Quorum-Sensing Regulation of Antimicrobial Resistance in Bacteria

Microorganisms, Год журнала: 2020, Номер 8(3), С. 425 - 425

Опубликована: Март 17, 2020

Quorum sensing is a cell-to-cell communication system that exists widely in the microbiome and related to cell density. The high-density colony population can generate sufficient number of small molecule signals, activate variety downstream cellular processes including virulence drug resistance mechanisms, tolerate antibiotics, harm host. This article gives general introduction current research status microbial quorum-sensing systems, focuses on role systems regulating such as efflux pump biofilm formation regulation, discusses new strategy for treatment drug-resistant bacteria proposed by using quorum quenching prevent resistance.

Язык: Английский

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Genetic diversity, mobilisation and spread of the yersiniabactin-encoding mobile element ICEKp in Klebsiella pneumoniae populations

Microbial Genomics, Год журнала: 2018, Номер 4(9)

Опубликована: Июль 9, 2018

Mobile genetic elements (MGEs) that frequently transfer within and between bacterial species play a critical role in evolution, often carry key accessory genes associate with bacteria's ability to cause disease. MGEs carrying antimicrobial resistance (AMR) and/or virulence determinants are common the opportunistic pathogen Klebsiella pneumoniae, which is leading of highly drug-resistant infections hospitals. Well-characterised K. pneumoniae include polyketide synthesis loci ybt clb (also known as pks), encoding iron-scavenging siderophore yersiniabactin genotoxin colibactin, respectively. These located an MGE called ICEKp, most virulence-associated providing mechanism for these factors spread population. Here we apply population genomics investigate prevalence, evolution mobility populations through comparative analysis 2498 whole-genome sequences. The locus was detected 40 % genomes, particularly amongst those associated invasive infections. We identified 17 distinct lineages 3 lineages, each one 14 different structural variants ICEKp. Comparison wider family Enterobacteriaceae revealed occasional ICEKp acquisition by other members. present all 38.4 ybt+ genomes. Hundreds independent integration events were affecting hundreds phylogenetically including at least 19 globally-disseminated carbapenem-resistant clone CG258. A novel plasmid-encoded form also identified, representing new dispersal populations. data indicate circulate freely population, among multidrug-resistant strains, should be considered target genomic surveillance along AMR determinants.

Язык: Английский

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Human, animal and environmental contributors to antibiotic resistance in low-resource settings: integrating behavioural, epidemiological and One Health approaches

Proceedings of the Royal Society B Biological Sciences, Год журнала: 2018, Номер 285(1876), С. 20180332 - 20180332

Опубликована: Апрель 11, 2018

Antibiotic resistance (ABR) is recognized as a One Health challenge because of the rapid emergence and dissemination resistant bacteria genes among humans, animals environment on global scale. However, there paucity research assessing ABR contemporaneously in low-resource settings. This critical review seeks to identify extent low- middle-income countries (LMICs). Existing has highlighted hotspots for environmental contamination; food-animal production systems that are likely harbour reservoirs or promote transmission well high increasing human rates colonization with commensal such Escherichia coli . very few studies have integrated all three components spectrum understand dynamics prevalence community-acquired humans animals. Microbiological, epidemiological social science needed at community population levels across order fill large gaps knowledge

Язык: Английский

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Faecal virome transplantation decreases symptoms of type 2 diabetes and obesity in a murine model

Gut, Год журнала: 2020, Номер 69(12), С. 2122 - 2130

Опубликована: Март 12, 2020

Development of obesity and type 2 diabetes (T2D) are associated with gut microbiota (GM) changes. The viral community is predominated by bacteriophages (phages), which viruses that attack bacteria in a host-specific manner. antagonistic behaviour phages has the potential to alter GM. As proof-of-concept, we demonstrate efficacy faecal virome transplantation (FVT) from lean donors for shifting phenotype obese mice into closer resemblance mice.The FVT consisted viromes distinct profiles extracted caecal content different vendors were fed low-fat (LF) diet 14 weeks. Male C57BL/6NTac divided five groups: LF (as control), high-fat (HF) diet, HF+ampicillin (Amp), HF+Amp+FVT HF+FVT. At weeks 6 7 study, HF+FVT treated oral gavage. Amp groups 24 hours prior first treatment.Six after FVT, showed significant decrease weight gain compared HF group. Further, glucose tolerance was comparable between mice, while other all had impaired tolerance. These observations supported shifts GM composition, blood plasma metabolome expression levels genes T2D development.Transfer communities an led reduced normalised parameters relative mice. We hypothesise this effect mediated via FVT-induced

Язык: Английский

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Microbiota–host communications: Bacterial extracellular vesicles as a common language

PLoS Pathogens, Год журнала: 2021, Номер 17(5), С. e1009508 - e1009508

Опубликована: Май 13, 2021

Both gram-negative and gram-positive bacteria release extracellular vesicles (EVs) that contain components from their mother cells. Bacterial EVs are similar in size to mammalian-derived thought mediate bacteria–host communications by transporting diverse bioactive molecules including proteins, nucleic acids, lipids, metabolites. have been implicated bacteria–bacteria interactions, promoting health or causing various pathologies. Although the science of bacterial is less developed than eukaryotic EVs, number studies on continuously increasing. This review highlights current state knowledge rapidly evolving field EV science, focusing discovery, isolation, biogenesis, more specifically role microbiota–host communications. Knowledge these mechanisms may be translated into new therapeutics diagnostics based EVs.

Язык: Английский

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Organoids and organs-on-chips: Insights into human gut-microbe interactions

Cell Host & Microbe, Год журнала: 2021, Номер 29(6), С. 867 - 878

Опубликована: Июнь 1, 2021

Язык: Английский

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Benefits of Immobilized Bacteria in Bioremediation of Sites Contaminated with Toxic Organic Compounds

Microorganisms, Год журнала: 2025, Номер 13(1), С. 155 - 155

Опубликована: Янв. 14, 2025

Although bioremediation is considered the most environmentally friendly and sustainable technique for remediating contaminated soil water, it effective when combined with physicochemical methods, which allow preliminary removal of large quantities pollutants. This allows microorganisms to efficiently eliminate remaining contaminants. In addition requiring necessary genes degradation pathways specific substrates, as well tolerance adverse environmental conditions, may perform below expectations. One typical reason this high toxicity xenobiotics present in concentrations, stemming from vulnerability bacteria introduced a site. especially true planktonic bacteria, whereas within biofilms or microcolonies have significant advantages over their counterparts. A physical matrix essential formation, maintenance, survival bacterial biofilms. By providing such immobilization, formation can be facilitated accelerated. Therefore, immobilization offers comprehensive solution cleanup by harnessing specialized metabolic activities while ensuring retention efficacy at target sites. many cases, also need methods that are otherwise required initially reduce contaminant concentrations. Then, will possible use remediation higher concentrations xenobiotics, significantly reducing costs maintaining rapid rate processes. review explores benefits highlighting materials processes developing an optimal matrix. It focuses on following four key areas: (i) types organic pollutants impacting human health, (ii) strains used processes, (iii) (iv) cells various carriers targeted pollutant degradation.

Язык: Английский

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Facilitation promotes invasions in plant‐associated microbial communities

Ecology Letters, Год журнала: 2018, Номер 22(1), С. 149 - 158

Опубликована: Ноя. 20, 2018

Abstract While several studies have established a positive correlation between community diversity and invasion resistance, it is less clear how species interactions within resident communities shape this process. Here, we experimentally tested antagonistic facilitative pairwise model microbial predict by the plant–pathogenic bacterium Ralstonia solanacearum . We found that promoted suppressed invasions both in lab tomato plant rhizosphere. Crucially, reliably explained observed outcomes also multispecies communities, mechanistically, was linked to direct inhibition of invader (antibiosis), lesser degree resource competition members invader. Together, our findings suggest type strength can outcome more complex communities.

Язык: Английский

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Rhizoremediation of petroleum hydrocarbons: a model system for plant microbiome manipulation

Microbial Biotechnology, Год журнала: 2018, Номер 11(5), С. 819 - 832

Опубликована: Июль 31, 2018

Phytoremediation is a green and sustainable alternative to physico-chemical methods for contaminated soil remediation. One of the flavours phytoremediation rhizoremediation, where plant roots stimulate microbes degrade organic contaminants. This approach particularly interesting as it takes advantage naturally evolved interaction mechanisms between microorganisms often results in complete mineralization contaminants (i.e. transformation water CO2 ). However, many biotic abiotic factors influence outcome this interaction, resulting variable efficiency remediation process. The difficulty predict precisely timeframe associated with rhizoremediation leads low adoption rates technology. Here, we review recent literature related particular focus on organisms. We then expand potential be model plant-microbe system microbiome manipulation studies.

Язык: Английский

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Microbial biofilms in nature: unlocking their potential for agricultural applications

Journal of Applied Microbiology, Год журнала: 2020, Номер 129(2), С. 199 - 211

Опубликована: Фев. 8, 2020

Soil environments are dynamic and the plant rhizosphere harbours a phenomenal diversity of micro‐organisms which exchange signals beneficial nutrients. Bipartite or symbiotic interactions with host roots, such as mycorrhizae various bacteria, relatively well characterized. In addition, tripartite interaction also exists between arbuscular mycorrhizal fungi (AMF) associated bacteria. Bacterial biofilms exist sheet bacterial cells in association AMF structures, embedded within self‐produced exopolysaccharide matrix. Such may play important functional roles these interactions. However, details about their relevant relationships have not been elucidated. This review explores current understanding naturally occurring microbial biofilms, biotic surfaces, especially AMF. The possible played by potential for application more productive sustainable agriculture is discussed this review.

Язык: Английский

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