Host species and habitat shape fish-associated bacterial communities: phylosymbiosis between fish and their microbiome

Microbiome, Год журнала: 2023, Номер 11(1)

Опубликована: Ноя. 20, 2023

While many studies have reported that the structure of gut and skin microbiota is driven by both species-specific habitat-specific factors, relative importance host-specific versus environmental factors in wild vertebrates remains poorly understood. The aim this study was to determine diversity composition fish skin, gut, surrounding water bacterial communities (hereafter referred as microbiota) assess extent which host habitat phylogeny predict similarity. Skin swabs samples from 334 belonging 17 species were sampled three Laurentian Great Lakes (LGLs) habitats (Detroit River, Lake Erie, Ontario). We also collected filtered at time collection. analyzed community using 16S metabarcoding tested for variation.We found distinct microbiota, although more closely resembled microbiota. (sample location), habitat, diet, shape promote divergence or convergence Since significantly affected (separately effects), we phylosymbiosis pairwise phylogenetic distance dissimilarity. significant effects on dissimilarity, consistent with perhaps reflecting longstanding co-evolutionary relationship between their microbiomes.Analyzing mucus across diverse complex natural ecosystems such LGLs provides insights into potential microbiome, ultimately health, host. Video Abstract.

Язык: Английский

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The intestinal digesta microbiota of tropical marine fish is largely uncultured and distinct from surrounding water microbiota

npj Biofilms and Microbiomes, Год журнала: 2024, Номер 10(1)

Опубликована: Фев. 19, 2024

Studying the gut microbes of marine fishes is an important part conservation as many fish species are increasingly threatened by extinction. The microbiota only a small fraction more than 32,000 known has been investigated. In this study we analysed intestinal digesta composition 50 different wild from tropical waters. Our results show that harbour distinct surrounding water and location, domestication status, host intrinsic factors strongly associated with composition. Furthermore, vast majority (~97%) fish-associated microorganisms do not have any cultured representative. Considering impact on health physiology, these findings underpin call to also preserve species, especially those may be exposed habitat destruction.

Язык: Английский

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Gut Immune System and the Implications of Oral-Administered Immunoprophylaxis in Finfish Aquaculture

Frontiers in Immunology, Год журнала: 2021, Номер 12

Опубликована: Дек. 16, 2021

The gastrointestinal immune system plays an important role in homeostasis regulation. It regulates the symbiotic host-microbiome interactions by training and developing host’s innate adaptive immunity. This interaction a vital host defence mechanisms at same time, balancing endogenous perturbations of homeostasis. fish is armed with intricate diffused gut-associated lymphoid tissues (GALTs) that establish tolerance toward enormous commensal gut microbiome while preserving responses against intrusion enteric pathogens. A comprehensive understanding intestinal prerequisite for oral vaccine immunostimulants aquaculture, particularly cultured species. In this review, we outline remarkable features immunity essential components tissue. mechanistic principles underlying antigen absorption uptake through epithelial, subsequent activation series molecular events are reviewed. emphasis on significance administration immunoprophylactics, different potential adjuvants circumvent tolerance. Comprehension pivotal effective vaccines can be delivered orally, which less labour-intensive could improve health facilitate disease management aquaculture industry.

Язык: Английский

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Gut microbiomes of cyprinid fish exhibit host-species symbiosis along gut trait and diet

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Авг. 9, 2022

Teleost omnivorous fish that coexist partially sharing resources are likely to modify their gut traits and microbiome as a feedback mechanism between ecological processes evolution. However, we do not understand how the core supports metabolic capacity of host regulates digestive functions in specialized traits. Therefore, evaluated eight from single family (i.e., Cyprinidae) current study. We examined correlation phylogeny, diet composition, intestinal morphological related microbiome. The results indicated cyprinid with similar relative lengths had considerable similarity. Notably, SL (short length) group, zoobenthos zooplankton specialists, was abundant Proteobacteria less Firmicutes than ML (medium LL (long groups. These could extract nutrients aquatic plants algae. Additionally, found abundance Clostridium Romboutsia be positively correlated length but negatively Cetobacterium, Plesiomonas, Bacteroides, Lactobacillus, host-relative length. also show positive linear relationship carbohydrate metabolism length, while amino acid lipid In addition, species competing for improve adaptability through specialization which is closely variation synergy Above all, microbiota associated morphologies enable variably tolerate resource fluctuation utilization efficiency nutrient extraction challenging food resources.

Язык: Английский

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Regulation of host gene expression by gastrointestinal tract microbiota in Chinook Salmon (Oncorhynchus tshawytscha)

Molecular Ecology, Год журнала: 2023, Номер 32(15), С. 4427 - 4446

Опубликована: Июнь 7, 2023

Abstract Differences in gut microbiome composition are linked with health, disease and ultimately host fitness; however, the molecular mechanisms underlying that relationship not well characterized. Here, we modified fish microbiota using antibiotic probiotic feed treatments to address effect of on gene expression patterns. Chinook salmon ( Oncorhynchus tshawytscha ) was evaluated whole transcriptome sequencing (RNA‐Seq) hindgut mucosa samples from individuals treated antibiotic, control diets determine differentially expressed (DE) genes. Fifty DE genes were selected for further characterization nanofluidic qPCR chips. We used 16S rRNA metabarcoding characterize rearing water (bacterial) communities. Daily administration antibiotics probiotics resulted significant changes aquatic as more than 100 treatment fish, relative healthy controls. Normal depletion by mostly led downregulation different aspects immunity upregulation apoptotic process. In treatment, related post‐translation modification inflammatory responses up‐regulated Our results revealed effects (antibiotic probiotic) rabep2, aifm3, manf, prmt3 transcription. Moreover, found associations between members Lactobacillaceae Bifidobacteriaceae Overall, our analysis showed had impacts many signalling pathways, specifically targeting immune, developmental metabolic processes. some involved microbiome‐host interactions will help develop new strategies preventing/ treating disruption‐related diseases.

Язык: Английский

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Diversity and structure of sparids external microbiota (Teleostei) and its link with monogenean ectoparasites

Animal Microbiome, Год журнала: 2022, Номер 4(1)

Опубликована: Апрель 13, 2022

Animal-associated microbial communities appear to be key factors in host physiology, ecology, evolution and its interactions with the surrounding environment. Teleost fish have received relatively little attention study of surface-associated microbiota. Besides important role microbiota homeostasis infection prevention, a few recent studies shown that mucus may interact attract some specific parasitic species. However, our understanding external assemblages, particular regarding determine their composition potential parasites, is still limited. This objective present focuses on well-known fish-parasite interaction, involving Sparidae (Teleostei), monogenean ectoparasites Lamellodiscus genus. We characterized skin gill bacterial using 16S rRNA amplicon sequencing, tested how ecological traits evolutionary history are related microbiota, assessed if taxa species.Our results revealed significant differences between terms diversity structure, sparids establish maintain tissue species-specific despite continuous exposure water. No phylosymbiosis pattern was detected for either or suggesting other host-related environmental better regulator host-microbiota interactions. Diversity structure were explained by traits: species, diet body part. Numerous correlations abundance given genera species been found mucus, including associations. also only unparasitized sparid this study, Boops boops, harbored significantly more Fusobacteria three genera, Shewenella, Cetobacterium Vibrio, compared involvement preventing infection.This first explore from wild family novel evidence links abundance, paving way further host-microbiota-parasite

Язык: Английский

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Gut microbiome composition associates with corticosteroid treatment, morbidity, and senescence in Chinook salmon (Oncorhynchus tshawytscha)

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Фев. 13, 2023

Abstract Pacific salmon experience prolonged elevation in corticosteroid hormones during important life history events including migration, reproduction, and senescence. These periods of elevated corticosteroids correspond with changes to immunity energy metabolism; therefore, fish may be particularly vulnerable mortality at these times. Recent studies found that stress-induced cortisol release associated microbial community shifts salmonids, raising the question how longer-term dynamics accompany transitions affect salmonid microbiomes. In this work, we experimentally evaluated relationships between gut microbiome composition, chronically corticosteroids, juvenile Chinook ( Oncorhynchus tshawytscha ). We treatment slow-release implants or dexamethasone resulted microbiome. Morbidity was also suggesting reflects individual differences susceptibility opportunistic pathogens. Additionally, analyzed a small number samples from adult various stages Results suggest composition integrity, communities treated juveniles shift toward those senescent adults. Overall, findings work indicate correlates risk chronic elevation.

Язык: Английский

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An integrative perspective on fish health: Environmental and anthropogenic pathways affecting fish stress

Marine Pollution Bulletin, Год журнала: 2023, Номер 194, С. 115318 - 115318

Опубликована: Авг. 3, 2023

Язык: Английский

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The Effects of Porphyra yezoensis Polysaccharides on Intestinal Health of Spotted Sea Bass, Lateolabrax maculatus

Fishes, Год журнала: 2023, Номер 8(8), С. 419 - 419

Опубликована: Авг. 15, 2023

Porphyra yezoensis polysaccharides (PPs) have biological activities such as promoting digestion, functioning antioxidants, and improving intestinal health. The aim of this study was to investigate the effects PPs on health spotted sea bass (Lateolabrax maculatus). A total 360 (10.53 ± 0.02 g) were randomly divided into six groups. Fish in each group fed with varying PP concentrations (0, 3, 6, 9, 12, 15 g/kg) for 52 days. results showed that 12 g/kg significantly increased body weight gain, specific growth rate, condition factor, while there no significant change feed conversion ratio. supplementation dosage 9 amylase activity, trypsin activity. In addition, compared control group, difference lipase experimental When g/kg, glutathione content increased, malondialdehyde decreased; effect catalase activity not significant. improved morphology, specifically by villus morphology increasing lining surface area. Compared abundance Firmicutes Bacteroides. At genus level, Cyanobacteria, Muribaculaceae, Lachnospiraceae main flora tract bass. ACE Chao1 indices microorganisms, Simpson index Shannon did significantly, indicating microbial composition had a certain extent. indicated different levels can improve physiological biochemical indexes, composition, thus

Язык: Английский

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Host diet drives gut microbiome convergence between coral reef fishes and mammals

Molecular Ecology, Год журнала: 2024, Номер 33(19)

Опубликована: Авг. 28, 2024

Abstract Animal gut microbiomes are critical to host physiology and fitness. The of fishes—the most abundant diverse vertebrate clade—have received little attention relative other clades. Coral reef fishes, in particular, make up a wide range evolutionary histories feeding ecologies that likely associated with microbiome diversity. repeated evolution herbivory fishes mammals also allows us examine similarity relationship diet across the entire tree life. Here, we generate large coral fish dataset ( n = 499 samples, 19 species) combine it aggregation public data 447) show drives significant convergence between mammalian microbiomes. We demonstrate this is largely driven by carnivory herbivorous carnivorous hosts exhibit distinct microbial compositions mammals. mammal share prominent taxa, including Ruminoccocus spp. Akkermansia spp., predicted metabolic pathways. Despite major ecological differences mammals, our results reveal their undergo similar dietary selective pressures. Thus, diet, addition phylosymbiosis must be considered even when comparing distantly related hosts.

Язык: Английский

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