The Use of Wheat Starch as Gelling Agent for In Vitro Proliferation of Blackberry (Rubus fruticosus L.) Cultivars and the Evaluation of Genetic Fidelity after Repeated Subcultures DOI Creative Commons
Doina Clapa, Monica Hârța, Katalin Szabo

и другие.

Horticulturae, Год журнала: 2023, Номер 9(8), С. 902 - 902

Опубликована: Авг. 8, 2023

Micropropagation has an important role in the large-scale production of blackberry plant material, given high proliferation rates this species. The aim present study was to evaluate proliferative capacity grown vitro on wheat starch-gelled culture medium compared classical agar-gelled and assess genetic fidelity between proliferated shoots their mother plants. Six varieties (‘Čačanska Bestrna’, ‘Chester Thornless’, ‘Driscoll’s Victoria’, ‘Loch Ness’, ‘Polar’, ‘Karaka Black’) were tested. For proliferation, Murashige Skoog (MS) supplemented with 0.5 mg dm−3 6-benzyladenine (BA) used. conventional gelled 0.5% agar, starch used as alternative gelling agent a concentration 5%. results showed that for all cultivars, highest number shoots/inoculum obtained medium, maximum value 54.42 ± 4.18 presented by Black’. Considering length shoots, tested cultivars outstanding 5% starch. values regarding observed Thornless’ followed ‘Čačanska Ness’ 5.55 0.04 cm, 5.46 0.06 5.37 0.09 respectively. uniformity micropropagated relation plants confirmed sequence-related amplified polymorphism (SRAP) start codon targeted (SCoT) molecular markers.

Язык: Английский

Biotization with Plant Growth-Promoting Bacteria Benefits the Survival and Production of Potato (Solanum tuberosum L.) In Vitro and In Vivo DOI Creative Commons
Yulimar Castro, Joyce Dória Rodrigues Soares, Ana Milena Gómez Sepúlveda

и другие.

Horticulturae, Год журнала: 2025, Номер 11(4), С. 393 - 393

Опубликована: Апрель 8, 2025

Bacterial inoculation stimulates growth and adaptation in micropropagated plants. This study evaluated the effects of biotization on vitro seedling production vivo two potato cultivars, Agatha Duvira. Nine bacterial strains were tested for hormone ACC deaminase activity then inoculated re-inoculated vivo. Growth, adaptation, tuber assessed. Biotization significantly enhanced growth, survival, yield. Biotized seedlings had a 1.3-fold higher survival rate than controls. Azospirillum brasilense Ab-V5 Rhizobium tropici CIAT 899 promoted at least one variable both cultivars under conditions. A. consistently improved plant performance across stages, with plants showing 1.2–1.3-fold increases stem root length 1.1-fold gain total dry biomass. Additionally, produced 1.9 times more tubers strain-dependent, improving quality enhancing survivability

Язык: Английский

Процитировано

0

Evaluating the Potential of Rhizobacterial Isolates Enhancing Sorghum's Abiotic Stress Tolerance: A Focus on Ethiopian Soil Isolates From Sorghum Root DOI

Mekdes Mulugeta,

Daniel Yimer,

Tilahun Rabuma

и другие.

Journal of Agronomy and Crop Science, Год журнала: 2025, Номер 211(3)

Опубликована: Апрель 15, 2025

ABSTRACT Sorghum is an economically important crop for human consumption, animal feed and biomass production. However, its productivity affected by abiotic biotic stresses. Drought one of the major global problems due to alarming climate change. Plant growth‐promoting rhizobacteria (PGPR) help crops improve their resilience survival in water‐scarce environments. Therefore, present study aimed investigate potential rhizobacterial isolates sorghum tolerance drought other stress. The bacterial sample from different soil roots genotype was isolated locations Ethiopia using serial dilution techniques. biochemical properties these were evaluated. PGPR traits analysed under temperatures, pH, salinity Furthermore, isolates' performance evaluated four concentrations PEG (6000): (10%, 15%, 25% 32.6%), which induces osmotic stress reducing water availability, thus mimicking effects sorghum. PCR detection genes associated with stress, such as phosphate solubilisation, nitrogen fixation, ACC (1‐aminocyclopropane‐1‐carboxylate) deaminase phytohormone production performed designed primers. In addition, identification molecular characterisation PGPRs 16S ribosomal RNA (rRNA) gene‐specific Serial techniques resulted 210 isolates. Biochemical analysis revealed that 68 exhibited while 50 showed ability solubilise phosphate. amplification identified involved several isolates, suggesting they have tolerance. Among examined, nifH gene 16 acdS 10 pgg 21 these, Pseudomonas ms22 isolate a high promote growth greenhouse performance. our findings suggest harnessing could pave way environmentally friendly efficient agricultural practices conditions.

Язык: Английский

Процитировано

0

Bambara groundnut: a neglected and underutilized climate-resilient crop with great potential to alleviate food insecurity in sub-Saharan Africa DOI
Abdoulaye Soumaré, Abdala Gamby Diédhiou, Aboubacry Kane

и другие.

Journal of Crop Improvement, Год журнала: 2021, Номер 36(5), С. 747 - 767

Опубликована: Ноя. 16, 2021

Bambara groundnut (Vigna subterranea) is a drought-tolerant legume in sub-Saharan Africa and well adapted to marginal agricultural lands. Its seeds have high nutritional value, owing their protein, fat, fiber, carbohydrate, vitamin, mineral element contents. Nevertheless, this crop still underutilized, not well-known has almost been ignored by researchers funding bodies. Meanwhile, offers opportunities for diversification great potential significantly contribute food security, especially the context of climate change. This paper provides an overview origin, distribution, adaptation resilience change groundnut, as constraints production its diffusion. Furthermore, review highlights advantages offered micropropagation, microbial biofertilizers plant breeding improve yield. Hence, useful information tools fully exploit groundnut.

Язык: Английский

Процитировано

24

Contribution of yeast and its biomass for the preparation of industrially essential materials: A boon to circular economy DOI
Atif Khurshid Wani, Farida Rahayu, Ita Yustina

и другие.

Bioresource Technology Reports, Год журнала: 2023, Номер 23, С. 101508 - 101508

Опубликована: Июнь 16, 2023

Язык: Английский

Процитировано

10

The Use of Wheat Starch as Gelling Agent for In Vitro Proliferation of Blackberry (Rubus fruticosus L.) Cultivars and the Evaluation of Genetic Fidelity after Repeated Subcultures DOI Creative Commons
Doina Clapa, Monica Hârța, Katalin Szabo

и другие.

Horticulturae, Год журнала: 2023, Номер 9(8), С. 902 - 902

Опубликована: Авг. 8, 2023

Micropropagation has an important role in the large-scale production of blackberry plant material, given high proliferation rates this species. The aim present study was to evaluate proliferative capacity grown vitro on wheat starch-gelled culture medium compared classical agar-gelled and assess genetic fidelity between proliferated shoots their mother plants. Six varieties (‘Čačanska Bestrna’, ‘Chester Thornless’, ‘Driscoll’s Victoria’, ‘Loch Ness’, ‘Polar’, ‘Karaka Black’) were tested. For proliferation, Murashige Skoog (MS) supplemented with 0.5 mg dm−3 6-benzyladenine (BA) used. conventional gelled 0.5% agar, starch used as alternative gelling agent a concentration 5%. results showed that for all cultivars, highest number shoots/inoculum obtained medium, maximum value 54.42 ± 4.18 presented by Black’. Considering length shoots, tested cultivars outstanding 5% starch. values regarding observed Thornless’ followed ‘Čačanska Ness’ 5.55 0.04 cm, 5.46 0.06 5.37 0.09 respectively. uniformity micropropagated relation plants confirmed sequence-related amplified polymorphism (SRAP) start codon targeted (SCoT) molecular markers.

Язык: Английский

Процитировано

9