The
deep
biosphere
is
the
largest
microbial
habitat
on
Earth
and
features
abundant
bacterial
endospores.
Whereas
dormancy
survival
at
theoretical
energy
minima
are
hallmarks
of
physiology
in
subsurface,
ecological
processes
such
as
dispersal
selection
remain
poorly
understood.
We
investigated
biogeography
dispersing
bacteria
sea
where
upward
hydrocarbon
seepage
was
confirmed
by
acoustic
imagery
geochemistry.
Thermophilic
endospores
permanently
cold
seabed
correlated
with
underlying
seep
conduits
reveal
geofluid-facilitated
cell
migration
pathways
originating
petroleum-bearing
sediments.
Endospore
genomes
highlight
adaptations
to
life
anoxic
petroleum
systems
bear
close
resemblance
oil
reservoir
microbiomes
globally.
Upon
transport
out
viable
thermophilic
reenter
geosphere
sediment
burial,
enabling
germination
environmental
depth
new
establish.
This
loop
circulates
living
biomass
biosphere.
Cyanobacteria
and
eukaryotic
phytoplankton
produce
long-chain
alkanes
generate
around
100
times
greater
quantities
of
hydrocarbons
in
the
ocean
compared
to
natural
seeps
anthropogenic
sources.
Yet,
these
compounds
do
not
accumulate
water
column,
suggesting
rapid
biodegradation
by
co-localized
microbial
populations.
Despite
their
ecological
importance,
identities
microbes
involved
this
cryptic
hydrocarbon
cycle
are
mostly
unknown.
Here,
we
identified
genes
encoding
enzymes
across
salinity
gradient
a
remote,
vertically
stratified,
seawater-containing
High
Arctic
lake
that
is
isolated
from
petroleum
sources
seeps.
Metagenomic
analysis
revealed
diverse
cycling
populations,
with
patterns
variation
along
gradients
light,
salinity,
oxygen,
sulfur
relevant
freshwater,
oceanic,
hadal,
anoxic
deep
sea
ecosystems.
Canadian Journal of Microbiology,
Год журнала:
2024,
Номер
70(5), С. 163 - 177
Опубликована: Фев. 13, 2024
Global
warming-induced
sea
ice
loss
in
the
Canadian
Northwest
Passage
(NWP)
will
result
more
shipping
traffic,
increasing
risk
of
oil
spills.
Microorganisms
inhabiting
NWP
beach
sediments
may
degrade
hydrocarbons,
offering
a
potential
bioremediation
strategy.
In
this
study,
characterization
and
genomic
analyses
22
hydrocarbon-biodegradative
bacterial
isolates
revealed
that
they
contained
diverse
range
key
alkane
aromatic
hydrocarbon-degradative
genes,
as
well
cold
salt
tolerance
genes
indicating
are
highly
adapted
to
extreme
Arctic
environment.
Some
successfully
degraded
Ultra
Low
Sulfur
Fuel
Oil
(ULSFO)
at
temperatures
low
−5
°C
high
salinities
(3%–10%).
Three
were
grown
liquid
medium
containing
ULSFO
sole
carbon
source
over
3
months
variation
hydrocarbon
concentration
was
measured
three
time
points
determine
their
rate
biodegradation.
Our
results
demonstrate
two
(
Rhodococcus
sp.
R1B_2T
Pseudarthrobacter
R2D_1T)
possess
complete
degradation
pathways
can
grow
on
components
under
conditions.
Overall,
these
hydrocarbon-degrading
microorganisms
exist
sediments,
strategy
events
marine
fuel
spill
reaching
shores
NWP.
Disturbances
cause
rapid
changes
to
forests,
with
different
disturbance
types
and
severities
creating
unique
ecosystem
trajectories
that
can
impact
the
underlying
soil
microbiome.
Pile
burning-the
combustion
of
logging
residue
on
forest
floor-is
a
common
fuel
reduction
practice
have
impacts
soils
analogous
those
following
high-severity
wildfire.
Further,
pile
burning
clear-cut
harvesting
create
persistent
openings
dominated
by
nonwoody
plants
surrounded
dense
regenerating
conifer
forest.
A
paired
60-year
chronosequence
burn
scar
surrounding
after
provides
opportunity
assess
whether
belowground
microbial
processes
mirror
aboveground
vegetation
during
disturbance-induced
shifts.
Soil
ectomycorrhizal
fungal
diversity
was
reduced
first
decade
burning,
which
could
explain
poor
tree
seedling
establishment
subsequent
persistence
herbaceous
species
within
openings.
Fine-scale
in
microbiome
mirrored
shifts
vegetation,
short-term
carbon
cycling
functions
resembling
postfire
(e.g.
enrichment
aromatic
degradation
genes)
respiration
scars
decoupled
from
substrate
quantity
quality.
Broadly,
however,
composition
function
converged
six
decades
disturbances,
indicating
potential
resilience
disconnected
This
work
begins
unravel
underlie
changes,
are
increasing
frequency
tied
climate
change.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 8, 2024
Oil
reservoirs,
being
one
of
the
significant
subsurface
repositories
energy
and
carbon,
host
diverse
microbial
communities
affecting
production
carbon
emissions.
Viruses
play
crucial
roles
in
ecology
microbiomes,
however,
their
distribution
ecological
significance
oil
reservoirs
remain
undetermined.
Here,
we
assemble
a
catalogue
encompassing
viral
prokaryotic
genomes
sourced
from
reservoirs.
The
comprises
7229
3,886
Operational
Taxonomic
Units
(vOTUs)
182
reservoir
metagenomes.
results
show
that
viruses
are
widely
distributed
85%
vOTUs
detected
less
than
10%
samples,
highlighting
heterogeneous
nature
within
Through
combined
microcosm
enrichment
experiments
bioinformatics
analysis,
validate
regulating
community
structure
sulfate
reducing
microorganisms,
primarily
through
virulent
lifestyle.
Taken
together,
this
study
uncovers
rich
diversity
functions
offering
comprehensive
understanding
role
biogeochemical
cycles
deep
biosphere.
authors
use
metagenomics
data
to
construct
characterize
world-wide,
further
an
microorganisms.
Microorganisms,
Год журнала:
2025,
Номер
13(2), С. 222 - 222
Опубликована: Янв. 21, 2025
Marine
cold
seeps
are
unique
ecological
niches
characterized
by
the
emergence
of
hydrocarbons,
including
methane,
which
fosters
diverse
microbial
communities.
This
study
investigates
diversity
and
distribution
hydrocarbon-degrading
genes
organisms
in
sediments
from
Caspian
Mediterranean
Seas,
utilizing
16S
rRNA
metagenomic
sequencing
to
elucidate
community
structure
functional
potential.
Our
findings
reveal
distinct
differences
hydrocarbon
degrading
gene
profiles
between
two
seas,
with
pathways
for
aerobic
anaerobic
degradation
co-existing
both
basins.
Aerobic
predominate
surface
Sea,
while
favored
anoxic
Sea.
Additionally,
sediment
depths
significantly
influence
diversity,
variations
abundance
composition
observed
at
different
depths.
decrease
depth
whereas
increases
These
results
enhance
our
understanding
ecology
seep
environments
have
implications
bioremediation
practices
targeting
pollutants
marine
ecosystems.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 5, 2025
Since
the
beginning
of
their
production
and
use,
fossil
fuels
have
affected
ecosystems,
causing
significant
damage
to
biodiversity.
Bacterial
bioremediation
can
provide
solutions
this
environmental
problem.
In
study,
new
species
Isoptericola
peretonis
sp.
nov.
4D.3
T
has
been
characterized
compared
other
closely
related
in
terms
hydrocarbon
degradation
biosurfactant
by
vitro
silico
analyses.
Biosurfactants
play
an
important
role
microbial
emulsifying
hydrocarbons
making
them
accessible
machinery.
The
tests
performed
showed
positive
results
a
greater
or
lesser
degree
for
all
strains.
synthesis
biosurfactants,
strains
tested
activity
three
complementary
assays
(CTAB,
hemolysis
E
24
%)
rhamnolipid
genes
predicted
majority
Regarding
degradation,
analyzed
presented
putative
responsible
aerobic
anaerobic
aromatic
alkane
hydrocarbons.
Overall,
our
highlight
metabolic
diversity
biochemical
robustness
genus
which
is
proposed
be
interest
field
bioremediation.
Microbiology Resource Announcements,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 4, 2025
ABSTRACT
We
present
the
complete
genome
sequences
of
nine
bacterial
strains
isolated
from
microplastics
water
or
sediments
Bow
River
in
Calgary,
Alberta.
These
isolates
provide
insight
into
freshwater
microplastic
microbiome
and
their
plastic
biodegradation
potential.
FEMS Microbiology Ecology,
Год журнала:
2023,
Номер
99(12)
Опубликована: Ноя. 13, 2023
Abstract
Naphthenic
acids
(NAs)
are
a
complex
mixture
of
organic
compounds
released
during
bitumen
extraction
from
mined
oil
sands
that
important
contaminants
process-affected
water
(OSPW).
NAs
can
be
toxic
to
aquatic
organisms
and,
therefore,
main
target
compound
for
OSPW.
The
ability
microorganisms
degrade
exploited
bioremediation
OSPW
using
constructed
wetland
treatment
systems
(CWTS),
which
represent
possible
low
energy
and
low-cost
option
scalable
in
situ
NA
removal.
Recent
advances
genomics
analytical
chemistry
have
provided
insights
into
better
understanding
the
metabolic
pathways
genes
involved
degradation.
Here,
we
discuss
ecology
microbial
degradation
with
focus
on
CWTS
summarize
current
knowledge
related
used
by
NAs.
Evidence
date
suggests
mostly
degraded
aerobically
through
ring
cleavage
via
beta-oxidation
pathway,
combined
other
steps
such
as
aromatization,
alpha-oxidation,
omega-oxidation,
or
activation
coenzyme
A
(CoA)
thioesters.
Anaerobic
has
also
been
reported
production
benzoyl-CoA
an
intermediate
and/or
involvement
methanogens
nitrate,
sulfate,
iron
reducers.
Furthermore,
how
genomic,
statistical,
modeling
tools
assist
development
improved
practices.
