, we outline current challenges future research directions concerning offers perspective formulating new strategies aimed at lowering risk

Язык: Английский

Molecular mimicry as a mechanism of viral immune evasion and autoimmunity DOI Creative Commons
Cole Maguire,

Chumeng Wang,

Akshara Ramasamy

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Окт. 30, 2024

Язык: Английский

Процитировано

6

Unlocking the secrets of the immunopeptidome: MHC molecules, ncRNA peptides, and vesicles in immune response DOI Creative Commons
Arpita Balakrishnan,

Gabriela Winiarek,

Olga Hołówka

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Янв. 29, 2025

The immunopeptidome, a diverse set of peptides presented by Major Histocompatibility Complex (MHC) molecules, is critical component immune recognition and response. This review article delves into the mechanisms peptide presentation MHC particularly emphasizing roles ncRNA-derived extracellular vesicles (EVs) in shaping immunopeptidome landscape. We explore established emerging insights molecule interactions with peptides, including dynamics loading, transport, influence cellular genetic variations. highlights novel research on non-coding RNA (ncRNA)-derived which challenge conventional views antigen processing role EVs transporting these thereby modulating responses at remote body sites. not only challenges but also opens up new avenues for understanding responses. Furthermore, we discuss implications developing therapeutic strategies, cancer immunotherapy. By conducting comprehensive analysis current literature advanced methodologies immunopeptidomics, this aims to deepen complex interplay between system, offering perspectives potential diagnostic applications. Additionally, provide mechanism enhanced surface highlight pathway their systemic distribution, potentially altering surveillance landscapes.

Язык: Английский

Процитировано

0

Harnessing nanoparticles for reshaping tumor immune microenvironment of hepatocellular carcinoma DOI Creative Commons
Jinsong Li,

GuanBo Zhang,

Gang Li

и другие.

Discover Oncology, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 5, 2025

Hepatocellular carcinoma (HCC) is one of the most prevalent cancers, characterized by high morbidity and mortality rates. Recently, immunotherapy has emerged as a crucial treatment modality for HCC, following surgery, locoregional therapies, targeted therapies. This approach harnesses body's immune system to target eliminate cancer cells, potentially resulting in durable antitumor responses. However, acquired resistance tumor immunosuppressive microenvironment (TIME) significantly hinder its clinical application. advancements nanotechnology, coupled with deeper understanding biology nano-biological interactions, have led development various nanoparticles aimed at enhancing therapeutic efficacy through specific targeting tissues. These increase accumulation immunotherapeutic drugs within microenvironment, thereby transforming TIME. In this review, we provide concise overview fundamental principles governing TIME landscape HCC discuss rationale applications context. Additionally, highlight existing challenges potential opportunities translation nanomedicines.

Язык: Английский

Процитировано

0

Alternative Splicing as a Modulator of the Interferon-Gamma Pathway DOI Open Access
Parul Suri,

Ariana Badalov,

Matteo Ruggiu

и другие.

Cancers, Год журнала: 2025, Номер 17(4), С. 594 - 594

Опубликована: Фев. 10, 2025

Interferon-gamma (IFN-γ) is a critical cytokine that plays pivotal role in immune system regulation. It key mediator of both cellular defense mechanisms and antitumor immunity. As the sole member type II interferon family, IFN-γ modulates responses by activating macrophages, enhancing natural killer cell function, regulating gene expression across multiple processes. Alternative splicing post-transcriptional regulatory mechanism generates mature messenger RNAs from single gene, dramatically increasing proteome diversity without need proportional genome expansion. This process occurs 90–95% human genes, with alternative events allowing for production diverse protein isoforms can have distinct—or even opposing—functional properties. crucial cancer immunology, potentially generating tumor neoepitopes modulating responses. However, how affects IFN-γ’s activity still poorly understood. review explores regulates function upstream regulators downstream effectors IFN-γ, revealing complex response modulation. Key transcription factors signaling molecules pathway are alternatively spliced, alter signaling, to environmental cues. Specific splice variants enhance or inhibit IFN-γ-mediated responses, influencing immunotherapy, autoimmune conditions, infectious disease outcomes. The emerging understanding these offers promising therapeutic strategies manipulating through targeted molecular interventions.

Язык: Английский

Процитировано

0

Advancements and challenges in personalized neoantigen-based cancer vaccines DOI Creative Commons

Parminder Singh,

Mahalaqua Nazli Khatib,

R Roopashree

и другие.

Oncology Reviews, Год журнала: 2025, Номер 19

Опубликована: Март 14, 2025

Advancements in personalized neoantigen-based cancer vaccines are ushering a new era oncology, targeting unique genetic alterations within tumors to enhance treatment precision and efficacy. Neoantigens, specific cells absent normal tissues, at the heart of these vaccines, promising direct immune system specifically against tumor, thereby maximizing therapeutic efficacy while minimizing side effects. The identification neoantigens through genomic proteomic technologies is central developing allowing for precise mapping tumor's mutational landscape. Despite advancements, accurately predicting which will elicit strong responses remains challenging due tumor variability complexity interactions. This necessitates further refinement bioinformatics tools predictive models. Moreover, heavily depends on innovative delivery methods that neoantigen presentation system. Techniques like encapsulating lipid nanoparticles using viral vectors critical improving vaccine stability delivery. Additionally, contribute towards achieving Sustainable Development Goal 3.8, promoting universal health coverage by advancing access safe effective treatments. review delves into potential transform treatment, examining both revolutionary advancements ongoing challenges they face.

Язык: Английский

Процитировано

0

Gingipain regulates isoform switches of PD-L1 in macrophages infected with Porphyromonas gingivalis DOI Creative Commons

Yilin Zheng,

Ziyi Wang, Yao Weng

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 26, 2025

Periodontal pathogen Porphyromonas gingivalis (P. gingivalis) is believed to possess immune evasion capabilities, but it remains unclear whether this related host gene alternative splicing (AS). In study, RNA-sequencing revealed significant changes in both AS landscape and transcriptomic profile of macrophages following P. infection with/without knockout gingipain (a unique toxic protease gingivalis). increased the PD-L1 transcripts expression selectively upregulated a specific coding isoform that more effectively binds PD-1 on T cells, thereby inhibiting function. Biological experiments also detected switch gingivalis-infected or gingipain-treated macrophages. AlphaFold 3 predictions indicated protein docking compatibility between gingivalis-upregulated was over 80% higher than another isoform. These findings suggest employs modulate PD-L1, facilitating evasion.

Язык: Английский

Процитировано

0

Harnessing antibody-mediated recognition of the intracellular proteome with T cell receptor-like specificity DOI Creative Commons

Maya Haus‐Cohen,

Yoram Reiter

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 22, 2024

The clinical success of cancer immunotherapy has driven ongoing efforts to identify novel targets that can effectively guide potent effector functions eliminate malignant cells. Traditionally, immunotherapies have focused on surface antigens; however, these represent only a small fraction the proteome, limiting their therapeutic potential. In contrast, majority proteins within human proteome are intracellular, yet they represented cell as short peptides presented by MHC class I molecules. These peptide-MHC complexes offer vast and largely untapped resource for targets. intracellular including neo-antigens, presents an exciting opportunity development cell-based soluble immunotherapies. Targeting intracellular-derived molecules surfaces be achieved using specific T-cell receptors (TCRs) or TCR-mimicking antibodies, known TCR-like (TCRL) antibodies. Current strategies under investigation include adoptive transfer TCR-engineered TCRL-T cells CAR-T target complexes, well TCR- TCRL-based agents like bispecific T engagers. Recent developments in targeting TCRL- TCR-based shown promising results, with two therapies recently receiving FDA approval treatment unresectable metastatic uveal melanoma synovial sarcoma. This review focuses processes selecting isolating moieties, emphasis pre-clinical studies explore potential immunotherapy.

Язык: Английский

Процитировано

0

Gingipain regulates isoform switches of PD-L1 in macrophages infected with Porphyromonas gingivalis DOI Creative Commons

Yilin Zheng,

Ziyi Wang, Yao Weng

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 29, 2024

Abstract Periodontal pathogen Porphyromonas gingivalis(Pg) is believed to possess immune evasion capabilities, but it remains unclear whether this related host gene alternative splicing (AS). In study, RNA-sequencing (RNA-seq) revealed significant changes in both AS landscape and transcriptomic profile of macrophages following Pg infection with/without knockout gingipain (a unique toxic protease Pg). increased the programmed death ligand 1 (PD-L1) transcripts expression selectively upregulated a specific coding isoform that more effectively binds cell protein (PD-1) receptors on T cells, thereby inhibiting function. Biological experiments confirmed these results demonstrated switch PD-L1 was gingipain-dependent. AlphaFold 3 predictions indicated docking compatibility between PD-1 Pg-upregulated over 80% higher than another isoform. These findings suggest Pg employs modulate PD-L1, facilitating evasion.

Язык: Английский

Процитировано

0

The role of splicing events in the inflammatory response of atherosclerosis: molecular mechanisms and modulation DOI Creative Commons

Aolong Wang,

Chengzhi Wang,

Bihan Xuan

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Дек. 17, 2024

Atherosclerosis is a chronic inflammatory disease characterized by persistent responses throughout all stages of its progression. Modulating these promising avenue for the development cardiovascular therapies. Splicing events modulate gene expression and diversify protein functionality, exerting pivotal roles in mechanisms underlying atherosclerosis. These insights may provide novel opportunities developing anti-inflammatory therapies this disease. This article systematically discusses diverse splice variants how splicing impact response atherosclerosis via endothelial cells, macrophages, vascular smooth muscle highlighting their molecular implications. Furthermore, study summarizes clinical evidence supporting splicing-related molecules as diagnostic biomarkers therapeutic targets Lastly, we outline current challenges future research directions concerning offers perspective formulating new strategies aimed at lowering risk

Язык: Английский

Процитировано

0