Progress in molecular biology and translational science, Год журнала: 2024, Номер unknown, С. 161 - 183
Опубликована: Янв. 1, 2024
Язык: Английский
Microorganisms, Год журнала: 2024, Номер 12(2), С. 283 - 283
Опубликована: Янв. 29, 2024
Yersinia ruckeri is the cause of hemorrhagic septicemia, known as enteric redmouth disease, in salmonid fish species. This bacterial pathogen can form biofilms on abiotic surfaces aquaculture settings or even themselves, contributing to their persistence aquatic environment. Detection methods for this and other pathogens be time-consuming lack specificity sensitivity, limiting timely monitoring, treatment microbial infections, effective control transmission settings. Rapid sensitive detection nucleic acids crucial an appropriate surveillance pathogens, CRISPR/Cas-based assays have emerged a good alternative since it has been proven useful tool rapid, specific, viruses some bacteria. In study, we explored capability CRISPR/Cas13a system (SHERLOCK) specifically detect both DNA RNA (gene transcripts) from planktonic biofilm samples Y. ruckeri. The assay was designed gyrA gene small noncoding RNAs (sRNAs) MicA RprA cultures prepared marine broth. specific crRNA these targets included 28 nt sequence, scaffold sequence necessary Cas13-binding. For all assays, obtained were previously subjected isothermal amplification with recombinase polymerase (RPA) method subsequent T7 transcription RPA amplicons. Finally, by means reporter signal released Cas13a collateral cleavage triggered upon target recognition, measured fluorescence- lateral-flow-based readouts. CRISPR/Cas13a-based able sRNAs samples, sensitivity comparable that qPCR analysis, highlighting potential applicability surveillance.
Язык: Английский
Процитировано
3
Biology, Год журнала: 2024, Номер 13(3), С. 182 - 182
Опубликована: Март 12, 2024
Mosquitoes are the most notorious arthropod vectors of viral and parasitic diseases for which approximately half world’s population, ~4,000,000,000, is at risk. Integrated pest management programs (IPMPs) have achieved some success in mitigating regional transmission persistence these diseases. However, as many vector-borne remain pervasive, it obvious that IPMP successes not been absolute eradicating threat imposed by mosquitoes. Moreover, expanding mosquito geographic ranges caused factors related to climate change globalization (travel, trade, migration), evolution resistance synthetic pesticides, present ongoing challenges reducing or eliminating local global burden diseases, especially economically medically disadvantaged societies. Abatement strategies include control vector populations with pesticides eco-friendly technologies. These “green” technologies SIT, IIT, RIDL, CRISPR/Cas9 gene drive, biological specifically targets aquatic larval stages Regarding latter, effective continues be widespread use Lysinibacillus sphaericus (Ls) Bacillus thuringiensis subsp. israelensis (Bti). Here, we a review health issues elicited mosquitoes, strategies, lastly, focus on biology Ls Bti, an emphasis no has observed field.
Язык: Английский
Процитировано
3
Journal of Nucleic Acids, Год журнала: 2024, Номер 2024(1)
Опубликована: Янв. 1, 2024
The development of sensitive and specific diagnostic tools for hepatitis B virus (HBV) C (HCV) remains crucial effective disease management control. In this study, we utilized CRISPR‐Cas12 CRISPR‐Cas13 systems the detection HBV (DNA virus) HCV (RNA virus), respectively. We designed tested multiple guide RNAs (gRNAs) targeting both viruses, confirming successful cleavage target sequences through gel electrophoresis a fluorescent reporter assay. Using optimized gRNAs, developed lateral flow assay (LFA) HCV, demonstrating concentration‐dependent signal increase. Importantly, no cross‐reactivity was observed with other viral targets. To further enhance sensitivity, employed dual‐enzyme approach, combining Cas12 Cas13 in single reaction, which significantly improved limits viruses. Finally, dual antigen LFA strip capable simultaneously detecting sample. This approach holds promise point‐of‐care (POC) diagnostics where infection is unknown. study addresses current limitations CRISPR‐Cas based diagnostics, namely, need ultrasensitive methods ability to detect antigens using test strip. Our findings demonstrate feasibility highly paving way potential POC application.
Язык: Английский
Процитировано
3
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13321 - 13321
Опубликована: Дек. 12, 2024
CRISPR–Cas technology has transformed the field of gene editing, opening new possibilities for treatment various genetic disorders. Recent years have seen a surge in clinical trials using CRISPR–Cas-based therapies. This review examines current landscape implementation trials, with data from key registries, including Australian New Zealand Clinical Trials Registry, Chinese Trial Register, and ClinicalTrials.gov. Emphasis is placed on mechanism action tested therapies, delivery method, most recent findings each trial.
Язык: Английский
Процитировано
2
Acta Biochimica et Biophysica Sinica, Год журнала: 2023, Номер 55(10), С. 1539 - 1550
Опубликована: Июль 11, 2023
CRISPR-based detection technologies have been widely explored for molecular diagnostics. However, the challenge lies in converting signal of different biomolecules, such as nucleic acids, proteins, small molecules, exosomes, and ions, into a acid signal. Understanding biomolecules using CRISPR technology can aid development practical promising approaches. Unfortunately, existing reviews rarely provide an overview diagnostics from perspective biomolecules. Herein, we first introduce principles characteristics various nucleases Then, focus on summarizing evaluating latest advancements Through comparison methods amplification readout, discuss how general be integrated with CRISPR. Finally, conclude by identifying opportunities improvement quantitative, amplification-free, multiplex, all-in-one, point-of-care testing (POCT) purposes.
Язык: Английский
Процитировано
4
Theoretical and Natural Science, Год журнала: 2024, Номер 33(1), С. 6 - 11
Опубликована: Март 7, 2024
Myopia is a relatively common disease at present, especially in the adolescent population very high, and its prevalence increases with passage of time, usually myopia will cause retinal detachment, glaucoma, etc., which may seriously lead to vision loss blindness. Existing treatments include monocular lenses, contact lenses refractive surgery, as well drugs atropine pirrenzil. Studies have shown that most little effect, are short term, significant side effects. This article mainly summarizes treatment methods myopia, combined latest findings clinical research, put forward possible future methods.
Язык: Английский
Процитировано
1
Progress in molecular biology and translational science, Год журнала: 2024, Номер unknown, С. 1 - 17
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
1
Molecular Biotechnology, Год журнала: 2024, Номер unknown
Опубликована: Окт. 24, 2024
Язык: Английский
Процитировано
1
Cells, Год журнала: 2024, Номер 13(21), С. 1794 - 1794
Опубликована: Окт. 30, 2024
Since the onset of COVID-19 pandemic, a variety diagnostic approaches, including RT-qPCR, RAPID, and LFA, have been adopted, with RT-qPCR emerging as gold standard. However, significant challenge in diagnostics is wide range symptoms presented by patients, necessitating early accurate diagnosis for effective management. Although precise molecular technique, it not immune to false-negative results. In contrast, CRISPR-based detection methods SARS-CoV-2 offer several advantages: they are cost-effective, time-efficient, highly sensitive, specific, do require sophisticated instruments. These also show promise scalability, enabling tests. CRISPR technology can be customized target any genomic region interest, making versatile tool applications beyond diagnostics, therapeutic development. The CRISPR/Cas systems provide gene targeting immense potential creating next-generation therapeutics. One key advantages CRISPR/Cas-based therapeutics ability perform multiplexing, where different sgRNAs or crRNAs multiple sites within same gene, reducing likelihood viral escape mutants. Among various systems, CRISPR/Cas13 CARVER (Cas13-assisted restriction expression readout) particularly promising. broad single-stranded RNA viruses, them suitable treatment diseases, SARS-CoV-2. efficacy safety must thoroughly evaluated pre-clinical clinical settings. While biotechnologies yet fully harnessed control current there an optimism that limitations system overcome soon. This review discusses how strategies revolutionize disease development, better preparing us future threats.
Язык: Английский
Процитировано
1
Deleted Journal, Год журнала: 2024, Номер 1(3), С. 100035 - 100035
Опубликована: Окт. 31, 2024
Язык: Английский
Процитировано
1