Effects of marine diesel on microbial diversity and activity in high Arctic beach sediments DOI Creative Commons
Margaux Durand, David Touchette, Ya-Jou Chen

et al.

Marine Pollution Bulletin, Journal Year: 2023, Volume and Issue: 194, P. 115226 - 115226

Published: July 11, 2023

Global warming induced sea ice loss increases Arctic maritime traffic, enhancing the risk of ecosystem contamination from fuel spills and nutrient loading. The impact marine diesel on bacterial metabolic activity diversity, assessed by colorimetric assay, 16S rRNA metagenomic sequencing, Northwest Passage (Arctic Ocean) beach sediments was with amendment at environmentally relevant temperatures (5 15 °C). Higher temperature nutrients stimulated microbial activity, while reduced it, metabolism inhibited above 0.01 % (without nutrients) 1 (with inclusions. Diesel exposure significantly decreased diversity selected for Psychrobacter genus. Microbial hydrocarbon degradation, organic compound metabolism, exopolysaccharide production gene abundances increased under higher concentrations. Metagenomic binning recovered nine MAGs/bins degradation genes. We demonstrate a nutrients' rescue-type effect in contaminated communities via enrichment microorganisms stress response, aromatic compound, ammonia assimilation metabolisms.

Language: Английский

Geological processes mediate a microbial dispersal loop in the deep biosphere DOI Creative Commons
Daniel A. Gittins, Pierre‐Arnaud Desiage,

Natasha Morrison

et al.

Science Advances, Journal Year: 2022, Volume and Issue: 8(34)

Published: Aug. 26, 2022

The deep biosphere is the largest microbial habitat on Earth and features abundant bacterial endospores. Whereas dormancy survival at theoretical energy minima are hallmarks of physiology in subsurface, ecological processes such as dispersal selection remain poorly understood. We investigated biogeography dispersing bacteria sea where upward hydrocarbon seepage was confirmed by acoustic imagery geochemistry. Thermophilic endospores permanently cold seabed correlated with underlying seep conduits reveal geofluid-facilitated cell migration pathways originating petroleum-bearing sediments. Endospore genomes highlight adaptations to life anoxic petroleum systems bear close resemblance oil reservoir microbiomes globally. Upon transport out viable thermophilic reenter geosphere sediment burial, enabling germination environmental depth new establish. This loop circulates living biomass biosphere.

Language: Английский

Citations

27
Genomic insights into cryptic cycles of microbial hydrocarbon production and degradation in contiguous freshwater and marine microbiomes DOI Creative Commons
Adrien Vigneron, Perrine Cruaud, Connie Lovejoy

et al.

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: May 12, 2023

Cyanobacteria and eukaryotic phytoplankton produce long-chain alkanes generate around 100 times greater quantities of hydrocarbons in the ocean compared to natural seeps anthropogenic sources. Yet, these compounds do not accumulate water column, suggesting rapid biodegradation by co-localized microbial populations. Despite their ecological importance, identities microbes involved this cryptic hydrocarbon cycle are mostly unknown. Here, we identified genes encoding enzymes across salinity gradient a remote, vertically stratified, seawater-containing High Arctic lake that is isolated from petroleum sources seeps. Metagenomic analysis revealed diverse cycling populations, with patterns variation along gradients light, salinity, oxygen, sulfur relevant freshwater, oceanic, hadal, anoxic deep sea ecosystems.

Language: Английский

Citations

14
Broad-spectrum hydrocarbon-degrading microbes in the global ocean metagenomes DOI
Qing Liu, Yongyi Peng,

Jing Liao

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 926, P. 171746 - 171746

Published: March 21, 2024

Language: Английский

Citations

5
Characterization of hydrocarbon degraders from Northwest Passage beach sediments and assessment of their ability for bioremediation DOI
Antoine-Olivier Lirette, Ya-Jou Chen, Nastasia J. Freyria

et al.

Canadian Journal of Microbiology, Journal Year: 2024, Volume and Issue: 70(5), P. 163 - 177

Published: Feb. 13, 2024

Global warming-induced sea ice loss in the Canadian Northwest Passage (NWP) will result more shipping traffic, increasing risk of oil spills. Microorganisms inhabiting NWP beach sediments may degrade hydrocarbons, offering a potential bioremediation strategy. In this study, characterization and genomic analyses 22 hydrocarbon-biodegradative bacterial isolates revealed that they contained diverse range key alkane aromatic hydrocarbon-degradative genes, as well cold salt tolerance genes indicating are highly adapted to extreme Arctic environment. Some successfully degraded Ultra Low Sulfur Fuel Oil (ULSFO) at temperatures low −5 °C high salinities (3%–10%). Three were grown liquid medium containing ULSFO sole carbon source over 3 months variation hydrocarbon concentration was measured three time points determine their rate biodegradation. Our results demonstrate two ( Rhodococcus sp. R1B_2T Pseudarthrobacter R2D_1T) possess complete degradation pathways can grow on components under conditions. Overall, these hydrocarbon-degrading microorganisms exist sediments, strategy events marine fuel spill reaching shores NWP.

Language: Английский

Citations

4
Soil microbiome feedbacks during disturbance-driven forest ecosystem conversion DOI Creative Commons
Amelia R. Nelson, Timothy S. Fegel, Robert Danczak

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Disturbances cause rapid changes to forests, with different disturbance types and severities creating unique ecosystem trajectories that can impact the underlying soil microbiome. Pile burning-the combustion of logging residue on forest floor-is a common fuel reduction practice have impacts soils analogous those following high-severity wildfire. Further, pile burning clear-cut harvesting create persistent openings dominated by nonwoody plants surrounded dense regenerating conifer forest. A paired 60-year chronosequence burn scar surrounding after provides opportunity assess whether belowground microbial processes mirror aboveground vegetation during disturbance-induced shifts. Soil ectomycorrhizal fungal diversity was reduced first decade burning, which could explain poor tree seedling establishment subsequent persistence herbaceous species within openings. Fine-scale in microbiome mirrored shifts vegetation, short-term carbon cycling functions resembling postfire (e.g. enrichment aromatic degradation genes) respiration scars decoupled from substrate quantity quality. Broadly, however, composition function converged six decades disturbances, indicating potential resilience disconnected This work begins unravel underlie changes, are increasing frequency tied climate change.

Language: Английский

Citations

4
Global diversity and ecological functions of viruses inhabiting oil reservoirs DOI Creative Commons

Liyun An,

Xinwu Liu, Jianwei Wang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 8, 2024

Oil reservoirs, being one of the significant subsurface repositories energy and carbon, host diverse microbial communities affecting production carbon emissions. Viruses play crucial roles in ecology microbiomes, however, their distribution ecological significance oil reservoirs remain undetermined. Here, we assemble a catalogue encompassing viral prokaryotic genomes sourced from reservoirs. The comprises 7229 3,886 Operational Taxonomic Units (vOTUs) 182 reservoir metagenomes. results show that viruses are widely distributed 85% vOTUs detected less than 10% samples, highlighting heterogeneous nature within Through combined microcosm enrichment experiments bioinformatics analysis, validate regulating community structure sulfate reducing microorganisms, primarily through virulent lifestyle. Taken together, this study uncovers rich diversity functions offering comprehensive understanding role biogeochemical cycles deep biosphere. authors use metagenomics data to construct characterize world-wide, further an microorganisms.

Language: Английский

Citations

4
Diversity and Distribution of Hydrocarbon-Degrading Genes in the Cold Seeps from the Mediterranean and Caspian Seas DOI Creative Commons

Yogita Warkhade,

Laura G. Schaerer,

Isaac Bigcraft

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 222 - 222

Published: Jan. 21, 2025

Marine cold seeps are unique ecological niches characterized by the emergence of hydrocarbons, including methane, which fosters diverse microbial communities. This study investigates diversity and distribution hydrocarbon-degrading genes organisms in sediments from Caspian Mediterranean Seas, utilizing 16S rRNA metagenomic sequencing to elucidate community structure functional potential. Our findings reveal distinct differences hydrocarbon degrading gene profiles between two seas, with pathways for aerobic anaerobic degradation co-existing both basins. Aerobic predominate surface Sea, while favored anoxic Sea. Additionally, sediment depths significantly influence diversity, variations abundance composition observed at different depths. decrease depth whereas increases These results enhance our understanding ecology seep environments have implications bioremediation practices targeting pollutants marine ecosystems.

Language: Английский

Citations

0
Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative study DOI Creative Commons
Àngela Vidal‐Verdú, Adriel Latorre‐Pérez,

Javier Pascual

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 5, 2025

Since the beginning of their production and use, fossil fuels have affected ecosystems, causing significant damage to biodiversity. Bacterial bioremediation can provide solutions this environmental problem. In study, new species Isoptericola peretonis sp. nov. 4D.3 T has been characterized compared other closely related in terms hydrocarbon degradation biosurfactant by vitro silico analyses. Biosurfactants play an important role microbial emulsifying hydrocarbons making them accessible machinery. The tests performed showed positive results a greater or lesser degree for all strains. synthesis biosurfactants, strains tested activity three complementary assays (CTAB, hemolysis E 24 %) rhamnolipid genes predicted majority Regarding degradation, analyzed presented putative responsible aerobic anaerobic aromatic alkane hydrocarbons. Overall, our highlight metabolic diversity biochemical robustness genus which is proposed be interest field bioremediation.

Language: Английский

Citations

0
Complete genome sequences of nine isolates from microplastics in the Bow River, Calgary, Canada DOI Open Access
Kira L. Goff,

Sneha Suresh,

Jonas M. Stadfeld

et al.

Microbiology Resource Announcements, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

ABSTRACT We present the complete genome sequences of nine bacterial strains isolated from microplastics water or sediments Bow River in Calgary, Alberta. These isolates provide insight into freshwater microplastic microbiome and their plastic biodegradation potential.

Language: Английский

Citations

0
Microbial degradation of naphthenic acids using constructed wetland treatment systems: metabolic and genomic insights for improved bioremediation of process-affected water DOI Creative Commons
Paula C. J. Reis, Sara Correa‐García, Julien Tremblay

et al.

FEMS Microbiology Ecology, Journal Year: 2023, Volume and Issue: 99(12)

Published: Nov. 13, 2023

Abstract Naphthenic acids (NAs) are a complex mixture of organic compounds released during bitumen extraction from mined oil sands that important contaminants process-affected water (OSPW). NAs can be toxic to aquatic organisms and, therefore, main target compound for OSPW. The ability microorganisms degrade exploited bioremediation OSPW using constructed wetland treatment systems (CWTS), which represent possible low energy and low-cost option scalable in situ NA removal. Recent advances genomics analytical chemistry have provided insights into better understanding the metabolic pathways genes involved degradation. Here, we discuss ecology microbial degradation with focus on CWTS summarize current knowledge related used by NAs. Evidence date suggests mostly degraded aerobically through ring cleavage via beta-oxidation pathway, combined other steps such as aromatization, alpha-oxidation, omega-oxidation, or activation coenzyme A (CoA) thioesters. Anaerobic has also been reported production benzoyl-CoA an intermediate and/or involvement methanogens nitrate, sulfate, iron reducers. Furthermore, how genomic, statistical, modeling tools assist development improved practices.

Language: Английский

Citations

9