Assessment of Coastal Biodiversity: Eukaryotic Communuties Through DNA Metabarcoding Along Artificial Coastlines DOI
Aydın Kaleli, E. Gözde Özbayram, Reyhan Akçaalan

и другие.

Опубликована: Янв. 1, 2024

In natural coastal habitats, biofilms can support the growth of substrate-specific organisms. Organisms in flourish these areas and provide valuable ecological data for biomonitoring. this study, we aimed to assess eukaryotic community on substrates along coasts Istanbul. We used 18S rRNA gene-targeted metabarcoding reveal species diversity. The was composed a total 361 genera belonging 5 supergroups. Archaeplastida most abundant supergroup, it followed by Amorphea Arthropoda. PERMANOVA analysis revealed significant seasonal differences heterogeneous observed. Salinity, phosphorus, dissolved oxygen were main parameters causing variance community. Additionally, several non-indigenous species, such as Mytilus, high number observations temporal succession showed that is promising tool artificial hard substrates.

Язык: Английский

Metabarcoding identifies macroalgal composition as a driver of benthic invertebrate assemblages in restored habitats DOI Creative Commons
Cristina Galobart, Jesús Zarcero, Adrià Antich

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 21, 2025

Abstract Large brown macroalgal forests support diverse communities of associated invertebrates. However, human activities have led to their degradation, replacing the original assemblages with less-complex habitats and altering invertebrate communities. To counteract this, restoration actions are currently being performed aiming at recovery. Here, we used metabarcoding COI gene describe community a 10-year restored forest dominated by Gongolaria barbata . This assemblage was compared an adjacent site where G. is absent, two healthy also , as references. Results showed that arthropods, molluscs, annelids were most abundant groups. Specific MOTUs representing sponge ascidian species exclusively found in assemblages, suggesting particularly benefited some these organisms. Crustaceans however, similarly across including one photophilous macroalgae (without ), thus indicating mobile groups may equally colonise alternative habitats. The overall composition did not completely resemble references, recovering all diversity complex process, likely influenced multiple factors require further investigation.

Язык: Английский

Процитировано

0
Why Study Biodiversity in the Neotropics? DOI
Patrícia Domingues de Freitas,

Pedro Manoel Galetti

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Molecular tools prove little auks from Svalbard are extremely selective for Calanus glacialis even when exposed to Atlantification DOI Creative Commons
Kaja Bałazy, Emilia Trudnowska, Katarzyna Wojczulanis‐Jakubas

и другие.

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Авг. 22, 2023

Two Calanus species, C. glacialis and finmarchicus, due to different life strategies environmental preferences act as an ecological indicators of Arctic Atlantification. Their high lipid content makes them important food source for higher trophic levels ecosystems including the most abundant Northern Hemisphere's seabird, little auk (Alle alle). Recent studies indicate a critical need use molecular methods reliably identify these two sympatric species. We performed genetic morphology-based identification 2600 individuals collected in auks foraging grounds diet summer seasons 2019-2021 regions Svalbard with varying Genetic proved that 40% were wrongly classified finmarchicus according both types samples. The consisted almost entirely even more Atlantified regions. Due substantial bias identification, we expect scale northern expansion boreal may have been largely overestimated costs birds exposed Atlantification could be mostly driven by decrease size rather than shift from finmarchicus.

Язык: Английский

Процитировано

8
The Tree of Life eDNA metabarcoding reveals a similar taxonomic richness but dissimilar evolutionary lineages between seaports and marine reserves DOI Creative Commons
Bastien Macé, David Mouillot, Alicia Dalongeville

и другие.

Molecular Ecology, Год журнала: 2024, Номер 33(12)

Опубликована: Май 4, 2024

Abstract Coastal areas host a major part of marine biodiversity but are seriously threatened by ever‐increasing human pressures. Transforming natural coastlines into urban seascapes through habitat artificialization may result in loss and key ecosystem functions. Yet, the extent to which seaports differ from nearby habitats reserves across whole Tree Life is still unknown. This study aimed assess level α β‐diversity between reserves, whether these patterns conserved taxa evolutionary lineages. For that, we used environmental DNA (eDNA) metabarcoding survey six on French Mediterranean coast four strictly no‐take nearby. By targeting different groups—prokaryotes, eukaryotes, metazoans fish—with appropriate markers, provide holistic view contrasted habitats. In absence comprehensive reference databases, bioinformatic pipelines gather similar sequences molecular operational taxonomic units (MOTUs). contrast our expectations, obtained no difference MOTU richness (α‐diversity) except for prokaryotes fishes with higher diversity than seaports. However, observed marked dissimilarity (β‐diversity) all taxa. Surprisingly, this signature was preserved Life, up order. reveals that share few lineages along urbanized coasts suggests differences terms functioning both

Язык: Английский

Процитировано

3
A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous species DOI Creative Commons
Jesús Zarcero, Adrià Antich, Marc Rius

и другие.

iScience, Год журнала: 2023, Номер 27(1), С. 108588 - 108588

Опубликована: Ноя. 25, 2023

Metabarcoding techniques are revolutionizing studies of marine biodiversity. They can be used for monitoring non-indigenous species (NIS) in ports and harbors. However, they often biased by inconsistent sampling methods incomplete reference databases. Logistic constraints prompt the development simple, easy-to-deploy samplers. We tested a new device called polyamide mesh organismal (POMPOM) with high surface-to-volume ratio. POMPOMS were deployed inside fishing recreational port Mediterranean alongside conventional settlement plates. also compiled curated database cytochrome oxidase (COI) sequences NIS. COI metabarcoding communities settled POMPOMs captured similar biodiversity than plates, shared molecular operational units (MOTUs) representing ca. 99% reads. 38 NIS detected accounting 26% easy to deploy handle provide an efficient method surveillance.

Язык: Английский

Процитировано

5
Assessment of Coastal Biodiversity: Eukaryotic Communuties Through DNA Metabarcoding Along Artificial Coastlines DOI
Aydın Kaleli, E. Gözde Özbayram, Reyhan Akçaalan

и другие.

Опубликована: Янв. 1, 2024

In natural coastal habitats, biofilms can support the growth of substrate-specific organisms. Organisms in flourish these areas and provide valuable ecological data for biomonitoring. this study, we aimed to assess eukaryotic community on substrates along coasts Istanbul. We used 18S rRNA gene-targeted metabarcoding reveal species diversity. The was composed a total 361 genera belonging 5 supergroups. Archaeplastida most abundant supergroup, it followed by Amorphea Arthropoda. PERMANOVA analysis revealed significant seasonal differences heterogeneous observed. Salinity, phosphorus, dissolved oxygen were main parameters causing variance community. Additionally, several non-indigenous species, such as Mytilus, high number observations temporal succession showed that is promising tool artificial hard substrates.

Язык: Английский

Процитировано

0